Abstract
The accurate determination of the size and depth of infiltration is critical to the treatment and excision of melanoma and other skin cancers. However, current techniques, such as skin biopsy and histological examination, pose invasiveness, time-consumption, and have limitations in measuring at the deepest level. Non-invasive imaging techniques like dermoscopy and confocal microscopy also present limitations in accurately capturing contrast and depth information for various skin types and lesion locations. Thus, there is a pressing need for non-invasive devices capable of obtaining high-resolution 3D images of skin lesions. In this study, we introduce a novel device that combines 18 MHz ultrasound and photoacoustic tomography into a single unit, enabling the acquisition of colocalized 3D images of skin lesions. We performed in vivo measurements on 25 suspicious human skin nevi that were promptly excised following measurements. The combined ultrasound/photoacoustic tomography imaging technique exhibited a strong correlation with histological Breslow thickness between 0.2 and 3 mm, achieving a coefficient of determination (R\(^2\)) of 0.93, which is superior to the coefficients from the individual modalities. The results procured in our study underscore the potential of combined ultrasound and photoacoustic tomography as a promising non-invasive 3D imaging approach for evaluating human nevi and other skin lesions. Furthermore, the system allows for integration of other optical modalities such as optical coherence tomography, microscopy, or Raman spectroscopy in future applications.
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Introduction
The incidence and mortality of skin cancer have been increasing over the past decades, especially in regions with predominantly fair-skinned population and high sun exposure. It is the most common type of cancer worldwide, with more than 1.5 million new cases estimated in 2020. At the same time for melanoma, which is the most dangerous and invasive type of skin cancer, an estimated 325,000 new cases were diagnosed globally and 57,000 deaths resulted from the disease1.
The stage at which melanoma is diagnosed is a major factor in its prognosis, with a 10-year survival rate of 95% for localized melanoma and less than 10% for metastatic melanoma2. Early detection and diagnosis are therefore critical for improving survival rates and reducing associated mortality. Currently, the gold standard for diagnosis is visual examination and biopsy for histopathological analysis if the lesion is deemed suspicious. However, this approach is invasive, costly, and time-consuming, which may result in delayed treatment. Additionally, histopathology analysis only evaluates a couple of slices, which may not accurately represent the lesion’s largest depth and could result in the underestimation of its thickness. This, in turn, could lead to inappropriate tumor staging and clinical procedures. Additionally, preoperative knowledge about size and depth of the lesion would facilitate the planning of the surgery and increase the chance of tumor free excision margins.
As a result, there is a need for alternative fast and non-invasive imaging methods, which would ideally provide the dermatologist with a 3D image of the lesion. Many clinical studies have thus analyzed methods such as optical coherence tomography (OCT)3, hyperspectral imaging4, reflectance confocal5,6, fluorescence7, and multiphoton microscopy8,9, photoacoustic tomography (PAT)10,11, and high-frequency ultrasound imaging (HFUS)12,13,14, or some combination of them15,16. Also, photoacoustic microscopy (PAM) has shown successful applications in dermatology and in particular in angiography17,18,19. Table 1 presents a summary of the advantages and disadvantages of various skin imaging modalities for melanoma assessment. Unfortunately, most purely optical imaging methods are often limited in maximal imaging depth and are, therefore, incapable of measuring the depths for all possible ranges to date. On the other hand, ultrasound and photoacoustic tomography enable resolving deeper lesions, although usually at the price of reduced contrast. However, most demonstrated clinical studies are done with B-mode (2D slices) methods and are therefore subject to the same problem as histopathology.
For this study, we have developed a new system that combines ultrasound (US) and photoacoustic tomography (PAT) in a single scanning unit, which is capable of performing a sequence of equidistant 2D slices. This allows to visualize the lesion in 3D, which helps to localize the deepest position and assist the surgeon in the decision of excision margins. The proposed design has a clear optical window above the lesion, which allows it to be combined with other optical modalities in addition. We conducted an in vivo prospective measurement on 25 suspicious human pigmented skin lesions to validate the system for clinical application. The lesions were promptly excised following the imaging measurements for further histopathological analysis. By correlating the obtained imaging data with the corresponding histological findings, we analyzed the accuracy and potential clinical utility of the combined ultrasound and photoacoustic tomography approach in non-invasive 3D imaging of human melanocytic and potentially other skin lesions.
