Abstract
Declining atmospheric nitrogen (N) deposition, through reduction in the direct input of inorganic N, may result in less inorganic N being leached from soils to freshwaters (dissolved inorganic N = DIN). Declining sulphur deposition, through reducing the ionic strength in soil water, increases the solubility and mobility of organic soil compounds and may result in increased leaching of organically bound N to freshwaters (total organic N = TON). It is unknown to which extent these two independents and opposing trends, i.e. DIN decline versus TON increase, may affect the nutrient balance (load, stoichiometry) of river water draining into coastal zones. By combining long-term atmospheric and riverine monitoring data of the five major Norwegian rivers draining to the Skagerrak coast, we show that over the past 27 years (1990–2017) river water nutrient composition, and specifically N stoichiometry has been steadily shifting from inorganic to organic fractions, with correlations to changes in human pressures (air pollution), but especially climate (precipitation, temperature, discharge). This shift in nutrient quality may have large consequences on the nutrient cycling in both freshwater and coastal ecosystems and illustrates the complex interactions of multiple stressors (here: N deposition, S deposition, and climate change) on aquatic ecosystems.
Similar content being viewed by others
Introduction
From fresh to salty, aquatic systems are strongly affected by human influence on air and land1,2,3. Emission reduction in central Europe has resulted in declining atmospheric nitrogen (N) deposition and consequent declining concentrations of inorganic N (dissolved inorganic N = DIN) in Scandinavian lakes and rivers4,5,6. In parallel, declining sulphur (S) deposition, due to measures to reduce the acidification of freshwaters, has increased the solubility and mobility of organic soil compounds, and resulted in an increased leakage of organically bound N from soils to aquatic ecosystems7,8,9 (total organic N = TON). How these two independent and opposing trends (DIN decline versus TON increase) may affect the nutrient balance (load, stoichiometry) of river water draining into coastal zones has to date not been systematically investigated6.
A darkening of coastal waters has been observed in the North Sea and Skagerrak over the past decades10,11. It is hypothesized that this phenomenon might be related to the increased riverine discharge of freshwater (i.e. reduced salinity), as well as the increased discharge of terrestrial organic matter into coastal zones3,12. As the leaching of organic material into aquatic systems is expected to continue due to the numerous interacting drivers affecting land–water interactions, such as climate change (e.g. increased precipitation, warming)7,12,13, and other human activities (e.g. land use changes, population growth)3,13,14, filling the knowledge gap as to whether nutrient loads, and nutrient stoichiometry have been systematically changing over the past decades is of high urgency to improve our understanding of multiple stressor effects on aquatic ecosystems.
In order to understand how alterations of biogeochemical cycles may affect ecosystems, e.g. via bottom-up effects on food webs, analysing the relative abundance of key elements such as carbon (C), N and phosphorus (P) has proven to be a powerful tool for ecologists (ecological stoichiometry)15,16. Traditionally, the total nutrient content has been used as a predictor for the nutrient and limitation status of aquatic systems (e.g. total N versus total P). However, not all total nutrient constituents may be equally bioavailable17,18,19. Instead, various fractions, such as inorganic N or organic N, may be more accurate proxies for determining the bioavailability of a given nutrient pool17,18,20. Further, especially regarding the total organic matter pool, a majority of studies has to date focused on the distribution, flux and bioavailability of C, and to a less extend on N, but also P17,20. However, given the recent observations that the various mobilized fractions of organic material in boreal ecosystems may be much more bioavailable than previously assumed17,21, and that terrestrial riverine input may strongly affect coastal zones22,23,24, it is timely to investigate the existence of systematic long-term shifts in inorganic to organic N ratios in boreal areas with previously high and now declining S and N deposition22.
Lastly, the long-term fate of the deposited N onto boreal catchments is still unresolved, as to date no strong leaching of inorganic N has been reported across Scandinavia5. However, given the increased runoff of organic material, part of the N stored in the terrestrial catchments may leach into aquatic systems in organic form22,25,26. Thus, while the increased leaching of organic N may largely relate to the increased runoff of organic material in general (i.e. induced by declining acidification, increasing precipitation), this leaching may be further enhanced if the terrestrial systems have been enriched in N (i.e. induced by N deposition)26. We suggest that the total organic carbon (TOC) :TON ratio may be used to indicate whether the terrestrial system has been enriched in N over time, where declining TOC:TON may reflect a decline in soil and litter C:N.
