Abstract
Understanding animal physiological adaptations for tolerating heat, and the causes of inter-individual variation, is key for predicting climate change impacts on biodiversity. Recently, a novel mechanism for transgenerational heat adaptation was identified in a desert-adapted bird, where parents acoustically signal hot conditions to embryos. Prenatal exposure to “heat-calls” adaptively alters zebra finch development and their thermal preferences in adulthood, suggesting a long-term shift towards a heat-adapted phenotype. However, whether such acoustic experience improves long-term thermoregulatory capacities is unknown. We measured metabolic rate (MR), evaporative water loss (EWL) and body temperature in adults exposed to a stepped profile of progressively higher air temperatures (Ta) between 27 and 44 °C. Remarkably, prenatal acoustic experience affected heat tolerance at adulthood, with heat-call exposed individuals more likely to reach the highest Ta in morning trials. This was despite MR and EWL reaching higher levels at the highest Ta in heat-call individuals, partly driven by a stronger metabolic effect of moderate activity. At lower Ta, however, heat-call exposed individuals had greater relative water economy, as expected. They also better recovered mass lost during morning trials. We therefore provide the first evidence that prenatal acoustic signals have long-term consequences for heat tolerance and physiological adaptation to heat.
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Introduction
Life-history traits, reproductive success and survival are strongly tied to animals’ capacity to regulate their body temperature across a wide range of environmental conditions1,2. Recent evidence demonstrates that heat dissipation capacities in endotherms not only limit their survival under extreme heat3 but also their reproductive output during sustained hot conditions4,5. These effects are expected to be particularly pronounced in diurnal animals, and those inhabiting hot deserts, exposed to extreme heat and solar radiation6. As the severity of climate change—and heatwaves—intensifies7, it is crucial to understand species’ potential to adapt to elevated temperatures. Recent models for desert avian communities predict major population declines under forecasted climate change, through increased risks of lethal dehydration, hyperthermia, decline in body mass, and reproductive failure8,9,10.
Physiological mechanisms for endotherm thermoregulation at high environmental temperatures involve evaporative heat dissipation, primarily via respiratory (e.g. panting) or cutaneous (e.g. sweating in some mammals) pathways11. Establishing how selection can act on organisms’ thermoregulatory capacities requires identifying the sources of variation in relevant traits. Increasing evidence demonstrates that thermoregulatory capacities and strategies vary between species12,13 and populations14,15, and importantly, are repeatable within individuals16,17. Nonetheless, individual thermoregulation capacities are also known to vary in response to short-term thermal acclimation18,19. Although such short-term phenotypic flexibility likely contributes to individual survival, it may, by lowering the strength of selection, reduce opportunities for genetic adaptation under climate change20. By contrast, other forms of plasticity, namely developmental plasticity, may benefit population persistence, by generating additional inter-individual variation in phenotypes21.
Early-life conditions are well known to profoundly affect individual development and traits, and potentially allow adaptive programming of individual phenotypes to particular environments22,23. In ectotherms, beyond the noticeable temperature-dependent sex determination in some reptiles24, developmental temperature is also known to influence individual thermal performance curves. For example, in tropical reef fish, high developmental temperatures (+ 1.5 and 3.0 °C above present-day temperature), but also parental exposure to these high temperatures, improved offspring metabolic performance (e.g. reduced resting metabolic rate, increased factorial aerobic scope) in warmer water25. Comparable studies of endotherms are far fewer and mainly limited to effects at early life-stages26. In birds, exposure to high air temperatures either pre- or postnatally increases young individuals´ capacity to maintain lower body temperature in hot conditions27,28,29, including through improved evaporative cooling efficiency in the rock pigeon (Columba livia)30. Surprisingly, adaptive developmental programming for high temperatures also occurs via prenatal acoustic communication31.
