Abstract
Forests contain one of the world’s largest carbon (C) pools and represent opportunities for cost-effective climate change mitigation through programmes such as the United Nations-led “Reducing Emissions from Deforestation and Forest Degradation” Programme (REDD). Generic estimates for the conversion of forest biomass into C stock are not sufficiently accurate for assessing the utility of harvesting forest to offset carbon dioxide emissions, currently under consideration by the REDD Programme. We examined the variation in C concentration among tree species and tree functional types (classified based on leaf morphological and phenological traits) in a subtropical forest and evaluated the effects of these variations on stand-level estimations of C stock. This study was conducted in the Paiyashan Forest State Farm and the Dashanchong Forest Park, Hunan Province, China. C concentrations differed significantly among tree species (P < 0.0001) and were significantly higher in gymnosperm than angiosperm species. Estimations of stand C stocks were similar using either functional types or species- and tissue-specific C concentrations. The use of functional type classification to estimate stand C stock is an effective tool for implementing C sequestration trade and C credit programmes and the UN-REDD Programme in subtropical forests.
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Introduction
Forests are essential to the approximately 1.6 billion people who depend on them for food, water, fuel, medicines, tradition and livelihood1. Forests accumulate over 45% of the carbon (C) in the terrestrial biosphere and account for nearly half of terrestrial net primary production2. Therefore, forests play a vital role in regulating atmospheric C concentrations and our climate by natural C sequestration. Yet, on average, thirteen million hectares of forests disappear annually, often devastating the well-being of local communities1. Deforestation and forest degradation account for over 10% of greenhouse gas emissions3, making the reduction of emissions from deforestation and the increase of forest restoration important consideration for limiting global warming to +2 °C4. The United Nations Collaborative Programme on “Reducing Emissions from Deforestation and Forest Degradation in Developing Countries” (UN-REDD Programme) was launched to reduce forest emissions and enhance C stocks in forests while contributing to national sustainable development1, 5. As part of UN-based forest C accounting protocols6, 7, accurate measures of C concentrations for diverse tree species are needed to estimate the size of C stock in forest biomass. Studies show that C concentrations vary among the tree species8,9,10. Thus, one of the main challenges for implementing the UN-REDD Programme will be accounting for the effects of these variations on forest C stock estimates.
Forest C stocks are commonly estimated by multiplying forest biomass (dry weight) with a constant conversion factor (C concentration)6, 7. One widely accepted value for this conversion factor is 50.0% by mass11, 12, though an increasing number of studies indicate that C concentration varies significantly among tree species and tissues8, 13. In one instance, the C concentrations of 5 tree species common in subtropical forests varied from 47.9% to 57.7%14. The average C concentrations for tree tissues in 10 Chinese temperate species were 49.9% for stems, 50.3% for old branches, 50.8% for new branches, 51.4% for leaves, 47.1% for fine roots and 48.8% for coarse roots8. Ignoring species- and tissue-specific variations in C concentration and using the generic value of 50% could introduce between −6.2% to 7.0% error in stand C stock estimates from forest inventory data, or a 10% error in stand biomass C stock estimates from 32 neotropical tree species8, 10, 13, 15, 16. It is recognised that species- and tissue-specific variations in C concentration need be considered to meet the accuracy required by C sequestration trade, C credits9 and the UN-REDD Programme.
Even though species-specific C concentrations are preferred when estimating stand C stock, it is impractical to obtain the data for every species in highly diverse forest ecosystems. Generalizing C concentration based on tree phylogeny (e.g. gymnosperm and angiosperm species) and functional traits may provide a realistic alternative. This possibility is supported by research showing that C concentrations vary between functional types10, 17, 18. For example, the C concentration of woody tissue is greater in gymnosperm species (50.8%) than angiosperm species (47.7%)17, likely attributable to differences in lignin chemistry19. Gymnosperm species have more highly lignified stem wood20, while angiosperm species have higher concentrations of non-structural carbohydrates21, demonstrating how broad phylogenetic differences may inform measurements of C concentration. The relationships between functional traits and C concentrations and functional types and variations in C concentrations among tree species have not been fully examined.