The aim of this study was to assess trends and drivers of riverine nutrient loads and nutrient stoichiometry in areas experiencing declining S and N deposition, as well as climate change (here: increased precipitation, temperature). Combining atmospheric and riverine long-term monitoring data (1990–2017) from the five major Norwegian rivers draining into the Skagerrak coast (Fig. 1) we hypothesize that:
-
(1)
Declines in N and S deposition have resulted in decreasing riverine loads of DIN and increasing loads of TON over the past decades.
-
(2)
(a) DIN:TON ratios have decreased in response to declining DIN and increasing TON loads over the past decades.
(b) The decrease in DIN:TON has been stronger than the decrease in DIN:TP, and especially TN:TP over the past decades.
-
(3)
TOC:TON ratios have been declining in recent years as a result of increased N enrichment of terrestrial systems following decades of N deposition.
Results and discussion
Combining data from atmospheric and riverine monitoring programs in Norway, we found a significant declining trend in S deposition (− 5.04% annually, p < 0.001), and a weaker, but significant declining trend in N deposition since 1980 (− 0.42% annually, p < 0.001) (Table 1, Fig. 2a,b). In parallel, we found a decreasing trend in DIN loads (− 0.70% annually, p < 0.001) and an increasing trend in TON loads (2.11% annually, p < 0.001) in the five studied Norwegian rivers over the past 27 years (1990–2017) (Table1, 2) (Fig. 2c,d). Supporting hypothesis 1, these observed declines in DIN and increases in TON loads to the Skagerrak coast, were directly linked to the declining depositions in N and S, respectively (linear mixed effect model analysis, Table 2; Supplementary, Table S2–S6). Further, the recent changes in climatic variables (increasing temperature and precipitation), as well as increased discharge loads were important additional drivers of both the DIN decline and TON increase (Tables 1, 2). Lastly, also total N, as well as total P loads increased significantly over the studied time period (N: 0.45% annually, p < 0.001; P: 0.70% , p < 0.001), while DIN:TON, DIN:TP and TN:TP molar ratios decreased by 2.59, 1.84, 0.29% annually (all p < 0.001) (Table 1). These findings support hypothesis 2, illustrating that nutrient balances of river waters feeding into coastal zones have been shifting systematically in response to the declining anthropogenic S, and N deposition in Europe over the past decades. Supporting hypothesis 3, we found that while TOC loads in Norwegian rivers have been significantly increasing over the past 27 years (0.92% annually, p < 0.001), the TOC:TON ratio has been declining (− 1.09% annually, p < 0.001) (Fig. 2h). This finding indicates that more organically bound N (i.e. TON) has been processed and leached from boreal soils than carbon in the studied areas. However, further empirical studies are needed to determine the processes involved in the cycling and flux of the deposited N on the river catchments27,28,29, but also how these processes may be altered by multiple stressor interactions26,30,31. In sum, all observed trends could be correlated to human pressures on air (deposition), but especially also to the climate (precipitation, temperature) (Table 2; Supplementary, Table S2–S6).
Air pollution poses a threat to human health, but also the natural environment globally, e.g. by causing acidification and eutrophication of freshwaters32. To reduce and control air pollution and its negative impacts on aquatic systems, international agreements setting national emission targets for pollutants such as S and N have been in action for decades (e.g. 1979: UNECE Convention on Long-Range Transboundary Air Pollution). By illustrating the steady declines in both S and N deposition since the 1980s, our results confirm the success of such international agreements (Fig. 2a,b).
Despite the comparably small decline in N deposition, overall DIN loads, as well as DIN:TP ratios in the studied Norwegian rivers have declined significantly over the past three decades (Fig. 2c,e). This showcases that N emission control is an effective measure for reducing riverine DIN loads and that despite most of the deposited N being taken up by the terrestrial system, reductions in N deposition result in reduced leaching of N from catchments. Our results stand in line with a study from Sweden, showing that lake DIN concentrations, as well as DIN:TP ratios have declined across the country in response to declining N deposition from central Europe4. In their study, the authors could further prove that these changes in nutrient availability had strong effects on primary producers as declining N deposition resulted in a shift from N and P colimitation, towards N limitation alone. It is known that shifts towards N limitation may in many cases promote N-fixing cyanobacteria blooms33, especially when N limitation occurs synchronously with temperature increases34. Cyanobacteria may affect higher trophic levels due to their reduced food quality as well as toxic effects35. It is currently unknown whether declining DIN:TP ratios may systematically promote cyanobacteria blooms in Scandinavian freshwaters or downstream coastal systems, especially since the influence of multiple stressors on determining bloom situations may further complicate the forecasting of such shifts36. However, trends in increasing lake cyanobacteria blooms in southern Sweden have already been observed, indicating potential threats for water quality and lake ecosystem production37.
