Abstract
Cropland conversion has a major impact on soil C sequestration. However, it remains unclear about the changes in soil aggregate and their contribution to C accumulation following cropland conversion in a karst region, southwest China. In this study, three different cropland use types (sugarcane, mulberry and forage grass cultivation) were selected to replace maize-soybean cultivation. The soil was collected at a depth of 0 to 30 cm for analysis of soil aggregates and their OC content. Results showed that macro-aggregate was the predominant component underlying four cropland use types. Forage grass cultivation remarkably increased the OC stock and aggregate stability (MWD and GMD). OC content and stock associated with aggregate varied with cropland use types and soil depth, but were typically highest in forage grass fields. Macro-aggregates contained higher OC content and stock than other aggregate fractions, along with soil depth underlying four cropland use types. The increases in OC stock in forage grass field was mainly due to increased OC stocks within macro-aggregates, which is further attributed to the increase in OC content within macro-aggregates. Overall, forage grass cultivation replaced maize-soybean cultivation was suggested as an ecological restoration model to enhance soil C sequestration potential, owing to its role in increasing OC stock of aggregation and aggregate stability, in the karst region of southwest China.
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Discover the latest articles, news and stories from top researchers in related subjects.Introduction
Soil is the largest carbon sink in terrestrial ecosystems, which is twice or three times as much as atmospheric and vegetation carbon (C) pool1. Accordingly, slight C losses from soil may lead to dramatic changes in atmospheric carbon dioxide level and subsequently accelerate global climate change1,2. Intensive agriculture based on cropland management practices is deteriorating soil quality, resulting in soil structure destruction, nutrient loss, and soil erosion3. Moreover, they can reduce soil C sequestration and accelerate greenhouse gas emissions4,5. Therefore, it is imperative to develop new restoration types to replace traditional intensive agriculture and reverse the process of soil C loss6,7,8.
Soil aggregation is a physical process that protects soil organic carbon (OC) by providing barriers between OC and OC decomposer, thus affecting the OC distribution and stability thereof8,9,10. Based on hierarchical model, soil aggregates consist of many fractions, mainly including macro-aggregates (> 0.25 mm) and micro-aggregates (< 0.25 mm)11,12. Generally, macro-aggregates formed from carbohydrate-rich plant residues and associated with soil organic matter (OM) increases13,14. Nevertheless, the soil OC within micro-aggregates decomposes slowly, which leads to a greater protection from microbial breakdown and ultimately to long-term C sequestration15. Thus, the formation of soil aggregates and soil C sequestration are mutually promoted processes16,17. Several studies have reported that soil C sequestration through soil aggregation responds to land use change more quickly than soil C within bulk soil18,19,20. In agriculture erosion land, cropland conversion or restoration, often considered as an effective strategy to recuperate soil productivity and quality through enhancing soil aggregate stability and promoting soil C sequestration21. Furthermore, soil aggregate stability was an important indicator of soil physical quality, which could determine the magnitude and direction of soil C accumulation3,19. Therefore, understanding the pattern of soil aggregates following cropland conversion can provide insights into soil C sequestration and thus provide better land restoration strategies in degraded areas.
Soil OC stabilization in soil aggregate is the principal mechanism for soil C sequestration14. However, the contribution of associated-aggregate OC to bulk soil C accumulation or losses following cropland use change was not thoroughly understood. Soil aggregates are generally classified into macro-agggregate (> 2 mm), meso-aggregate (2–0.25 mm), micro-aggregate (< 0.25 mm) or silt–clay (< 0.053 mm) based on their size22, but they contributed to total soil C sequestration vary differently following cropland use change19,23. For example, Gelaw et al. (2015)23 reported that a 68.2% OC increase in afforested soil is occluded by soil macro-aggregates. While Zhong et al. (2019)19 demonstrated that soil OC stocks in meso-aggregte accounted for 46% of the total increase in bulk OC stocks when abandoned farmland 42 years ago converted to Robinia pseudoacacia with a combined 227.79% increase in total soil OC stocks at 0–20 cm depth. Furthermore, higher C mineralization rate has been observed in larger aggregate fractions than in smaller aggregate fractions24,25. Accordingly, the inconsistent conclusions of individual studies have added to the confusion surrounding the mechanisms that underlie the contribution of aggregates to total soil OC accumulation. Thus, it is essential to clarify which size of aggregates, and whether the aggregate amounts or the OC content associated with a specific aggregate fraction, are dominant modulators of OC accumulation in degraded agriculture ecosystems.
