Abstract
The neuropeptide oxytocin (OXT) modulates social behaviors across species and may play a developmental role for these behaviors and their mediating neural pathways. Despite having high, stable levels of OXT receptor (OXTR) ligand binding from birth, endopiriform nucleus (EPN) remains understudied. EPN integrates olfactory and gustatory input and has reciprocal connections with several limbic areas. Because the role of OXTR signaling in EPN is unknown, we sought to provide anatomical and electrophysiological information about OXTR signaling in mouse EPN neurons. Using in situ hybridization, we found that most EPN neurons co-express Oxtr mRNA and the marker for VGLUT1, a marker for glutamatergic cells. Based on high levels of OXTR ligand binding in EPN, we hypothesized that oxytocin application would modulate activity in these cells as measured by whole-cell patch-clamp electrophysiology. Bath application of OXT and an OXTR specific ligand (TGOT) increased the excitability of EPN neurons in wild-type, but not in OXTR-knockout (KO) tissue. These results show an effect of OXT on a mainly VGLUT1+ cell population within EPN. Given the robust, relatively stable OXTR expression in EPN throughout life, OXTR in this multi-sensory and limbic integration area may be important for modulating activity in response to an array of social or other salient stimuli throughout the lifespan and warrants further study.
Similar content being viewed by others
Introduction
Oxytocin (OXT) is a nine amino acid neuropeptide that acts through the oxytocin receptor (OXTR) to generate physiological responses in adult mammals, such as facilitating milk let down for lactation, and labor and delivery. OXT also acts in the central nervous system to modulate social behavior by facilitating social learning and memory1, 2, social recognition3,4,5, social reward6, 7, pair-bond formation8, 9, parental care10,11,12,13, sexual behavior14,15,16,17, and by reducing aggression18, 19. Specifically, activation of OXT producing neurons in the paraventricular nucleus (PVN) of the hypothalamus and subsequent OXT release increases social behavior, while reduced activation of these neurons results in a decrease in social behavior20. The effect of OXT on many areas of the central nervous system has been investigated, including the hippocampus where it modulates learning and memory21,22,23,24,25,26, areas that receive sensory input (i.e. olfactory bulb, anterior olfactory nucleus, primary auditory cortex) where it modulates sensory input based on the social salience of the signal5, 11, 27 and amygdalar areas in order to decrease fear-related behavior28 and facilitate social recognition2, 29, 30.
One brain area that has received little attention that may play a role in the OXT modulation of behavior is the endopiriform nucleus (EPN). The EPN is involved in the integration of olfactory and gustatory input via reciprocal connections with piriform and primary gustatory cortex31, 32. EPN also has reciprocal connections with forebrain areas, including infralimbic cortex and amygdala (medial, basolateral, anterior cortical amygdala and periamygdaloid area) that are important for processing the valence of sensory input33,34,35 and with areas involved in learning and memory such as entorhinal and perirhinal cortices34. Based on computational modeling, the EPN has been hypothesized to act as a gate between unconscious and conscious perception of an odorant36. Previous autoradiographic receptor ligand binding, transgenic reporter, and immunohistochemical studies have shown that, unlike some other areas of the developing mouse brain that display transient oxytocin receptor (OXTR) expression12, 37, 38, OXTR expression in the EPN is dense and remains relatively stable throughout development from postnatal day 0 (P0) to adulthood (reviewed in Fig. 1)37, 39. Despite robust OXTR expression throughout development, the effect of OXT on the activity of EPN neurons has not been investigated.
OXTR is produced by and present in the endopiriform nucleus (EPN) across development in mice. (A) Previously unpublished autoradiographic images selected from prior work by Hammock and Levitt (2013) demonstrate OXTR ligand binding in the EPN from birth in C57BL/6J mice. White arrowheads in A and A′ (4 × zoom) point to the dorsal EPN in the coronal plane at post-natal day 0 (P0), P7, P14, P21, P35, and P60. OXTR ligand binding was present in both males and females at all ages examined. (A″) There were no significant differences across age or sex. All ages were significantly different from adult OXTR knock-out (n = 3 per age/sex, relative binding mean ± S.E.M., ***p < 0.001). (B) Sagittal view of Oxtr-EGFP reporter mouse at P21 (enhanced with immunofluorescence for EGFP, counterstained with DAPI) demonstrates the expression of the EGFP reporter throughout the rostro-caudal extent of the EPN. (C) There is no EGFP signal in a transgene negative sample. White arrowheads in B and C show the EPN at P21. Scale bar = 0.5 mm.
To investigate a role for OXT signaling at OXTR in the mouse EPN, we used anatomical and electrophysiological methods. To identify the cell-type that predominately expresses Oxtr mRNA in EPN, we used double-label in situ hybridization (RNAscope). To investigate the effect of OXT on EPN neurons in male and female mice at P21-P28, we used whole-cell electrophysiology. To validate the OXTR-dependent effect, we used an OXTR specific ligand and confirmed the absence of an effect in OXTR KO tissue.
Methods
Animals
All protocols and procedures were performed after approval from the Animal Care and Use Committee at Florida State University in accordance with state and federal guidelines and reported in accordance with ARRIVE guidelines. All mice were bred in the animal facilities at Florida State University. Mice expressing enhanced green fluorescent protein (EGFP+) under the control of the oxytocin receptor regulatory region (OXTR-EGFP) in a mixed FVB/N × Swiss-Webster background were originally obtained from GENSAT (GO185Gsat/Mmucd)40. Mice carrying the Oxtr knockout allele (Oxtrtm1.1Knis)41 were fully backcrossed to C57Bl6j. For OXTR-EGFP experiments, a male OXTR:EGFP transgene positive mouse was paired with an EGFP negative female. To generate Oxtr−/− mice in the context of the OXTR-EGFP reporter, the OXTR-EGFP line was crossed with the Oxtr line to generate EGFP+/Oxtr+/−. These EGFP+/Oxtr+/− mice were then bred with Oxtr+/− to generate offspring that could be EGFP+ and also Oxtr+/+, +/−, or −/−. Breeder cages were checked daily for litters and the first appearance of a litter was indicated as post-natal day 0 (P0). All litters were weaned into male and female specific cages at P21. All animals were housed in the vivarium under a 12/12 light cycle and provided ad libitum food and water.
Receptor autoradiography
Autoradiographic images of OXTR ligand binding were generated in a previously reported study37. Previously unpublished images were selected from that dataset to specifically illustrate the presence of OXTR in the EPN across development. Relative densitometry was performed on archived scans of autoradiographic films in ImageJ. The image was inverted and a free-hand circular tool was used to capture the EPN from 3 sections at the level of the anterior commissure. An age-dependent background correction was also collected from each sample near the corpus collosum/dorsal striatum. Three subjects per sex per age (P0, P7, P14, P21, P35, P60) were available for analysis.
EGFP immunohistochemistry
To better visualize the EGFP reporter to examine reporter expression in the EPN, 40 μm free floating sagittal paraformaldehyde fixed brain sections from transcardially-perfused P21 mice were first washed four times (15 min) in PBS, one time (20 min) in 0.5% sodium borohydride in PBS, 4 times (5 min) in PBS, then incubated overnight at room temperature with Chicken anti-EGFP (1:5000; Abcam ab13970, lot# GR3190550-18) in 1% goat serum and 0.3% Triton X100 in PBS. After 4 (15 min) washes in PBS, tissues were incubated for 2 h at room temperature in Alexa Fluor-488 conjugated donkey anti-chicken (1:500; Jackson ImmunoResearch 703-546-155, lot# 142461) in 1% goat serum and 0.3% Triton X100 in PBS. Sections were washed five times (5 min) in PBS, incubated in 0.1 μg/mL DAPI for 5 min, washed five times (5 min) in PBS then mounted onto gelatin subbed slides, coverslipped with Vectashield (Vectorlabs H-1000) and visualized with a Keyence fluorescence microscope. EGFP transgene positive tissue was compared to EGFP transgene negative tissue.