Synchronous to the DIN and DIN:TP declines, we found significant increases in riverine TON loads to Norwegian coastal ecosystems (Fig. 2d). This finding indicates a strong shift in the N nutrient stoichiometry from inorganic to organic fractions (Fig. 2f). Such a systematic shift has previously not been observed in Scandinavian rivers, but is not surprising given the observed freshwater browning and increased runoff of terrestrial organic material from boreal catchments7,13,14 and the simultaneous declines in DIN runoff due to successful N emission reductions4,5,6. Chemical parameters and especially nutrient stoichiometry may be excellent early warning indicators for net ecosystem changes in aquatic systems38,39,40. However, future research and monitoring programs need to move beyond using simple total nutrient contents, as well as beyond focusing dominantly on carbon compounds for assessing net ecosystem impacts16,17,18,20. Despite their ecological importance from fresh to salty habitats, nutrients (N and P) bound to organic material have to date caught comparably less attention41,42,43,44. Focusing on these compounds may be especially important for boreal freshwaters and coastal systems, where overall organic compounds (C, N, P) are systematically increasing, and additionally more bioavailable than previously thought13,21,43,44,45,46. If only measuring total N, P and TN:TP ratios as indicators of the ecosystem’s trophic state, as well as total C or dissolved organic C (DOC) as indicators for the organic matter runoff or browning, our study would not have succeeded in detecting any of the observed strong trends in riverine loads to the coast.
The TOC:TON ratio showed a decreasing trend over the studied time frame (Fig. 2h, Table 1), despite TOC loads steadily increasing especially since 2005 (Table 1; Supplementary, Fig. S1). This trend indicates that soluble organic matter is being enriched in organically bound N. This, coupled with the observed increasing trend in both TOC and TON deserves increased investigation22,25. As no strong leaching of DIN has been observed after N deposition to boreal catchments47,48,49, policy makers in Norway are currently debating whether to use N fertilization to improve the CO2 capture potential in boreal forests50. However, our study showcases that interactions between air (i.e. deposition), climate, vegetation, soil and water, as well as ongoing climatic changes (i.e. increasing precipitation, temperature, discharge) are complex, and net effects of N fertilization and processing within the catchment may be diverse. As examples, potential consequences of forest harvest may include increased TON leaching, but also changes in ground vegetation composition and the removal of base cations with effects on e.g. soil hydrology47,48,49. In Sweden and Finland, the N fertilization of forests is already common practice in order to increase forest biomass harvest49. However, if part of the added N may leach from boreal soils in organic form into freshwaters and potentially costal zones, this consequence and the involved processes deserve increased attention and may call for rethinking of N fertilization practices and policy.
Lastly, seasonality, regionality and climate may strongly impact timing, magnitude, processing, and quality of terrestrial material being flushed into downstream freshwater and coastal systems22,23,24. These links are also indicated by our results where all response parameters were strongly correlated to catchment temperature, precipitation and discharge (Table 2). For example, seasonality may play an important role explaining the coupling between riverine and downstream coastal nutrient concentrations, especially due to differences in water residence times51,52,53. Additionally, changes in vegetation cover and land use may affect the flux of nutrients through the catchment by affecting production, processing, as well as export of terrestrial material7,29,54. For our five studied catchments draining into the Skagerrak, no temporal data of vegetation cover, nor land use was available for the investigated timespan. Specific potential effects of altered vegetation cover, tree line advance and land use for the overall Skagerrak catchment remains to be tested in future studies.
In the Norwegian Skagerrak coast, as well as the North Sea, there has been an observed change in water clarity over the past decades, a phenomenon referred to as coastal darkening10,11. It is hypothesized that this phenomenon might be related to the increased riverine discharge of freshwater, as well as increased discharge of terrestrial organic matter into coastal zones3,12. The results of this study support this hypothesis, with increasing trends in both TOC and TON loads. However, future research needs to investigate whether long-term changes in coastal inorganic: organic ratios correspond to the large-scale shifts found in this study.
Conclusion
Large changes have occurred in Norwegian rivers over the past decades regarding N loads and stoichiometry, where the organic nitrogen fraction has been steadily increasing. This study showcases (1) how closely air, land, and water systems are connected, (2) that national and international policies on non-aquatic compartments such as air, may have a strong net influence on aquatic ecosystems, (3) that chemical parameters and nutrient ratios may be excellent early warning indicators for net ecosystem changes, and lastly, (4) how important long-term data sets are to test for water quality changes in response to multiple stressors.