The karst area is widely distributed in the world and accounts for almost 12% of the earth’s land. In China, karst landscape covers about 0.55 million km2 in the southwest region26. Karst ecosystem was notorious for its role in land degradation, such as soil OM losses, soil degradation, and rocky desertification27. In the past decades, maize-soybean rotation (MS) was largely implemented in the karst region, which caused severe soil C loss and soil erosion28. Therefore, it is undoubtedly necessary to pay much attention to avoid further degradation of the existing cropland. Recently, several new conservation agriculture models, including sugarcane (SG), mulberry (MB) and forage grass (FG) cultivation, have been developed as new raising models to replace MS in the karst region6,29. Previous studies have reported that bulk soil OC change under cropland use change in karst region6,7, little is known about soil aggregate fractions and their contribution to bulk soil OC accumulation following cropland conversion. In the karst region, macro-aggregates generally prefer higher soil OC content than micro-aggregates following vegetation or cropland restoration8,30. Further research demonstrated that macro-aggregate contained much more OC stock than other size fractions30,31. Rationally, we hypothesized that the accumulation pattern of bulk soil OC was closely related to the changes of OC stock associated with macro-aggregates. The OC stocks within aggregates were predominantly determined by the amount of aggregates and its associated OC content19,20. Thus, the objective of this study was (1) to determine the distribution and stability of aggregates following cropland conversion, (2) to evaluate the OC content and stocks associated with aggregates following cropland conversion, and (3) to determine whether the changes in OC stocks aggregate-associated were primarily due to changes in the mass of aggregate fractions or to the changes in the OC content associated-aggregate.
Materials and methods
Site description
The study sites were located in Huanjiang County (24°44′–25°33′N, 107°51′–108°43′E) of Guangxi Zhuang Autonomous Region, southwest China. The detailed climate information was presented elsewhere6,7,29. The karst areas are characterized by gentle valleys embraced by hills and the soil is calcareous leptosols (limestone soil) based on the Food and Agriculture Union Organization World Reference Base for Soil Resources (International Union of Soil Sciences Working Group WRB, 2006)7.
The experiment adopted a randomized complete block design with four blocks (about 9 km2). With each block, one plot (greater than 400 m2 each) was selected for the fields of MS, MB, SG and FG. It is noted that the latter three types were converted from MS cultivation and had been under the same crop management for 15–20 years. Cropland uses were managed under regional typical practices. All of the information, i.e., crop species, tillage frequency, harvest frequency, fertilization rate and replanting frequency, have been described in previous studies6,29. In addition, the planted species in the forage grass field was hybrid Napier grass (Pennisetum purpureum Schumach × P. amreicanum (L.) Leeke). This grass is commonly considered as a perennial fodder crop because of its fast growth rate, drought tolerance, high productivity and protein content32,33. All plots were made available over the valley or bottom slopes. In total, 16 plots (four plots × four cropland use types) were included.
Soil sampling and analysis
Soil samplings were conducted in October 2019. In each plot, we collected four soil profiles (0–30 cm), randomly using soil cores with possible litters removed. Each profile was divided into three horizons, that is, 0–10 cm, 10–20 cm and 20–30 cm. In addition, two pits of 60 × 60 × 30 cm were dug to collect samples using metal rings (volume:100 cm3, SYK-01, China) for the measurement of bulk density at the three horizons. After picked out the possible stones and roots. Soil samples were air-dried, gently crushed and divided into two portions for analysis, including (1) one was passed through 2 mm sieve for determining OC content of bulk soil and (2) aggregation fractions and their OC contents.