In situ hybridization
RNAscope in situ hybridization was performed using the RNAscope 2.5 HD kit (Advanced Cell Diagnostics, Inc). The following probes were used: Oxtr mRNA:Oxtr-E4_C2, #411101-C2 (targeting sequence 1198–2221 of NM_001081147.1, GenBank); Gad1: #400951 (targeting sequence 62–3113 of NM_008077.4, GenBank); Gad2: #439371 (targeting sequence 552–1506 of NM_008078.2, GenBank); SLC17a7 (VGLUT1): #416631 (targeting sequence 464–1415 of NM_182993.2, GenBank); SLC17a6 (VGLUT2): #319171 (targeting sequences 1986–2998 of NM_080853.3, GenBank). Tissue was collected from a female C57BL/6J mouse (P28), sectioned at 20 μm and stored at − 80 °C until processing.
Biocytin immunostaining
During initial recordings, biocytin (Biotium 90055, 1 mg/ml) was added to the intracellular fluid to verify recording location. After recording, tissue was immediately placed into 0.4% paraformaldehyde for approximately 24 h at 4 °C, then transferred to phosphate buffered saline (PBS, pH 7.4) until immunostaining. After several PBS washes, tissue sections were incubated overnight in PBS-triton, 3% bovine serum and HiLyteFluor Texas Red-conjugated streptavidin (AnaSpec, Inc. 60671, Lot# 57051-066-078; 1:1000). After several washes in PBS-triton, slices were incubated in DAPI (1:1000) for 10 min, mounted and coverslipped with Vectashield (H-1000).
Acute slice preparation
Male and female mice (P21-P28) were used for all electrophysiology experiments. After decapitation, brains were quickly removed from the skull of un-anaesthetized mice and placed into oxygenated (95% O2, 5% CO2), ice cold sucrose solution containing (in mM): NaCl 83; NaHCO3 26.2; NaH2PO4 1; MgCl2 33; CaCl2 0.5; glucose 22; sucrose 72. EGFP expression was verified at the time of dissection by examining the trigeminal nuclei in the base of the skull with a fluorescent light as these ganglia show high expression of OXTR-EGFP fluorescence42. A block of tissue containing the EPN was dissected out and placed into a vibratome containing the sucrose solution, hemisectioned, and coronal slices (300 µm) containing the EPN were sectioned. Slices were then transferred to a holding chamber and incubated at 33 °C for 30–45 min in oxygenated (95% O2, 5% CO2) artificial cerebrospinal fluid (aCSF) containing (in mM): NaCl 119; NaHCO3 26.2; KCl 2.5; CaCl2 2.5; NaH2PO4 1; MgCl2 1.3; glucose 22. Slices were then allowed to equilibrate to room temperature in the same holding chamber for at least 30 min, then individual sections were transferred to the recording chamber mounted on an Olympus BX51WI upright microscope equipped with brightfield and fluorescent capabilities.
Whole cell electrophysiology
Whole cell current-clamp recordings were obtained from neurons located within the OXTR-EGFP + EPN. The EPN was identified by a dense expression of EGFP identified via excitation with blue light in the OXTR-EGFP mouse line and by using neuroanatomical landmarks described below. Under high magnification on our recording apparatus, there were OXTR-EGFP+ cells and similarly sized cells that did not have EGFP levels detectable by our camera. Healthy cells from both groups were patched under brightfield illumination and showed no significant differences in electrophysiological responses to the experimental conditions so responses were pooled together for these analyses. For whole-cell recordings, glass pipettes (3–8 MΩ, 1B150F-3, World Precision Instruments) were pulled using a horizontal puller (Sutter Instruments, Inc) and had an initial resistance of 3–7 MΩ.
Pipettes were filled with an intracellular fluid that contained (in mM): KMeSO3 145; MgCl2-6 H2O 1; Hepes 10, EGTA 1.1, Na-ATP 2; Na-GTP 0.4 (pH 7.3–7.4, ~ 280 mOsm). Whole-cell configuration was performed only after acquiring a GΩ seal and cells with an initial access resistance higher than 25 MΩ were excluded from analysis. All recordings were performed with a Multiclamp 700B amplifier (Molecular Devices) and controlled by PClamp software (Version 10, Molecular Devices). Capacitive transients were automatically compensated and all data was acquired at 10 kHz. An experimentally defined junction potential of − 11 mV was applied to all traces.
All agonists and antagonists were bath applied prior to, during and after addition of oxytocin or the OXTR specific ligand, threonine4 glycine7 oxytocin (TGOT), in order to obtain accurate baseline, experimental and washout data respectively. The following antagonists were used: GABA receptor antagonist, picrotoxin (100 µM, P1675, Sigma-Aldrich); NMDA glutamate receptor antagonist, APV (10 µM, K3375, Sigma-Aldrich); AMPA/Kainate glutamate receptor antagonist, DNQX (5 µM, #189, Tocris Bioscience); sodium channel blocker, Tetrodotoxin (TTX, 1 µM, Tocris Bioscience, #1078). The oxytocin neuropeptide (200 nM, 06379, Sigma-Aldrich) and the oxytocin-receptor specific agonist, TGOT (200 nM, Bachem, H-7710) were also bath-applied as indicated in the Results section. To avoid any confounding effect of prior exposure to agonist/antagonist bath solutions, only one cell was recorded per slice. Once the tissue was exposed to an experimental bath solution, the tissue was discarded after recordings.
Data quantification
All quantification was performed in Clampfit 10.7. Resting membrane potential was calculated as the average membrane potential over 10 s prior to current injection of every sweep collected during the experiment. Resistance was calculated from the steady state voltage response to − 50 pA current injection. Number of evoked action potentials was in response to 50–150 pA current injection determined for each cell to elicit 3–10 action potentials per sweep under baseline conditions.
Statistical analyses were performed using Graphpad Prism 8. All electrophysiological data was analyzed as a one-way repeated measures ANOVA with Greenhouse–Geisser correction followed by Dunnett multiple comparisons post-hoc test comparing drug application and washout to baseline conditions. Due to the small sample size, we were underpowered to analyze the effect of sex in the statistical analyses. Graphical analysis of the residuals was used to test the statistical assumptions for the ANOVA. Cohen’s d was calculated by dividing the mean difference between the two groups by the pooled standard deviation to determine the effect size. All data are presented as mean +/− SEM. The sample size for statistical analysis is at the level of the individual cell/slice and is indicated along with the number of animals used in the Results section and Supplementary Table 1. Supplementary Table 1 also contains the results for each statistical assay. The data used for each statistical test is included in Supplementary Tables 2–14.
Significance statement
There is a high level of oxytocin receptor (OXTR) expression in the mouse endopiriform nucleus (EPN) throughout development, however little is known about the effect of oxytocin (OXT) on these neurons. We show here that Oxtr mRNA co-expresses mainly with neurons expressing VGLUT1, a glutamatergic cell marker. Using OXTR-EGFP mice to identify EPN, we show that OXT has a mainly excitatory effect on EPN neurons. Thus, activity in EPN neurons may be modulated by OXT during exposure to salient or social stimuli throughout development and this could affect development of behavioral responses during social exposure.