Methods
Data sources
We analysed time series data for five major Norwegian rivers and associated catchment data to assess trends in, and potential drivers of nutrient loads and nutrient load ratios (i.e. stoichiometric ratios) (Fig. 1; Supplementary, Table S1). The river data stems from the Norwegian river monitoring program55, including discharge data from the Norwegian Water Resources and Energy Directorate (NVE) and concentration data collected by the Norwegian Institute for Water Research. The supporting data includes deposition data from the Norwegian monitoring program on long-range transboundary air pollution in Norway collected by the Norwegian Institute for Air Research56, temperature and precipitation data from the Norwegian Meteorological Institute57,58 and land use data extracted from the public mapping service website of NVE (nevina.nve.no). The trend analyses was run for the complete time periods where continuous data was available, i.e. from 1971 for temperature and precipitation, from 1980 for S and N deposition, from 1990–2017 for discharge, as well as all nutrient related parameters except total organic carbon (TOC) and TOC related parameters. Here, data was available from 1999 as TOC was not added to the monitoring program before that time point for several of the rivers. Mixed effect model analysis, to investigate the drivers behind the respective trends, was conducted for selected time periods where data was available for all relevant parameters with monthly resolution to ensure a balanced statistical design (i.e. selected years were: 2004, 2009, 2014, sample size = 180).
River data
The rivers selected for this study are the five major rivers in the south eastern part of Norway, and all drain to the Skagerrak coast (Fig. 1). Part of these catchments are among areas most heavily affected by atmospheric deposition in Norway, with a gradient decreasing from south to north (Fig. 2a,b)56. Although within the same region, the catchments show a range in catchment size and land use (Fig. 1; Supplementary, Table S1). Water sampling was conducted close to the river mouth, to be representative for calculating the total load from the catchment. The samples were collected monthly, following standard procedures55. The parameters selected for this study were total N (TN), total P (TP), total organic C (TOC), dissolved inorganic N (DIN = NO3 + NH4), and total organic N (TON = TN − DIN). Most of these parameters have been analysed since the start of the program in 1990, but NH4 was not included until 1992. However, given that the NH4 contribution to DIN and TN was comparably small (9 and 5%, respectively), the analysis was conducted for the whole time period (i.e. DIN in 1990 and 1991 only represents NO3 loads). Thus, any detected trends in DIN or TON loads and load ratios may be considered conservative in terms of the hypotheses, given that the initial DIN and TON loads are slightly lower and higher, respectively, than the actual loads. TOC was not measured in all rivers until 1999, wherefore analysis of TOC-related parameters was restricted to this shorter time period (i.e. data included 1999–2017) (Supplementary, Fig. S1). For load calculations, concentration data was linearly interpolated to daily values and then multiplied by daily discharge to give daily loads, which were subsequently summed to monthly values.
Nutrient ratios were calculated as molar ratios, based on the summarised load values; i.e. for the load ratios to be calculated from comparable loads, samples where certain parameters were missing with respect to the parameters that were generally analysed for the specific year/river were excluded. The ratios DIN:TON, TN:TP, DIN:TP, and TOC:TON were selected for the analysis. The N:P elemental ratios can be used as indicators for biological nutrient limitation. Especially the DIN:TP ratio has been shown to be a robust predictor of e.g. phytoplankton nutrient limitation in boreal lakes and coastal regions18,19 and in other areas with high dissolved organic matter59 compared to TN:TP.
Meteorological and deposition data
Daily air temperature and precipitation was available on a 1 km × 1 km grid covering the whole of Norway. Gridded data was averaged for each catchment using the intersection between the delineated catchment and the grid cells, weighting according to grid areas. Monthly values were calculated by summing or averaging across daily values for precipitation and temperature, respectively. Data for 1990 to 2017 was used in the analysis of meteorological data.
Atmospheric deposition data was provided as five-year average total inorganic N and sea-salt corrected S deposition (wet + dry), on a 0.25° × 0.125° grid56. As for meteorological data, a weighted average deposition per catchment was calculated based on the intersection between the catchment and the deposition grid cells. Data from the following time periods was used: 1978–1982, 1992–1996, 1997–2001, 2002–2006, 2007–2011, and 2012–2016 (1978–1982 only included in figure, i.e. Fig. 2a,b). The middle year for each time range was used in the data analysis (e.g. 2014 for time period 2012–2016).
