Abstract
Suspicion of myeloproliferative neoplasms (MPNs) and especially essential thrombocythemia (ET) in primary care is often based solely on blood counts, with patients referred to a haematologist without a thorough evaluation. We retrospectively assessed the role of calreticulin gene (CALR) mutations in the diagnosis of MPN in this population. We studied CALR mutations in 524 JAK2 V617F-negative patients with suspected MPN. Uncommon CALR mutations were confirmed by Sanger sequencing and searched for in the COSMIC or HGMD database. Mutations were defined as frameshift or non-frameshift mutations. CALR mutations were detected in 23 patients (23/524 = 4.4%). Four mutations detected in our study were newly identified mutations. Non-frameshift mutations were detected in two patients. Most patients (380/524 = 72.5%) were diagnosed with secondary conditions leading to blood count abnormalities such as iron deficiency, inflammatory and infectious diseases, malignancy and hyposplenism. Nine patients (9/23 = 39%) were retrospectively diagnosed with ET based on CALR mutation confirmation. Two patients with non-frameshift CALR mutations were diagnosed with reactive thrombocytosis and MPN unclassifiable, respectively. Our study showed that CALR mutations are important, non-invasive diagnostic indicators of ET and can aid in its diagnosis. Moreover, the type of CALR mutation must be accurately defined, as non-frameshift mutations may not be associated with ET. Finally, CALR mutation detection should be reserved for patients with high suspicion of clonal haematological disease.
Similar content being viewed by others
Introduction
In 2013, mutations in the calreticulin gene (CALR) were identified in two Philadelphia chromosome (Ph)-negative myeloproliferative neoplasms (MPNs), essential thrombocythemia (ET) and primary myelofibrosis (PMF)1,2. In addition, CALR mutations have been detected rarely in chronic myelomonocytic leukaemia (CMML)1, myelodysplastic syndrome/myeloproliferative neoplasm (MDS/MPN)3, and a few myelodysplastic syndrome (MDS) patients, mainly with refractory anaemia with ring sideroblasts, refractory anaemia, and refractory anaemia with excess blasts4. The presence of a CALR mutation has been described as an exceptional finding in cases of polycythaemia vera (PV) with an unknown pathogenic role5. Since its discovery, more than 50 mutations in the CALR gene have been detected as occurring in exon 9, inducing a + 1 (−1 + 2) frameshift. Only mutations leading to this +1 frameshift are considered to be pathogenic. Other mutations are usually germline variants of CALR and are not known to be pathogenic6.
Calreticulin (CALR) is a chaperone protein involved in many cellular processes in the cytoplasm and in the endoplasmic reticulum (ER). In the ER, it acts as a calcium binding protein7. It has three domains with oncogenic properties reflected by the C terminal domain8. The C terminal domain of mutant CALR is devoid of the KDEL motif, which is important for protein retention in the ER. The mutated C terminal domain contains a new amino acid sequence that bears positive charges2. The exact mechanism underlying the MPN phenotype in patients with mutant CALR remains unclear. Most important is the specific activation of the thrombopoietin receptor (TpoR/MPL) and uncontrolled activation of the JAK2/STAT pathway in the MAP kinase pathway via TpoR9.
The two most frequent CALR mutations are a 52 bp deletion (p.L367fs*46), also called type 1, and a 5 bp insertion (p.K385fs*47), also called type 2. According to these structural changes, the other mutations have been classified as type 1–like or type 2–like using algorithms based on the preservation of an α helix close to wild type CALR10.
The diagnostic value of CALR mutation confirmation has been defined only recently by including CALR mutations in the diagnostic criteria for ET/PMF in the 2016 revision to the World Health Organization (WHO) classification of myeloid neoplasms and acute leukaemia11.
Suspicion for MPN in primary care is often based solely on complete blood cell count, with patients referred to a haematologist without a thorough history, examination or laboratory testing.
The aim of our study was to retrospectively confirm the diagnostic importance of CALR mutations in JAK2 V617F-negative patients with suspected MPN based on clinical and/or laboratory parameters who were referred to the Department of Haematology by general practitioners. Furthermore, our goal was to determine possible novel CALR mutations and their clinical effects, including thrombo-haemorrhagic complications in CALR-positive patients.
Patients and Methods
Study design
CALR mutations were studied in JAK2 V617F-negative patients with suspected MPN based on clinical and/or laboratory parameters (listed in Table 1) who were referred by general practitioners and then examined at the Department of Haematology, University Medical Centre Ljubljana, between April 7, 2011 and September 13, 2016 (inclusion and exclusion criteria are listed in Table 2). The study was retrospective and nonrandomized and was approved by the National Medical Ethics Committee, Ministry of Health, Republic of Slovenia, on November 14, 2017. All experiments were performed in accordance with relevant guidelines and regulations, and informed consent was obtained from all the participants.