Aggregate fractionation
The soil aggregate fractions were determined by dry sieving methods, as described by Gartzia-Bengoetxea et al. (2009)34. Briefly, 100 g of air-dried soil samples were taken on a nest of sieves (2 mm, 1 mm, 0.5 mm and 0.25 mm), then agitated for 90 s with a sieve shaker at 600 oscillation min−1. The soil retained on each sieve and in the bottom container was collected under 0.25 mm sieve. The soil aggregates were separated as macro-aggregates (> 2 mm), meso-aggregates (2–0.25 mm), and micro-aggregates (< 0.25 mm). To determine the mass distribution of soil fractions, the sub-sample of soil fractions were oven dried at 105 ℃ and weighted at room temperature. A special aggregate sample and bulk soil was ground to pass through a 100 mesh sieve to measure soil OC content by wet oxidation with dichromate redox colorimetric method35. Mean weight diameter (MWD) and geometric mean diameter (GMD) of soil aggregates were calculated as by the following Eq. 23:
where n is the number of aggregates ranges, Xi is the mean diameter of each size fraction, and Wi is the proportion of each size to the total sample (%).
The stocks of OC (Mg C hm−2) in bulk soil were calculated using the following equation:
where D is the thickness (cm) of the soil layer, BD is the bulk density (g cm−3) and OC is the content (g kg−1).
The stocks of organic carbon in aggregates fractions were calculated using the following equation:
where Mi is the amount of soil in the ith size fraction (kg m−2) and OCi is the OC content of the ith size fraction (g kg−1). and Wi is the proportion of the total soil in the ith size fraction (%). Additionally, we applied the procedure recommended by Zhong et al. (2019)19 and Wei et al. (2013)20 to evaluate the relative contribution of changes in aggregate mass and aggregated-associated OC contents to the total changes in OC stocks within each aggregate fraction. We assumed that changes in OC stock within any particular aggregate fraction were induced both changes in OC content of aggregate fraction (F1) and by changes in the mass of aggregate fraction (F2). Hence, the F1 and F2 were calculated using the following equations:
where F1 is the change in OC stock (Mg C hm−2) aggregate associated due to changes in OC content associated with aggregate, F2 is the change in OC stock within an aggregate fraction due to changes in the mass of aggregate fraction, and M is the initial mass of the aggregate fraction in MS, ΔM is the change in the mass of a particular fraction after cropland conversion, C is the final OC content of the aggregate fraction (g kg−1) after conversion, and ΔC is the change in the OC content of the aggregate fraction (g kg−1) caused by cropland conversion.
Statistical data analysis
A two-way analysis of variance (ANOVA) was conducted using SPSS 21.0 (SPSS, Inc., Chicago, IL, USA) to test the effects of cropland use types and soil depth on (i) OC content and stock in bulk soil, (ii) aggregate fraction distribution, and (iii) OC content and stocks associated with aggregate. Person’s correlation analysis was performed to identify the relationships between OC stocks in bulk soil and the proportion of aggregate and its OC content and stock. The difference was considered significant if the p value was less than 0.05.
Ethics approval and consent to participate
All the crop plants in this study were obtained from the local economical crop. Corn, soybean, sugarcane, mulberry and forage grass were not wild and endangered plants, and all the crop experiments were in compliance with relevant institutional, national and international guidelines and legislation.