Data analysis and statistical methods
We analysed N loads, load ratios, discharge as well as meteorological data (N, S deposition, temperature, precipitation) for monotonic time series trends. Specifically, we conducted non-parametric seasonal (i.e. month)-regional (i.e. river) Mann–Kendall (MK) test using the “rkt” package in R60. The seasonal-regional MK test is an extension of the MK test, accounting for systematic, unidirectional seasonality and regionality effects in the time series trends60. Specifically, a monotonic upward or downward trend means that the variable consistently increases or decreases (respectively) through time, without, however, implying a linear trend per se. Trends are presented as the overall Theil–Sen’s slope, as well as the calculated percentage change in the mean quantity (e.g. load quantity) per year (Table 1).
To identify and describe the drivers behind the observed significant time trends in DIN and TON loads, as well as DIN:TON, DIN:TP, and TOC:TON molar ratios we conducted linear mixed effect modelling (LME) following standard protocols for model selection and validation61,62 and using the “nlme” package in R63. Prior to analysis, load and load ratio data were log, and square root transformed, respectively, to ensure normal distribution of the response variables. In step 1 of the LME analysis, the random structure of the model was selected based on prior knowledge of the dependency structure in the data (i.e. month nested in river, note that year was kept as a fixed effect as only three levels). In step 2, the model was fitted and the importance and significance of fixed effects evaluated using the likelihood ratio (ML) test to compare alternative models in a stepwise selection process by using the Bayesian information Criterion (AIC) to inform selection to gain the best explanatory model. Initial selected drivers were N or S deposition (N for DIN related parameters, S for TON related parameters), year, temperature, precipitation and discharge (Table 2). Note that no interaction terms were included to avoid overfitting. Lastly, models were validated based on investigating the normalised residuals (using restricted maximum likelihood estimation (REML)) to identify potential violation of homogeneity, as well as verify normality by using the sjPlot package64 in addition to base packages. For details on model results see Supplementary, Table S2–S6. The graphics of this study (Figs. 1, 2; Supplementary, Fig. S1) were produced using the R package “ggplot2”63. All statistical analyses, as well as graphics were conducted using the freely available software R (R CoreTeam 2020, Version 4.0.2, https://www.R-project.org/).
Data availability
Raw data were collected, and are administered by the Norwegian Water Resources and Energy Directorate (discharge data), the Norwegian Institute for Air Research (deposition data), the Norwegian Meteorological Institute (meteorological data), and the Norwegian Institute for Water Research (water related parameters). Derived data supporting the findings, as well as R code to reproduce figures and analysis of this study are available from the corresponding author upon request.
References
Elser, J. J. et al. Shifts in lake N: P stoichiometry and nutrient limitation driven by atmospheric nitrogen deposition. Science 326, 835–837. https://doi.org/10.1126/science.1176199 (2009).
Cloern, J. E. et al. Human activities and climate variability drive fast-paced change across the world’s estuarine–coastal ecosystems. Glob. Change Biol. 22, 513–529. https://doi.org/10.1111/gcb.13059 (2016).
Deininger, A. & Frigstad, H. Reevaluating the role of organic matter sources for coastal eutrophication, oligotrophication and ecosystem health. Front. Mar. Sci. 6, 210 (2019).
Isles, P. D., Creed, I. F. & Bergström, A. K. Recent synchronous declines in DIN: TP in Swedish lakes. Glob. Biogeochem. Cycle 32, 208–225 (2018).
Vuorenmaa, J. et al. Long-term changes (1990–2015) in the atmospheric deposition and runoff water chemistry of sulphate, inorganic nitrogen and acidity for forested catchments in Europe in relation to changes in emissions and hydrometeorological conditions. Sci. Total Environ. 625, 1129–1145 (2018).
Kaste, Ø., Austnes, K. & de Wit, H. A. Streamwater responses to reduced nitrogen deposition at four small upland catchments in Norway. Ambio https://doi.org/10.1007/s13280-020-01347-3 (2020).
Finstad, A. G. et al. From greening to browning: Catchment vegetation development and reduced S-deposition promote organic carbon load on decadal time scales in Nordic lakes. Sci. Rep. 6, 31944. https://doi.org/10.1038/srep31944 (2016).
Monteith, D. T. et al. Dissolved organic carbon trends resulting from changes in atmospheric deposition chemistry. Nature 450, 537-U539. https://doi.org/10.1038/nature06316 (2007).
Oulehle, F. et al. Soil–solution partitioning of DOC in acid organic soils: results from a UK field acidification and alkalization experiment. Eur. J. Soil Sci. 64, 787–796. https://doi.org/10.1111/ejss.12089 (2013).
Aksnes, D. L. et al. Coastal water darkening and implications for mesopelagic regime shifts in Norwegian fjords. Mar. Ecol. Prog. Ser. 387, 39–49. https://doi.org/10.3354/meps08120 (2009).