Prenatal acoustic communication occurs in a diverse range of avian taxa32. Whilst its role in hatching synchronisation or incubation solicitation at sub-optimal temperatures has long been known, its function for developmental programming was only recently proposed31,33. In the desert-adapted zebra finch (Taeniopygia guttata), parents emit a special “heat-call”, only at high air temperatures16,31,34. Heat-calls are produced through an enhanced form of panting or “vocal panting”, which increases the heat tolerance of the emitter, at the cost of higher water loss16. Heat-call utterance is initiated at an individual-specific air temperature threshold (≥ 35 °C16), and is higher in late incubation than in other breeding stages or in non-breeding inviduals31,34,35. Remarkably, embryonic exposure to these calls adaptively reduces nestling growth at high temperatures, which increases their reproductive success as adults31. In addition, individual thermal preferences at adulthood shift towards hotter breeding nests31. These findings suggest that prenatal exposure to heat-calls shaped individual thermal physiology towards a heat-adapted phenotype that persisted into adulthood, but this remains to be tested empirically. Furthermore, in yellow-legged gulls (Larus michahellis), prenatal acoustic experience was recently found to affect several physiological traits such as telomere length and basal corticosterone level36,37, which suggests a direct impact of prenatal sounds on physiology33.
Heat-directed phenotypes are observed in individuals acclimated to high temperatures or from hot-climate populations. Such phenotypes show greater tolerance to high air temperature extremes19,38, and more efficient evaporative cooling in hot conditions, characterised by shallower increases in resting metabolic rate and/or evaportive water loss38,39. Such differences are expected to be most detectable in challenging conditions, such as when air temperature exceeds typical body temperature, or at the time of day when evaporative cooling demands peak (e.g. morning40,41). In addition, at mild Ta, when evaporative cooling is not needed, heat-adapted individuals may be more efficient at conserving water, notably through changes in skin ultrastructure that reduce cutaneous water loss18,42. In ectotherms, heat-directed phenotypes induced by early thermal environments persited into adulthood in some studies43,44 but not others45,46. In birds, high or low incubation temperatures affect thermal tolerance and metabolism in early life, and potentially basal metabolic rate in adulthood26,47. However, we are aware of only one study investigating the long-term effects of early thermal environment on heat dissipation. In Japanese quail (Coturnix japonica), the effect of postnatal temperature on bill surface temperature persisted until adulthood, whereas that on bill morphology did not48. Overall, therefore, it is still unclear whether early-life conditions have long-term effects on thermoregulation in the heat. Yet, establishing the persistence of heat-directed phenotypes into adulthood is essential to understand inter-individual variation, and therefore the fitness benefits that developmental programming may confer.
We tested the hypothesis that prenatal exposure to heat-calls induces a heat-directed phenotype in adult zebra finches, with long-lasting effects on heat tolerance and thermoregulation. We quantified metabolic rate (MR), evaporative water loss (EWL) and body temperature (Tb) in 34 male and female wild-derived zebra finches, prenatally exposed to playbacks of either heat-calls (treatment group) or control calls (control group). At adulthood, thermoregulatory responses were measured over a standardised sequence of air temperature (Ta) stages, increasing from 27 °C up to 44 °C, a value approaching the species’ thermal limit49. Evaporative cooling behaviours such as panting in birds and vocal panting (i.e. heat-calling) are more common earlier in the day (at a given environmental temperature)16,34,40,50, which suggests higher evaporative cooling demand in the morning. We thus tested individuals in both mornings and afternoons, expecting larger differences between playback groups in the morning. First, we investigated whether prenatal exposure to heat-calls improved adult heat tolerance, as indicated by a lower probability of showing signs of severe heat-stress prompting early termination of the trial. We then tested whether heat-call exposure reduces overall water loss and maximises body-water replenishment, using variation in body mass during and after trials. Second, because activity levels and associated metabolic heat production often vary among conspecific individuals51, we assessed playback effects on these two traits across Ta stages, for all birds tested. Third, we tested for prenatal playback effects on adult thermoregulation capacity (only in calm birds, as customary52,53), at high (above thermoneutrality), then mild, Ta (within and below the thermoneutral zone54). We predicted that heat-call birds would (i) thermoregulate more efficiently than controls at high Ta, evident as lower Tb and/or greater evaporative cooling capacity (quantified by the ratio of evaporative heat loss to metabolic heat production), and (ii) conserve more water (i.e. lower EWL and greater relative water economy RWE) at mild Ta, when evaporative cooling is not needed.