Plant functional traits and their associated trade-offs control a variety of terrestrial ecosystem processes – including forest C stock, a key component of the global C cycle22,23,24. Leaves assimilate C through photosynthesis and stems accumulate the greatest C stock. The relevant functional traits to C concentration in leaves22, 25 include: leaf area (LA), specific leaf area (SLA), photosynthetic capacity, leaf nitrogen and phosphorus concentrations, dark respiration rate and leaf lifespan22, 25. For stems, the functional traits of note are maximum plant height, wood density (WD), wood specific gravity, relative growth rate (RGR) and mean annual increment of biomass (MAI)8, 24. WD has proven to be inconsistently correlated with C concentration8, 10, 13, 15. For instance, Elias and Potvin found that C concentration was highly positively correlated with WD13, while Thomas and Malczewski report a non-significant negative correlation with WD for gymnosperm and a reverse trend for angiosperms10. Most researches have shown no relationship between C concentration and WD8, 15, 26. Zhang et al. found a significant negative relationship between C concentration and MAI8. Martin and Thomas found that C concentration was not correlated with RGR15. SLA is frequently used in growth analysis because it is positively correlated to RGR across species, and tends to scale negatively with C shunting to more recalcitrant C-containing secondary compounds, such as tannins or lignin27. LA is negatively related to WD28,29,30,31, but no relationship between C concentration and LA has been reported. We investigated the correlation between five functional traits (WD, MAI, RGR, SLA and LA) and C concentrations, while controlling for phylogeny, to test their predictive value in subtropical tree species.
Few surveys of C concentration in subtropical tree species have been conducted to date. Zheng et al. examined differences in C allocation in forests under different management practices14 and Zhuo et al. developed compatible C content models of individual trees in a Chinese fir plantation32. The extent of variation in C concentration among subtropical tree species has not yet been addressed in subtropical forest. Thus, we examined species- and tissues-specific variations in C concentrations in eight common tree species among two functional types: two gymnosperm species (Cunninghamia lanceolata and Pinus massoniana) and six angiosperm species (Alniphyllum fortunei, Choerospondias axillaris, Liquidambar formosana, Cyclobalanopsis glauca, Litsea rotundifolia and Schima superba). The objectives of this study were to: (1) examine variations in C concentrations among tree species; (2) determine whether functional type classifications, based on phylogeny and functional traits, correspond with variations in C concentrations among tree species; and (3) compare estimates of stand C stocks using species- and tissues-specific, functional type and generic C concentrations.
Materials and Methods
Site description
The study was conducted at the Paiyashan Forest State Farm (latitude 26°24′–26°35′N, longitude 109°27′–109°38′E) in Jingzhou County, Hunan Province, China. The altitude of the farm ranges from 330 m to 1075 m above mean sea level. The farm is in the humid mid-subtropical monsoon climatic zone. The average annual temperature is 16.7 °C and the mean annual precipitation is 1250 mm. The parent material is purple sand shale. At altitudes below 600 m, the soil type is red soil, and at altitudes above 600 m, the soil type is characterised as yellow soil. These soils are classified as Alliti-Udic Ferrosols in the Chinese Soil Taxonomy, which corresponds to Acrisol in the World Reference Base for Soil Resources33, 34. The farm encompasses C. lanceolata plantations and several secondary forests (e.g. coniferous and deciduous mixed, deciduous and evergreen broadleaved mixed, and evergreen broadleaved forests) dominated by many native tree species35.
Tree sampling and tissue sample preparation
According to leaf morphological and phenological traits36 and species composition, we sited eight forest types in Paiyashan Forest Farm. A 30 m × 30 m plot was established for each forest type. We selected 8 tree species in the eight forests which divided into two functional types, i.e., two gymnosperm species (C. lanceolata and P. massoniana) and six angiosperm species (A. fortunei, C. axillaris, L. formosana, C. glauca, L. rotundifolia and S. superba). Ten trees from each species were selected for C concentration sampling (except for 18 trees for C. lanceolata). Trees were selected to range in diameter at breast height (DBH) between the minimum and maximum observed values for a given species and along an even distribution. A total of 88 sample trees with DBH up to 0.51 m and height up to 30.2 m were harvested for C concentration measurements. Stand characteristics of the eight secondary forests, and tree species selected for C concentration measurement, are presented in Table S1.
All living branches were cut from canopy heights (upper, middle and lower) as close to the stem as possible and were immediately weighed. Three representative branches from each canopy height were selected, and all leaves were separated from every branch. Three fresh branch samples, weighing approximately 500–1000 g, were randomly sampled from three larger branches together with 3 corresponding leaf samples. A 5-cm thick disc was taken at 0–1.3 m, 1.3 m to half of total height and over half of total height of stem. Three wood discs were sampled from bark and stem without bark from each tree. A quarter sector of each wood disc was sampled for measuring C concentration and the remaining three-quarters was sampled for RGR and WD measurements. Roots were divided into coarse (diameter >2 mm) and fine roots (diameter <2 mm)37 and three samples were randomly collected from each. All samples were taken in October 2014 before leaf fall and transported to the laboratory for chemical analysis.