Dupont, N. & Aksnes, D. L. Centennial changes in water clarity of the Baltic Sea and the North Sea. Estuar. Coast. Shelf Sci. 131, 282–289 (2013).
Frigstad, H. et al. Long-term trends in carbon, nutrients and stoichiometry in Norwegian coastal waters: Evidence of a regime shift. Prog. Oceanogr. 111, 113–124 (2013).
Solomon, C. T. et al. Ecosystem consequences of changing inputs of terrestrial dissolved organic matter to lakes: Current knowledge and future challenges. Ecosystems 18, 376–389. https://doi.org/10.1007/s10021-015-9848-y (2015).
de Wit, H. A. et al. Current browning of surface waters will be further promoted by wetter climate. Environ. Sci. Technol. Lett. 3, 430–435. https://doi.org/10.1021/acs.estlett.6b00396 (2016).
Sterner, R. W. & Elser, J. J. Ecological Stoichiometry: The Biology of Elements from Molecules to the Biosphere (Princeton University Press, Princeton, 2002).
Maranger, R., Jones, S. E. & Cotner, J. B. Stoichiometry of carbon, nitrogen, and phosphorus through the freshwater pipe. Limnol. Oceanogr. Lett. 3, 89–101. https://doi.org/10.1002/lol2.10080 (2018).
Soares, A. R. et al. New insights on resource stoichiometry: assessing availability of carbon, nitrogen, and phosphorus to bacterioplankton. Biogeosciences 14, 1527–1539 (2017).
Bergström, A. K. The use of TN:TP and DIN:TP ratios as indicators for phytoplankton nutrient limitation in oligotrophic lakes affected by N deposition. Aquat. Sci. 72, 277–281. https://doi.org/10.1007/s00027-010-0132-0 (2010).
Ptacnik, R., Andersen, T. & Tamminen, T. Performance of the redfield ratio and a family of nutrient limitation indicators as thresholds for phytoplankton N vs. P limitation. Ecosystems 13, 1201–1214. https://doi.org/10.1007/s10021-010-9380-z (2010).
Berggren, M., Sponseller, R. A., Soares, A. R. A. & Bergström, A. K. Toward an ecologically meaningful view of resource stoichiometry in DOM-dominated aquatic systems. J. Plankton Res. 37, 489–499. https://doi.org/10.1093/plankt/fbv018 (2015).
Soares, A. R. A., Lapierre, J.-F., Selvam, B. P., Lindström, G. & Berggren, M. Controls on dissolved organic carbon bioreactivity in river systems. Sci. Rep. 9, 14897. https://doi.org/10.1038/s41598-019-50552-y (2019).
Lepistö, A., Kortelainen, P. & Mattsson, T. Increased organic C and N leaching in a northern boreal river basin in Finland. Glob. Biogeochem. Cycle https://doi.org/10.1029/2007gb003175 (2008).
Bianchi, T. S. et al. Centers of organic carbon burial and oxidation at the land-ocean interface. Org. Geochem. 115, 138–155. https://doi.org/10.1016/j.orggeochem.2017.09.008 (2018).
Massicotte, P., Asmala, E., Stedmon, C. & Markager, S. Global distribution of dissolved organic matter along the aquatic continuum: Across rivers, lakes and oceans. Sci. Total Environ. 609, 180–191 (2017).
Neff, J. C., Chapin, F. S. III. & Vitousek, P. M. Breaks in the cycle: dissolved organic nitrogen in terrestrial ecosystems. Front. Ecol. Environ. 1, 205–211 (2003).
Liu, L. L. & Greaver, T. L. A global perspective on belowground carbon dynamics under nitrogen enrichment. Ecol. Lett. 13, 819–828. https://doi.org/10.1111/j.1461-0248.2010.01482.x (2010).
Sponseller, R. A. et al. Nitrogen dynamics in managed boreal forests: Recent advances and future research directions. Ambio 45, S175–S187. https://doi.org/10.1007/s13280-015-0755-4 (2016).
Lucas, R. W. et al. Long-term declines in stream and river inorganic nitrogen (N) export correspond to forest change. Ecol. Appl. 26, 545–556. https://doi.org/10.1890/14-2413.1 (2016).
Schelker, J. et al. Nitrogen export from a boreal stream network following forest harvesting: Seasonal nitrate removal and conservative export of organic forms. Biogeosciences 13, 1–12. https://doi.org/10.5194/bg-13-1-2016 (2016).