Materials and methods
Experimental setup
The principle and functionality of the device was presented in our previous work22. The experimental setup is represented in Fig. 1. The ultrasound and photoacoustic reception is done with a 18 MHz central single crystal transducer (L22-14vX-LF, Vermon, France) with an acoustical focal length of 20 mm, see16,22. It is connected to a research ultrasound system (Vantage 32LE, Verasonics, USA), which generates and acquires the signals at a sampling rate of 62.5 MHz. The transducer is positioned horizontally inside a water tank (WT) that is 3D-printed with resin. The bottom of the WT features an opening of 1 cm x 1 cm, which is placed over the lesion and filled with water as acoustic medium. The transducer adapter consists of a glass slab positioned at a 45-degree angle in front of the detector area. Once the WT is filled, the glass functions as an acoustic mirror (AM) and reflects acoustical waves through total internal reflection, folding the imaging plane onto the WT opening. This allows for optical transparency from the top side (which can be utilized for other optical modalities) and from the front side, which is used for photoacoustic tomography (PAT) illumination. The efficiency of acoustic reflection was measured by comparing the US intensity with and without the AM at the same distance. The negligible difference observed suggests a highly efficient reflection rate (greater than 95%).
For 3D imaging, the transducer is translated with a motorized stage (MTS25/M-Z8, Thorlabs, USA) within the WT. The scanning head is mounted onto a standard VESA monitor arm, providing support and enabling convenient alignment of the WT aperture with the skin surface. A photograph of the scanning unit is shown in Fig. 2. The optical excitation for PAT is achieved with an optical parametric oscillator (OPO, SpitLight Compact 400 OPO, InnoLas Laser GmbH, Germany), which generates light pulses of 7 ns duration at 20 Hz repetition rate. The pulses are guided via a custom-made optical fiber bundle (CeramOptec GmbH, Germany) and projected through the frontal optical window of the WT with a collimator consisting of 2 achromatic lenses (AC127-019-A and AC254-030-A, Thorlabs, USA) and a square diffuser (ED1-S20-MD, Thorlabs, USA) onto the opening/lesion. Using highly pigmented coffee agar phantoms that mimic melanin absorption, the maximum imaging depth was found to be at least 5 mm. The spatial resolution capabilities of the modalities are summarized in Table S1. The optical density was kept at \(\le ~6\) mJ/cm\(^2\) per pulse, which is below the legal German28 and American29 Maximum Permissible Exposure (MPE) safety regulations, both of which prescribe 20 mJ/cm\(^2\) per pulse for excitation in the visible range and at 20 Hz repetition rate. We measured each B-mode with US and 2 PAT modes using optical excitation at 430 nm and 530 nm. Every lesion was measured using a sequence of 36 B-modes with a separation of 250 µm between them, and the acquisition time was less than 50 seconds.
Image evaluation
The PAT images are beamformed and reconstructed using the open-source MATLAB library MUST30, which is based on multiplication of quadrature demodulated signals (IQ) with sparse delay-and-sum (DAS) matrix31. For image reconstruction purposes, the speed of sound inside skin tissue was set to 1580 m/s, as suggested by Weichenthal et al.32. To account for variations in skin pigmentation, the dynamic range (logarithmic compression factor) for PAT was automatically determined for each lesion using a MATLAB script that analysed and aimed to achieve a specific pixel histogram distribution.
The US and PAT images were given to several independent researchers as separate stacks in grayscale. Each researcher independently measured the thickness of the lesion at its thickest position within their respective modality’s stack to localize it, similar to the localization of Breslow thickness by a histologist. Subsequently, the largest thickness identified by each researcher, both in the US and PAT images, was considered as the result for the corresponding modality. An example image of the combined US/PAT measurement, illustrating the details of the localization process, is presented in Fig. 3.
Clinical setup
The study recruited patients who had suspicious skin lesions identified by dermatologists as potentially indicative of skin cancer. 19 patients (8 female and 11 male) with Caucasian skin type, with ages between 21 and 77 years, with a total number of 25 such skin lesions that were scheduled for excisions participated in the study. Only lesions that were fully measured with the scanning unit are considered for evaluation. From 25 lesions, 4 were only measured with the US, due to a technical problem with the laser operation. The performed measurements were approved by the Ethics Committee of the University Medical Center Rostock (A 2016-0115) and met the principles of the Declaration of Helsinki. All the participants gave written and oral informed consent for the participation in this study. Immediately after the measurements the lesions were excised, fixed with formalin, paraffin-embedded and stained with haematoxylin and eosin(H &E) for histological examination. A dermatopathologist, blinded to the imaging depth results, provided the histological diagnosis and the Breslow’s depth or the equivalent deepest melanocytic infiltration depth for non-melanoma lesions from the histological slices.
