Abstract
Circadian clocks improve plant fitness in a rhythmic environment. As each cell has its own circadian clock, we hypothesized that sets of cells with different functions would have distinct rhythmic behaviour. To test this, we investigated whether different organs in field-grown sugarcane follow the same rhythms in transcription. We assayed the transcriptomes of three organs during a day: leaf, a source organ; internodes 1 and 2, sink organs focused on cell division and elongation; and internode 5, a sink organ focused on sucrose storage. The leaf had twice as many rhythmic transcripts (>68%) as internodes, and the rhythmic transcriptomes of the internodes were more like each other than to those of the leaves. Among the transcripts expressed in all organs, only 7.4% showed the same rhythmic pattern. Surprisingly, the central oscillators of these organs — the networks that generate circadian rhythms — had similar dynamics, albeit with different amplitudes. The differences in rhythmic transcriptomes probably arise from amplitude differences in tissue-specific circadian clocks and different sensitivities to environmental cues, highlighted by the sampling under field conditions. The vast differences suggest that we must study tissue-specific circadian clocks in order to understand how the circadian clock increases the fitness of the whole plant.
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Introduction
The circadian clock is an endogenous signaling network that allows organisms to adapt to rhythmically changing environments. Plants with a circadian clock synchronized with environmental rhythms accumulate more biomass and have better fitness than plants with defective or without circadian clocks1,2. In crops, changes in the circadian clock have been indirectly selected through traditional breeding to change photoperiodic responses, such as the transition to flowering. For example, the circadian clocks of European Solanum lycopersicum L. (tomatoes) have longer periods than those of native American tomatoes, as such periods allow these crops to adapt better to the long summer days occurring at the high latitudes of much of Europe3. Similarly, some genotypes of Hordeum vulgare L. (barley) and Triticum aestivum L. (wheat) carry mutations in their circadian clock genes that reduce flowering induced by photoperiodic triggers, allowing cultivation in higher latitudes in Europe4,5.
The circadian clock is conceptually divided into three associated parts: the Input Pathways, the Central Oscillator, and the Output Pathways. The Input Pathways detect entraining cues that keep the circadian clock continuously synchronized to the environment. In plants, these cues include light, temperature, and sugar levels6,7,8. The Central Oscillator is a series of interlocking transcriptional-translational feedback loops that can generate 24-h rhythms independently of the environment. In Arabidopsis thaliana (L.) Heynh. (Arabidopsis), one loop, called the morning loop, starts with the light induction of CIRCADIAN CLOCK ASSOCIATED1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) at dawn. Next, PSEUDO-RESPONSE REGULATOR7 (PRR7) and PRR9 are activated by CCA1 and LHY. In turn, CCA1 and LHY are repressed by PRR7 and PRR9. In the core loop, CCA1 and LHY are repressed, and this represses TIME FOR CAB EXPRESSION1 (TOC1), also known as PRR1. During the night, TOC1 forms an interaction known as the evening loop with the EVENING COMPLEX (EC). The EC is a protein complex formed by EARLY FLOWERING3 (ELF3), ELF4, and LUX ARRHYTHMO (LUX) that also inhibits the expression of PRR7 and PRR9 the next morning. Other essential components of the oscillator include GIGANTEA (GI), REVEILLE8 (RVE8), and CCA1 HIKING EXPEDITION (CHE)8,9,10,11. The Output Pathways transduce the temporal information generated by the interaction between the Central Oscillator and the Input Pathways to a plethora of biochemical pathways. The circadian clock thus has a broad impact throughout the plant, regulating processes such as photosynthesis, cell elongation, stomata opening, and flowering12.
Even though the plant circadian clock is highly conserved, there are a few differences between the circadian clocks of Arabidopsis and grasses (Poales). For instance, there is only one copy of the paralogs CCA1/LHY, usually assigned as LHY13. The grass PRRs consist of TOC1, PRR37, PRR73, PRR59, and PRR95, and it is not clear whether they have the same functions as their Arabidopsis counterparts, even though they are capable of complementing Arabidopsis mutations13,14. In sugarcane, a highly polyploid crop that accumulates sucrose in the culm, the circadian clock has high-amplitude rhythms and regulates a large proportion of the leaf transcriptome (>30%)15,16.
Most research to date on plant circadian rhythms has been done under controlled conditions, inside a growth room or growth chamber. Under such circumstances, plants can be grown either under circadian conditions, in which they are kept under constant abiotic conditions as a means to separate endogenous rhythms from rhythms driven by the environment, or under diel conditions, in which they are subjected to abiotic rhythms such as light/dark and warm/cold. Abiotic changes in controlled conditions are usually stepwise, in contrast to the gradients found in natural or field conditions, which can lead to significant changes in plant physiology17,18,19. For example, different patterns of metabolite rhythms are observed if plants are grown under white fluorescent tubes, light-emitting diodes that simulate the sunlight spectrum, or naturally illuminated greenhouse18. In another study, the period and phase of the circadian clock affected shoot and rosette branch numbers in multiple Arabidopsis mutants under natural, but not under controlled, conditions20. Finally, the rice mutant osgi, which has a late-flowering phenotype in controlled conditions, flowered at the same time as the wild type in field conditions21.
Only two plant species have had their rhythmic transcripts identified in field conditions: rice and pineapple21,22,23,24. However, these studies focused on the leaves. To better understand how the plant circadian clock regulates transcription under natural conditions in different organs, we measured transcription in three organs of field-grown sugarcane grown during the day. We harvested leaf +1 (L1), a source organ, and two sink organs: internodes 1 and 2 (I1), organs focused on cell division and cell elongation that includes the shoot apical meristem; and internode 5 (I5), an organ focused on sucrose accumulation. We describe in detail one cycle (24 h) with 14 time points, starting 2 h before dawn. This approach allowed us to obtain a better resolution to describe transcripts with fast dynamics. We found that the rhythmic transcripts of the L1, I1, and I5 are widely specialized and likely to respond differently to environmental cues.
Results
A significant proportion of the sugarcane transcriptome is rhythmic in diel conditions
We planted a field of commercial sugarcane (Saccharum hybrid SP80-3280) in autumn 2012 in Araras (Brazil, 22°18′41.0″S, 47°23′05.0″W). Nine months later (summer 2013), after a dry winter and spring (Fig. S1), we did a time course experiment in which the leaf +1 (L1), internodes 1 and 2 (I1), and internode 5 (I5) were harvested every 2 h for 26 h, starting 2 h before dawn. On the day of harvest, the stalks were 76 ± 0.16 cm, with 11 ± 2 internodes, and their sugar content was 12.0 ± 1.4°Bx (mean ± SD; n = 20). The temperature varied throughout the day from 17°C to 30°C, with the maximum occurring at 11 h after dawn (ZT11); the maximum light intensity was 2.67 MJ/m2 at ZT07, and dusk occurred 13.25 h after dawn (ZT13.25) (Fig. S1B,D).