We collected history, clinical and laboratory data from our institutional database. The following data were collected from our institutional database: age at the date of first examination, gender, haemoglobin level, leukocyte blood count, and platelet count, the presence of splenomegaly or unexplained arterial/venous thrombosis and haematological diagnosis defined at the Department of Haematology. CALR-positive patients were re-diagnosed after CALR mutation confirmation based on available clinical and laboratory data. ET was diagnosed by the 2008 WHO criteria12. In patients without bone marrow samples, modified WHO criteria were used13. Other MPN subtypes were diagnosed according to the 2008 WHO criteria12. CALR-positive patients were screened for thrombo-haemorrhagic complications according to the available data from follow-up visits at the Department of Haematology.
Molecular-genetic testing
CALR mutations were analysed in patients’ neutrophil granulocytes. Granulocytes were isolated from venous blood samples by Ficoll density gradient centrifugation followed by a red blood cell lysis procedure14. DNA was isolated from granulocytes by a QIAamp DNA Mini Kit from Qiagen (USA).
The detection of CALR exon 9 mutations was performed with fluorescence-based quantitative real-time PCR (qPCR) and post-qPCR analysis with the High Resolution Melting-Curve Analysis (HRM) method. A MeltDoctor HRM MasterMix (Applied Biosystems, Thermo Fischer Scientific, USA) and primer sets published previously2 were used for the qPCR and HRM analysis. Both were performed according to the manufacturer’s instructions on an ABI PRISM ViiA 7 Real-Time PCR system (Applied Biosystems, Thermo Fisher Scientific). Specifically, samples of 20 ng DNA were amplified by qPCR using the following thermal cycling protocol: 95 °C, 10 minutes for 1 cycle, followed by 50 cycles of 95 °C for 10 seconds and 62.5 °C for 60 seconds. Melt curve/dissociation stage was carried out immediately after qPCR according to the manufacturer’s instructions (Applied Biosystems, Thermo Fischer Scientific). We included two positive controls (NM_004343.3 (CALR): c.1099_1150del52, p.(Leu367Thrfs*46) (52 bp deletion or type 1 mutation) and NM_004343.3 (CALR): c.1154_1155insTTGTC, p.(Lys385Asnfs*47) (5 bp insertion or type 2 mutation)), as well as wild-type and non-template controls, in the qPCR and HRM setup. qPCR products were visualized on a 4% agarose E-gel by the E-Gel Precast Agarose Electrophoresis System (Invitrogen, Thermo Fischer Scientific, USA). The most frequent mutations, the 52 bp deletion (type 1 mutation) and the 5 bp insertion (type 2 mutation), were confirmed by a typical HRM melt curve and band pattern on the E-gel. An unusual HRM melt curve and/or band pattern on the E-gel was indicative of a different genetic variant, which was confirmed by Sanger sequencing according to the recommended protocol from Applied Biosystems, Thermo Fischer Scientific Company. Mutations were defined by type as type 1, type 2, type 1-like or type 2-like by the AGADIR-derived predicted helix propensity scale (AGADIR score)15,16,17.
The Catalogue of Somatic Mutations in Cancer (COSMIC)18 and The Human Gene Mutation Database (HGMD)19 were used to determine the impact of new genetic variants of the CALR gene.
Statistical analysis
Numerical variables are summarized by their median and range and categorical variables by count and relative frequency (%). Statistical analysis was performed using the JASP 0.9.2.0 statistical program.
Results
Patient demographics
A total of 524 patients were screened for the presence of CALR mutations. Of these patients, 292 were female and 232 were male. The median age at diagnosis was 55 years. Demographic and laboratory parameters of patients suspected of MPN are presented in Table 3.
At the Department of Haematology, most patients (380/524 = 72.5%) were identified with secondary, non-clonal conditions, resulting in erythrocytosis, leucocytosis or thrombocytosis. The most common secondary conditions or factors were iron deficiency, infectious and inflammatory diseases, hyposplenism, malignancy, smoking, recent surgery, use of corticosteroids and chronic hypoxia. Diagnosis was defined after the examination and diagnostic workup at the Department of Haematology. Twenty-six patients (26/524 = 4.96%) were diagnosed with different types of MPN according to the 2008 WHO criteria12. Two patients (2/524 = 0.38%) were diagnosed with chronic myelomonocytic leukaemia (CMML). Thirty-two patients (32/524 = 6.1%) were suspected of having clonal ET but were not diagnosed at the time of examination. The list of diagnoses in MPN-suspected patients is presented in Supplementary Table S1.