Results
Heat tolerance and body mass variation
As predicted, in morning trials, prenatal exposure to heat-calls significantly increased individuals’ likelihood of reaching the highest Ta (i.e. Ta = 44 °C) during a standardized heat exposure protocol at adulthood (Linear Mixed Model (LMM): playback × time-of-day: est = − 18.718, se = 9.062, p = 0.039): control birds were four times more likely than treatment birds (i.e. 28% versus 7%) to have the morning trial terminated before reaching Ta = 44 °C, on account of severe agitation or reaching thermal endpoints (Fig. 1a). However, for individuals that reached Ta = 44 °C, the probability of completing the trial (i.e. tolerating Ta = 44 °C for 20 min) did not vary with playback (LMM: est = − 3.054, se = 3.755, p = 0.416, Supplementary Table S1).
Prenatal playback and time-of-day also affected body mass fluctuation during and after the trial. Birds lost 0.57 ± 0.02 g (~ 4.7% of their initial mass, massinit) on average during a trial, mostly through cumulative evaporative water loss, and regained 0.55 ± 0.004 g (~ 99.2% of that loss) during the hour post-trial, with access to ad libitum food and water. Birds lost more mass during morning than afternoon trials (LMM: est = − 0.061, se = 0.029, p = 0.041), but there was no difference between playback groups (LMM: est = − 0.045, se = 0.062, p = 0.475, Supplementary Table S2). However, for mass recovery, and therefore body water replenishment, treatment birds regained significantly more mass than controls, overall (LMM: est = 42.007, se = 17.436, p = 0.020), but particularly in the morning, when most treatment individuals regained more mass (even up to 2 or 3 times more) than they had lost during the trial (LMM: playback × time-of-day: est = 64.761, se = 18.659, p = 0.002, Fig. 1b, Supplementary Table S2). In the afternoon, however, the pattern tended to be opposite, and most heat-call birds (10/14) did not completely regain their initial mass (Fig. 1b).
Variation in activity throughout the trial
Activity remained low (activitystage < 1.5; i.e. sleeping, sitting or stepping once) in the first three Ta stages, but then increased at Ta = 42 °C and 44 °C (Fig. 2), when individuals were approaching the species' thermal limit49. When all birds tested were considered, activitystage increased more steeply across the Ta gradient in treatment than control birds (LMM: playback × Ta est = 0.132, s = 0.056, p = 0.021, Fig. 2a, Supplementary Table S3). However, there was no significant difference between playback groups when each Ta stage was considered separately (activitystage: p > 0.296, Supplementary Table S3). Likewise, for individuals reaching Ta = 44 °C, treatment birds were not more agitated than controls at the end of the Ta = 42 °C stage (activity42-end; LMM: est = 0.184, se = 0.160, p = 0.260, Supplementary Table S4); and, among calm individuals from which we obtained thermoregulatory data, activity (in the 10 min before and during each thermoregulatory measurement [activitymeas]), was not higher in treatment birds, at any of the Ta stages (p > 0.273, Fig. 2b, Supplementary Table S5).
Thermoregulatory responses to heat
In addition to improving heat tolerance, prenatal acoustic experience altered individual thermoregulation above the upper critical limit of thermoneutrality (Fig. 3, Supplementary Table S6). Playback affected how MR and EWL increased with Ta (LMMs : playback × Ta p = 0.002 and p = 0.044 respectively). However, similarly to activity, the slope of increase was steeper in heat-call than control birds (Fig. 3a,b).
When considering specifically the most extreme Ta each individual had reached (i.e. Max Ta = 42 or 44 °C), prenatal playback again affected metabolic rate and water loss, in interaction with activity prior and during physiological measurement (activitymeas). MR and RWE increased with activitymeas (and EHL/MHP correspondingly decreased) in treatment but not control birds, resulting in higher physiological values for treatment birds at a given activity level (Table 1, Fig. 4a,b, Supplementary Tables S7 and 8), even though activitymeas itself did not differ (see above). In addition, playback had no significant effect on evaporative water loss or Tb at the highest Ta (Table 1). There was also no diurnal variation in thermoregulatory values at high Ta (Table 1), but Tb was higher in females than males (Table 1).