RNA extracted from each organ was hybridized in 44k custom oligoarrays15,25. The data from the time course experiment generated 14,521 time series with 14 time points. After the selection of time points that had a signal above the background noise (Fig. S3A), we had 12,501 transcripts considered to be expressed in at least one organ (Fig. 1). L1 had 9,822 expressed transcripts, 94.3% of them were also expressed in a previous circadian experiment15 (Fig. 1B). I1 had the highest number of expressed transcripts (12,053), followed by I5 (10,448). A total of 9,380 transcripts were expressed in all three organs (75.0%, Fig. 1E). I1 and I5 shared the most substantial proportion of the expressed transcripts (89.3%), and I1 had the most substantial proportion of unique expressed transcripts (7.5%).
We identified rhythmic transcripts by combining a weighted correlation network analysis (WGCNA) that grouped expressed transcripts in coexpression modules26 with JTK_CYCLE, which identified which of the modules contained rhythmic transcripts27 (Fig. S3B). This method identified 6,705 rhythmic transcripts in L1 (68.3%), 3,755 in I1 (31.2%), and 3,242 in I5 (28.8%) (Figs. 1A and S6). As a comparison, 32.1% of the transcripts were rhythmic in L1 under circadian conditions15. The overlap between circadian transcripts and rhythmic transcripts in the field (in diel conditions) was 2,623, representing 76.4% of circadian transcripts and 60.1% of rhythmic transcripts (Fig. 1C).
Different sets of transcripts are rhythmic in different sugarcane organs
Although most expressed transcripts were found in all three organs, only 1,413 of the expressed transcripts were rhythmic in all three organs (16.6%) (Fig. 1D,F). L1 had the largest proportion of unique rhythmic transcripts (41.5%), followed by I1 (8.5%) and then I5 (5.7%) (Fig. 1F). Transcripts that were expressed only in one organ were less likely to be rhythmic (60.3% for L1, 8.6% for I1, and 8.4% for I5) (Fig. 1H).
We estimated the phase of the transcripts by combining the phase calculated using JTK_CYCLE with a dendrogram of the representative time course of each module. Among the transcripts that were rhythmic in more than one organ, 27% had rhythms with phase differences >2 h (Fig. 1D). Overall, among the 12,501 unique expressed transcripts in the three organs, only 7.4% (923) showed rhythms with the same phase in the three organs. Most of the transcripts peaked during the day: this was true of 80.3% in L1, 90.4% in I1, and 96.3% in I5 (the photoperiod was 13.25 h, or 56.3% of a cycle) (Fig. 1G). In L1, 2,363 transcripts peaked between dawn (ZT00) and 2 h after dawn (ZT02) (35.2%), and 1,232 transcripts peaked at ZT12 (18.4%) (Fig. 2A). When we separated rhythmic L1 transcripts into those that were also rhythmic in circadian conditions (Fig. 2B, α) and those that were not (Fig. 2B, β), two different phase distributions could be observed (Fig. 2C). The α group had most transcripts peaking at ZT00-02 (39.1%), followed by ZT12 (14.0%), while the β group peaked at ZT12 (25.1%), followed by ZT02 (19.1%). In I1, 1,201 transcripts peaked at ZT0 (32.0%) and 716 peaked at ZT8 (19.1%). In I5, 1,373 transcripts peaked at ZT0 (42.4%) and 894 peaked at ZT8 (27.6%) (Fig. 2A).
The majority of transcripts from L1 (65.8%) grown under diel conditions had the same phase (± 2 h) in leaves grown under circadian conditions (Fig. S7A). More transcripts showed a delayed peak (19.6%) rather than an advanced peak (13.9%) under diel conditions than under circadian conditions. When we compared L1 and I1 transcripts, 65.8% had the same phase, with the remainder divided roughly evenly between delayed and advanced phases (16.1% and 14.9%, respectively) (Fig. S7B). Similarly, 67.1% of the L1 transcripts had the same phase as I5, 14.2% had a delayed phase, and 14.8% had an advanced phase (Fig. S7C). The phases were most similar between I1 and I5 transcripts: 93.8% had the same phases, 2.8% a phase delay, and 3.1% a phase advance (Fig. S7D).
Biochemical pathways have different rhythms in sugarcane organs
We used a hypergeometric test to detect if a pathway was over- or underrepresented by comparing the frequency of transcripts associated with a Biochemical Pathway among the expressed transcripts and all the unique transcripts in the oligoarray (Fig. 2D and Fig.). We used the same test comparing the frequency of transcripts associated with a Biochemical Pathway among the rhythmic transcripts and the expressed transcripts (Figs. 2D and S8). The transcript annotations were based on the SUCEST database annotation (http://sucest-fun.org).
Among the expressed transcripts, each organ has a distinct profile. For example, L1 was the only organ that had Pigment Synthesis, Light Harvesting, and Jasmonate Signaling pathways considered to be overrepresented. I1 had Chromatin Remodeling and Protein Synthesis pathways overrepresented and Ethylene Signaling underrepresented. Transcription Factors was underrepresented and ABA/Drought Signaling and Transporters were overrepresented in L1 and I5, but not in I1. I5 is the only organ in which Cell Wall Synthesis & Elongation was not underrepresented among the expressed transcripts (Fig. 2D). Among the rhythmic transcripts, Circadian Clock was overrepresented, while Chromatin Remodeling and RNA Synthesis & Processing were underrepresented in all organs. Protein Synthesis was overrepresented in L1. Transcription Factors was overrepresented in I1 and I5, and Transporters was overrepresented among rhythmic transcripts in L1 and I1 (Fig. 2D).
When we analyzed transcripts associated with important pathways for sugarcane growth, we found further organ-specific patterns; these differences could be seen in both expressed and rhythmic transcripts, as well as the phase of the rhythmic transcripts (Figs. 2E and S9). Transcripts associated with Carbohydrate Metabolism tended to peak in the morning. Almost half (48.0%) of the transcripts had a peak at ZT00 in L1, while the majority peaked between ZT00 and ZT04 in both I1 (53.2%) and I5 (58%) (Fig. 2E). Amongst the individual transcripts, a putative ortholog of SUCROSE SYNTHASE4 (SuSy4) had a similar rhythmic pattern in all three organs. A putative ortholog of SUCROSE-PHOSPHATE SYNTHASE II (SPSII) was rhythmic only in L1, while a putative ortholog of a CELL WALL INVERTASE (CWI) exhibited a sharp peak at ZT04 in L1 but a very broad peak at ZT08 in I1 and I5 (Fig. S9I,M, and Q).