CALR mutation detection
CALR mutations were detected in 23 patients (23/524 = 4.4%). The types of CALR mutations found in our cohort of patients are listed in Table 4. Four mutations that were detected in our study were newly identified mutations that had not yet been published in the COSMIC or HGMD database. They are marked in bold in Table 4. Two CALR mutations detected in our study are non-frameshift mutations and are underlined in Table 4.
Sixteen mutations (16/23 = 69.6%) that were found in the COSMIC database were confirmed somatic. All other mutations were of unidentified status.
The most common type of CALR mutation was a 52 bp deletion (type 1 mutation) that was diagnosed in fourteen patients (14/23 = 60.9%). A type 2 mutation (5 bp insertion) was present in two patients (2/23 = 8.7%). Other CALR mutations were present in 7 patients (7/23 = 30.4%) and were sub-classified by the AGADIR‐derived predicted helix propensity score as described in the literature17. The AGADIR score was 17.50 for type 1, 40.02 for type 2 and 33.62 for wild-type CALR. CALR variants with an AGADIR scale of 26% or less were classified as type 1-like and CALR variants with an AGADIR scale of 30% or more as type 2-like. According to the AGADIR scale, five patients were diagnosed with type 2-like mutations (Table 5). Two patients did not have a frameshift mutation and were not sub-classified as type 1- or type 2-like.
CALR-positive patients
The most common diagnosis in CALR-positive patients was ET, which was present in 16 patients (16/23 = 69.6%). Nine patients (9/23 = 39%) were retrospectively diagnosed with ET according to modified WHO ET criteria based on clinical and laboratory findings and CALR mutation confirmation. In other patients, the CALR mutation did not affect haematological diagnosis. The list of haematological diagnoses in the CALR-positive patients is presented in Table 6.
Seven CALR-positive patients (7/23 = 30%) developed thrombo-haemorrhagic complications. Five patients (5/23 = 21.7%) developed thrombotic complications, and two patients (2/23 = 8.7%) developed haemorrhagic complications. Thrombo-haemorrhagic complications occurred mostly in patients diagnosed with ET (6/7 = 85.7%), and one complication occurred in a patient diagnosed with PMF. Patients with thrombo-haemorrhagic complications were mostly diagnosed with type 1 mutation (5/7 = 71.4%), and two patients were diagnosed with type 2 and type 2-like mutations. The median follow-up time was 693 days (range: 196–1270 days). A list of complications, haematological diagnoses and the times of complication is presented in Table 7.
Discussion
In our study, we retrospectively detected the presence of CALR mutations in a cohort of 524 JAK2 V617F-negative patients who presented with clinical and/or laboratory suspicion of MPN with the main goal of determining the diagnostic value of this detection. During the observed period, CALR mutations had not been routinely investigated in patients suspected of MPN in our centre, as they have only recently been discovered1,2. In contrast, the JAK2 V617F mutation has already been analysed routinely since its discovery in 200520. Therefore, JAK2 V617F-positive patients were excluded from the study. An MPL mutation is a relatively rare finding in MPN patients and is mostly present in patients with either ET or PMF21. In our laboratory, MPL testing was included in the diagnostic algorithm in patients with suspected MPN only recently and is carried out only after excluding JAK2 V617F and CALR mutations. Therefore, MPL status was not defined in all our patients at the time of the first examination.
Our centre is a university medical hospital serving an area with approximately 1.000.000 inhabitants and is a referral centre for haematological malignancies. All patients referred to the haematology department were first evaluated by general practitioners without a detailed knowledge in the field of haematological malignancies. As presented in Table 3 only a proportion of patients referred to the Department of Haematology had pathological clinical and/or laboratory parameters suspicious of MPN at the time of first examination. Therefore, most patients who were referred to the Department of Haematology were individuals without a haematological malignancy, as we determined that 380 patients (380/524 = 72.5%) were MPN free. These patients were diagnosed with secondary changes in the peripheral blood content caused by different conditions, such as iron deficiency, infectious and inflammatory diseases, hyposplenism, malignancy, smoking, recent surgery, use of corticosteroids and chronic hypoxia. Accordingly, the number of CALR-positive patients in our cohort was low. We identified 4.4% of patients suspected for MPN as CALR positive, most of whom were diagnosed with either ET or PMF. Most similar studies analysed the number of CALR-positive patients with confirmed MPN; therefore, the number of CALR-positive patients was much higher, ranging from 12 to more than 20%22,23,24,25,26. Based on our data, it would be beneficial to improve the diagnostic approach to patients with increased levels of one or more blood cell lineages, as in most cases, this is not caused by haematological malignancy. Patients should be routinely screened for the most common causes of secondary erythrocytosis, thrombocytosis, and leucocytosis, and blood count should be repeated at least once at follow-up visits before referral to the Department of Haematology. Molecular genetic testing should be reserved for patients with high suspicion of clonal haematological disease and not performed in all patients referred to the Department of Haematology.