Results and discussion
Figure 4 showcases Bland-Altman plots, a widely employed statistical method for gauging the concordance between two measurement techniques. In these plots, the differences between in vivo measurements and histological depth are visualized against their means. The central horizontal line denotes the mean difference, while the upper and lower lines delineate the limits of agreement, signifying the range wherein 95% of the differences are anticipated to reside.
PAT with 430 nm excitation wavelength has the widest spread and tends to overestimate the thickness. It thus shows the largest confidence interval (CI), which may be due to the poor penetration depth of the radiation at this wavelength. On the other hand, 530 nm PAT and US alone lead to better results with a 95% CI of 0.8 mm. On average, all modalities are overestimating the thickness as compared to the Breslow’s depth by 0.1 to 0.2 mm. The most accurate prediction of histological thickness in terms of the mean and CI is found by averaging the results from US and 530 nm PAT, as shown in the bottom right plot of Fig. 4.
On average the overestimation of the thickness compared to the Breslow measurement ranges from 0.1 mm to 0.2 mm. Notably, other clinical studies with non-invasive methods report similar results12,13,14,33,34. Among the modalities, PAT with 430 nm excitation wavelength exhibits the largest inconsistency with the histological values and is therefore the least reliable. US alone can achieve good results and correlates well with histology, which makes it a more feasible and affordable option for clinical practice. However, PAT can further improve the results by enhancing the contrast and specificity of the imaging. The combination of 530 nm PAT and US shows the strongest correlation with Breslow thickness, with an R\(^2\) of 0.93, as seen in Fig. S2 (supplementary material). Interestingly, these results exhibit a similar pattern of overestimating the thickness for lesions thinner than 0.5 mm and slightly underestimating it for thicker lesions, as reported by other clinical studies using OCT12,14,33, 15-25 MHz US12,13,33,34 and 100 MHz HFUS14. However, at the critical thickness of around 1 mm, which is the cut-off depth to perform sentinel node biopsy, our combined system features a superior performance.
In comparison to other studies with B-mode HFUS14,33 and OCT14, which analysed thinner lesions (\(\le\) 1 mm) due to the partially limited penetration depth of the OCT or HFUS, our study presents measurement on lesions over a larger thickness range (1–3 mm). Also, other studies that investigated thicker lesions in vivo reported CI in the range >2 mm12,20,35, which is significantly above our findings.
In general, the observed overestimation of the modalities as compared to the histological Breslow thickness with regard to thin lesions can be attributed to tissue shrinkage, which occurs immediately after excision and is worsened during the subsequent dehydration and paraffinization stages, resulting in a reduction in histological thickness36,37. It is possible that the shrinkage effect is relative to the size, which is less significant for thicker excisions. Additionally, the histology might underestimate the thickness to some degree due to the fact that the excision slice is not necessarily taken at the location where the lesion has its greatest depth, whereas the used modalities provide a set of B-mode images for analysis. Since the predictions of both US and PAT depend directly on the correct value of the speed of sound, which has shown to have variance (as high as 6%) between individual tumors and even between different sites of normal skin32, it can contribute to further deviations of in vivo measurements with the histological thickness and the observed slight underestimation for thicker lesions.
Furthermore, the proposed imaging with series of equidistant US/PAT scans is especially useful for evaluating very thin lesions with complex shapes, since it can capture size and depth information in three dimensions, as illustrated in Fig. 5. This is important because thin lesions are often difficult to detect and measure with B-mode imaging modalities. Moreover, complex shapes may introduce errors in the estimation of the lesion thickness, as they may not be parallel to the imaging plane or have irregular boundaries. 3D imaging overcomes these limitations by providing a volumetric representation of the lesion that can be sliced and analysed from different angles and perspectives. This allows for a more accurate and reliable assessment of the lesion morphology and dimensions.