Transcripts associated with Cell Wall Synthesis & Elongation had a more diverse phase distribution: in L1, 55% had a peak between ZT00 and ZT04; in I1, 73.4% had a peak between ZT00 and ZT08; and in I5, 45.8% had a peak between ZT08 and ZT10 and 37.8% had one at ZT00 (Fig. 2E). There was also a higher proportion of transcripts associated with Cell Wall Synthesis & Elongation that are expressed only in I1 and I5 (Fig. S9). Transcripts associated with Amino Acid Metabolism peaked between ZT12 and ZT14 in L1 (50%). In I1 and I5, they had two peaks: between ZT00 and ZT02 (37.5% and 57.1%) and between ZT08 and ZT10 (37.5% and 42.9%) (Fig. 2B). Transcripts associated with Transporters peaked at ZT02 (35.7%) and ZT12 (15.7%) in L1. In I1, most of the transcripts peaked 2 h earlier, at ZT00 (24.2%) and ZT10 (24.2%). I5 displayed a similar pattern to I1, with 53.6% peaking between ZT00 and ZT02 and 46.4% between ZT08 and ZT10 (Fig. 2B). This tendency for L1 to have later phases than I1 and I5 can be seen in the putative ortholog SWEET2, which peaked at ZT02 in L1 and at ZT18-20 in I1 and I5 (Fig. S9L).
Circadian clock transcripts have similar dynamics in different sugarcane organs
The differences in the rhythmic transcripts of the three organs could be explained by the presence of organ-specific circadian clocks that could generate different patterns of rhythmic transcription. For this reason, we looked at rhythms in Input Pathways, Central Oscillator, and Output Pathways of the circadian clock. Most of the known Input Pathways to the circadian clock are associated with Light Signaling6. Light Signaling is underrepresented among the transcripts expressed in I5 and the rhythmic transcripts in I1 (Fig. S8). Among the red light receptor genes, PHYTOCHROME A.1 (PHYA.1) was rhythmic in L1, with a peak at ZT23, while PHYB was not rhythmic in any organ (Fig. S10A,B). In I1 and I5, both PHYs had two peaks: one near dawn (ZT00-02) and another at night (ZT18-20). Among the blue light receptors, CRYPTOCHROME1.1 (CRY1.1) was rhythmic in L1, peaking at ZT03. CRY2.1 was rhythmic in I5, peaking at ZT19. ZEITLUPE (ZTL.1) was rhythmic in L1 and I5, peaking at ZT01 and ZT21, respectively (Fig. S10D–F).
The transcripts associated with the Central Oscillator displayed rhythms with similar dynamics (Fig. 3). LHY peaked early in the morning, between ZT02 and ZT04, with overlapping dynamics in all three organs (Fig. 3a). Similarly, TOC1 peaked around dusk, between ZT10 and ZT12, in all three organs (Fig. 3D). The normalizations used to analyze the oligoarray data do not allow the comparison of expression levels, so we used RT-qPCR to show that LHY varied during the day by 750× in L1 and 150× in I1 and I5 (Fig. S11S). In contrast, TOC1 differed 30× in L1 and 18× in I1 and I5 (Fig. S11B). The other PRR genes, PRR59, PRR73, and PRR95 (referred to as ScPRR3, ScPRR7, and ScPRR59, respectively, in Hotta et al., 2011), peaked between ZT06 and ZT10 (Fig. 3B,C and E). GI peaked between ZT08 and ZT10 in all three organs (Fig. 3F). Finally, ELF3 was rhythmic only in L1, with a peak at ZT14. In the internodes, ELF3 had a similar pattern, but it was not regarded as rhythmic due to high noise (Fig. S11C).
Among the possible pathways that can be recruited by the circadian clock that are considered part of the Output Pathways are those associated with Chromatin Remodeling, Transcription Factors, and Protein Synthesis (Fig. 4). Transcripts associated with Chromatin Remodeling peaked at ZT00-02 and ZT10-12 in L1 (32.5% and 36.5%, respectively). In I1 and I5, they peaked at ZT00 (40.7% and 40.8%, respectively) and ZT08-10 (33.99% and 51.2%, respectively) (Fig. 4A). Transcripts associated with Transcription Factors tended to peak near dawn, at ZT00-02, in all three organs (57.5% in L1, 46.4% in i1, and 50.3% in I5). A higher proportion (22.6%) of transcripts associated with Transcription Factors were rhythmic when compared to all rhythmic (16.6%) transcripts, χ2(6, n = 341) = 15.1, P = 0.02 (chi-square test, Fig. 4E). These transcripts also peaked similarly in all organs: 79.3% peaked in the same interval in L1 as in I1, 72.2% peaked in the same interval in L1 and I5, and 93.1% in I1 and I5.
Transcripts associated with Protein Synthesis tended to peak at dusk in L1 (ZT12, 49.0%), at dawn and afternoon in I1 (ZT00, 36.1%; ZT10, 32.5%), and at dawn in I5 (ZT00, 61.7%) (Fig. 4C). A high proportion of transcripts associated with Protein Synthesis were expressed in all three organs (91.4%) (Fig. 4F). In contrast, more than half of the transcripts (54.6%) were rhythmic only in L1, whereas a lower frequency (41.5%) of total rhythmic transcripts were seen only in L1, χ2(6, n = 269) = 34.8, P < 0.001 (Fig. 4I).
Other transcripts showed a wide variety of oscillations amongst the three organs (Fig. 4J–L, and S11). The putative STRUCTURAL MAINTENANCE OF CHROMOSOMES1 (SMC1), associated with Chromatin Remodeling, peaked at ZT06 in L1 and ZT11 in I1 and I5 (Fig. 4J). Two putative JUMONJI-C (JMJC) DOMAIN-CONTAINING PROTEIN5 (JMJD5) genes, encoding proteins that can act as histone demethylases, were found in sugarcane. JMJD5.1 is expressed only in I1 and I5 and has a phase at ZT10 (Fig. S12A,D); JMJD5.2 is expressed in all organs with similar rhythmic patterns (Fig. S12A). The transcription factor gene HOMEOBOX PROTEIN24 (HB24) is rhythmic only in L1, with a peak at ZT10 (Fig. 4K). Another rhythmic gene, 40 S RIBOSOMAL PROTEIN S15 (S15A), associated with Protein Synthesis, has a peak at ZT14 in L1 and at ZT00 in I1 and I5 (Fig. 4L).
Discussion
Organ-specific rhythms of transcription can be found in highly productive and intensively selected commercial sugarcane. The specialization of the rhythmic transcriptome may help the plant cells to adapt to local environmental rhythms, as well as to generate rhythms that are compatible with their specialized needs. Specialized rhythms may also be essential to rhythmic processes that require organ-to-organ coordination, such as sucrose transport from the leaves to the internodes28.