CALR mutations are commonly identified in JAK2 V617F-negative patients with ET1,2. In our centre, only a minority of CALR-positive patients (7/23 = 30%) were diagnosed with ET according to the 2008 WHO criteria at the time of the first examination. The main reason for this was the relative reservation towards bone marrow examination in patients with moderate thrombocytosis and a low risk of thrombotic complications. However, this means we could have underestimated the number of patients with clonal thrombocytosis. By confirming the presence of CALR mutations, we were able to retrospectively diagnose 9 patients with ET according to the modified WHO criteria for ET13. Although all of these patients had follow-up at our department due to high suspicion of clonal thrombocytosis, it is of great prognostic and therapeutic benefit to be able to confirm the diagnosis of ET by a non-invasive procedure such as molecular-genetic testing. CALR mutations are therefore important diagnostic hallmarks for ET as has also been confirmed recently in the literature27,28.
All patients with PMF were diagnosed according to the WHO criteria at the time of the examination at the Department of Haematology. CALR mutation identification retrospectively confirmed the diagnosis but had no direct diagnostic or therapeutic impact.
CMML is a subtype of MDSs/MPNs and not strictly MPNs12. CALR mutations in patients with CMML are extremely rare and do not play an important role in its pathogenesis. A group study by Zamora et al. showed that only 1 out of 174 patients with CMML presented with a CALR mutation29. The patient in our study who was diagnosed with CMML type one and later progressed to myelofibrosis might have been misdiagnosed at the time of the first examination, as PMF and CMML share many common features, including monocytosis and bone marrow fibrosis. In a recent study by Hu et al., a more accurate analysis enabled many patients diagnosed with CMML to be reclassified as having PMF, showing that a more in-depth analysis should be performed to make an accurate diagnosis, especially in patients with molecular biomarkers typical for ET/PMF11,30.
More than 50 frameshift CALR mutations have been described, and all are located in exon 9 of the CALR gene. There are two main types of CALR mutations: type 1 (a 52-bp deletion; p.L367fs*46) and type 2 (a 5-bp TTGTC insertion; p.K385fs*47). Based on their molecular characteristics, other mutations can be grouped as type 1- like and type 2- like31. In our study, the most common type of CALR mutation was type 1, which is consistent with the data from the literature2,16,32. Mutations that are neither type 1 nor type 2 should be sub-classified as type 1-like or type 2-like, as this may have an impact on clinical phenotype and, in the case of PMF, even survival17. Type 1-like and type 2-like mutations in our study were defined by using AGADIR15, a statistical approximation algorithm that calculates helix propensity for the 31 unique amino acid sequences that are altered by CALR mutations16. This algorithm has been used in similar studies and represents an important tool in CALR mutation sub-classification16,17. It seems extremely important to properly define the type of CALR mutation, as this also has a diagnostic impact. It is known that only mutations leading to a + 1 (−1 + 2) frameshift of the reading frame are pathogenic6. Other CALR mutations can be germline variants of CALR with an unknown clinical significance. In our study, non-frameshift CALR mutations were detected in two patients. One of these patients was diagnosed with reactive thrombocytosis at the time of examination at the Department of Haematology and was retrospectively defined as CALR-positive. A detailed analysis confirmed a missense CALR mutation that results in the substitution of glutamic acid with aspartic acid at amino acid position 398. The same mutation was discovered in a patient with WHO-defined chronic neutrophilic leukaemia (CNL) in a study by Lasho et al. His study concluded that CALR mutations that do not result in a generation of a distinct C-terminus are suggestive of a different pathogenic mechanism that might be yet unknown33. In our study, we assumed that our patient did not develop a CALR mutation that would imply a clonal haematological disease. According to the patient’s history, clinical examination and laboratory findings, it was mostly suggestive of reactive thrombocytosis. At the time of writing this article, the patient was in good health without any possible complications associated with clonal haematological disease.
Another patient with a non-frameshift CALR mutation was diagnosed with MPN-U. This patient carried a germline in-frame deletion in the CALR gene (NM_004343.3 (CALR):c.1142_1144delAGG; p.(Glu381del)), which had already been recognized in a symptomatic patient with MPN34. Although the KDEL motif was preserved, the deletion of one amino acid (p.(Glu381del)) led to the alteration of the secondary structure of the protein as well as the three-dimensional structure, which led to the conclusion of the pathogenic nature of this in-frame deletion23,34,35. Our patient with MPN-U was unfortunately lost to follow-up, and an exact haematological diagnosis or possible complications could not be defined. However, laboratory findings were suggestive of a clonal haematological disease.