Measuring C concentration
All samples were oven-dried to a constant mass at 70 °C. The samples were ground to a fine powder using a ball mill (0.25 mm), and 20–30 mg of powder for each sample was used to determine C concentration. The C concentration was measured by the oil-bath K2Cr2O7–H2SO4 titration method14, 38, and expressed as % dry mass.
Measuring functional traits
We focused on two functional traits of leaves and three of stems because these traits are directly relevant to C sequestration, growth, survival and reproduction of trees39,40,41,42. LA (cm2) has important consequences for leaf energy and water balance22 and SLA (cm2 g−1) is an indicator of a trade-off in leaves between C gain and leaf longevity22. For stems, MAI (kg year−1) is the increase in biomass at the start of a given time interval. RGR (g kg−1 year−1) is a prominent indicator of plant strategy concerning productivity with environmental stress and disturbance regimes27. WD (g cm−3) captures trade-off in stems between growth and strength23. The data of these functional traits are easily obtained and are potentially correlated with C concentrations8, 22,23,24, 43.
To estimate LA and SLA, 15 intact and full grown leaves were randomly sampled at each canopy position (upper, middle and lower) from every tree. Leaves were then pooled to determine LA and weight for SLA. Each leaf length and width was measured to calculate LA. All leaves were washed with distilled water and oven-dried to a constant mass at 60 °C to measure the dry biomass for SLA.
A stem disk at breast height (1.3 m) from each tree sample was sanded and a digital scan was made with a scanner. Annual increments of DBH in the last 5 years were calculated on four directions (N, S, E and W) and averaged for each sampled tree. The values of MAI and RGR were calculated from annual incremental growth in tree biomass using allometric equations developed by Xiang35, as follows8, 44:
where M 1 and M 2 represent tree biomass at times t 1 and t 2, respectively.
WD was calculated as dry mass divided by field-moist wood volume for each sampled tree. A disk about 3–5 cm thick was sawed from the trunk at ~1.3 m height. The field-moist wood volume was measured by water-displacement. Wood samples were oven-dried at 100–110 °C to constant weight to obtain the dry mass.
Phylogenetic analyses of functional traits
Traits that evolve slowly are considered subject to phylogenetic “constraint” and, thus, have a phylogenetic signal. The phylogenetic signal in C concentrations of stem, bark, branch, leaf, coarse root and fine root as well as functional traits (i.e. WD, LA, SLA, MAI and RGR), was quantified using the K statistic45 performed in the “picante” package46 in R47. The K statistic compares a trait distribution from a phylogenetic tree to a distribution expected under a Brownian motion model of evolution that represents a continuous evolutionary change and random distribution across the phylogenetic tree. The K value was calculated by the following formula:
where the MSE0 is the mean squared error of the tip data calculated by the phylogenetically correct mean (MSE0) and the MSE is the mean squared error of the data measured by the variance-covariance matrix derived from the candidate tree45. A K = 1 implies that the observed trait distribution matches the Brownian motion model, while K < 1 implies more randomly distribution than a Brownian motion model and K > 1 implies higher phylogenetic signal or more conservatism than a Brownian motion model (i.e. trait similarity of related taxa)15, 45, 48. Statistical significance was tested by random permutation of traits across the tips of the phylogeny (n = 999). Traits were deemed significantly conserved if the observed K was in the upper 2.5% of the randomised K distributions. It should be noted that this null model of randomised K distributions corresponds to no phylogenetic signal, with Knull << 148. Phylogenetic trees were created with Phylomatic (v3) based on the Angiosperm Phylogeny Group (APG) III system49.
Estimates of forest stand C stock. We used the measurements from four forests in Dashanchong Forest Park50 (28°23′–28°24′N, 113°17′–13°19′E), Changsha County, Hunan Province, China, to quantify the error in stand C stock estimated using the generic C concentration constant (50.0%) and the C concentrations measured in this study. A 1-ha permanent plot was established for each forest and, within each, 20 m × 30 m subplots were established. There were seven subplots for C. lanceolata plantation (CLF), 15 for coniferous mixed forest (PMF), 16 for deciduous mixed forest (CAF) and 14 for evergreen broadleaved forest (CGF)51, 52.