Rhythms in field conditions are different from those in controlled conditions
Sugarcane leaves in field conditions had twice as many transcripts identified as rhythmic than plants assayed under circadian conditions. This difference is expected because some rhythms are driven by environmental oscillations, such as light and temperature. Also, some circadian-clock-driven rhythms may undergo amplitude increases due to a general increase in the amplitude of the Central Oscillator. In L1, the transcriptional rhythms of LHY vary by up to 60× in a day in circadian conditions and 750× in field conditions, while those of TOC1 vary up to 5× in a day in circadian conditions and 40× in field conditions (Hotta et al., 2013, and Fig. S11).
Under circadian conditions, most transcripts peaked at subjective dusk (ZT12, 29.0%), which resulted in 60.5% of the transcripts peaking during subjective night. By contrast, under field conditions, most transcripts peaked near dawn (ZT00-02, 35.2%) in L1, which resulted in 80.3% of the transcripts peaking during the day. This reinforces the role of the light/dark transition as the driving force of rhythms in leaves under field conditions. A high proportion (64.1%) of the transcripts that peaked during the subjective night in circadian conditions showed phase changes that made their peak happen during the day in field conditions. This might suggest the existence of dampening mechanisms that actively decrease nocturnal peaks. A similar mechanism keeps cytoplasmic calcium concentration lower during the night under diel conditions (day/night) than during the subjective night under circadian conditions29. Most of the transcripts associated with the Central Oscillator maintained their core phases, except LHY, which had a later peak (ZT01 under circadian conditions; ZT04 in field conditions). As a comparison, LHY is induced by light in Arabidopsis and is mostly insensitive to temperature in rice22,30. In sugarcane, alternative splicing of LHY correlates with environmental temperature31. The differences between the rhythmic transcriptomes in circadian conditions and field conditions highlight the importance of experiments done under field conditions to understanding how the circadian clock can affect the plant transcriptome in natura. For example, simulations of natural conditions in growth chambers showed that the flowering signal FLOWERING LOCUS T (FT) has a different phase under such conditions than it does under controlled conditions in Arabidopsis19. This discovery will require adjustments to the current flowering models to reflect events in natural conditions.
In recent years, the productivity gains of sugarcane crop through classical breeding has been decreasing32,33. A possible strategy to increase productivity gains is the use of molecular markers33,34,35. However, the association between genotype and phenotype remains a challenge, despite many attempts36,37. Several studies have identified drought-induced genes in order to identify targets for molecular breeding25,38,39,40. However, as most of these studies only harvest at one timepoint, it is possible that important rhythmic drought-induced genes are missed41. In addition, delays in the harvesting of plant material, or changes in phase or period of rhythmic genes, can lead to the genes to be incorrectly considered differentially expressed42. Thus, the identification of rhythmic genes in the field can both increase the identification of genes of interest and help to reduce the number of false positive, aiding the identification of targets for molecular breeding.
Rhythmic transcripts are organ-specific
The transcripts in L1, I1, and I5 have very different rhythmic patterns, even though most of the expressed transcripts were found in all three organs. Rhythms in I1 and I5 were more like each other than to those in L1, and only 7.4% of the transcripts expressed in all three organs showed the same rhythms. Thus, we conclude that these three organs have vastly different and specialized circadian clocks. These specialized circadian clocks could be the result of multiple organ sensitivities to environmental cues, of organ-specific Core Oscillators, and of organ-specific interactions of Output Pathways with environmental signals43,44.
In Arabidopsis, different sensitivities to environmental cues are found in the vascular phloem companion cells, which are more sensitive to photoperiodism, and the epidermis cells, which are more sensitive to temperature43. In sugarcane, most L1 transcripts peak at ZT00-02 and ZT12, following dawn and dusk, while most I1 and I5 transcripts peak at ZT00 and ZT08, following dawn and the daily light and temperature maxima. Thus, the circadian clocks of these organs respond differently to environmental cues such as photoperiod, light/dark transition, or temperature, as in Arabidopsis. In rice, a significant proportion of rhythmic transcripts are regulated either by the circadian clock or by temperature oscillations22. In sugarcane, rhythmic L1 transcripts that were also rhythmic in circadian conditions had peaks that follow LHY or TOC1 expression. On the other hand, rhythmic L1 transcripts that were not rhythmic in circadian conditions peaked at dawn and dusk. In internodes, transcripts peaked at dawn and at the light and temperature maxima. Such organ-specific sensitivity to environmental cues was previously described in the vasculature and leaf epidermis43.
The Central Oscillators of mesophyll and vasculature in Arabidopsis have similar components but with different amplitudes. AtELF4 rhythms have an amplitude 10× higher in the vasculature, AtPRR7 and AtPRR9 amplitudes are 2× higher in the mesophyll, and AtTOC1 amplitude is analogous in both tissues45. In sugarcane, LHY amplitude is 6× higher and TOC1 amplitude is 2× higher in L1 compared to I1 and I5. As leaves are exposed to direct sunlight, whereas internodes are protected by layers of leaf sheaths, it is probable that sunlight is responsible for these amplitude differences. The dynamics of LHY, TOC1, and GI during the day were very similar in the three organs. Indeed, they were considered to be coexpressed when analyzed together (data not shown). As the three organs have different levels of exposure to the environment, the existence of a common environmental signal is unlikely. Alternatively, the oscillators of the three organs could be coupled. There is evidence in Arabidopsis of root oscillators being regulated by the oscillators of the aerial parts of the plants, either the leaves or the shoot apical meristem (SAM)46,47. As the leaves are a source signal to both internodes, it is possible that synchronizing signals are transported with sucrose and other sugars. In Arabidopsis, sugars can also act as an entrainment signal7,48.
Even though there is much evidence for tissue-specific circadian clocks in Arabidopsis29,45,49,50,51, less is known about their effect on the rhythmic regulation of transcripts. In contrast, tissue-specific rhythms have been widely studied in mammals52,53,54,55. Sampling of 12 different mouse organs over time showed that 43% (~8,500) of all transcripts had circadian rhythms in at least one organ, but only 10 transcripts were rhythmic in all organs54. As in sugarcane leaves, the rhythmic transcripts in mammalian organs tended to peak at dawn and dusk. In general, the only transcripts that had similar phases across all organs were the ones associated with the mammalian Core Oscillator54.
At least two regulatory pathways are required to generate tissue-specific sets of rhythmic transcripts: one that confers organ specificity and one that confers rhythmicity. These pathways can be organized in different nonexclusive ways: they could act on a gene independently, the tissue specificity pathways could regulate the rhythmicity pathways, or the rhythmicity pathways could regulate the organ specificity pathways (Fig. S13). The rhythmicity pathways can be dependent on the circadian clock, on environmental rhythms, or both. The tissue specificity pathways can include transcription factors, protein-protein interactions, alternative promoter usage, and chromatin interactions56.