A limitation of this study is the relatively small number of skin lesions examined, due to the challenge of finding and recruiting patients. Another limitation is the water leakage from the 1 cm\(^2\) opening of the WT, which prevented some measurements from being conducted on certain body parts. This problem is intended to be solved in the following studies by using a different WT with a smaller opening and measurement area. The current measurement accuracy is not limited by fundamental principles, but mostly determined by the equipment used so far. For the PAT modality, the accuracy and the imaging depth can be increased by excitation at longer wavelengths, for example in the range between 600 - 700 nm. Also, an ultrasound transducer with higher central frequency can be used to increase the spatial resolution. Furthermore, angled illumination of the lesion from different sides can be implemented as opposed to illumination from one side as realized in the current setup. Finally, data analysis and image processing based on deep learning algorithms might be able to further increase accuracy38,39,40.
This study paves the way for several avenues of future research and development. Firstly, further clinical trials involving a larger cohort of patients with various skin types and skin lesions will be carried out to validate the performance and reliability of the combined US and PAT system. This would provide additional evidence of its effectiveness in accurately assessing lesion size and depth, ultimately aiding in clinical decision-making. Moreover, the clear optical window incorporated in the proposed design enables integration of optical modalities such as OCT and Raman spectroscopy41. OCT can provide cross-sectional images of the skin with high resolution, allowing for detailed visualization of tissue structures and potentially aiding in the differentiation of benign and malignant lesions42,43. Raman spectroscopy, on the other hand, can provide molecular information about the lesion, offering insights into its biological composition and assisting in determining its malignancy25,26,27,44. Deep learning-based approaches, such as convolutional neural networks (CNNs), have shown promising results in both classification45 and medical image segmentation tasks43,46. By training a CNN on annotated 3D images, it is possible to develop robust and accurate segmentation models that can automatically delineate the boundaries of skin lesions. This would not only save time and effort for dermatologists but also provide more consistent and objective measurements for evaluating excision margins. Additionally, with the segmentation of the lesion, a 3D representation could directly be displayed to the dermatologist after scanning the lesion for the entire skin area. Overall, the integration of additional optical modalities, the expansion of clinical trials, and the development of automated segmentation techniques hold promise for enhancing the capabilities and clinical utility of the combined US and PAT system in the future.
Conclusion
To summarize, we show that the developed combined measurement system can successfully determine the depth of skin lesions in vivo, while being under regulated MPE levels and with acquisition time of <1 min. The results demonstrate that the method can both accurately measure the size and the depth of skin lesions. We obtained a high level of agreement (R\(^2\) of 0.93, CI of 0.7 mm) between US and PAT, the latter with excitation at 530 nm wavelength, and histopathological measurements, indicating a reliable method for assessing lesion thickness and size before excision. The results are comparable to or better than existing studies with OCT or high frequency ultrasound (HFUS). The approach has the advantage of being non-invasive, fast, and relatively cost-effective, making it suitable for routine clinical use. The combination of the results from the two modalities notably increases accuracy further and allows for improved assessment of infiltration depth and pathophysiology of suspicious lesions, particularly when combined with Raman spectroscopy which is molecular-specific. Moreover, the method can potentially reduce the number of unnecessary biopsies and improve the accuracy of surgical excision planning. Future work will focus on increasing the number of lesions examined and combining the US/PA system with other optical modalities for application in a clinical setting, as well as analyzing the PAT imaging with further laser wavelengths.
Data availability
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
References
Skin cancer statistics|World Cancer Research Fund International (2022). Accessed: 2022-04-04.
Aubuchon, M. et al. Epidemiology, management and survival outcomes of primary cutaneous melanoma: A ten-year overview. Acta Chirurgica Belgica 117, 29–35 (2017).
Abdalla, B. M. Z. et al. Features of an atypical vascular lesion on dermoscopy, reflectance confocal microscopy, and optical coherence tomography. JAAD Case Rep. 35, 8–11 (2023).
Aloupogianni, E., Ishikawa, M., Kobayashi, N. & Obi, T. Hyperspectral and multispectral image processing for gross-level tumor detection in skin lesions: A systematic review. J. Biomed. Opt. 27, 060901–060901 (2022).
Borsari, S. et al. In vivo dermoscopic and confocal microscopy multistep algorithm to detect in situ melanomas. Brit. J. Dermatol. 179, 163–172 (2018).
Guida, S., Pellacani, G., Ciardo, S. & Longo, C. Reflectance confocal microscopy of aging skin and skin cancer. Dermatol. Pract. Concept. 11, 66 (2021).
Niazi, A. et al. Discrimination of normal and cancerous human skin tissues based on laser-induced spectral shift fluorescence microscopy. Sci. Rep. 12, 20927 (2022).