Thermoregulatory responses at thermoneutrality and below
At thermoneutrality (i.e. Ta = 35 °C), treatment and control birds did not differ in any thermoregulatory variables (Table 1 and Supplementary Tables S7 and S8). However, below thermoneutrality (i.e. Ta = 27 °C), prenatal playback affected water balance as predicted: consistent with lower water requirements, RWE was significantly higher (and EHL/MHP significantly lower; Supplementary Table S8) in treatment birds (Table 1; Fig. 5), while EWL tended to be lower (p = 0.064, Table 1).
Lastly, in agreement with higher mass loss over the trial in the morning (see above), EWL was higher in the morning than afternoon (significantly at thermoneutrality, and marginally at Ta = 27 °C: p = 0.053), and morning RWE was significantly lower (at thermoneutrality: Table 1). At thermoneutrality, males also had higher EWL, EHL/MHP and lower RWE than females (Table 1 and Supplementary Table S8).
Discussion
This study provides the first experimental evidence that prenatal acoustic experience affects avian thermoregulation at adulthood. In line with our hypothesis that heat-call exposure confers heat tolerance benefits at adulthood, heat-call individuals were more likely to reach Ta = 44 °C, at the time of day when respiratory evaporative cooling is most used (i.e. morning)34,40. This was, however, not achieved through reduced thermoregulation costs. Instead, MR and water loss at high Ta were higher in treatment than control birds, partly due to a stronger effect of activity on MR and evaporative cooling capacity. At low Ta, nonetheless, prenatal heat-call exposure did shift individual adult phenotype towards greater water conservation, as predicted. Whereas mass loss (mainly reflecting cumulative water loss throughout the trial) was not significantly different, treatment birds replenished more of their body water post-trial, with most treatment individuals over-compensating for the mass loss in the morning, but not in the afternoon. Overall, our data demonstrate that prenatal exposure to parental heat-calls has multiple long-term effects on individual phenotypes. While more work is needed to establish the fitness impact under natural conditions, our findings suggest the adaptive benefits of heat-call exposure do not involve minimizing the costs of thermoregulation at high environmental temperature extremes, but instead improve water balance and heat tolerance.
Contrary to the notion of phenotypic plasticity lowering thermoregulation costs in summer-acclimatised and heat-acclimated birds19,38,39, we found that MR increased more steeply with air temperature in heat-call birds, to reach higher levels at Ta = 44 °C. This occurred as both activity and the associated increase in metabolic heat production were higher in treatment than control birds (even though activity levels during thermoregulatory measurements did not differ). Nevertheless, treatment birds performed better at high Ta, as they were more likely to reach Ta = 44 °C in the morning (regardless of activity levels). Heat-call birds were therefore able to sustain higher costs of activity, without compromising heat tolerance, which may be beneficial to maintain foraging and breeding activities in hot weather. These effects on adult heat tolerance are also consistent with the previously demonstrated shift in thermal preferences towards hotter microsites at adulthood, and confirm that heat-calling to embryos may represent a novel mechanism for transgenerational heat adaptation31. More studies are nonetheless needed on the physiological impacts of activity under extreme heat. That this impact may be subject to developmental programming is particularly relevant in species such as the zebra finch, which, to avoid lethal dehydration8,10, must fly under extreme heat to drinking water (often several km away).
As predicted, heat-call exposed individuals conserved more water at mild Ta (Ta = 27 °C), as indicated by a higher water economy and lower EHL/MHP. No comparable differences were observed at or above thermoneutrality, possibly because individuals progressively started panting16, thus increasing the ratio of respiratory to cutaneous water loss17. Interestingly, cutaneous water loss has indeed been found to be sensitive to developmental conditions in other avian species30,55. For instance, acclimation to low humidity in house sparrow nestlings (Passer domesticus indicus) reduced fledglings’ cutaneous water loss by lowering the proportion of free fatty acid in the skin55. Whether prenatal acoustic signals increased water economy through a similar mechanism remains to be explored. A recent review nonetheless showed that developmental programming by prenatal sounds and vibrations is far more widespread across taxa than previously thought, including for physiological traits31,36. Here, we add to this evidence by demonstrating that prenatal acoustic communication affects long-term heat tolerance and water balance. That such inter-individual variation persisted well-into adulthood is important for understanding the strength of selection acting on these traits. Yet, to date, studies on developmental programming for heat tolerance had been restricted to the first few months of life, even in well-studied poultry (reviewed by Nord and Giroud26). To our knowledge, our study thus provides the first evidence for the long-term effects (> 6 months) of prenatal experience on endotherm heat tolerance or water balance.