In our datasets, transcripts that were expressed only in one organ or only in the internodes were less likely to be considered rhythmic (Fig. 2B). Thus, it is possible that rhythmic pathways regulate only a small proportion of organ-specific pathways. Transcripts associated with Transcription Factors were more likely to be rhythmic in all three organs, and these transcripts had a higher probability of having the same phase. However, just a few tissue-specific rhythms in transcription factors can have a sizeable cascading effect57. Tissue-specific transcription factors, even if arrhythmic, could also change the phase of rhythmic transcripts through protein-protein interactions or by changing the promoter usage56,58. Finally, chromatin remodeling could be a significant regulatory pathway in the generation of the tissue-specific rhythmic transcriptome. In Arabidopsis, chromatin remodeling can regulate the Central Oscillator, but little is known about how the plant circadian clock can use chromatin remodeling to generate rhythms59,60,61,62. In sugarcane, rhythms in transcripts associated with Chromatin Remodeling were underrepresented among the rhythmic transcripts. However, chromatin remodeling tends to be regulated post-transcriptionally through histone modifications. Transcription can also be regulated at the chromatin level through topologically associating domains (TADs). TADs are domains of DNA that self-interact, generating regulatory compartments within the chromosomes63. An enhancer only interacts with a gene if they share the same TAD. In consequence, it is possible to change the enhancers that interact with a gene by changing the boundaries of a TAD, which are maintained by cohesins and CCCTC-binding factors (CTCF) in mammals63. TADs can be regulated to generate tissue-specific transcription and even rhythms64,65,66. In plants, TADs are maintained by cohesins, but there are still no known CTCF counterparts67,68. In sugarcane, the cohesin subunit SMC1 has different phases in leaves and internodes (Fig. 4J).
The role of organ-specific rhythms in sugarcane
Organ-specific rhythms may affect sugarcane productivity as they allow the different tissues to be more efficient according to their function and local environmental rhythms56. In mammals, rhythms in fibroblasts allow wound healing to occur faster during the active phase than the rest phase69. Rhythms in the liver lead to larger cell sizes and higher protein levels during the active phase and after feeding, making detoxification more efficient during the active and post-feeding periods70.
Nutrient and photoassimilate transportation inside the plant is essential for rhythmic processes and may also be part of organ-to-organ coordination and C partitioning28,71,72,73. In sugarcane, expressed transcripts associated with Transporters were overrepresented in L1 and I5, while rhythmic transcripts associated with Transporters were overrepresented in L1 and I1. Furthermore, transcripts associated with Transporters tended to peak 2 h later in L1 than in the internodes, which may indicate that the latter is the driving force of this process. The phloem and xylem are also important organs for the integration of multiple rhythmic information generated by specialized circadian clocks, such as flowering74.
Sugarcane have rhythms of sucrose and starch in the leaves but not in the internodes75. In this crop, sucrose is synthesized in the leaves and is degraded in the apoplast or cytosol of internodes to be re-synthetized in their vacuoles28. Organ-specific regulation of transcripts may regulate sucrose storage in sugarcane. Differences in the rhythms of transcripts associated with Carbohydrate metabolism may be a way to regulate C partitioning to different organs. In our experiments, transcripts associated with Carbohydrate metabolism peaked later in internodes than in the leaves (Fig. 2E). CWI had a peak at ZT04 in L1, and at ZT08 in I1 and I5 (Fig. S9Q). In sugarcane, higher activities of cell wall invertases are associated with higher sucrose content, possibly by enhancing sucrose unloading in the internodes28,76,77,78. SPSII, one of the enzymes that synthesize sucrose, was only rhythmic in L1, with a morning peak (Fig. S9M). Interestingly, two rhythmic SuSy had the same dynamics in all organs (Fig. S9I) but the other four were only expressed in the internodes. Sucrose synthases can function either degrading or synthesizing sucrose. In sugarcane, SuSys mainly work in the degradative direction, and their pattern of expression are associated with the regulation of sucrose uptake in the internodes28,79,80.
Conclusions
The vast differences found in the rhythmic transcriptomes of different plant organs provide important clues to understanding the way that tissue-specific circadian clocks are generated and their impact on plant physiology. However, little is still known about the molecular mechanisms that control this specialization. The combination of organ- or tissue-specific studies with the observation of rhythms in the field, where conditions are fluctuating and variable as is normal in natural environments, is essential to understanding the nuances of how the plant circadian clock increases the fitness of plants and, in turn, crop productivity.
Materials and Methods
Plant growth and harvesting
Commercial sugarcane (Saccharum hybrid SP80-3280) was planted in a field in Araras, Brazil (22°18′41.0″S, 47°23′05.0″W, at an altitude of 611 m), in April 2012 (autumn) (Fig. S1). The soil on the site was a Typic Eutroferric Red Latosol. Plants were harvested 9 months later, in January 2013 (summer), after an unusually dry winter and spring. The time course experiment started 2 h before dawn and continued every 2 h until the next dawn, generating time series with 14 time points in total. Dawn was at 5:45, and dusk was at 19:00 (13.25 h light/10.75 h dark) (Fig. S1). At each time point, leaf +1 (the first leaf from the top with clearly visible dewlap), internodes 1 and 2, and internode 5 of nine individuals were harvested (Fig. S2), frozen in liquid N2, and stored in three pools of three individuals each. Two pools were used as biological replicates for oligoarrays, and one pool was used for validation using the reverse-transcription quantitative PCR (RT-qPCR).
Oligoarray hybridizations
All frozen samples were pulverized in dry ice using a coffee grinder (Model DCG-20, Cuisinart, China). One hundred milligrams of each pulverized sample was used for extraction of total RNA using Trizol (Life Technologies), following the supplier’s instructions. The RNA was treated with 2 U DNase I (Life Technologies) for 30 min at 37 °C and cleaned using the RNeasy Plant Mini kit (Qiagen). The quality and quantity of RNA were assayed using an Agilent RNA 6000 Nano Kit Bioanalyzer chip (Agilent Technologies). Sample labeling was done following the Low Input Quick Amp Labelling protocol of the Two-Color Microarray-Based Gene Expression Analysis system (Agilent Technologies). Hybridizations were done using a custom 4×44 k oligoarray (Agilent Technologies) that was previously described15,25. Two hybridizations were done for each time point against an equimolar pool of all samples of each organ. Each duplicate was prepared independently using dye swaps. Data were extracted using the Feature Extraction software (Agilent Technologies) (Fig. S3A). Background correction was applied to each dataset. A nonlinear LOWESS normalization was also applied to the datasets to minimize variations due to experimental manipulation. Signals that were distinguishable from the local background signal were taken as an indication that the corresponding transcript was expressed. We have validated 10 transcripts (30 time series) using RT-qPCR (Fig S4 and S5). Among the time series identified as rhythmic (n = 23), 91% were also rhythmic using data from RT-qPCR (Table S2), and 77% were considered correlated using Spearman’s rank correlation coefficient. Among the time series identified as not rhythmic (n = 7), 86% were also not rhythmic using data from RT-qPCR, and 36.7% were considered correlated using Spearman’s rank correlation coefficient. The GenBank ID and Sugarcane Assembled Sequences (SAS) numbers for sugarcane genes are listed in Table S1. The complete dataset can be found at the Gene Expression Omnibus public database under the accession number GSE129543.