Results
Previously published autoradiography experiments have identified areas with high levels of OXTR ligand binding using the highly selective ligand ([125I]-OVTA) in mice. Unpublished images from this published dataset37 demonstrate OXTR ligand binding in the EPN of C57BL/6J mice from post-natal day 0 (P0) through P60 (Fig. 1A). Two-way ANOVA on densitometry of relative OXTR binding in the EPN across ages and sex did not indicate any significant main effects of sex (F (1, 34) = 0.474, p = 0.498), age (F (5, 30) = 0.392, p = 0.849), or a sex × age interaction (F (5, 24) = 0.729, p = 0.609). However, as expected, Bonferonni-corrected t-tests confirmed that OXTR ligand binding levels for all ages (P0, P7, P14, P21, P35 and P60) were greater than ligand binding from adult OXTR KO tissue (p < 0.001). To attempt to identify potential sources of the high levels of OXTR ligand binding in the EPN, we performed EGFP immunohistochemistry in perfused tissue from the OXTR-EGFP reporter line. Robust EGFP immunoreactivity was evident within the EPN of P21 sagittal sections of OXTR-EGFP reporter mice (Fig. 1B), but not in mice without the EGFP transgene (Fig. 1C). Investigation of the cell types that express OXTR was performed using RNAscope in situ hybridization staining to identify co-expression of Oxtr and several markers for glutamatergic and GABAergic cell types (Fig. 2). Within the EPN, most Oxtr mRNA expressing neurons co-express Slc17a7, a marker for VGLUT1 (61.08%, Fig. 2C,E), a glutamate transporter protein located on the membrane of glutamate-containing vesicles43 that is commonly used to identify glutamatergic neurons and is commonly found in the neocortex, hippocampus and basolateral amygdala44. A low level of Slc17a6 mRNA, a marker for VGLUT2 cells, was also found in the EPN, however, co-localization of Oxtr mRNA expressing cells was much lower than that seen with VGLUT1 (9.71%, Fig. 2D,E). Subpopulations of GABAergic cells can be identified by the presence of two different glutamate decarboxylases, GAD65 and GAD67, enzymes that are responsible for the transition from glutamate to GABA and CO245. The GAD65 and GAD67 proteins are encoded by two distinct genes, Gad1 and Gad2 respectively. Both Gad1 and Gad2 mRNA were found in EPN neurons. Although the density of expression for Gad1 and Gad2 was lower than that seen for VGLUT1, there was still some co-expression of Oxtr-mRNA and both Gad1 (9.61%, Fig. 2A,E) and Gad2 (1.45%, Fig. 2B). A greater percentage of VGlut1 and VGlut2 neurons co-expressed Oxtr mRNA (76.75%, 51.88%, respectively) than Gad1 and Gad2 mRNA expressing neurons (45.25%, 21.59%, respectively) (Fig. 2F). Overall, the results of the mRNA expression show that Oxtr mRNA is densely expressed in the EPN, such that Oxtr mRNA was expressed in almost all cells identified by the hematoxylin counterstain. Further, the majority of these Oxtr mRNA expressing neurons are glutamatergic with a small subpopulation of GABAergic Oxtr-mRNA neurons within the EPN.
RNAscope in situ hybridization indicates that most of the endopiriform nucleus (EPN) cells are VGLUT1 (Slc17a7) positive and ~ 60% of these cells co-express Oxtr, with a small population of GABAergic Oxtr+ cells. Some neurons that express Oxtr mRNA (red) co-express mRNA for the GABAergic markers (black arrows), Gad1 (A, blue) or Gad2 (B, blue). However, most Oxtr expressing cells were negative for these markers (white arrows). Most of the Oxtr expressing neurons in EPN co-express the markers for VGLUT1 (Slc17a7) (C, E, blue, black arrows) with only a few that expressed only Oxtr (C, F, white arrows). Expression of the marker for VGLUT2 (Slc17a6) (D, blue) was low in EPN but some co-expression with Oxtr was detected (black arrows). The percentage of Oxtr expressing EPN neurons that co-express one of the other markers studied here is shown in E, while the percent of EPN neurons that express one of the other markers that co-express Oxtr is shown in F.
To further study the EPN neurons, a transgenic mouse line (OXTR-EGFP) was used to easily identify the OXTR-expressing neurons in live tissue slices. This mouse line expresses a green fluorescent protein under the OXTR-promotor region as seen in Figs. 1B, 3B,C. The EPN is located just lateral to the external capsule and medial to the piriform cortex so these cytoarchitectural landmarks were used to identify the EPN in live tissue slices. The EPN was distinguished from the claustrum by the location of the rhinal fissure with EPN located ventral and claustrum dorsal. During initial electrophysiological recordings, the location of the patch clamped cell was identified by adding biocytin (1 mg/ml) to the intracellular solution, followed by post-hoc immunostaining. In these recordings, the biocytin filled cell (Fig. 3A,C) was located within the EPN as identified by the cytoarchitectural landmarks described above and by OXTR-EGFP expression (Fig. 3B, C). All subsequent electrophysiological recordings were performed using the same parameters, however biocytin was not added to the intracellular solutions to limit any potential effect of biocytin on the health or behavior of the cells during the recordings. We then performed whole-cell patch clamp electrophysiology to investigate the effect of oxytocin and an oxytocin receptor selective agonist, TGOT (threonine4 glycine7 oxytocin) on EPN neurons46. For these experiments, all agonists and antagonists were bath applied at a rate similar to the aCSF drip rate (~ 1 drip/sec). To estimate the time necessary for sufficient fill and turnover of the fluid in the recording chamber in this setup (e.g. dependent on bath size and drug delivery distance), evoked action potential firing was monitored after the start of the tetrodotoxin (TTX) bath. Action potential firing was abolished approximately 3–4 min after starting the bath application, so all experimental data was collected at 4 min and 6 min after the start of bath application to allow for complete turnover of the bath solution and investigate whether the effect of OXTR activation was fast (4 min, similar to TTX effect) or slow/prolonged (6 min). In EPN neurons held just above threshold by injecting positive current in current clamp recordings, there was a significant effect of bath application of oxytocin (200 nM) (One-way repeated measures (RM) ANOVA F (2.112, 10.56) = 6.315, p = 0.015, n = 6 cells, 3 animals-1 male/2 female). Post hoc comparisons using the Dunnett’s multiple comparisons test (MCT) showed that EPN neurons exhibited more spontaneous action potentials at 4 min into OXT bath application compared to baseline levels (M∆ = 32.83, SE 6.779, Q = 4.843, df = 5, p = 0.011, d = 1.87, Fig. 4A, Supplementary Table 1 Line A). The number of spontaneous action potentials fired at 6 min was not significantly different from baseline and the cells returned to baseline levels of spontaneous firing after 20 min of washout with aCSF (M∆ = -6.667, SE = 8.265, Q = 0.807, df = 5, p = 0.768).
OXTR-EGFP expressing neurons in the endopiriform nucleus (EPN). During initial electrophysiological recordings, biocytin was added to the intracellular solution and injected into the recorded cell. Post-labelling of these cells (A) revealed anatomical location of neurons recorded from during electrophysiological experiments. Endogenous expression in the OXTR-EGFP mouse tissue slice (300 µm) shows dense OXTR-EGFP expression in the EPN (B). The combined image (C) shows that the location of the cell recorded from was the OXTR-EGFP rich EPN.