In our study, we discovered four novel CALR mutations in exon 9 that, to our knowledge, have not yet been registered in the COSMIC (https://cancer.sanger.ac.uk/cosmic) or HGMD (http://www.hgmd.cf.ac.uk) database. The COSMIC database is the world’s largest expert-curated database of somatic mutations in human cancer. It describes over 4 million coding mutations36. The HGMD database represents an attempt to collate all known (published) gene lesions responsible for human inherited diseases19. The novel mutations that were defined in our study are NM_004343.3 (CALR):c.1127_1145del19, p.(Arg376Glnfs*48), NM_004343.3 (CALR):c.1154_1154delAinsGTTGTC, p.(Lys385Serfs*47), NM_004343.3 (CALR):c.1154_1154delAinsTTTATC, p.(Lys385Ilefs*47), and NM_004343.3 (CALR):c.1132_1153del22, p.(Glu378Argfs*45). All these mutations were type 2-like mutations.
ET and PMF are associated with an increased risk of thrombotic and thromboembolic events, which represent important causes of morbidity and mortality37. Reducing the risk of thrombotic and thromboembolic complications is one of the most important goals of treatment, especially in patients with ET38. These patients are also at higher risk of bleeding complications, which may be related to complications of treatment or acquired von Willebrand syndrome (AVWS) due to extreme thrombocytosis (platelets > 1000 × 109/L)28,39,40. The risk of thrombosis in patients with ET exceeds 20%41. In a Swedish study, 35% of patients with ET developed vascular complications42. In PMF, thrombotic events are about as common as in ET43. The prevalence for thrombotic complications in patients with PMF ranges from 7 to 30%44,45,46. In our study, the prevalence of thrombotic complications in CALR-positive patients was 30%. However, three patients had developed a thrombotic complication more than 10 years before MPN diagnosis was suspected. The true prevalence of thrombotic complications in our study was therefore lower. One patient developed vitreous haemorrhage which was a result of inadequate anticoagulation therapy with warfarin. Neither of the two patients with a bleeding complication had extreme thrombocytosis at the time of complication. Compared to JAK2 V617F and MPL mutations, CALR is a favourable mutation and is associated with a lower incidence of thrombotic events1,2,47. Most CALR-positive patients who developed thrombo-haemorrhagic complications were diagnosed with type 1 mutations (71.4%). As already shown, patients with type 1-like mutations had a higher risk of thrombosis compared to patients with type 2-like mutations10.
CALR mutations are currently known to be one of the three major mutation types, in addition to JAK2 V617F and the MPL mutation, in patients with ET or PMF. However, there were still 10–15% of patients with ET or PMF with an unknown molecular genetic marker underlying the disease. These patients are termed ‘triple negative’48. In these patients, novel molecular biomarkers have been searched by sequencing coding exons in myeloid cancer genes, which has shown promising results. This may provide a personalized approach to diagnosis in patients with MPN49.
CALR mutations in MPN patients are also under investigation for their therapeutic potential50,51,52. CALR exon 9 mutations could be targets for cancer immune therapy, as they have been shown to act as immunogenic neo-antigens51. In the treatment of MPN, it could be beneficial to combine CALR vaccines with immunomodulatory treatments53 such as interferon-alpha (IFN-α)54 or programmed death 1 ligand (PD-L1)55 as a combinatorial cancer vaccination53. CALR mutations, in addition to being important diagnostic and prognostic markers in patients with MPN, could become an important therapeutic target in a subgroup of patients with MPN in the future.
Conclusion
As a non-invasive test, detection of CALR mutations is important in the diagnosis of ET, especially in cases where bone marrow examination is not available or unwarranted. The type of CALR mutation must be accurately defined, as some CALR mutations may not be associated with ET. CALR mutation detection should be reserved for patients with high suspicion of clonal haematological disease. In the future, CALR exon 9 mutations, as immunogenic neo-antigens, could be targets for cancer immune therapy.
Data availability
The datasets generated and analysed during the current study are available from the corresponding author on reasonable request.
References
Nangalia, J. et al. Somatic CALR mutations in myeloproliferative neoplasms with nonmutated JAK2. N Engl J Med 369, 2391–2405, https://doi.org/10.1056/NEJMoa1312542 (2013).
Klampfl, T. et al. Somatic mutations of calreticulin in myeloproliferative neoplasms. N Engl J Med 369, 2379–2390, https://doi.org/10.1056/NEJMoa1311347 (2013).
Heuser, M. et al. Low frequency of calreticulin mutations in MDS patients. Leukemia 28, 1933–1934, https://doi.org/10.1038/leu.2014.165 (2014).