Data analysis
For the purposes of further analysis, only transcripts that were found to be expressed in more than 7 of the 14 time points were considered to be expressed. All of the expressed transcripts time series were grouped in coexpressed modules using the R package weighted correlation network analysis (WGCNA) to identify rhythmic transcripts26 (Fig. S3B). Network adjacency was calculated using a soft thresholding power of 18 for all organs. Modules that had a dissimilarity value of ≤ 0.25 were merged. Final modules were generated using a 0.175 adjacency threshold. As WGCNA groups together time series that have a positive or a negative correlation, we normalized each time series using a Z-score, separated these time series into two new modules, and generated a typical time series for each module by finding the median of all time series. Then, each representative time series was classified as rhythmic or non-rhythmic using JTK-CYCLE27. Modules that had an adjusted P-value of <0.75 were considered rhythmic. Finally, we filtered out noisy time series, defined as those that had a Spearman’s rank correlation coefficient of <0.3 when compared against the representative time series. Phase was assigned using the phase estimated by JTK-CYCLE corrected against a dendrogram with the representative time series of all modules of all organs. Modules that clustered together in the dendrogram were considered to have the same phase. The phase of a time series is defined as the time between dawn and the peak of the time course. Euler diagrams were done using the R package eulerr. Chi-squared (χ2) tests were used to compare Euler diagrams. Heatmaps were created using the R packages circlize81 and ComplexHeatmap82. To evaluate if a group of transcripts were under- or overrepresented, we used a hypergeometric test (phyper function in R). With this test, a P-value <0.05 suggests that the analyzed group is overrepresented in the dataset, while a P-value> 0.95 suggests that the analyzed group is underrepresented in the dataset. Code to fully reproduce our analysis is available on GitHub (https://github.com/LabHotta/sugarcane_field_rhytms) and archived on Zenodo (https://doi.org/10.5281/zenodo.2636813).
RT-qPCR analysis
As described for the oligoarray hybridizations, 100 mg of the pulverized frozen samples for all three organs was used for total RNA extractions following the same Trizol (Life Technologies) protocol and then were treated with DNase I (Life Technologies) and cleansed using the RNeasy Plant Mini Kit (Qiagen). RNA quality and concentration of each sample were checked using an Agilent RNA 6000 Nano Kit Bioanalyzer chip (Agilent Technologies). Five micrograms of total purified RNA was enough for the reverse transcription reactions using the SuperScript III First-Strand Synthesis System for RT-PCR (Life Technologies). The RT-qPCR reactions for all samples were done using Power SYBR Green PCR Master Mix (Applied Biosystems), 10× diluted cDNA, and specific primers described by Hotta et al. (2013) (Fig. S11). Reactions were placed in 96-well plates and read with the Fast 7500/7500 Real-Time PCR System (Applied Biosystems). Data analysis was performed using the Fast 7500/7500 Real-Time PCR System built-in software (Applied Biosystems).
References
Green, R. M., Tingay, S., Wang, Z.-Y. & Tobin, E. M. Circadian rhythms confer a higher level of fitness to Arabidopsis plants. Plant Physiol. 129, 576–584 (2002).
Dodd, A. N. et al. Plant circadian clocks increase photosynthesis, growth, survival, and competitive advantage. Science (New York, N.Y.) 309, 630–3 (2005).
Muller, N. A. et al. Domestication selected for deceleration of the circadian clock in cultivated tomato. Nat Genet 48, 89–93 (2016).
Turner, A., Beales, J., Faure, S., Dunford, R. P. & Laurie, D. A. The pseudo-response regulator Ppd-H1 provides adaptation to photoperiod in barley. Science 310, 1031–1034 (2005).
Gawroński, P. et al. A distorted circadian clock causes early flowering and temperature-dependent variation in spike development in the Eps-3Am mutant of einkorn wheat. Genetics 196, 1253–1261 (2014).
Oakenfull, R. J. & Davis, S. J. Shining a light on the Arabidopsis circadian clock. Plant Cell Environ. 40, 2571–2585 (2017).
Frank, A. et al. Circadian entrainment in Arabidopsis by the sugar-responsive transcription factor bZIP63. Current Biology 28, 2597–2606.e6 (2018).
Webb, A. A. R., Seki, M., Satake, A. & Caldana, C. Continuous dynamic adjustment of the plant circadian oscillator. Nature Communications 10, 550 (2019).
Hsu, P. Y., Devisetty, U. K. & Harmer, S. L. Accurate timekeeping is controlled by a cycling activator in Arabidopsis. Elife 2, e00473 (2013).
Millar, A. J. The intracellular dynamics of circadian clocks reach for the light of ecology and evolution. Annu Rev Plant Biol 67, 595–618 (2016).
Henriques, R., Papdi, C., Ahmad, Z. & Bögre, L. Circadian regulation of plant growth. In Annual Plant Reviews online 1–29 (American Cancer Society, https://doi.org/10.1002/9781119312994.apr0655. 2018).
Hotta, C. T. et al. Modulation of environmental responses of plants by circadian clocks. Plant, cell & environment 30, 333–49 (2007).
Calixto, C. P. G., Waugh, R. & Brown, J. W. S. Evolutionary relationships among barley and Arabidopsis core circadian clock and clock-associated genes. J. Mol. Evol. 80, 108–119 (2015).
Kusakina, J. et al. Barley Hv CIRCADIAN CLOCK ASSOCIATED 1 and Hv PHOTOPERIOD H1 are circadian regulators that can affect circadian rhythms in Arabidopsis. PLoS ONE 10, e0127449 (2015).
Hotta, C. T., Nishiyama, M. Y. & Souza, G. M. Circadian rhythms of sense and antisense transcription in sugarcane, a highly polyploid crop. PloS one 8, e71847 (2013).
Glassop, D. & Rae, A. L. Expression of sugarcane genes associated with perception of photoperiod and floral induction reveals cycling over a 24-hour period. Functional Plant Biol. 46, 314–327 (2019).
Shalit-Kaneh, A., Kumimoto, R. W., Filkov, V. & Harmer, S. L. Multiple feedback loops of the Arabidopsis circadian clock provide rhythmic robustness across environmental conditions. Proc. Natl. Acad. Sci. USA 115, 7147–7152 (2018).