Oxytocin and TGOT have a depolarizing effect on EPN neurons. When held near the action potential threshold in current clamp recordings, bath application of OXT (A, 200 nM) and TGOT (B, 200 nM) elicited a significant increase in the number of spontaneous action potentials at 4 min after the start of application. Representative trace (C) shows increase in the spontaneous action potential firing after start of TGOT (red line) bath application which persists for several minutes. In a separate group of cells at their resting membrane potential, the membrane potential (D) was significantly depolarized with an increase in the number of evoked action potentials (F) at 4 and 6 min after the start of TGOT bath application. A significant increase in membrane resistance was detected only at the 6 min timepoint in this experiment (E). Representative traces from one neuron show the increase in evoked action potential firing at 4 and 6 min with a return to baseline levels during washout (G–J). In a separate experiment using OXTR-KO EGFP mice, TGOT bath application had no significant effect on the cells membrane potential, resistance or number of evoked action potentials (K–M, representative traces in N–P). *p < 0.05; **p < 0.005; ***p < 0.001.
OXT not only binds to the OXTR which we have shown is present in the EPN, but it also binds to vasopressin receptors47,48,49,50,51 so it is possible that the effect seen above was due to OXT binding at vasopressin receptors. To avoid binding at vasopressin receptors, the OXTR selective agonist TGOT was used for the remainder of the experiments46. Using the same experimental design as described above, an aCSF solution containing TGOT (200 nM) was bath applied to the tissue slice during whole-cell patch clamp electrophysiological recording of an EPN neuron held just above threshold in current clamp and the number of spontaneous action potentials was quantified before, during and after TGOT application. One-way RM ANOVA indicated a significant effect of TGOT (F (1.483, 10.38) = 8.918 p = 0.008, n = 8, 2 animals-1 male/1 female) with EPN cells firing a significantly greater number of action potentials at 4 min into the bath application of TGOT (M∆ = 20.13, SE = 6.26, Q = 3.215, df = 7, p = 0.036, d = 1.25) and exhibited significantly fewer spontaneous action potentials than baseline after 20 min of aCSF washout (M∆ = -8.75, SE = 2.22, Q = 3.94, df = 7, p = 0.014, d = 1.35. Figure 4B,C, Supplementary Table 1 Line B).
To further characterize the effect of TGOT on EPN neurons at the resting membrane potential (RMP), a separate experiment was performed at the cells RMP with a three second positive current injection experimentally determined for each cell such that the current evoked between 2 and 10 action potentials during this time (n = 11, 4 animals-2 male/2 female). One-way RM ANOVA indicated a significant effect of TGOT bath application on the EPN cells RMP (F (2.486, 24.86) = 6.165, P = 0.006) with a significantly depolarized RMP at both 4 (Dunnett MCT, M∆ = 2.35 mV, SE = 0.699, Q = 3.362, df = 10, p = 0.019, d = 0.275) and 6 min (Dunnett MCT, M∆ = 3.355 mV, SE = 1.026, Q = 3.271, df = 10, p = 0.022, d = 0.379) after the start of the TGOT bath application compared to baseline, with a return to baseline RMP after the 20 min aCSF washout (i.e. no significant difference between baseline and washout, M∆ = 0.645, SE = 0.737, Q = 0.875, df = 10, p = 0.72) (Fig. 4D, Supplementary Table 1 Line C). TGOT bath application also had a significant effect on EPN cells membrane resistance (F (1.880, 18.80) = 5.533, p = 0.014) with a significant increase in the resistance at 6 min relative to baseline (Dunnett MCT, M∆ = 64.93 MΩ, SE = 12.35, Q = 5.259, df = 10, p = 0.001, d = 0.518) with a return to baseline levels upon washout (M∆ = 14.18 MΩ, SE = 15.16, Q = 0.935, df = 10, p = 0.682) (Fig. 4E; Supplementary Table 1 Line D). A significant effect of TGOT on the number of experimentally evoked action potentials was also found (F (1.713, 17.13) = 5.426, p = 0.018) with significantly more evoked action potentials at both 4 (Dunnett MCT, M∆ = 3.273, SE = 0.715, Q = 4.579, df = 10, p = 0.003, d = 1.185) and 6 min (M∆ = 4.273, SE = 1.129, Q = 3.785, df = 10, p = 0.009, d = 1.739) after the start of the TGOT application with no significant difference between washout and baseline levels (M∆ = 0, SE = 1.543, Q = 0, df = 10, p > 0.999) (Fig. 4F–J; Supplementary Table 1 Line E).
To further confirm that OXTR is necessary for the depolarizing effects of oxytocin and TGOT in the EPN, we crossed the OXTR-EGFP transgene onto an Oxtr−/− line and probed the function of the EPN in response to TGOT (n = 10, 6 animals-4 male/2 female). In the absence of functional OXTRs, there was no significant effect of TGOT bath application on the RMP (F (1.793, 16.13) = 2.628, p = 0.107, Supplementary Table 1 Line F), membrane resistance (F (1.429, 12.86) = 0.194, p = 0.753, Supplementary Table 1 Line G) or the number of evoked action potentials (F (1.552, 13.97) = 0.778, p = 0.447, Supplementary Table 1 Line H) (Fig. 4K–P). These data suggest that TGOT and OXT have a depolarizing effect on EPN neurons in an OXTR dependent manner. However, these data do not show whether this is a direct effect of TGOT/OXT or an indirect effect resulting from the excitation or inhibition of other cells within the tissue slice.
Further experiments were performed under glutamate and GABA receptor blockade to investigate whether the OXTR effects are direct or indirect (n = 13, 5 animals-3 male/2 female). An aCSF solution containing picrotoxin (1 µM) and a combination of DNQX (5 µM) and APV (10 µM) was used to block GABA and glutamate receptors in the tissue slice before, during and after TGOT bath application. Under these conditions, TGOT had a similar effect on the RMP, membrane resistance, and number of evoked action potentials seen previously with only TGOT. Specifically, TGOT bath application had a significant effect on RMP (F (2.070, 24.84) = 6.388, p = 0.005) with significantly higher RMP at 4 (Dunnett MCT, M∆ = 3.379 mV, SE = 1.258, Q = 2.686, df = 12, p = 0.0497, d = 0.444) and 6 min (Dunnett MCT, M∆ = 4.65, SE = 1.466, Q = 3.172, df = 12, p = 0.021, d = 0.606) after the start of TGOT application with no significant difference between baseline and washout RMP (Dunnett MCT, M∆ = 0.089, SE = 1.433, Q = 0.062, df = 12, p = 0.999) (Fig. 5A, D–G; Supplementary Table 1 Line I). A main effect of TGOT on membrane resistance was also identified (F (1.583, 19.00) = 5.443, p = 0.019) with a significantly higher membrane resistance at 4 (Dunnett MCT, M∆ = 45.32 MΩ, SE = 16.37, Q = 2.768, df = 12, p = 0.043, d = 0.243) and 6 (Dunnett MCT, M∆ = 66.34 MΩ, SE = 17.45, Q = 3.801, df = 12, p = 0.007, d = 0.347) minutes into TGOT application with a return to baseline values upon washout (Dunnett MCT, M∆ = 3.091 MΩ, SE = 19.07, Q = 0.162, df = 12, p = 0.997)(Fig. 5B; Supplementary Table 1 Line J). Lastly, TGOT also had a significant effect on the number of evoked action potentials under GABA and glutamate receptor blockade (F (1.610, 19.32) = 4.868, P = 0.025). Specifically, the number of evoked action potentials was higher at both 4 (Dunnett MCT, M∆ = 3.846, SE = 0.815, Q = 4.718, df = 12, p = 0.001, d = 0.53) and 6 min relative to baseline (Dunnett MCT, M∆ = 6.154, SE = 1.213, Q = 5.071, df = 12, p = 0.001) with no significant difference between baseline and washout (Dunnett MCT, M∆ = 1.154, SE = 2.364, Q = 0.488, df = 12, p = 0.928, d = 0.753) (Fig. 5C–G; Supplementary Table 1 Line K). These data show that the effect of TGOT on EPN neurons is not dependent on activation of either GABA or glutamate receptors via local connections from other neurons, suggesting a more direct effect of TGOT on the EPN neurons. However, OXTR has been found on neurons expressing other neurotransmitters, including serotonergic neurons of the raphe nucleus52 and somatostatin containing neurons in the medial prefrontal, visual and auditory cortices11, 53, 54, therefore, other neurotransmitters could be mediating the effect of TGOT and OXT application in these experiments.