Liang, X. Y. et al. Global response patterns of plant photosynthesis to nitrogen addition: A meta-analysis. Glob. Change Biol. 26, 3585–3600. https://doi.org/10.1111/gcb.15071 (2020).
Carter, T. S. et al. Mechanisms of nitrogen deposition effects on temperate forest lichens and trees. Ecosphere 8, e01717. https://doi.org/10.1002/ecs2.1717 (2017).
Galloway, J. N. et al. Nitrogen cycles: Past, present, and future. Biogeochemistry 70, 153–226. https://doi.org/10.1007/s10533-004-0370-0 (2004).
Downing, J. A., Watson, S. B. & McCauley, E. Predicting Cyanobacteria dominance in lakes. Can. J. Fish. Aquat. Sci. 58, 1905–1908. https://doi.org/10.1139/f01-143 (2001).
Paerl, H. W., Gardner, W. S., McCarthy, M. J., Peierls, B. L. & Wilhelm, S. W. Algal blooms: Noteworthy nitrogen. Science 346, 175. https://doi.org/10.1126/science.346.6206.175-a (2014).
Brett, M. T. & Müller-Navarra, D. C. The role of highly unsaturated fatty acids in aquatic food web processes. Freshw. Biol. 38, 483–499. https://doi.org/10.1046/j.1365-2427.1997.00220.x (1997).
Richardson, J. et al. Effects of multiple stressors on cyanobacteria abundance vary with lake type. Glob. Change Biol. 24, 5044–5055. https://doi.org/10.1111/gcb.14396 (2018).
Weyhenmeyer, G. A. Warmer winters: Are planktonic algal populations in Sweden’s largest lakes affected? AMBIO A. J. Hum. Environ. 30, 565–571, 567 (2001).
Hessen, D. O., Elser, J. J., Sterner, R. W. & Urabe, J. Ecological stoichiometry: An elementary approach using basic principles. Limnol. Oceanogr. 58, 2219–2236. https://doi.org/10.4319/lo.2013.58.6.2219 (2013).
Wang, L. et al. Early warning of cyanobacteria blooms outbreak based on stoichiometric analysis and catastrophe theory model. J. Math. Chem. 58, 906–921. https://doi.org/10.1007/s10910-019-01052-x (2020).
Scheffer, M. & van Nes, E. H. Shallow lakes theory revisited: various alternative regimes driven by climate, nutrients, depth and lake size. Hydrobiologia 584, 455–466. https://doi.org/10.1007/s10750-007-0616-7 (2007).
Wiegner, T. N., Seitzinger, S. P., Glibert, P. M. & Bronk, D. A. Bioavailability of dissolved organic nitrogen and carbon from nine rivers in the eastern United States. Aquat. Microb. Ecol. 43, 277–287. https://doi.org/10.3354/ame043277 (2006).
Seitzinger, S. P. & Sanders, R. W. Contribution of dissolved organic nitrogen from rivers to estuarine eutrophication. Mar. Ecol. Prog. Ser. 159, 1–12 (1997).
Thompson, S. K. & Cotner, J. B. Bioavailability of dissolved organic phosphorus in temperate lakes. Front. Environ. Sci. https://doi.org/10.3389/fenvs.2018.00062 (2018).
Nausch, M. & Nausch, G. Dissolved phosphorus in the Baltic Sea—Occurrence and relevance. J. Mar. Syst. 87, 37–46. https://doi.org/10.1016/j.jmarsys.2011.02.022 (2011).
Savchuk, O. P. Large-scale nutrient dynamics in the Baltic Sea, 1970–2010. Front. Mar. Sci. 5, 20. https://doi.org/10.3389/fmars.2018.00095 (2018).
Jani, J. & Toor, G. S. Composition, sources, and bioavailability of nitrogen in a longitudinal gradient from freshwater to estuarine waters. Water Res. 137, 344–354. https://doi.org/10.1016/j.watres.2018.02.042 (2018).
QuinnThomas, R., Canham, C. D., Weathers, K. C. & Goodale, C. L. Increased tree carbon storage in response to nitrogen deposition in the US. Nat. Geosci. 3, 13–17. https://doi.org/10.1038/ngeo721 (2010).
Pregitzer, K. S., Burton, A. J., Zak, D. R. & Talhelm, A. F. Simulated chronic nitrogen deposition increases carbon storage in Northern Temperate forests. Glob. Change Biol. 14, 142–153. https://doi.org/10.1111/j.1365-2486.2007.01465.x (2008).
Hedwall, P.-O., Gong, P., Ingerslev, M. & Bergh, J. Fertilization in northern forests—Biological, economic and environmental constraints and possibilities. Scand. J. For. Res. 29, 301–311. https://doi.org/10.1080/02827581.2014.926096 (2014).