Thermoregulation varied throughout the day, with EWL, EHL/MHP and mass loss higher, and RWE lower, in the morning than afternoon. This was expected, based on birds’ higher reliance on behaviours to enhance evaporative heat dissipation (e.g. panting and vocal panting), compared to behaviours maximising non-evaporative losses (e.g. wing spreading), earlier in the day, at a given Ta34,40,41,50. Following our prediction, morning was also when differences between treatment and control birds were most pronounced, both in terms of the likelihood of reaching Ta = 44 °C and for mass recovery post-trial. Considering that foraging is often most intense in the morning56, including during reproduction57, improved heat tolerance at this time of day may particularly benefit fitness. This is particularly relevant considering the predicted impact of climate change on reproduction9,58.
Lastly, our data also add to the increasing literature on intra- and interspecific variation in avian thermoregulatory performance in the heat. Even though birds in our experiment remained well below the lethal dehydration threshold for small desert passerines (estimated at ~ 15% of body mass loss8), our values for RWE (< 1, indicating higher water loss than water gain from metabolic water production), support the view that zebra finches need to drink to maintain positive water balance49,59. Patterns of thermal physiology documented in the present study are similar to those reported at high temperatures in zebra finches17,49,54 and other small desert passerines in Australia53,60. These data, together with our finding of a heat tolerance limit close to Ta = 44 °C (even though thermal endpoints were not specifically elicited in our study), add to the evidence that, under the same experimental conditions, Australian arid-zone passerines generally possess lower heat tolerance than their counterparts from North America and southern Africa10,53,61 and non-passerine taxa that employ gular flutter or high rate of cutaneous evaporative heat dissipation11. Lastly, sex differences, with a higher Tb in females at high Ta and lower water loss at thermoneutrality, are particularly interesting. Indeed, the zebra finch lacks strong sexual dimorphism, and individuals were not breeding during the study (and therefore did not differ in immediate parental care activity). Overall, a better understanding of inter- and intraspecific sources of variation in thermal physiology is urgently needed to predict the global impact of climate change10.
In conclusion, we have demonstrated that exposure to a prenatal acoustic signal indicating hot conditions to embryos31,34 has long-term effects on the thermal phenotype of adult zebra finches. We found that prenatal heat-call exposure shifted adult phenotype toward higher water conservation in mild conditions, and improved their ability to sustain hot conditions at the most critical time of day (i.e. morning). Such acoustic experience also altered individuals’ activity and its metabolic impact, rather than minimizing thermoregulatory costs at high Ta. These findings highlight the relevance of the acoustic channel to program offspring for long-term environmental conditions31,33,36, and provide a first line of evidence on the role of developmental programming in generating heat-adapted phenotypes in endotherms. Such inter-individual variation is paramount for rapid adaptation to climate change, particularly in desert environments where animals are already facing extreme conditions10.
Materials and methods
All procedures were approved by Deakin University Animal Ethics Committee (G06-2017), the Animal Ethics Committee of the University of Pretoria (protocol EC048-18) and the Research and Scientific Ethics Committee of the South African National Biodiversity Institute (P18/36). All experiments were performed in accordance with Australian guidelines and regulations for the use of animals in research. This study was conducted in compliance with the ARRIVE guidelines (https://arriveguidelines.org).