Hou, H. A., Kuo, Y. Y., Chou, W. C., Chen, P. H. & Tien, H. F. Calreticulin mutation was rarely detected in patients with myelodysplastic syndrome. Leukemia 28, 1555–1557, https://doi.org/10.1038/leu.2014.71 (2014).
Broseus, J., Park, J. H., Carillo, S., Hermouet, S. & Girodon, F. Presence of calreticulin mutations in JAK2-negative polycythemia vera. Blood 124, 3964–3966, https://doi.org/10.1182/blood-2014-06-583161 (2014).
Vainchenker, W. & Kralovics, R. Genetic basis and molecular pathophysiology of classical myeloproliferative neoplasms. Blood 129, 667–679, https://doi.org/10.1182/blood-2016-10-695940 (2017).
Raghavan, M., Wijeyesakere, S. J., Peters, L. R. & Del Cid, N. Calreticulin in the immune system: ins and outs. Trends in immunology 34, 13–21, https://doi.org/10.1016/j.it.2012.08.002 (2013).
Marty, C. et al. Calreticulin mutants in mice induce an MPL-dependent thrombocytosis with frequent progression to myelofibrosis. Blood 127, 1317–1324, https://doi.org/10.1182/blood-2015-11-679571 (2016).
Chachoua, I. et al. Thrombopoietin receptor activation by myeloproliferative neoplasm associated calreticulin mutants. Blood 127, 1325–1335, https://doi.org/10.1182/blood-2015-11-681932 (2016).
Pietra, D. et al. Differential clinical effects of different mutation subtypes in CALR-mutant myeloproliferative neoplasms. Leukemia 30, 431–438, https://doi.org/10.1038/leu.2015.277 (2016).
Arber, D. A. et al. The 2016 revision to the World Health Organization classification of myeloid neoplasms and acute leukemia. Blood 127, 2391–2405, https://doi.org/10.1182/blood-2016-03-643544 (2016).
Vardiman, J. W. et al. The 2008 revision of the World Health Organization (WHO) classification of myeloid neoplasms and acute leukemia: rationale and important changes. Blood 114, 937–951, https://doi.org/10.1182/blood-2009-03-209262 (2009).
Harrison, C. N. et al. Guideline for investigation and management of adults and children presenting with a thrombocytosis. British journal of haematology 149, 352–375, https://doi.org/10.1111/j.1365-2141.2010.08122.x (2010).
Pajič, T., Kovačič, L., Mlakar, U. The thrombopoietin receptor W515L and W515K mutations detection in patients with essential thrombocythemia. Zdrav Vestn 81 (2012).
Munoz, V. & Serrano, L. Development of the multiple sequence approximation within the AGADIR model of alpha-helix formation: comparison with Zimm-Bragg and Lifson-Roig formalisms. Biopolymers 41, 495–509, 10.1002/(sici)1097-0282(19970415)41:5<495::Aid-bip2>3.0.Co;2-h (1997).
Tefferi, A. et al. The prognostic advantage of calreticulin mutations in myelofibrosis might be confined to type 1 or type 1-like CALR variants. Blood 124, 2465–2466, https://doi.org/10.1182/blood-2014-07-588426 (2014).
Lasho, T. L., Finke, C. M., Tischer, A., Pardanani, A. & Tefferi, A. Mayo CALR mutation type classification guide using alpha helix propensity. American journal of hematology 93, E128–e129, https://doi.org/10.1002/ajh.25065 (2018).
Forbes, S. A. et al. COSMIC: somatic cancer genetics at high-resolution. Nucleic acids research 45, D777–d783, https://doi.org/10.1093/nar/gkw1121 (2017).
Stenson, P. D. et al. The Human Gene Mutation Database: towards a comprehensive repository of inherited mutation data for medical research, genetic diagnosis and next-generation sequencing studies. Human genetics 136, 665–677, https://doi.org/10.1007/s00439-017-1779-6 (2017).
Kralovics, R. et al. A gain-of-function mutation of JAK2 in myeloproliferative disorders. N Engl J Med 352, 1779–1790, https://doi.org/10.1056/NEJMoa051113 (2005).
Pikman, Y. et al. MPLW515L is a novel somatic activating mutation in myelofibrosis with myeloid metaplasia. PLoS medicine 3, e270, https://doi.org/10.1371/journal.pmed.0030270 (2006).
Tefferi, A. et al. Type 1 versus Type 2 calreticulin mutations in essential thrombocythemia: a collaborative study of 1027 patients. American journal of hematology 89, E121–124, https://doi.org/10.1002/ajh.23743 (2014).
Kim, S. Y. et al. CALR, JAK2, and MPL mutation profiles in patients with four different subtypes of myeloproliferative neoplasms: primary myelofibrosis, essential thrombocythemia, polycythemia vera, and myeloproliferative neoplasm, unclassifiable. American journal of clinical pathology 143, 635–644, https://doi.org/10.1309/ajcpuaac16liwzmm (2015).