50Haugland, H. et al. Målrettet gjødsling av skog som klimatiltak – egnede arealer og miljøkriterier. Miljødirektoratet Rapportserie M174/2014 (2014).
Casas-Ruiz, J. P. et al. A tale of pipes and reactors: Controls on the in-stream dynamics of dissolved organic matter in rivers. Limnol. Oceanogr. 62, S85–S94. https://doi.org/10.1002/lno.10471 (2017).
Kaste, Ø. & Dillon, P. J. Inorganic nitrogen retention in acid-sensitive lakes in southern Norway and southern Ontario, Canada—A comparison of mass balance data with an empirical N retention model. Hydrol. Process. 17, 2393–2407. https://doi.org/10.1002/hyp.1249 (2003).
Kaste, Ø., Stoddard, J. L. & Henriksen, A. Implication of lake water residence time on the classification of Norwegian surface water sites into progressive stages of nitrogen saturation. Water Air Soil Pollut. 142, 409–424. https://doi.org/10.1023/A:1022015814800 (2003).
Kritzberg, E. S. Centennial-long trends of lake browning show major effect of afforestation. Limnol. Oceanogr. Lett. 2, 105–112 (2017).
Skarbøvik, E., Stålnacke, P., Kaste, Ø. & Austnes, K. Trends in nutrients and metals in Norwegian rivers and point sources 1990–2009. Hydrology Research 45, 441–454. https://doi.org/10.2166/nh.2013.233 (2013).
56Aas, W., Hjellbrekke, A.-G., Fagerli, H. & Benedictow, A. Deposition of major inorganic compounds in Norway 2012–2016. NILU-report 41 (2017).
Lussana, C., Tveito, O. E. & Uboldi, F. Three-dimensional spatial interpolation of 2 m temperature over Norway. Q. J. R. Meteorol. Soc. 144, 344–364. https://doi.org/10.1002/qj.3208 (2018).
Lussana, C. et al. seNorge2 daily precipitation, an observational gridded dataset over Norway from 1957 to the present day. Earth Syst. Sci. Data 10, 235–249. https://doi.org/10.5194/essd-10-235-2018 (2018).
Axler, R. P., Rose, C. & Tikkanen, C. A. Phytoplankton nutrient deficiency as related to atmosperhic nitrogen deposition in northern Minnesota acid-sensitive lakes. Can. J. Fish. Aquat. Sci. 51, 1281–1296. https://doi.org/10.1139/f94-128 (1994).
60Marchetto, A. rkt: Mann-Kendall Test, Seasonal and Regional Kendall Tests. R package version 1.5. (2017).
Zuur, A. F., Ieno, E. N., Walker, N. J., Savaeliev, A. A. & Smith, G. M. Mixed Effects Models and Extensions in Ecology with R (Springer, Berlin, 2009).
Zuur, A. F., Hilbe, J. M. & Ieno, E. N. A Beginner’s Guide to GLM and GLMM with R (Highland Statistics Ltd., Newburgh, 2015).
Wickham, H. ggplot2: Elegant Graphics for Data Analysis (Springer, Berlin, 2016).
Lüdecke, D. sjPlot: Data Visualization for Statistics in Social Science. (2020).
Acknowledgements
The river and deposition data used in this publication stem from the Norwegian river monitoring program [led by the Norwegian Institute for Water Research (NIVA)] and the Norwegian monitoring program on long-range transboundary air pollution [led by the Norwegian Institute for Air Research (NILU)], respectively, both funded by the Norwegian Environment Agency. The research of this publication was supported by NIVA’s strategic institute program on the topic land-ocean interactions. We would like to thank all discussion partners within this working group for providing a fruitful context to the authors. Further thanks to James Sample, Jose-Luis Guerrero Calidonio and Espen Lund for assistance with the input data.
Author information
Authors and Affiliations
Contributions
A.D. analysed the data with contributions from K.A., who compiled most input data and conducted load calculations. Ø.K. and H.F. contributed with valuable comments regarding the input data, and statistical analysis, respectively. A.D. wrote the manuscripts, where all co-authors commented on earlier versions of the draft. All authors read and approved the submission of this manuscript.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Deininger, A., Kaste, Ø., Frigstad, H. et al. Organic nitrogen steadily increasing in Norwegian rivers draining to the Skagerrak coast. Sci Rep 10, 18451 (2020). https://doi.org/10.1038/s41598-020-75532-5
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-020-75532-5
- Springer Nature Limited