Results
OC content and stocks in bulk soil
According to two-way ANOVA, cropland use type and soil depth had significant influence on OC content in bulk soil, respectively (Table 1, p < 0.01). The highest value of OC content and stock in bulk soil was found in FG regardless soil depth, while that in SG and MB was not significantly differed from that in MS (Fig. 1, 2). Specially, compared to MS, OC content in FG increased by 51.87%, 47.98% and 35.48%, OC stock increased by 44.80%, 53.91% and 30.54%, at 0–10 cm, 10–20 cm and 20–30 cm, respectively (Figs. 1, 2). Further analysis indicated that OC content and stock in bulk soil was significantly decreased with soil depth under four cropland use types (Table 1, S1).
Aggregate distribution and its stability
Among the five aggregate fractions, macro-aggregates (> 2 mm) dominated the majority portion (77.14 ± 1.4%, averagely) of total soil, whereas micro-aggregates (< 0.25 mm) accounted the lowest proportion (4.6 ± 0.52%, averagely) (Table 2). Cropland conversion significantly impacted the amount of meso-aggregates (0.5–0.25 mm) and micro-aggregates (< 0.25 mm), which was significantly higher in MB, followed by SG, and lower in MS and FG (Table 1, 2, p < 0.05). Furthermore, the proportion of macro-aggregates was significantly increased while that of meso-aggregates (0.5–0.25 mm) and micro-aggregates decreased significantly when MS converted to FG overall (Table 2, p < 0.05), and no significant differences in the proportion of above mentioned fractions were detected in SG and MB fields (Table 2, p > 0.05).
The value of MWD was significantly affected by cropland use type (Table 1, p < 0.01). The MWD value was significantly decreased following the conversion from MS to MB, and there was no significant difference among MS, SG and FG, especially at 10–20 cm soil layer (Fig. 3). The GMD value was not impacted by cropland use type and soil depth, which varied from 0.72 to 1.73 under four cropland types across 0–30 cm soil layers (Fig. 3).
OC content and stocks associated with aggregate fractions
Both cropland use type and soil depth had significant influence on the OC content associated with aggregates (Table1, p < 0.05). However, these indexes were not affected by the interactions between cropland use type and soil depth (Table 1, p > 0.05). OC content within five size fractions were significantly declined with soil depth (Table 1, S1, p < 0.05). Cropland conversion had a significant effect on OC content associated-aggregate at 0–10 cm and 10–20 cm, which were significantly higher in FG fields compared to the other three cropland use types (Fig. 1a–b, p < 0.05). For detail, After cropland conversion from MS to FG, OC content of > 2 mm, 2–1 mm, 1–0.5 mm, 0.5–0.25 mm and < 0.25 mm size fractions increased by 47.08%, 40.18%, 47.33%, 39.33% and 30.66%, respectively (Fig. 1d). Nevertheless, soil OC content associated-aggregate remained consistent at 20–30 cm soil layer across four cropland use types (Fig. 1c, p > 0.05).
The OC stock with macro-, meso- and micro-aggregates fractions, like OC content, showed a clear decreasing trends with soil depth (Table S1, p < 0.05). Whereas, cropland use change only significantly affected the OC stock with macro-, 2–1 mm and micro-aggregate fractions (Table 1, p < 0.05). For instance, OC stocks associated with macro- and 2–1 mm aggregate fractions were significantly increased when MS converted into FG (Fig. 2d, Table S1, p < 0.05). At 0–10 cm and 10–20 cm, FG cultivation significantly increased the OC stock with macro-aggregates. And at 20–30 cm layer, the higher value of OC stock was found in macro-,2–1 mm aggregate fractions in FG (Fig. 2d, p < 0.05). Furthermore, there was no significant variation in OC stocks associated with the above mentioned aggregate fractions detected in MS, SG and MB soils regardless of soil depth (Fig. 2, p > 0.05).
The increase in OC stocks aggregate-associated was varied different following cropland conversion (Table S2). Compared with MS field, the highest increment of OC stocks within macro-aggregate was observed in FG field (increased by 7.05, 5.51 and 5.10 Mg hm−2 at 0–10 cm, 10–20 cm, and 20–30 cm, respectively) (Table S2). Further analysis indicated that the highest incrementary ratio in OC stock within macro-aggregate was observed in FG field at 0–10 cm soil layer (Table S2).