Yew, E., Rowlands, C. & So, P. T. Application of multiphoton microscopy in dermatological studies: A mini-review. J. Innov. Opt. Health Sci. 7, 1330010 (2014).
Elagin, V. et al. In vivo multimodal optical imaging of dermoscopic equivocal melanocytic skin lesions. Sci. Rep. 11, 1–12 (2021).
Kratkiewicz, K. et al. Photoacoustic/ultrasound/optical coherence tomography evaluation of melanoma lesion and healthy skin in a swine model. Sensors 19, 66. https://doi.org/10.3390/s19122815 (2019).
Fedorov Kukk, A., Blumenröther, E. & Roth, B. Self-made transparent optoacoustic detector for measurement of skin lesion thickness in vivo. Biomed. Phys. Eng. Express 8, 035029 (2022).
Meyer, N. et al. High-frequency ultrasonography but not 930-nm optical coherence tomography reliably evaluates melanoma thickness in vivo: A prospective validation study. Brit. J. Dermatol. 171, 799–805 (2014).
Botar-Jid, C. M. et al. Assessment of cutaneous melanoma by use of very-high-frequency ultrasound and real-time elastography. Am. J. Roentgenol. 206, 699–704 (2016).
Varkentin, A. et al. Comparative study of presurgical skin infiltration depth measurements of melanocytic lesions with OCT and high frequency ultrasound. J. Biophoton. 10, 854–861 (2017).
Varkentin, A. et al. Trimodal system for in vivo skin cancer screening with combined optical coherence tomography-Raman and colocalized optoacoustic measurements. J. Biophoton. 11, e201700288 (2018).
Fedorov Kukk, A. et al. Multimodal system for optical biopsy of melanoma with integrated ultrasound, optical coherence tomography and Raman spectroscopy. J. Biophoton. 6, 66 (2022).
Ma, H. et al. Fast linear confocal scanning photoacoustic dermoscopy for non-invasive assessment of chromatodermatosis. Appl. Phys. Lett. 113, 66 (2018).
Ma, H., Cheng, Z., Wang, Z., Zhang, W. & Yang, S. Switchable optical and acoustic resolution photoacoustic dermoscope dedicated into in vivo biopsy-like of human skin. Appl. Phys. Lett. 116, 66 (2020).
Ma, H. et al. Quantitative and anatomical imaging of dermal angiopathy by noninvasive photoacoustic microscopic biopsy. Biomed. Opt. Express 12, 6300–6316 (2021).
Guitera, P. et al. Melanoma histological Breslow thickness predicted by 75-MHz ultrasonography. Brit. J. Dermatol. 159, 364–369 (2008).
Bard, R. L. High-frequency ultrasound examination in the diagnosis of skin cancer. Dermatol. Clin. 35, 505–511 (2017).
Fedorov Kukk, A., Scheling, F., Panzer, R., Emmert, S. & Roth, B. Combined ultrasound and photoacoustic c-mode imaging system for skin lesion assessment. Sci. Rep. 13, 17947 (2023).
Ma, H. et al. Multiscale confocal photoacoustic dermoscopy to evaluate skin health. Quant. Imaging Med. Surg. 12, 2696 (2022).
Gao, Y. et al. 4D spectral-spatial computational photoacoustic dermoscopy. Photoacoustics 34, 100572 (2023).
Feng, X. et al. Raman biophysical markers in skin cancer diagnosis. J. Biomed. Opt. 23, 057002 (2018).
Liu, C.-H. et al. A pilot study for distinguishing basal cell carcinoma from normal human skin tissues using visible resonance Raman spectroscopy. J. Cancer Metastas. Treat. 6, 66 (2019).
Wu, D., Fedorov Kukk, A. & Roth, B. Detection of melanin influence on skin samples based on raman spectroscopy and optical coherence tomography dual-modal approach. J. Biophoton. 6, 66 (2023).
TROS Laserstrahlung Teil 2: Messungen und Berechnungen von Expositionen gegenüber Laserstrahlung (Laser Radiation Part 2: Measurements and Calculations of Exposures to Laser Radiation). Tech. Rep., Bundesanstalt für Arbeitsschutz und Arbeitsmedizin (German Federal Institute for Occupational Safety and Health) (2018).
ANSI Z136.1 Laser Safety Standard for the Safe Use of Lasers. Tech. Rep., Laser Institute of America (2014).
Garcia, D. Make the most of MUST, an open-source Matlab UltraSound Toolbox. In 2021 IEEE International Ultrasonics Symposium (IUS), 1–4 (IEEE, 2021).