Rabade, N. et al. Molecular genetics of BCR-ABL1 negative myeloproliferative neoplasms in India. Indian journal of pathology & microbiology 61, 209–213, https://doi.org/10.4103/ijpm.Ijpm_223_17 (2018).
Ojeda, M. J. et al. CALR, JAK2 and MPL mutation status in Argentinean patients with BCR-ABL1- negative myeloproliferative neoplasms. Hematology (Amsterdam, Netherlands) 23, 208–211, https://doi.org/10.1080/10245332.2017.1385891 (2018).
Wang, J. et al. JAK2, MPL, and CALR mutations in Chinese Han patients with essential thrombocythemia. Hematology (Amsterdam, Netherlands) 22, 145–148, https://doi.org/10.1080/10245332.2016.1252003 (2017).
Geyer, J. T. & Orazi, A. Myeloproliferative neoplasms (BCR-ABL1 negative) and myelodysplastic/myeloproliferative neoplasms: current diagnostic principles and upcoming updates. International journal of laboratory hematology 38(Suppl 1), 12–19, https://doi.org/10.1111/ijlh.12509 (2016).
Tefferi, A. & Barbui, T. Polycythemia vera and essential thrombocythemia: 2017 update on diagnosis, risk-stratification, and management. American journal of hematology 92, 94–108, https://doi.org/10.1002/ajh.24607 (2017).
Zamora, L. et al. Calreticulin mutations are not present in patients with myeloproliferative chronic myelomonocytic leukemia. Annals of hematology 94, 869–871, https://doi.org/10.1007/s00277-014-2262-2 (2015).
Hu, Z. et al. Utility of JAK2 V617F allelic burden in distinguishing chronic myelomonocytic Leukemia from Primary myelofibrosis with monocytosis. Human pathology 85, 290–298, https://doi.org/10.1016/j.humpath.2018.10.026 (2019).
Guglielmelli, P. et al. Validation of the differential prognostic impact of type 1/type 1-like versus type 2/type 2-like CALR mutations in myelofibrosis. Blood cancer journal 5, e360, https://doi.org/10.1038/bcj.2015.90 (2015).
Rumi, E. et al. JAK2 or CALR mutation status defines subtypes of essential thrombocythemia with substantially different clinical course and outcomes. Blood 123, 1544–1551, https://doi.org/10.1182/blood-2013-11-539098 (2014).
Lasho, T. L., Elliott, M. A., Pardanani, A. & Tefferi, A. CALR mutation studies in chronic neutrophilic leukemia. American journal of hematology 89, 450, https://doi.org/10.1002/ajh.23665 (2014).
Smaili, W. et al. CALR gene mutational profile in myeloproliferative neoplasms with non-mutated JAK2 in Moroccan patients: A case series and germline in-frame deletion. Current research in translational medicine 65, 15–19, https://doi.org/10.1016/j.retram.2016.08.002 (2017).
Lin, Y. et al. The Prevalence of JAK2, MPL, and CALR Mutations in Chinese Patients With BCR-ABL1-Negative Myeloproliferative Neoplasms. American journal of clinical pathology 144, 165–171, https://doi.org/10.1309/ajcpalp51xdixddv (2015).
Forbes, S. A. et al. COSMIC: High-Resolution Cancer Genetics Using the Catalogue of Somatic Mutations in Cancer. Current protocols in human genetics 91, 10.11.11–10.11.37, https://doi.org/10.1002/cphg.21 (2016).
Barbui, T., Finazzi, G. & Falanga, A. Myeloproliferative neoplasms and thrombosis. Blood 122, 2176–2184, https://doi.org/10.1182/blood-2013-03-460154 (2013).
Tefferi, A. & Barbui, T. Polycythemia vera and essential thrombocythemia: 2019 update on diagnosis, risk-stratification and management. American journal of hematology 94, 133–143, https://doi.org/10.1002/ajh.25303 (2019).
McMahon, B. & Stein, B. L. Thrombotic and bleeding complications in classical myeloproliferative neoplasms. Seminars in thrombosis and hemostasis 39, 101–111, https://doi.org/10.1055/s-0032-1331153 (2013).
Kaifie, A. et al. Bleeding, thrombosis, and anticoagulation in myeloproliferative neoplasms (MPN): analysis from the German SAL-MPN-registry. Journal of hematology & oncology 9, 18, https://doi.org/10.1186/s13045-016-0242-9 (2016).
Tefferi, A. & Elliott, M. Thrombosis in myeloproliferative disorders: prevalence, prognostic factors, and the role of leukocytes and JAK2V617F. Seminars in thrombosis and hemostasis 33, 313–320, https://doi.org/10.1055/s-2007-976165 (2007).