Experimental acoustic treatments and housing
Experimental birds were wild-derived zebra finches from an acoustic playback experiment previously presented in Mariette and Buchanan31. At laying (Feb–March 2014), eggs were collected from outdoor aviaries (Deakin University, Geelong, Australia), replaced by dummy eggs and placed in an artificial incubator at 37.5 °C and 60% relative humidity. After nine days, whole clutches were randomly assigned to one of two acoustic playback groups: treatment eggs were exposed to heat-calls (aka “incubation calls”) and controls to adult contact calls (i.e. tet calls), whilst both groups were also exposed to common nest-specific calls (i.e. whine calls) to ensure normal acoustic stimulation. Playbacks had 20 min of heat-calls or tet calls per 1h15, separated by silence and whine calls, and played from 9:30 a.m. to 6:30 p.m.31. To avoid any differences in incubation conditions, eggs and sound cards were swapped daily between incubators. After hatching, nestlings were reared in mixed or single-group broods, in the same outdoor aviaries (see Supplementary Material).
At adulthood (March–April 2018), we tested 34 experimental birds (16 females and 18 males; 15 treatment and 19 control birds) at the end of their fourth summer. From February 2018, birds were moved to indoor cages for acclimation, at least 27 days before experimental trials, at a constant room temperature of 25 °C and day-night cycle of 12 h:12 h, and supplied with ad libitum finch seed mix, grit, cucumber and water. After three days, we implanted a temperature-sensitive passive integrated transponder (PIT) tag (Biomark, Boise ID, USA) subcutaneously into the bird’s flank. Subcutaneous PIT tags reduce the risk of injuries and generally yield Tb values similar to those obtained using intraperitoneally-injected tags in small birds such as the zebra finch62,63.
Experimental heat exposure protocol
All birds were tested twice. Each individual’s second trial occurred on a different day than the first, with an average of 16 days between the two trials, but each bird was tested in the morning for one trial (~ 10:30 a.m.) and in the afternoon (~ 2:50 p.m.) for the other, in random order. On average, trials lasted 125 min (range: 93–151 min). The predicted mean digesta retention time for a 12 g bird is ~ 50 min64. Hence, to ensure birds were post-absorptive, they were fasted (but with ad-libitum water) for two hours before each trial, within auditory and visual contact of conspecifics. Birds were then weighed to measure the initial mass (massinit ± 0.01 g), before being placed individually in the metabolic chamber within a temperature-controlled cabinet. There were no significant difference in massinit between heat-call (12.04 ± 0.18 g) and control individuals (12.03 ± 0.15 g; t (60) = − 0.059, p = 0.953).
During each trial, Ta in the metabolic chamber was gradually increased in a succession of “stages”. Trials started with Ta = 27 °C for 25 min or 45 min (for the first or second trial respectively), then Ta = 35 °C for 30 min (i.e. thermoneutrality54, followed by 20-min stages in succession at Ta = 40, 42 and 44 °C. Temperature transition took 1 (for 2 °C) to 6 min (for 8 °C increments).
To “complete the trial”, individuals had to be able to remain in the chamber for 20 min at Ta = 44 °C. Bird behaviour in the chamber was monitored using two infrared video cameras by an experimenter (AP) blind to playback treatments. The trial was terminated early if the bird showed sustained escape behaviour, or reached a thermal endpoint (e.g., loss of balance or severe hyperthermia with Tb > 45 °C16,52). Immediately after trial termination or completion, birds were taken out of the chamber and exposed to room temperature. They were then weighed (massend), given water on their bill, and transferred to the holding room at 25 °C in an individual cage with ad libitum seeds and water. After one hour, birds were weighed again (mass1h). No bird died during the trials.
Thermoregulatory measurements and data processing
We used an open flow-through respirometry system to measure CO2 production and EWL, following Whitfield et al.52 and as commonly used to assess avian thermoregulation in the heat19,53,60. Dry air was pushed into a 1.5-L plastic metabolic chamber, maintained at low humidity levels (< 0.72 kPa in excurrent air) by regulating the flow rate (range: 1–3.5 L.min-1) with a mass flow controller. Air was subsampled and pulled into H2O (RH-300, Sable Systems) and CO2 analysers (CA-10, Sable Systems). Details of the respirometry system and calibration procedures are in the Supplementary Material.