Contributions of the changes in aggregates dynamics to soil OC sequestration
The percentage of OC stock associated-aggregate varied in different aggregate fractions following cropland conversion (Fig. 4). The majority of OC stock was contained in macro-aggregate (67.2–92.3%), followed by meso-aggregate and micro-aggregate underlying four cropland use types across 0–30 cm soil layer (Fig. 4). Moreover, the proportion of OC stock associated with macro-aggregate was higher in FG than in other three cropland use types (Fig. 4).
The dynamics of contribution of the changes in associated aggregates to OC stock were presented in Table 3. Our results showed that the OC content associated with aggregates and OC stocks associated with macro-aggregate and 2–0.5 mm size class were positively correlated with OC stocks of bulk soil (Fig. 5, p < 0.05). In accordance with the results of linear regression analysis, the accumulation pattern of OC stocks in bulk soil following cropland conversion is mainly due to the changes of OC content associated with macro-aggregates, further indicated that the values of F1 and F2 were greatly higher in macro-aggregates than other four size classes, regardless of depth (Table 3). Moreover, the absolute value of F1 and F2 in FG field were distinctly higher than those in SG or MB field with minimal exception (Table 3).
Discussion
Effects of cropland conversion on OC pool in bulk soil
Cropland restoration identified as an efficient ecological project to promote soil C sequestration in karst erosion areas28,30. The conversion from MS to FG resulted in the total soil OC content and stock across 0–30 cm layers increasing by 46.12% and 43.73% respectively. The result was highly coincident with previous studies observed at 0–10 cm layer, which reported that FG cultivation replaced from MS cultivation could remarkably increase soil OC pool in karst region, Southwest China28. In our study, the lower OC content and stock in MS may be partially attributed to the non-returned crop residues and increased exposure of deep soil OM to oxygen under tillage disturbance, resulting in decreased soil OC accumulation through reducing the input of OM and accelerating OM decomposition28,30,37,38. Nevertheless, the conversion from MS to FG can increase the soil OC pool by increasing inputs from crops. For detail, laregly aboverground crops are harvested and removed from the fields each every year for economic production, there is thus a lack of aboverground OC input. Therefore, the root biomass became the main source of OM inputs, and even slight changes in biomass can substantially alter soil C level39. In the present study, the root biomass in FG field was approximately 6 times that in MS field (110.06 ± 17.24 kg hm−2 averagely) (Table S2). Consequently, the higher root biomass in FG are responsible for the corresponding higher C storage of fine root in FG, which is supported by the fact that higher amount of C were stored in the fine roots of FG field compared with that of MS field (Table S2). In fact, several studies have demonstrated that cultivation of perennial grasses is efficient in stimulating soil OC accumulation owing to its great amount of fine roots and underground biomass33,40. Soil disturbance (such as tillage) is one of the main causes of soil C depletion in agricultural systems, and increased tillage practice can result in greater soil C loss41,42,43. Therefore, the frequent tillage conducted in MS field resulted in lower levels of OC than that in FG field under minimal tillage disturbance.