Abdulkarim, K., Samuelsson, J., Johansson, P. & Andreasson, B. Risk factors for vascular complications and treatment patterns at diagnosis of 2389 PV and ET patients: Real-world data from the Swedish MPN Registry. European journal of haematology 98, 577–583, https://doi.org/10.1111/ejh.12873 (2017).
Kc, D., Falchi, L. & Verstovsek, S. The underappreciated risk of thrombosis and bleeding in patients with myelofibrosis: a review. Annals of hematology 96, 1595–1604, https://doi.org/10.1007/s00277-017-3099-2 (2017).
Barbui, T. et al. Thrombosis in primary myelofibrosis: incidence and risk factors. Blood 115, 778–782, https://doi.org/10.1182/blood-2009-08-238956 (2010).
Cervantes, F. et al. Frequency and risk factors for thrombosis in idiopathic myelofibrosis: analysis in a series of 155 patients from a single institution. Leukemia 20, 55–60, https://doi.org/10.1038/sj.leu.2404048 (2006).
Barosi, G. et al. Evidence that prefibrotic myelofibrosis is aligned along a clinical and biological continuum featuring primary myelofibrosis. PloS one 7, e35631, https://doi.org/10.1371/journal.pone.0035631 (2012).
Rotunno, G. et al. Impact of calreticulin mutations on clinical and hematological phenotype and outcome in essential thrombocythemia. Blood 123, 1552–1555, https://doi.org/10.1182/blood-2013-11-538983 (2014).
Langabeer, S. E. Chasing down the triple-negative myeloproliferative neoplasms: Implications for molecular diagnostics. Jak-stat 5, e1248011, https://doi.org/10.1080/21623996.2016.1248011 (2016).
Grinfeld, J. et al. Classification and Personalized Prognosis in Myeloproliferative Neoplasms. N Engl J Med 379, 1416–1430, https://doi.org/10.1056/NEJMoa1716614 (2018).
Cimen Bozkus, C. et al. Immune Checkpoint Blockade Enhances Shared Neoantigen-Induced T-cell Immunity Directed against Mutated Calreticulin in Myeloproliferative Neoplasms. Cancer discovery 9, 1192–1207, https://doi.org/10.1158/2159-8290.Cd-18-1356 (2019).
Holmstrom, M. O. et al. The calreticulin (CALR) exon 9 mutations are promising targets for cancer immune therapy. Leukemia 32, 429–437, https://doi.org/10.1038/leu.2017.214 (2018).
Holmstrom, M. O., Riley, C. H., Svane, I. M., Hasselbalch, H. C. & Andersen, M. H. The CALR exon 9 mutations are shared neoantigens in patients with CALR mutant chronic myeloproliferative neoplasms. Leukemia 30, 2413–2416, https://doi.org/10.1038/leu.2016.233 (2016).
Klausen, U. et al. Novel Strategies for Peptide-Based Vaccines in Hematological Malignancies. Frontiers in immunology 9, 2264, https://doi.org/10.3389/fimmu.2018.02264 (2018).
Stauffer Larsen, T. et al. Long term molecular responses in a cohort of Danish patients with essential thrombocythemia, polycythemia vera and myelofibrosis treated with recombinant interferon alpha. Leukemia research 37, 1041–1045, https://doi.org/10.1016/j.leukres.2013.06.012 (2013).
Zak, K. M. et al. Structural Biology of the Immune Checkpoint Receptor PD-1 and Its Ligands PD-L1/PD-L2. Structure (London, England: 1993) 25, 1163–1174, https://doi.org/10.1016/j.str.2017.06.011 (2017).
Acknowledgements
The authors would like to thank all the academic experts and employees at the Specialized Haematology Laboratory, Department of Haematology, Division of Internal Medicine at the Faculty of Medicine, The University of Ljubljana and University Medical Centre Ljubljana for their scientific oversight and support throughout the study.
Author information
Authors and Affiliations
Contributions
Matjaz Sever designed the study. Tanja Belcic Mikic and Tadej Pajic collected the relevant data and performed molecular-genetic testing. Data analysis was performed by Tanja Belcic Mikic, Tadej Pajic and Matjaz Sever. Tanja Belcic Mikic drafted the manuscript. Tadej Pajic and Matjaz Sever revised the manuscript. All authors reviewed the manuscript.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Belcic Mikic, T., Pajic, T. & Sever, M. CALR mutations in a cohort of JAK2 V617F negative patients with suspected myeloproliferative neoplasms. Sci Rep 9, 19838 (2019). https://doi.org/10.1038/s41598-019-56236-x
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-019-56236-x
- Springer Nature Limited