The inventory of tree species, according to percentage of biomass, for all four forests was: (1) 97% C. lanceolata biomass and 3% other deciduous and evergreen angiosperm biomass in CLF; (2) 49% P. massoniana biomass, 7% C. glauca biomass and 44% other deciduous and evergreen angiosperm biomass in PMF; (3) 58% C. axillaris biomass, 2% L. rotundifolia biomass and 40% other deciduous angiosperm biomass in CAF; and (4) 14% C. glauca biomass, 13% C. axillaris biomass and 73% other evergreen broadleaved biomass in CGF. C stocks (t C ha−1) were estimated using the generic C concentration, C concentrations of tree species and tissues, and C concentrations of average value of all tree species in specific functional type measured, given as CSg, CSm and CSf, respectively.
Data analysis
Bartlett and Levene tests were performed to determine if the data satisfied homogeneity of variance. Analysis of variance (ANOVA) and Tukey’s honest significant difference (HSD) test were used to detect significant differences between tree species and tissues. One-way ANOVA was used to compare differences of stand C stock estimates between CSg, CSm and CSf. The relationships between C concentration and functional traits (LA, SLA, MAI, RGR and WD) were analysed using stepwise regression. Functional traits were selected as having significant effects on C concentrations according to the lowest Akaike information criterion (AIC). Phylogenetically independent contrasts (PIC) were tested using the “ape” package53 to determine the effect of phylogeny on the relationship between C concentrations and functional traits. All statistical analyses were performed in R47.
Results
Species- and tissue-specific variations in C concentration
Significant effects of species, tissues and their interaction on C concentrations were observed (Table S2). Tree species, tissues and their interactive effect accounted for 35.41%, 17.89% and 18.97% of the total variance in C concentrations, respectively. C concentrations in other tissues did not significantly differ among the canopy or stem positions (Fig. 1), except among branches (F 2,236 = 4.154, P = 0.017).
C concentrations differed significantly among tree species (P < 0.0001). Average C concentration was highest in C. lanceolata (47.9 ± 2.5%, mean ± SD) and lowest in A. fortunei (42.3 ± 1.0%) (Table 1). Overall, the order of tree species based on highest to lowest C concentrations was as follows: C. lanceolata > P. massoniana > L. rotundifolia > C. axillaris > S. superba > L. formosana > C. glauca > A. fortunei. C concentrations for a specific tissue also varied among tree species. The highest C concentrations were found for stems in P. massoniana, branches in L. rotundifolia and bark, leaves, coarse roots and fine roots in C. lanceolata among the eight tree species, whereas the lowest C concentrations were found for stems and coarse roots in C. glauca, bark in A. fortunei, branches in S. superba, leaves in L. formosana and fine roots in L. rotundifolia (Table 1).
For a given tree species, C concentrations significantly differed among tissues (P < 0.0001). From highest to lowest, the C concentration of tissues for each tree species ranked accordingly: leaf > bark > coarse roots > stem > fine root > branch for C. lanceolata; stem > branch = leaf > bark > coarse root > fine root for P. massoniana; stem > branch > leaf > coarse root > bark > fine root for A. fortunei; stem > branch > coarse root > leaf > bark > fine root for L. formosana; branch > bark > stem > leaf > coarse root > fine root for C. axillaris; branch > leaf > bark > stem > coarse root > fine root for L. rotundifolia; branch = leaf > stem > fine root > bark > coarse root for C. glauca; and leaf > stem > bark > branch > coarse root > fine root for S. superba (Table 1).
Phylogenetic distribution of C concentration and functional traits
The phylogenetic tree of tree species clustered into two distinct clades: one clade for the two gymnosperm species and another for the six angiosperm species, supporting the classification of trees into two broad functional types. K statistics for the C concentrations of stem (K = 1.57), branch (K = 1.54), coarse root (K = 1.92), LA (K = 1.41) and MAI (K = 1.17) indicated the phylogenetic conservation of these traits (Fig. 2). K statistics for the C concentrations of bark (K = 0.97), leaf (K = 0.72), fine root (K = 0.63), WD (K = 0.49), SLA (K = 0.88) and RGR (K = 0.56) indicated a random distribution (Fig. 2).
C concentrations and functional types
C concentrations in gymnosperm species (47.4 ± 2.6%) were significantly higher than those in angiosperm species (43.8 ± 2.4%) across all tissues (P < 0.0001; Fig. 3). C concentrations in gymnosperm species were greatest in leaf (48.8 ± 2.2%), while the highest concentrations occurred in stem (44.9 ± 1.6%) for angiosperm species. Fine root contained the lowest C concentration in both gymnosperm (45.4 ± 3.6%) and angiosperm (40.0 ± 3.3%) species.