Perrot, V., Polichetti, M., Varray, F. & Garcia, D. So you think you can DAS? A viewpoint on delay-and-sum beamforming. Ultrasonics 111, 106309 (2021).
Weichenthal, M., Mohr, P. & Breitbart, E. W. The velocity of ultrasound in human primary melanoma tissue-implications for the clinical use of high resolution sonography. BMC Dermatol. 1, 1–5 (2001).
Hinz, T. et al. Assessment of tumor thickness in melanocytic skin lesions: Comparison of optical coherence tomography, 20-MHz ultrasound and histopathology. Dermatology 223, 161–168 (2011).
Canedo, I. F. et al. Preoperative 15-MHz ultrasound assessment of tumor thickness in malignant melanoma. Actas Dermo-Sifiliográficas (English Edition) 104, 227–231 (2013).
Serrone, L. et al. High frequency ultrasound in the preoperative staging of primary melanoma: A statistical analysis. Melanoma Res. 12, 287–290 (2002).
Kerns, M. J. J. et al. Shrinkage of cutaneous specimens: Formalin or other factors involved?. J. Cutaneous Pathol. 35, 1093–1096 (2008).
Tran, T. et al. Correcting the shrinkage effects of formalin fixation and tissue processing for renal tumors: Toward standardization of pathological reporting of tumor size. J. Cancer 6, 759 (2015).
Antholzer, S., Haltmeier, M. & Schwab, J. Deep learning for photoacoustic tomography from sparse data. Inverse Probl. Sci. Eng. 27, 987–1005 (2019).
Misra, S. et al. Ensemble Transfer Learning of Elastography and B-mode Breast Ultrasound Images. arXiv:2102.08567 (2021).
Shahid, H., Khalid, A., Liu, X., Irfan, M. & Ta, D. A deep learning approach for the photoacoustic tomography recovery from undersampled measurements. Front. Neurosci. 15, 598693 (2021).
Mazurenka, M., Behrendt, L., Meinhardt-Wollweber, M., Morgner, U. & Roth, B. Development of a combined OCT-Raman probe for the prospective in vivo clinical melanoma skin cancer screening. Rev. Sci. Instrum. 88, 105103 (2017).
Rajabi-Estarabadi, A. et al. Optical coherence tomography imaging of melanoma skin cancer. Lasers Med. Sci. 34, 411–420 (2019).
Chuchvara, N., Rao, B. & Liu, X. Manually scanned single fiber optical coherence tomography for skin cancer characterization. Sci. Rep. 11, 1–9 (2021).
Lieber, C. A., Majumder, S. K., Ellis, D. L., Billheimer, D. D. & Mahadevan-Jansen, A. In vivo nonmelanoma skin cancer diagnosis using Raman microspectroscopy. Lasers Surg. Med.: Off. J. Am. Soc. Laser Med. Surg. 40, 461–467 (2008).
Esteva, A. et al. Dermatologist-level classification of skin cancer with deep neural networks. Nature 542, 115–118 (2017).
Ahmed, N., Tan, X. & Ma, L. A new method proposed to melanoma-skin cancer lesion detection and segmentation based on hybrid convolutional neural network. Multimed. Tools Appl. 66, 1–24 (2022).
Acknowledgements
The authors acknowledge financial support from the German Research Foundation DFG (German Research Foundation, Project ID RO 3471/18-1 and EM 63/13-1). Also, financial support from the German Research Foundation (DFG) under Germany’s Excellence Strategy within the Cluster of Excellence PhoenixD (EXC 2122, Project ID 390833453) is acknowledged.
Funding
Open Access funding enabled and organized by Projekt DEAL.
Author information
Authors and Affiliations
Contributions
A.F.K. committed to design setup, script programming, reconstruction and processing of images, clinical measurements and results evaluation, all with the assistance of F.S.; R.P. and S.E. committed to the clinical study, patient recruitment, and histological evaluation. B.R. advised on the setup construction, analysis and interpretation of the data. B.R. and S.E. conceived the research idea, provided all resources and supervised the entire project.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Kukk, A.F., Scheling, F., Panzer, R. et al. Non-invasive 3D imaging of human melanocytic lesions by combined ultrasound and photoacoustic tomography: a pilot study. Sci Rep 14, 2768 (2024). https://doi.org/10.1038/s41598-024-53220-y
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-024-53220-y
- Springer Nature Limited