Impacts of cropland conversion on soil aggregates structure and stability
Soil structure plays an important role in soil environment and quality, which is strongly characterized by soil aggregates and their stability43,44. In our study, soil macro-aggregates dominated the largest portion of total soil while meso-aggregates and micro-aggregates were only accounted for a small portion, indicating that cropland conversion could facilitated the formation of macro-aggregates (Table 2). These findings are in line with other studies, wherein that macro-aggregates occupied the major portion of total soil following farmland or vegetation restoration19,30. Tillage disturbance often disrupts aggregates by bringing subsurface soil to the surface, which can readily promote soil C turnover and hinder macro-aggregate formation45. Conversely, minimal tillage experienced and greater accumulation of root residues resulted in higher C accumulation in the FG field. Furthermore, fine roots improved the soil aggregate stability via the interaction with mycorrhizal fungi, which produced exudates and binding agents and promoted the formation of soil aggregates46,47. Therefore, higher inputs of root residue in the soil could enhance the capacity of aggregate re-formation. In fact, these can be supported by the higher value of root biomass and its C stock in the FG field. In addition, forage grass cultivation can enhance the formation of large and stable soil aggregates by fine roots and fungal hyphae through the production of exudates and binding agents, such as humic compounds, polymers and roots48,49. Thus, few tillage disturbance and higher inputs of root biomass in FG field resulted in soil aggregation enhanced, especially macro-aggregates.
Soil aggregate stability can also be characterized by the values of MWD and GMD. Higher MWD or GMD values indicate greater aggregate stability due to more agglomerate ability. The value of MWD in the current study varied from 1.36 to 1.96, which was classified as “stable” by LeBissonnais' categorization of aggregate stability50.
Regardless of soil depth, the FG field had the greatest MWD and GMD values, indicating that its soil aggregates were more stable than those of the other three cropland use types. We may thus draw the conclusion that FG cropland conversion can improve the stability of aggregates based on MWD and GMD.
Changes in OC stocks associated –aggregates following cropland conversion
Cropland use change generally affects soil C sequestration through changing OM inputs and decomposition19. Our study revealed that aggregate-associated OC was significantly higher in FG field than in MS field. These increases were mainly attributed to the new C derived from root residues inputs and decreased losses of OC associated-aggregate by C mineralization in FG soil49. Generally, tillage can breakdown large aggregates into small aggregates, and thus decrease the formation of soil macro-aggregates41,42. Thus, the lower OC content and stock associated-aggregate in MS field can be attributed to the OC loss resulting from soil erosion, and OM input reduction with tillage disturbance8,30,45.
In this study, the effects of cropland conversion on OC content associated-aggregate fractions occurred in the top 20 cm soil layers. In the karst region, approximate 57–89% of crop roots are concentrated in the surface soil layer, which directly affects OM inputs from underground root residues51,52. Meanwhile, tillage practices also happened on top 20 cm soil layer6,28,29. As a result, in soils below 20 cm, little or no tillage disturbance and limited OM inputs resulted in fewer or no distinctly changing levels of OC content associated with aggregate following cropland use change.
Cropland use change not only affected the OC stocks in bulk soil, but also affected the OC stocks associated-aggregates (Table 1). The difference of sensitivity of OC associated-aggregate to cropland use change may affect its contribution to bulk soil OC accumulation30,38. In our study, the macro-aggregate fraction was the most important contributor to total OC stock increase, followed by meso-aggregate and micro-aggregate (Fig. 4). This is primarily due to the higher amount and OC content of macro-aggregates. Overall all cropland use types, the OC stock associated with macro-aggregate in FG field was higher than that in other three cropland types regardless of soil depth (Fig. 4). For instance, OC stocks within macro-aggregate accounted for about 85.40%, 77.72% and 97.55% of total soil OC stock at 0–10 cm, 10–20 cm and 20–30 cm, respectively, under the conversion from MS to FG. Thus, the accumulation pattern of bulk soil OC stocks could closely related with changes of OC stocks associated with macro-aggregate under cropland use change.