TGOT induced depolarization of EPN neurons persists under GABA and glutamate receptor blockade. To limit indirect activity, GABA and glutamate receptors in the tissue slice were blocked during the baseline recording using bath application of picrotoxin (200 nM), DNQX (5 µM) and APV (10 µM) respectively. The addition of TGOT (200 nM) significantly depolarized the membrane potential (A), increased the membrane resistance (B) and the number of evoked action potentials at 4 and 6 min after the start of bath application with a return to baseline levels upon washout in all parameters. Bath application of picrotoxin, DNQX and APV were continued throughout the entire recording. Traces from a representative single EPN cell are shown in (D)–(G). *p < 0.05; **p < 0.005; ***p < 0.001.
To rule out indirect effects from other neurotransmitters, further experiments using tetrodotoxin to block synaptic activity were conducted to test the direct effect of TGOT on EPN OXTR-expressing neurons (n = 19, 8 animals-3 male/5 female). TTX was added to the aCSF and was started ~ 4 min prior to the addition of TGOT. Bath application of TTX was continued throughout the recording (i.e. baseline, TGOT and washout). In the absence of synaptic activity, the depolarizing effect of TGOT persisted, indicating a direct effect of TGOT on the recorded EPN neuron. A significant effect of TGOT bath application on RMP (F (1.795, 32.31) = 19.50, p < 0.0001, RM one-way ANOVA) and membrane resistance (F (1.507, 27.12) = 6.342, p = 0.0095) was found using RM one-way ANOVAs. Dunnett MCT post hoc tests indicated a significant increase in the RMP at both 4 (M∆ = 1.957 mV, SE = 0.518, Q = 3.778, df = 18, p = 0.004, d = 0.404) and 6 min (M∆ = 2.007 mV, SE = 0.393, Q = 5.103, df = 18, p = 0.0002, d = 0.407) after the start of TGOT bath application and a significant hyperpolarization of the membrane potential upon washout (M∆ = −2.343 mV, SE = 0.782, Q = 3.012, df = 18, p = 0.02, d = 0.409) (Fig. 6A,C; Supplementary Table 1 Line L). A significant increase in membrane resistance above baseline levels was also found (Dunnett MCT) at 4 (M∆ = 26.9 MΩ, SE = 8.746, Q = 3.075, df = 18, p = 0.017, d = 0.166) and 6 min (M∆ = 32.99 MΩ, SE = 10.65, Q = 3.098, df = 18, p = 0.017, d = 0.202) post TGOT application with no significant difference between baseline and washout levels (M∆ = -23.07 MΩ, SE = 17.87, Q = 1.291, df = 18, p = 0.447) (Fig. 6B,D; Supplementary Table 1 Line M).
Depolarizing effect of TGOT on EPN neurons persists in the absence of network activity. The direct effect of TGOT on EPN neurons was investigated by using tetrodotoxin (TTX, 1 µM) to block all sodium channel activity. In the absence of network activity, bath application of TGOT depolarized the membrane potential (A) and increased the cells membrane resistance (B) at 4 and 6 min after the start of bath application. A significant hyperpolarization of the membrane potential was detected under these conditions as well (A). Average membrane potential (C) and resistance (D) values collected once every sweep (50 s) during TTX/TGOT protocol were averaged for all 19 cells showing the time course of TGOT-mediated effects, with SEM indicated in gray. *p < 0.05; **p < 0.005; ***p < 0.001.
Discussion
The experiments outlined in this paper were designed to test the hypothesis that OXTRs are expressed on a specific subpopulation of neurons in the mouse EPN and that OXT can modulate the activity of these OXTR-expressing neurons of the EPN. Using RNAscope in situ hybridization, we found a high level of co-localization of Oxtr mRNA and VGlut1 markers in EPN, suggesting that OXTR are highly expressed on VGlut1 expressing neurons within the EPN. To test the effect of OXT on EPN neurons, we used whole-cell patch clamp electrophysiology in a genetic mouse model that expresses EGFP in OXTR-expressing neurons. EPN neurons displayed a depolarizing effect of OXT and the OXTR-specific ligand, TGOT in P21-P28 mice. To ensure the effect of TGOT on EPN neurons was OXTR dependent, we also tested the effect of TGOT on Oxtr KO EPN tissue and found that TGOT did not have a significant effect on EPN cells from Oxtr KO tissue slices.
In the experiments detailed here, a high co-localization of Oxtr and Slc17a7 mRNA, the gene encoding VGLUT1, was found in the EPN neurons, with sparse co-localization of Oxtr and Gad1 and Gad2 mRNA suggesting most Oxtr expressing EPN neurons are glutamatergic. The molecular phenotype of the OXTR-expressing cells determines the indirect effect of OXT on downstream neural targets. For example, in the hippocampus and spinal cord, OXT activation of presynaptic OXTRs facilitates glutamate release from presumably OXTR-expressing glutamatergic neurons55, 56 thus in these examples OXT has an excitatory effect on both the OXTR expressing cell and its downstream target. In other studies, OXT has also been found to modulate cells expressing the inhibitory neurotransmitter, GABA, in several brain regions, including neocortex, piriform, auditory cortex, hippocampus, hypothalamus and olfactory bulb11, 12, 26, 57,58,59. In these examples, OXT can have an inhibitory effect on neurons that receive input from OXTR-expressing, GABAergic cells and could modulate the local excitatory:inhibitory balance. For example, in the auditory cortex, OXT enhances a dam’s pup retrieval in response to calls from a distressed pup and OXT injection into the auditory cortex of virgin dams enhances the auditory signal by balancing inhibitory and excitatory activity, thus making the spike activity more consistent during the call and strengthening the response resulting in behavior similar to an experienced dam11. Similar fine-tuning of action potential firing by OXT has been documented in other brain areas, including hippocampus25, 26, the main olfactory bulb5 and visual cortex53. In these experiments, the depolarizing effect of TGOT in TTX is evidence of a direct effect of OXTR activation on the recorded cell, however these experiments were not designed to investigate the effect of OXTR activation on downstream neural targets and do not rule out a potential network effect of OXT on the excitatory/inhibitory balance in EPN, similar to what has been found in the hippocampus25, 26.