C concentrations and functional traits
Significant negative relationships were observed between LA and average C concentrations of stems (F 1,6 = 6.089, P = 0.049), bark (F 1,6 = 30.53, P = 0.001), branches (F 1,6 = 9.803, P = 0.020), leaves (F 1,6 = 14.860, P = 0.008) and coarse roots (F 1,6 = 15.19, P = 0.008) across all eight tree species (Fig. 4). For all sampling angiosperms trees, C concentrations were negatively correlated with LA in stems (F 1,48 = 4.638, P = 0.036), bark (F 1,48 = 21.200, P < 0.0001), branches (F 1,48 = 22.090, P < 0.0001), leaves (F 1,48 = 10.780, P = 0.002) and roots (F 1,48 = 3.027, P = 0.088) (Fig. S1). Significant or marginal significant negative correlations between C concentrations of each tissue (except branch) and LA were apparent when testing for the influence of PIC (Fig. 4). There was no significant relationship between LA and C concentrations in fine roots (F 1,6 = 2.145, P = 0.193). There were no significant relationships between the average C concentrations of the eight tree species and any other functional traits: SLA, MAI, RGR and WD (Table S3).
Error in estimation of forest C stock
Stand C stocks estimated using generic C concentration (CSg) and C concentration of species and tissue (CSm) and functional type (CSf) were 37.00, 35.09 and 35.15 t C ha−1 for the CLF plantation, respectively. The average estimated C stocks in PMF forest were 81.61, 74.59 and 70.54 t C ha−1 for CSg, CSm and CSf, respectively; and correspondingly 61.72, 54.80 and 54.23 t C ha−1 for CAF; and 73.77, 65.00 and 64.81 t C ha−1 for CGF. Failing to account for the species- and tissue-specific variations in C concentration introduced a relative error of -1.06% to 13.48% in estimates of biomass C stocks from CSg and CSf. However, there was no significant difference (P > 0.05) between any estimations of C stock for any given forest (Fig. 5). Across the four forests, there were marginally higher C stocks of CSg than that CSm (P = 0.097) and no significant differences of CSf against CSg (P = 0.132) and CSm (P = 0.877).
Discussion
Variation among tree species substantially impacted measurements of C concentration. Seventy-two percent of variation in C concentrations was explained by species-specific tissue types and average C concentration differed significantly between tree species, ranging from 42.3% for A. fortunei to 47.9% for C. lanceolata. This finding is consistent with previous studies13. This range is lower than that found for temperate (43.7–55.1%)8, boreal (48.4–51.0%)10 and subtropical tree species (48.0–57.7%)14 in China. It is possible that the relatively low C concentrations in this study may be due to differences in climatic conditions16, 17 or tree size54, 55. Temperatures in temperate/boreal areas drop below zero and tree species there are known to produce higher molecular weight, C-rich compounds, such as lignin, phenolic acids, suberin and tannins56 which leads to higher C concentrations. For tree species growing in similar climatic conditions, tree size is considered to be an important factor. For example, average C concentrations in this study were similar for C. lanceolata, about 10.3% lower for P. massoniana and 5.4% lower for C. glauca compared to the results of Zheng et al.14. DBH was similar for C. lanceolata in this study (15.5 cm) and for Zheng et al. (15.1 cm); whereas the DBH range in the present study was greater for P. massoniana (5.9–52 cm) and C. glauca (6.2–50.9 cm) than found by Zheng et al. (8.2–23.7 and 3.2–11.1 cm, respectively).