The physical protection of OC in aggregates is regarded as one of the main mechanisms for soil OC accumulation through diminishing soil OC degradation and preventing its interaction with mineral particles53,54. In the present study, OC stock in bulk soil correlated substantially with the OC content-associated aggregate following cropland conversion (Fig. 5). Further analysised revealed that OC stocks in bulk soil was significantly correlated to OC stock associated with macro-aggregate (R2 = 0.83, p < 0.01), confirming that macro-aggregates are the major contributor to bulk soil OC accumulation where most OC stocks contained (Fig. 5). These may be caused by the binding agents of macro-aggregate, such as fungal hyphae, mycorrhizal hyphae, bacterial cells, and algae, which are highly dependent on soil OC and develop simultaneously with crop growth and build up a visible organic skeleton to enmesh the mineral particles by adsorption to form young macro-aggregates15,55,56. These patterns can proven by the shift in the increment of soil OC stocks following cropland conversion. Our calculation showed that the incrementary ratio of OC stock associated with macro-aggregate was more than 58% when MS replaced by FG (Table S3). However, the increment of OC stock associated with macro-aggregate in SG and MB field was relatively smaller and even shows a declining trend (Table S3). Based on the theory of hierarchical aggregation, OC stock increases with increasing aggregate size because of larger aggregates are composed of small particles plus organic binding agents15,29. Therefore, OC stocks in macro-aggregate were higher than those in other size fractions (Fig. 4). Overall, our findings demonstrate that OC accumulation was mainly due to the contribution of macro-aggregates. In the title of Table S3, the unit "Mg/hm2" changed "Mg/hm2".
Changes of OC stocks within aggregates are mainly attributed to two factors: (1) changes in the mass of special aggregate fractions and (2) changes in the OC content associated-aggregate fractions19,20. Thus, a better understanding of the dynamic of soil OC pool response to cropland use change is needed to character the changes of mass and OC content within special soil aggregates. Based on our calculation, the contribution pattern of aggregate mass and its OC content to the net accumulation of OC stock within aggregates differed substantially among four cropland use types. Specifically, the increase in OC stock within macro-aggregate was primarily attributed to increases in the OC contents of macro-aggregate and decreases in the mass of macro-aggregate fraction when MS converted to FG (Table 3). Prior work in karst area have reported that a relative higher recovery of soil aggregate structure and larger increase in macro-aggregate amount following vegetation restoration, indicating that OC accumulation relies on a well-developed soil structure30. These changes were also supported by the fact that the value of F1was greater than F2 in macro-aggregate when MS converted to FG (Table 3). Nevertheless, slight decreases in both mass and its OC content associated-aggregate fractions result in few or no changes in OC stock of macro-aggregate when MS converted to SG or MB (Table 3). Therefore, we can conclude that MS converted to FG can effectively improve the bulk soil OC stock in the karst region of southwest China. The OC stocks associated with macro-aggregate contribute to the most to increase bulk soil OC accumulation, which depends on the OC content within macro-aggregate following the conversion from MS to FG.
Conclusions
The results of this study indicate that cropland conversion has a significant impact on the aggregate size proportion and OC stock in bulk soil and aggregate fractions. Macro-aggregates (> 2 mm) were predominant in all cropland use types. The conversion from MS into FG, substantially increase the proportion of soil macro-aggregate and its OC content, resulting in OC accumulation in the bulk soil and macro-aggregates. Aggregate stability (MWD and GMD was higher in FG than other three cropland use types, indicating a higher stability and aggregation under FG field. The increase in OC stocks within macro-aggregate was the major contributor for the increase in bulk soil OC stock, which depends on the increase of OC content within macro-aggregate. Overall, in karst region, FG replaced from MS was suggested as an ecological restoration model to enhance soil C sequestration potential, owing to its role in increasing OC stock of aggregation and aggregate stability.
Data availability
The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (Grant Numbers 31901129).
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All authors contributed to the study conception and design. L.W., D.L and K.X. set up and conduct the experiment, H.T., X.X. and C.L. analysis the data, L.W. wrote this manuscript. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Wen, L., Li, D., Xiao, K. et al. Dynamics of aggregate-associated organic carbon after long-term cropland conversion in a karst region, southwest China. Sci Rep 13, 1773 (2023). https://doi.org/10.1038/s41598-022-27244-1
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DOI: https://doi.org/10.1038/s41598-022-27244-1
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