In these experiments, OXT and the OXTR specific agonist TGOT were found to have a depolarizing effect on EPN neurons at both 4 and 6 min after the start of the OXT/TGOT bath application. In a separate study, CA2 hippocampal neurons show a response time of approximately 2 min to TGOT bath application25, however it is not possible to compare the time course of TGOT action in these two studies. In the electrophysiology rig used for these experiments, we showed full blockade of action potential firing via TTX bath application at approximately 3–4 min suggesting that it takes several minutes for full turnover of the bath solution and this turnover time can vary widely depending on the electrophysiology setup. In the experiments described here, the addition of OXT increased the spontaneous firing rate of EPN neurons artificially held near threshold. Since OXT is structurally similar to another neuropeptide, vasopressin, and is able to bind to vasopressin receptors47,48,49,50,51, the OXTR specific agonist, TGOT was used to avoid any confounding effect of vasopressin receptor activation by OXT. TGOT application had a similar effect on EPN neurons in aCSF, again increasing the number of spontaneous action potentials when neurons were held near threshold, and increased the RMP, the number of evoked action potentials and increased the membrane resistance when held at the cells RMP. Under these conditions, it is possible that the effects seen were due to OXT action on neurons presynaptic to the recorded neuron, so glutamate and GABA receptor antagonists were used to block any OXT-mediated increase in pre-synaptic glutamate and GABA release. Similar to aCSF, bath application of TGOT with GABA and glutamate antagonists exhibited increased neuronal excitability on EPN neurons. Other neurotransmitters, specifically somatostatin, are known to co-localize with OXTR in other brain areas, e.g. primary visual cortex53. Based on the possibility of OXT modulating other neurotransmitters presynaptically, a final set of experiments were conducted using TTX to block all network activity. Again, under network blockade TGOT increased the excitability of EPN neurons as indicated by a depolarized membrane potential concurrent with an increase in the membrane resistance. In other brain areas, OXT has been shown to have a depolarizing effect using several different GPCR-mediated pathways. In cultured rat supraoptic neurons, OXTRs work via activation of the Gαq pathway which activates phospholipase C in order to stimulate intracellular calcium release60. Alternatively, activation of the Gq/11 subpopulation of OXTRs is associated with an inhibition of inward rectifying potassium channels which lead to membrane depolarization and a decrease in conductance of potassium across the membrane59, 61,62,63. In this study, OXT and TGOT bath application resulted in a significant increase in the membrane resistance coupled with a significant membrane depolarization in mouse EPN neurons. These results suggest that OXTRs in EPN may be modulating activity via inhibition of a membrane bound channel, possibly through an inward rectifying potassium channel as seen in previous experiments59, 62, 63. Further experiments are necessary to examine whether this is the mechanism by which OXT is affecting membrane excitability in the EPN.
Another experiment was performed here to confirm the depolarizing effect of TGOT on EPN neurons. To retain the ability to use the EGFP reporter to identify EPN neurons in acute slices, the OXTR-EGFP mouse line was crossed with an Oxtr-KO line to get tissue slices in which there are no functional OXTRs, but cells that would normally express these receptors in a wild-type animal express the EGFP marker. The absence of functional OXTRs in this Oxtr-KO line has previously been characterized and these animals exhibit an absence of Oxtr RNA or DNA as seen in Northern and southern blots41, a lack of OXT ligand binding37, 41, 64, and show impairments in social behavior consistent with manipulation of the OXT system41, 65. In these animals, the lack of OXTRs should eliminate any effect of TGOT in EGFP-expressing EPN neurons if the effect is mediated by OXTR activation. As expected, TGOT application had no effect on any of the parameters measured (Fig. 4) in Oxtr KO slices, confirming the OXT-mediated effect on OXTR-EGFP expressing neurons in EPN was due to activation of OXTRs.
For these experiments, we focused on EPN activity in P21-P28 animals in order to characterize the effect of OXTR activation on EPN neurons. At this developmental timepoint, the OXTR expression has already undergone a significant developmental shift in its expression pattern in other brain areas. OXTR expression in neocortex increases from P0 to peak expression at P14 and decreases by P21, although expression in EPN remains relatively stable throughout these timepoints37. We chose to investigate animals at P21 because OXTR expression in neocortex appears to have stabilized by this point, however given the changes in neocortical OXTR expression, it is possible that the EPN response to OXTR activation may change over the course of development. There are also sex differences in oxytocin mediated behaviors and OXTR localization in some brain areas66, however this was not the focus of these experiments and we were underpowered to analyze quantitative sex differences, although qualitative differences were not observed as neurons from both sexes were depolarized by OXT or TGOT application. Given the differences in OXTR expression throughout development and sex differences in OXT mediated behavior, it will be important to investigate these properties in the future as we gain more insight on the role of EPN and OXT mediated activity and social behaviors.
Although little is known about the role of oxytocin in EPN, it may play an important role in the development of social behavior through learning and memory throughout development. Interestingly, OXT has been shown to contribute to both long term potentiation and long term depression of neuronal activity. In several areas of the brain, including the lateral amygdala67, dentate gyrus68, and dorsal horn nociceptive neurons69, OXT elicits long-term depression. However, in other brain areas like the accessory olfactory bulb and hippocampus, OXT facilitates learning and memory70. OXT also enhances spatial memory in mouse dams24 and can block and rescue stress induced deficits in synaptic plasticity71, 72. In mice, early maternal separation for three hours a day (PND 1–21) produces social behavior impairments and deficits in learning and memory and the production of hippocampal LTP, which can be rescued by giving intranasal OXT for 10 days following maternal separation (PND 22–34)73. Oxytocin receptors in the hippocampus are also necessary for long-term social recognition in mice as deletion of CA2/CA3 OXTR impairs long-term social-recognition memory and blocks the induction of hippocampal LTP22. Based on the role of OXT in LTP and learning and memory in other brain areas, it is possible that OXTRs in EPN are contributing to learning and memory of social information within EPN that occurs throughout development, although future experiments are necessary to investigate this possibility.
The EPN receives input from several areas involved in processing the emotional salience of incoming sensory information, including amygdalar areas (anterior cortical amygdala, periamygdaloid complex and medial amygdala) and the infralimbic cortex34, 35. EPN also receives input from olfactory and gustatory cortices and this information is integrated within the EPN31, 32. EPN also has reciprocal connections with areas involved with learning and memory, specifically the entorhinal cortex which is involved with spatial memory and the perirhinal cortex which receives input regarding sensory information34. Given the reciprocal connections with the areas that process incoming sensory information, emotional salience and memory, EPN is a prime candidate to act as an association area, playing a role in emotional learning of chemosensory information31, 34. The input of OXT via the abundant and stable OXTR expression through development may permit the OXT system to facilitate learning and memory of emotionally salient sensory stimuli during development, thus modifying the development of adult social behaviors. For example, in the presence of OXT, incoming sensory information from the olfactory system may be more likely to induce long-term potentiation and memory formation within the EPN neurons because of the depolarizing effect of OXT.
The OXTR-mediated depolarizing effect of OXT in EPN neurons has been demonstrated here, however because little is known about the function of the EPN it is still unclear how this OXTR mediated effect can modulate behavior. It is possible that the EPN is important for synaptic plasticity and learning of social behaviors throughout development. For example, in situations where OXT is released, i.e. suckling, dam-infant caregiving, social interactions in juvenile and adult mice, OXT action in the EPN may be modulating synaptic connections or downstream excitatory/inhibitory balance to elicit long-term changes in the circuitry. The data here show that OXTR activation from P21-P28 is excitatory to EPN neurons, many of which co-express Oxtr and mRNA markers for VGLUT1. Evidence suggests that EPN is important for integrating sensory information from the olfactory and gustatory system31, 32 and with the data presented here, it may also be integrating OXT signaling which could modulate the salience of incoming sensory information and pass this information on to downstream limbic areas like the amygdala in order to modulate social behavior. Additional experiments are necessary to determine the full extent of OXT-mediated activity in EPN and its downstream neuronal targets, and impact on behavior.
Data availability
All data generated or analyzed during this study are included in this published article, its Supplementary Information files and in the GenBank genetic sequence database (NM_001081147.1, NM_008077.4, NM_008078.2, NM_182993.2, NM_080853.3).
References
Choe, H. K. et al. Oxytocin mediates entrainment of sensory stimuli to social cues of opposing valence. Neuron 87, 152–163 (2015).