Following Whitfield et al.52, in Expedata, for each Ta stage, we selected the 1-min window with lowest and least variable CO2 and H2O values, after ≥ 10 min (or ≥ 5 min at Ta = 42–44 °C) of stable Ta. We calculated MR and EWL using equations 9.5 and 9.6 from Lighton65, assuming a respiratory exchange ratio (RER) of 0.71 for fasted individuals66. Using a RER of 0.83 (i.e. metabolism of approximately equal mix of lipids and carbohydrates60 did not change any result. We computed relative water economy (RWE) as the ratio of metabolic water production (MWP; calculated from rates of CO2 production) to EWL2,59; and the evaporative cooling capacity as the ratio of EHL (calculated from EWL) to MHP (approximated by MR, see Supplementary Material)67. Body temperature was recorded every 10 s using a PIT tag reader, and averaged Tb calculated for the 1-min sampling window, accounting for 99% equilibrium time68 (6.9 min and 2 min for flow rates of 1 L min−1 and 3.5 L min−1, respectively).
Bird behaviour was monitored every 30 s and activity scored as: 0 = resting or sleeping, 1 = looking around while sitting mostly still, 2 = moving with no or small displacement by stepping, 3 = displacement usually by hopping, 4 = hopping repeatedly or jumping, 5 = sustained escape behaviour, jumping continuously. At each Ta stage, we averaged the activity (i) over the first 3 min at stable air temperature (activitystage) to test for inter-individual differences in activity levels under standard conditions, and (ii) over the 10 min prior and during measurement windows (activitymeas) to account for current and carry-over effects of activity on metabolism (after equilibrium time68). Importantly, as per52, only data from calm birds were retained in analyses of thermoregulatory variables (i.e. here, activity ≤ 3 during the 1-min measurement, as well as the preceding 10 min).
We calculated mass loss over the trial (i.e. massinit-massend) as a proxy for total water loss (including through defecation, as faeces contain 80% water54) and mass recovery post-trial as the percentage of mass loss regained after 1 h (i.e. [(mass1h − massend)/(massinit − massend)] * 100).
Data analyses
All analyses were performed using R (v3.6.1) in RStudio (v1.1.1335). The total data set corresponded to 67 trials (n = 34 birds). One trial (out of 68) could not be used because the flow rate was set incorrectly. As data were restricted to calm birds, and some trials had to be terminated before reaching Ta = 44 °C, analyses were conducted on data from all 67 trials at Ta = 27, 35 and 40 °C, but 55 trials at Ta = 42 °C and 28 trials at Ta = 44 °C (Fig. 3). As on rare occasions the PIT tag angle or position prevented its detection by the antenna, sample sizes for Tb are n = 66 at Ta = 27 °C and n = 65 at Ta = 35 °C. For every model, predictors were centered and scaled and residuals checked for normality and homoscedasticity.
In all models (apart from segmented analyses), we tested for effects of prenatal playback, massinit, time-of-day (AM or PM), trial number (1st or 2nd trial) and sex as fixed factors, together with the interaction between playback and time-of-day, and with individual ID as a random factor. Non-significant interactions (p < 0.05) were not retained (full models are presented in Supplementary Material Tables S1–S8).
Heat tolerance and body mass variation
The effect of prenatal playback on heat tolerance was assessed using two proxies as response variables: the maximum Ta reached (Max Ta = 42 or 44 °C, n = 67), and whether or not individuals reaching Ta = 44 °C (n = 53) completed the trial (i.e., spent 20 min at Ta = 44 °C). We fitted generalized linear mixed-effects models (GLMMs, glmer function from lme4 R package) with a binomial error distribution and the fixed and random effects described above.
The effects on individual total water loss during the trial and subsequent body water replenishment in the following hour were investigated using two LMMs with predictors as described above and either mass loss (n = 67), or post-trial mass recovery (n = 66, as one individual was not weighed after 1 h), as response variables.