Annunziata, M. G. et al. Response of Arabidopsis primary metabolism and circadian clock to low night temperature in a natural light environment. J. Exp. Bot. 69, 4881–4895 (2018).
Song, Y. H. et al. Molecular basis of flowering under natural long-day conditions in Arabidopsis. Nat Plants 4, 824–835 (2018).
Rubin, M. J. et al. Circadian rhythms vary over the growing season and correlate with fitness components. Mol. Ecol. 26, 5528–5540 (2017).
Izawa, T. et al. Os-GIGANTEA confers robust diurnal rhythms on the global transcriptome of rice in the field. Plant Cell 23, 1741–1755 (2011).
Nagano, A. J. et al. Deciphering and prediction of transcriptome dynamics under fluctuating field conditions. Cell 151, 1358–1369 (2012).
Matsuzaki, J., Kawahara, Y. & Izawa, T. Punctual transcriptional regulation by the rice circadian clock under fluctuating field conditions. Plant Cell 27, 633–648 (2015).
Ming, R. et al. The pineapple genome and the evolution of CAM photosynthesis. Nat. Genet. 47, 1435–1442 (2015).
Lembke, C. G., Nishiyama, M. Y., Sato, P. M., de Andrade, R. F. & Souza, G. M. Identification of sense and antisense transcripts regulated by drought in sugarcane. Plant Mol Biol 79, 461–477 (2012).
Langfelder, P. & Horvath, S. WGCNA: an R package for weighted correlation network analysis. BMC Bioinformatics 9, 559 (2008).
Hughes, M. E., Hogenesch, J. B. & Kornacker, K. JTK_CYCLE: an efficient nonparametric algorithm for detecting rhythmic components in genome-scale data sets. J Biol Rhythms 25, 372–380 (2010).
Wang, J., Nayak, S., Koch, K. & Ming, R. Carbon partitioning in sugarcane (Saccharum species). Front. Plant Sci. 4, (2013).
Xu, X. et al. Distinct light and clock modulation of cytosolic free Ca2+ oscillations and rhythmic CHLOROPHYLL A/B BINDING PROTEIN2 promoter activity in Arabidopsis. The Plant cell 19, 3474–90 (2007).
Kim, J.-Y., Song, H.-R., Taylor, B. L. & Carré, I. A. Light‐regulated translation mediates gated induction of the Arabidopsis clock protein LHY. The EMBO Journal 22, 935–944 (2003).
Dantas, L. L. B. et al. Alternative Splicing of Circadian Clock Genes Correlates With Temperature in Field-Grown Sugarcane. Front. Plant Sci. 10, (2019).
Waclawovsky, A. J., Sato, P. M., Lembke, C. G., Moore, P. H. & Souza, G. M. Sugarcane for bioenergy production: an assessment of yield and regulation of sucrose content. Plant Biotechnology Journal 8, 263–276 (2010).
Barreto, F. Z. et al. A genome-wide association study identified loci for yield component traits in sugarcane (Saccharum spp.). PLoS One 14, (2019).
Dal-Bianco, M. et al. Sugarcane improvement: how far can we go? Current opinion in biotechnology 23, 265–70 (2012).
Hotta, C. T. et al. The Biotechnology Roadmap for Sugarcane Improvement. Tropical Plant Biol. 3, 75–87 (2010).
Margarido, G. R. A., Pastina, M. M., Souza, A. P. & Garcia, A. A. F. Multi-trait multi-environment quantitative trait loci mapping for a sugarcane commercial cross provides insights on the inheritance of important traits. Mol Breed 35, (2015).
Balsalobre, T. W. A. et al. GBS-based single dosage markers for linkage and QTL mapping allow gene mining for yield-related traits in sugarcane. BMC Genomics 18, (2017).
Vantini, J. S. et al. Differential gene expression in drought-tolerant sugarcane roots. Genet. Mol. Res. 14, 7196–7207 (2015).
Gentile, A., Dias, L. I., Mattos, R. S., Ferreira, T. H. & Menossi, M. MicroRNAs and drought responses in sugarcane. Front Plant Sci 6, 58 (2015).
Li, C. et al. Differential expression profiles and pathways of genes in sugarcane leaf at elongation stage in response to drought stress. Sci Rep 6, 25698 (2016).
Dubois, M., Claeys, H., Broeck, L. Vden & Inzé, D. Time of day determines Arabidopsis transcriptome and growth dynamics under mild drought. Plant, Cell &. Environment 40, 180–189 (2017).
Hsu, P. Y. & Harmer, S. L. Circadian Phase Has Profound Effects on Differential Expression Analysis. PLOS ONE 7, e49853 (2012).
Shimizu, H. et al. Decentralized circadian clocks process thermal and photoperiodic cues in specific tissues. Nat Plants 1, 15163 (2015).
Bordage, S., Sullivan, S., Laird, J., Millar, A. J. & Nimmo, H. G. Organ specificity in the plant circadian system is explained by different light inputs to the shoot and root clocks. New Phytol. 212, 136–149 (2016).
Endo, M., Shimizu, H., Nohales, M. A., Araki, T. & Kay, S. A. Tissue-specific clocks in Arabidopsis show asymmetric coupling. Nature 515, 419–22 (2014).
James, A. B. et al. The circadian clock in Arabidopsis roots is a simplified slave version of the clock in shoots. Science (New York, N.Y.) 322, 1832–5 (2008).
Takahashi, N., Hirata, Y., Aihara, K. & Mas, P. A hierarchical multi-oscillator network orchestrates the Arabidopsis circadian system. Cell 163, 148–159 (2015).
Haydon, M. J., Mielczarek, O., Robertson, F. C., Hubbard, K. E. & Webb, A. A. Photosynthetic entrainment of the Arabidopsis thaliana circadian clock. Nature 502, 689–92 (2013).
Para, A. et al. PRR3 Is a vascular regulator of TOC1 stability in the Arabidopsis circadian clock. Plant Cell 19, 3462–3473 (2007).
Kim, S.-G., Yon, F., Gaquerel, E., Gulati, J. & Baldwin, I. T. Tissue specific diurnal rhythms of metabolites and their regulation during herbivore attack in a native tobacco, Nicotiana attenuata. PLoS ONE 6, e26214 (2011).
Endo, M. Tissue-specific circadian clocks in plants. Curr. Opin. Plant Biol. 29, 44–49 (2015).
Storch, K.-F. et al. Extensive and divergent circadian gene expression in liver and heart. Nature 417, 78–83 (2002).
Panda, S. et al. Coordinated transcription of key pathways in the mouse by the circadian clock. Cell 109, 307–320 (2002).