Ferguson, J. N., Aldag, J. M., Insel, T. R. & Young, L. J. Oxytocin in the medial amygdala is essential for social recognition in the mouse. J. Neurosci. 21, 8278–8285 (2001).
Bielsky, I. F. & Young, L. J. Oxytocin, vasopressin, and social recognition in mammals. Peptides 25, 1565–1574 (2004).
Ferguson, J. N. et al. Social amnesia in mice lacking the oxytocin gene. Nat. Genet. 25, 284–288 (2000).
Oettl, L.-L. et al. Oxytocin enhances social recognition by modulating cortical control of early olfactory processing. Neuron 90, 609–621 (2016).
Dölen, G., Darvishzadeh, A., Huang, K. W. & Malenka, R. C. Social reward requires coordinated activity of nucleus accumbens oxytocin and serotonin. Nature 501, 179–184 (2013).
Young, K. A., Liu, Y., Gobrogge, K. L., Wang, H. & Wang, Z. Oxytocin Reverses Amphetamine-Induced Deficits in Social Bonding: Evidence for an Interaction with Nucleus Accumbens Dopamine. J. Neurosci. 34, 8499–8506 (2014).
Wang, Z. & Aragona, B. J. Neurochemical regulation of pair bonding in male prairie voles. Physiol. Behav. 83, 319–328 (2004).
Young, L. J. & Wang, Z. The neurobiology of pair bonding. Nat. Neurosci. 7, 1048–1054 (2004).
Bales, K. L. & Saltzman, W. Fathering in rodents: Neurobiological substrates and consequences for offspring. Horm. Behav. 77, 249–259 (2016).
Marlin, B. J., Mitre, M., D’amour, J. A., Chao, M. V. & Froemke, R. C. Oxytocin enables maternal behaviour by balancing cortical inhibition. Nature 520, 499 (2015).
Mitre, M. et al. A distributed network for social cognition enriched for oxytocin receptors. J. Neurosci. 36, 2517–2535 (2016).
Scott, N., Prigge, M., Yizhar, O. & Kimchi, T. A sexually dimorphic hypothalamic circuit controls maternal care and oxytocin secretion. Nature 525, 519–522 (2015).
Bitran, D. & Hull, E. M. Pharmacological analysis of male rat sexual behavior. Neurosci. Biobehav. Rev. 11, 365–389 (1987).
Caquineau, C. et al. Effects of alpha-melanocyte-stimulating hormone on magnocellular oxytocin neurones and their activation at intromission in male rats. J. Neuroendocrinol. 18, 685–691 (2006).
Ludwig, M. & Leng, G. Dendritic peptide release and peptide-dependent behaviours. Nat. Rev. Neurosci. 7, 126–136 (2006).
Yanagimoto, M., Honda, K., Goto, Y. & Negoro, H. Afferents originating from the dorsal penile nerve excite oxytocin cells in the hypothalamic paraventricular nucleus of the rat. Brain Res. 733, 292–296 (1996).
Calcagnoli, F., de Boer, S. F., Althaus, M., den Boer, J. A. & Koolhaas, J. M. Antiaggressive activity of central oxytocin in male rats. Psychopharmacology 229, 639–651 (2013).
Nakahara, T. S. et al. Peripheral oxytocin injection modulates vomeronasal sensory activity and reduces pup-directed aggression in male mice. Sci. Rep. 10, 19943 (2020).
Resendez, S. L. et al. Social stimuli induce activation of oxytocin neurons within the paraventricular nucleus of the hypothalamus to promote social behavior in male mice. J. Neurosci. 40, 2282–2295 (2020).
Lin, Y.-T., Huang, C.-C. & Hsu, K.-S. Oxytocin promotes long-term potentiation by enhancing epidermal growth factor receptor-mediated local translation of protein kinase Mζ. J. Neurosci. 32, 15476–15488 (2012).
Lin, Y.-T. et al. Conditional deletion of hippocampal CA2/CA3a oxytocin receptors impairs the persistence of long-term social recognition memory in mice. J. Neurosci. 38, 1218–1231 (2018).
Raam, T., McAvoy, K. M., Besnard, A., Veenema, A. H. & Sahay, A. Hippocampal oxytocin receptors are necessary for discrimination of social stimuli. Nat. Commun. 8, 2001 (2017).
Tomizawa, K. et al. Oxytocin improves long-lasting spatial memory during motherhood through MAP kinase cascade. Nat. Neurosci. 6, 384–390 (2003).
Tirko, N. N. et al. Oxytocin transforms firing mode of CA2 hippocampal neurons. Neuron 100, 593-608.e3 (2018).
Owen, S. F. et al. Oxytocin enhances hippocampal spike transmission by modulating fast-spiking interneurons. Nature 500, 458 (2013).
Grinevich, V. & Stoop, R. Interplay between oxytocin and sensory systems in the orchestration of socio-emotional behaviors. Neuron 99, 887–904 (2018).
Knobloch, H. S. et al. Evoked axonal oxytocin release in the central amygdala attenuates fear response. Neuron 73, 553–566 (2012).
Gur, R., Tendler, A. & Wagner, S. Long-term social recognition memory is mediated by oxytocin-dependent synaptic plasticity in the medial amygdala. Biol. Psychiatry 6, 66 (2014).
Yao, S., Bergan, J., Lanjuin, A. & Dulac, C. Oxytocin signaling in the medial amygdala is required for sex discrimination of social cues. eLife 6, e31373 (2017).
Fu, W., Sugai, T., Yoshimura, H. & Onoda, N. Convergence of olfactory and gustatory connections onto the endopiriform nucleus in the rat. Neuroscience 126, 1033–1041 (2004).
Sugai, T., Yamamoto, R., Yoshimura, H. & Kato, N. Multimodal cross-talk of olfactory and gustatory information in the endopiriform nucleus in rats. Chem. Senses 37, 681–688 (2012).
Meis, S. et al. Identification of a neuropeptide S responsive circuitry shaping amygdala activity via the endopiriform nucleus. PLoS ONE 3, e2695 (2008).
Behan, M. & Haberly, L. B. Intrinsic and efferent connections of the endopiriform nucleus in rat. J. Comp. Neurol. 408, 532–548 (1999).
Krettek, J. E. & Price, J. L. A description of the amygdaloid complex in the rat and cat with observations on intra-amygdaloid axonal connections. J. Comp. Neurol. 178, 255–280 (1978).
Traub, R. D. & Whittington, M. A. A hypothesis concerning distinct schemes of olfactory activation evoked by perceived versus nonperceived input. Proc Natl Acad Sci USA 119, e2120093119 (2022).
Hammock, E. A. D. & Levitt, P. Oxytocin receptor ligand binding in embryonic tissue and postnatal brain development of the C57BL/6J mouse. Front. Behav. Neurosci. 7, 195 (2013).
Vaidyanathan, R. & Hammock, E. A. D. Oxytocin receptor dynamics in the brain across development and species. Dev. Neurobiol. 77, 143–157 (2017).
Newmaster, K. T. et al. Quantitative cellular-resolution map of the oxytocin receptor in postnatally developing mouse brains. Nat. Commun. 11, 1885 (2020).
Gong, S. et al. A gene expression atlas of the central nervous system based on bacterial artificial chromosomes. Nature 425, 917–925 (2003).
Takayanagi, Y. et al. Pervasive social deficits, but normal parturition, in oxytocin receptor-deficient mice. PNAS 102, 16096–16101 (2005).
Tabbaa, M. & Hammock, E. A. D. Orally administered oxytocin alters brain activation and behaviors of pre-weaning mice. Horm. Behav. 118, 104613 (2020).