Variation in activity throughout the trial
First, considering all birds, we tested how activity varied as a function of increasing Ta. We defined the inflection point for activitystage, for Ta ≥ 35 °C, using a Davies test and the function segmented from the segmented R package69. We then fitted linear mixed models (LMMs, lmer function from the lme4 R package) above the inflection point, with prenatal playback, massinit, trial number, time-of-day, sex and the interaction between prenatal playback and recorded Ta as fixed effects, and trial nested within individual ID as random effects. Given the interaction was significant (see “Results”), we computed separate regression lines for treatment and control birds. Second, we tested for differences between prenatal playback groups on activity, separately at each Ta stage where thermoregulatory values were investigated: at the max Ta reached, Ta = 35 °C and Ta = 27 °C. We used LMMs, with activitystage (i.e. activity in first 3 min at stable Ta) or activitymeas (i.e. activity in the 10 min before and during metabolic measurements; square root transformed) as a response variable. Analyses on activitystage were performed on all birds (except n = 12 when the stage lasted < 3 min at stable Ta before trial interruption), to test for overall playback effects (i.e. n = 55 at max Ta reached, n = 67 otherwise). Analyses on activitymeas however were restricted to calm birds only (i.e. activity scores ≤ 3), to match thermoregulatory analyses (i.e. n = 32 at max Ta and n = 67 otherwise). We used the same fixed and random factors as described above for all statistical analyses, in addition to Ta (= 42 or 44 °C) for analyses at the max Ta reached only.
To establish if there were any bias in trial termination criteria between playback groups (even though the observer was blind to treatment), we tested for differences in activity level during the last 3 min at Ta = 42 °C for birds reaching Ta = 44 °C, (i.e. activity42-end, n = 53 trials). We fitted a LMM with predictors and random effect as described above. Activity42-end was square root transformed to meet linear model assumptions.
Thermoregulatory responses above thermoneutral zone
To investigate individual overall thermoregulatory response to heat, we first defined the upper limit of thermoneutrality (i.e. increase in MR) and inflection points for other variables (EWL, Tb, RWE, EHL/MHP) for Ta ≥ 35 °C, using a Davies test and segmented function, as described above for activitystage. This was then again followed by LMMs above the respective inflection points, with predictors, random effect and interaction as above.
To examine responses at the most extreme Ta stage reached (i.e. Max Ta = 42 or 44 °C, n = 32 trials with measurements on calm individuals), we fitted LMMs on MR, EWL, Tb, RWE and EHL/MHP, with predictors as described above. We included Max Ta (42 or 44 °C) as an additional fixed factor, and activitymeas as a covariate and in interaction with playback, to account for potential activity effects on thermoregulatory values.
Thermoregulatory response at mild temperatures
We examined the effect of playback on each thermoregulatory value (MR, EWL, Tb, RWE and EHL/MHP) (i) at thermoneutrality (Ta = 35 °C, n = 67 trials) and (ii) at mild Ta (Ta = 27 °C, n = 67 trials) using LMMs, with predictors as described above and activitymeas and its interaction with playback.
Data availability
Datasets used in this manuscript are available from the Mendeley Data Repository: https://data.mendeley.com/datasets/t45rjhtk9w/draft?a=9fa8157f-69fa-4753-a5b2-6ed24a5a28be.
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Acknowledgements
We thank the Deakin University Animal Care staff, in particular Rod Collins and Bruce Newell, for their assistance throughout this experiment. We are grateful to Bill Buttemer for his technical assistance and Kate Buchanan for commenting on the manuscript. We also thank two anonymous reviewers for providing feedbacks.
Funding
This work was supported by the Australian Research Council (DE170100824 and DP180101207 to MMM) and the Holsworth Wildlife Research Endowment. International travel costs for A.E.M. were provided by the National Research Foundation of South Africa (Grant no. 119754).
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M.M.M., A.P. and A.E.M. designed the study. M.M.M. carried out the acoustic playback experiment and A.P. gathered, processed and analysed the experimental heat exposure data. A.E.M. and M.M.M. provided support on the data collection and analyses. A.P. and M.M.M. wrote the paper with inputs from A.E.M.
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Pessato, A., McKechnie, A.E. & Mariette, M.M. A prenatal acoustic signal of heat affects thermoregulation capacities at adulthood in an arid-adapted bird. Sci Rep 12, 5842 (2022). https://doi.org/10.1038/s41598-022-09761-1
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DOI: https://doi.org/10.1038/s41598-022-09761-1
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