Zhang, R., Lahens, N. F., Ballance, H. I., Hughes, M. E. & Hogenesch, J. B. A circadian gene expression atlas in mammals: implications for biology and medicine. Proc. Natl. Acad. Sci. USA 111, 16219–16224 (2014).
Ruben, M. D. et al. A database of tissue-specific rhythmically expressed human genes has potential applications in circadian medicine. Sci Transl Med 10, (2018).
Yeung, J. & Naef, F. Rhythms of the genome: circadian dynamics from chromatin topology, tissue-specific gene expression, to behavior. Trends in Genetics 34, 915–926 (2018).
Yeung, J. et al. Transcription factor activity rhythms and tissue-specific chromatin interactions explain circadian gene expression across organs. Genome Res. 28, 182–191 (2018).
Smieszek, S. P., Yang, H., Paccanaro, A. & Devlin, P. F. Progressive promoter element combinations classify conserved orthogonal plant circadian gene expression modules. J R Soc Interface 11, (2014).
Lu, S. X. et al. The Jumonji C Domain-Containing Protein JMJ30 regulates period length in the Arabidopsis circadian clock. Plant Physiology 155, 906–915 (2011).
Malapeira, J., Khaitova, L. C. & Mas, P. Ordered changes in histone modifications at the core of the Arabidopsis circadian clock. Proceedings of the National Academy of Sciences of the United States of America 109, 21540–5 (2012).
Lee, H. G., Lee, K., Jang, K. & Seo, P. J. Circadian expression profiles of chromatin remodeling factor genes in Arabidopsis. J. Plant Res. 128, 187–199 (2015).
Yang, P., Wang, J., Huang, F.-Y., Yang, S. & Wu, K. The plant circadian clock and chromatin modifications. Genes (Basel) 9, (2018).
Long, H. K., Prescott, S. L. & Wysocka, J. Ever-changing landscapes: transcriptional enhancers in development and evolution. Cell 167, 1170–1187 (2016).
Aguilar-Arnal, L. et al. Cycles in spatial and temporal chromosomal organization driven by the circadian clock. Nature Structural & Molecular Biology 20, 1206–1213 (2013).
Kim, Y. H. et al. Rev-erbα dynamically modulates chromatin looping to control circadian gene transcription. Science 359, 1274–1277 (2018).
Mermet, J. et al. Clock-dependent chromatin topology modulates circadian transcription and behavior. Genes Dev. 32, 347–358 (2018).
Schubert, V., Lermontova, I. & Schubert, I. The Arabidopsis CAP-D proteins are required for correct chromatin organisation, growth and fertility. Chromosoma 122, 517–533 (2013).
Liu, C., Cheng, Y.-J., Wang, J.-W. & Weigel, D. Prominent topologically associated domains differentiate global chromatin packing in rice from Arabidopsis. Nat Plants 3, 742–748 (2017).
Hoyle, N. P. et al. Circadian actin dynamics drive rhythmic fibroblast mobilization during wound healing. Sci Transl Med 9, (2017).
Sinturel, F. et al. Diurnal oscillations in liver Mass and cell size accompany ribosome assembly cycles. Cell 169, 651–663.e14 (2017).
Xu, G. Sensing and transport of nutrients in plants. Semin. Cell Dev. Biol. 74, 78–79 (2018).
López-Salmerón, V., Cho, H., Tonn, N. & Greb, T. The phloem as a mediator of plant growth plasticity. Curr. Biol. 29, R173–R181 (2019).
Osorio, S., Ruan, Y.-L. & Fernie, A. R. An update on source-to-sink carbon partitioning in tomato. Front. Plant Sci. 5, (2014).
Inoue, K., Araki, T. & Endo, M. Oscillator networks with tissue-specific circadian clocks in plants. Seminars in Cell & Developmental Biology 83, 78–85 (2018).
Souza, A. P. D., Grandis, A., Arenque-Musa, B. C. & Buckeridge, M. S. Diurnal variation in gas exchange and nonstructural carbohydrates throughout sugarcane development. Functional Plant Biol. 45, 865–876 (2018).
Chandra, A., Jain, R. & Solomon, S. Complexities of invertases controlling sucrose accumulation and retention in sugarcane. Current Science 102, 857–866 (2012).
Lingle, S. E. Evidence for the Uptake of Sucrose Intact into Sugarcane Internodes. Plant Physiol 90, 6–8 (1989).
Moore, P. H. Temporal and Spatial Regulation of Sucrose Accumulation in the Sugarcane Stem. Functional Plant Biol. 22, 661–679 (1995).
Buczynski, S. R., Thom, M., Chourey, P. & Maretzki, A. Tissue Distribution and Characterization of Sucrose Synthase Isozymes in Sugarcane. Journal of Plant Physiology 142, 641–646 (1993).
Thirugnanasambandam, P. P. et al. Analysis of the diversity and tissue specificity of sucrose synthase genes in the long read transcriptome of sugarcane. BMC Plant Biology 19, 160 (2019).
Gu, Z., Gu, L., Eils, R., Schlesner, M. & Brors, B. circlize Implements and enhances circular visualization in R. Bioinformatics 30, 2811–2812 (2014).
Gu, Z., Eils, R. & Schlesner, M. Complex heatmaps reveal patterns and correlations in multidimensional genomic data. Bioinformatics 32, 2847–2849 (2016).
Acknowledgements
This work was supported by the São Paulo Research Foundation (FAPESP) (grant nos. 11/00818-8 and 15/06260-0; BIOEN Program) and by the Serrapilheira Institute (grant no. Serra-1708-16001). L.L.B.D, C. A. L., and N. O. L. were supported by FAPESP scholarships (grants 11/08897-4, 13/05301-9, and 16/06740-4, respectively). F.M.A.J was supported by CAPES scholarships (Finance Code 001).
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Conceptualization, L.L.B.D., M.S.C., G.M.S., C.T.H.; Methodology, L.L.B.D., C.T.H.; Software, M.Y.N., C.T.H.; Validation, L.L.B.D., N.O.L., F.M.A.J.; Investigation, L.L.B.D., N.O.L., C.A.L., C.T.H.; Resources, M.S.C., G.M.S.; Data Curation, M.Y.N., C.T.H.; Writing – Original Draft, L.L.B.D., C.T.H.; Writing – Review & Editing, L.L.B.D., C.A.L., C.T.H.; Visualization; C. T. H.; Project Administration, C. T. H.; Funding Acquisition, C.T.H.
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Dantas, L.L.d.B., Almeida-Jesus, F.M., de Lima, N.O. et al. Rhythms of Transcription in Field-Grown Sugarcane Are Highly Organ Specific. Sci Rep 10, 6565 (2020). https://doi.org/10.1038/s41598-020-63440-7
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DOI: https://doi.org/10.1038/s41598-020-63440-7
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