Kaneko, T. & Fujiyama, F. Complementary distribution of vesicular glutamate transporters in the central nervous system. Neurosci. Res. 42, 243–250 (2002).
Fremeau, R. T., Voglmaier, S., Seal, R. P. & Edwards, R. H. VGLUTs define subsets of excitatory neurons and suggest novel roles for glutamate. Trends Neurosci. 27, 98–103 (2004).
Soghomonian, J. J. & Martin, D. L. Two isoforms of glutamate decarboxylase: Why?. Trends Pharmacol. Sci. 19, 500–505 (1998).
Busnelli, M., Bulgheroni, E., Manning, M., Kleinau, G. & Chini, B. Selective and potent agonists and antagonists for investigating the role of mouse oxytocin receptors. J. Pharmacol. Exp. Ther. 346, 318–327 (2013).
Anacker, A. M. J., Christensen, J. D., LaFlamme, E. M., Grunberg, D. M. & Beery, A. K. Septal oxytocin administration impairs peer affiliation via V1a receptors in female meadow voles. Psychoneuroendocrinology 68, 156–162 (2016).
Chini, B., Manning, M. & Guillon, G. Affinity and efficacy of selective agonists and antagonists for vasopressin and oxytocin receptors: an ‘easy guide’ to receptor pharmacology. Prog. Brain Res. 170, 513–517 (2008).
Sala, M. et al. Pharmacologic rescue of impaired cognitive flexibility, social deficits, increased aggression, and seizure susceptibility in oxytocin receptor null mice: A neurobehavioral model of autism. Biol. Psychiatry 69, 875–882 (2011).
Song, Z. et al. Oxytocin induces social communication by activating arginine-vasopressin V1a receptors and not oxytocin receptors. Psychoneuroendocrinology 50, 14–19 (2014).
Song, Z. & Albers, H. E. Cross-talk among oxytocin and arginine-vasopressin receptors: Relevance for basic and clinical studies of the brain and periphery. Front. Neuroendocrinol. https://doi.org/10.1016/j.yfrne.2017.10.004 (2017).
Yoshida, M. et al. Evidence that oxytocin exerts anxiolytic effects via oxytocin receptor expressed in serotonergic neurons in mice. J. Neurosci. 29, 2259–2271 (2009).
Maldonado, P. P. et al. Oxytocin shapes spontaneous activity patterns in the developing visual cortex by activating somatostatin interneurons. Curr. Biol. 31, 322-333.e5 (2021).
Nakajima, M., Görlich, A. & Heintz, N. Oxytocin modulates female sociosexual behavior through a specific class of prefrontal cortical interneurons. Cell 159, 295–305 (2014).
Breton, J.-D. et al. Oxytocin-induced antinociception in the spinal cord is mediated by a subpopulation of glutamatergic neurons in lamina I-II which amplify GABAergic inhibition. Mol. Pain 4, 19 (2008).
Mairesse, J. et al. Activation of presynaptic oxytocin receptors enhances glutamate release in the ventral hippocampus of prenatally restraint stressed rats. Psychoneuroendocrinology 62, 36–46 (2015).
Kruglikov, I. & Rudy, B. Perisomatic GABA release and thalamocortical integration onto neocortical excitatory cells are regulated by neuromodulators. Neuron 58, 911–924 (2008).
Maier, P., Kaiser, M. E., Grinevich, V., Draguhn, A. & Both, M. Differential effects of oxytocin on mouse hippocampal oscillations in vitro. Eur. J. Neurosci. 44, 2885–2898 (2016).
Osako, Y., Otsuka, T., Taniguchi, M., Oka, T. & Kaba, H. Oxytocin enhances presynaptic and postsynaptic glutamatergic transmission between rat olfactory bulb neurones in culture. Neurosci. Lett. 299, 65–68 (2001).
Lambert, R. C., Dayanithi, G., Moos, F. C. & Richard, P. A rise in the intracellular Ca2+ concentration of isolated rat supraoptic cells in response to oxytocin. J. Physiol. 478, 275–287 (1994).
Bakos, J., Srancikova, A., Havranek, T. & Bacova, Z. Molecular mechanisms of oxytocin signaling at the synaptic connection. Neural Plast. 2018, 4864107 (2018).
Gravati, M. et al. Dual modulation of inward rectifier potassium currents in olfactory neuronal cells by promiscuous G protein coupling of the oxytocin receptor. J. Neurochem. 114, 1424–1435 (2010).
York, N. et al. Oxytocin (OXT)-stimulated inhibition of Kir7.1 activity is through PIP2-dependent Ca2+ response of the oxytocin receptor in the retinal pigment epithelium in vitro. Cell. Signal. 37, 93–102 (2017).
Greenwood, M. A. & Hammock, E. A. D. Oxytocin receptor binding sites in the periphery of the neonatal mouse. PLoS ONE 12, e0172904 (2017).
Hattori, T. et al. Impairment of interstrain social recognition during territorial aggressive behavior in oxytocin receptor-null mice. Neurosci. Res. 90, 90–94 (2015).
Caldwell, H. K. Oxytocin and sex differences in behavior. Curr. Opin. Behav. Sci. 23, 13–20 (2018).
Crane, J. W., Holmes, N. M., Fam, J., Westbrook, R. F. & Delaney, A. J. Oxytocin increases inhibitory synaptic transmission and blocks development of long-term potentiation in the lateral amygdala. J. Neurophysiol. 123, 587–599 (2020).
Dubrovsky, B., Harris, J., Gijsbers, K. & Tatarinov, A. Oxytocin induces long-term depression on the rat dentate gyrus: Possible ATPase and ectoprotein kinase mediation. Brain Res. Bull. 58, 141–147 (2002).
DeLaTorre, S. et al. Paraventricular oxytocinergic hypothalamic prevention or interruption of long-term potentiation in dorsal horn nociceptive neurons: electrophysiological and behavioral evidence. Pain 144, 320–328 (2009).
Fang, L.-Y., Quan, R.-D. & Kaba, H. Oxytocin facilitates the induction of long-term potentiation in the accessory olfactory bulb. Neurosci. Lett. 438, 133–137 (2008).
Park, S.-H., Kim, Y.-J., Park, J.-C., Han, J.-S. & Choi, S.-Y. Intranasal oxytocin following uncontrollable stress blocks impairments in hippocampal plasticity and recognition memory in stressed rats. Int. J. Neuropsychopharmacol. 20, 861–866 (2017).
Takahashi, J. et al. Oxytocin reverses Aβ-induced impairment of hippocampal synaptic plasticity in mice. Biochem. Biophys. Res. Commun. 528, 174–178 (2020).
Joushi, S., Esmaeilpour, K., Masoumi-Ardakani, Y., Esmaeili-Mahani, S. & Sheibani, V. Intranasal oxytocin administration facilitates the induction of long-term potentiation and promotes cognitive performance of maternally separated rats. Psychoneuroendocrinology 123, 105044 (2021).
Acknowledgements
The authors would like to acknowledge Kristen Abreu, Esther Rodriguez-Forti, and Natalia Fernandez, as well as the animal care staff at Florida State University.
Funding
NIH MH114994.
Author information
Authors and Affiliations
Contributions
L.M.B. and E.A.D.H. designed research and wrote the paper. L.M.B. also performed research and analyzed data.
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Biggs, L.M., Hammock, E.A.D. Oxytocin via oxytocin receptor excites neurons in the endopiriform nucleus of juvenile mice. Sci Rep 12, 11401 (2022). https://doi.org/10.1038/s41598-022-15390-5
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-022-15390-5
- Springer Nature Limited