C concentration differed significantly among stems, bark, branches, leaves, coarse roots and fine roots among tree species. This is in agreement with previous studies showing significant differences in C concentration in stems, branches, leaves and roots of balsam fir43 and in stems, new branches, old branches, leaves, coarse roots and fine roots across 10 Chinese temperate tree species8. Variation in C concentration among tree tissues was mainly driven by chemical composition. Many studies have shown that stem wood contains two principal chemical compounds: lignin (13.7–35.0% of dry mass) and holocellulose (65.0–75.0% of dry mass)16. Branches have similar chemical composition as stem wood, with lignin concentrations of 17.1–27.1% and holocellulose of 26.7–73.0% on dry mass bases57, 58. Leaves contain 17.8–35.3% lignin and 36.0–45.0% holocellulose on dry mass bases57, 59, 60. The C concentrations we observed were in accordance with the known relative differences in concentration of these compounds61, 62, where C concentrations of stems (45.9%) and leaves (45.9%) was higher than in branches (45.1%). Bark has evolved to resist biotic and abiotic stress, leading to similarly high concentrations of C-rich compounds (e.g. extractives, lignin, phenolic acids, suberin and tannins)54, 63. These compounds constitute above 40% of dry mass, and C concentration of these compounds is in the range of 59–62% (except for suberin with about 73%)16, which can result in higher C concentration in bark. In this study, C concentration was lower in bark (45.0%) than in stems, leaves and branches, while previous studies reported that C concentration was higher in bark than any other tissues54, 55. This difference may be due to species growing in different climatic conditions. The C concentrations of coarse (44.0%) and fine (42.0%) roots were lower than all other measured tissues. The concentration of low-C compounds (e.g. starch)64 in coarse roots and other low-C compounds in fine roots (e.g. free phenols, bound phenols, lignin phenols and non-structural carbohydrates), compared to other tissues65, 66, may explain these differences.
C concentrations were significantly higher for gymnosperm species than angiosperm species (Fig. 3) in this study. Many studies have reported higher C concentrations in gymnosperm species (46.9–57.72%) compared to angiosperm species (43.7–55.1%)8, 14, 16, 43. The possible explanation for this is that gymnosperm species generally have higher lignification in tissues20, different lignin chemistry19 and/or lower concentrations of non-structural carbohydrates17, 21. Lignin content can be approximately 30% in gymnosperm stems versus 20% for angiosperms9. In addition, Chinese subtropical forests consist of many tree species35, making it difficult to measure C concentration for each tree species. Variations in C concentrations among functional types (i.e. gymnosperms and angiosperms) could be a potential alternative to using species-specific C concentrations to estimate forest C stocks at local or large scales.
Estimation of C concentrations was also possible based on characterisation of functional traits. C concentrations of tissues were negatively correlated with LA. The negative relationships between C concentration and LA may be attributable to functional type given the significant phylogenetic signal for LA (Fig. 2). LA is morphological trait known to vary among ecological strategies of tree species27. Konôpka et al. (2016) noted that leaf size was related to light intensity26, 67. Under the same growth condition (e.g. CO2 concentration and light intensity), tree species with larger LA exhibit faster photosynthetic rate and higher growth rate than species with smaller LA24. In the line with Martin and Thomas15, the result of this study showed a negative relationship between C concentrations and RGR but the relationship was not significant. Actually, fast-growing tree species contain lower C-rich compounds (e.g. alkaloids, phenolic glycosides, cyanogenic glycosides), whereas slow-growing species contains higher C concentrations compounds (e.g. lignin, polyphenolic compounds)8. Accordingly, LA measurements could provide a reasonable basis for classifying functional types and for estimating the C concentration and C sequestration capacity of tree species.
Failing to account for differences in C concentrations among tree species and tissues introduced significant biases in forest C stock estimates, consistent with previous studies8, 16. Estimations of stand C stock using a generic C concentration was 1.91–8.76 t C ha−1 (5.45–13.48%) higher than that estimations using direct measurements of species- and tissue-specific C concentrations. Stocks estimation using functional type did not significantly differ from estimations using species- and tissue-specific C concentrations. In CLF, the CSm was just 0.06 t C ha−1 lower than CSf, as in CAF and CGF, the CSm was 0.57 and 0.19 t C ha−1 higher than CSf, respectively. In PMF, differences in estimated C stocks were as large as 4.05 t C ha−1. This magnitude of error may be due to the higher proportion of angiosperm species (51%) in this forest, which contain lower C concentrations than gymnosperm species. This observation emphasizes the value of basing C stock estimates on functional type when possible. Additional measurements of C concentrations from a greater variety of tree species and tissues will further improve our estimates and is necessary to validate the use of generalised functional types for estimating forest C stocks. Our study demonstrates that profiling forests based on tree functional type provides effective methods for assessing forest C stocks which will continue to improve in accuracy as data on C concentration expands.
Conclusions
The C concentrations differed significantly among tree species and tissues at our subtropical forest sites. Gymnosperm species exhibited significantly higher C concentrations than angiosperm species. We determined that the use of a generic conversion factor, without considering C concentration differences among tree species and tissues, overestimates C stocks in subtropical forests by 1.91–8.76 t C ha−1. Functional type classifications, by leaf morphology and phenological traits, provided comparable estimates of C stocks to those made with species- and tissue-specific C concentrations. Therefore, it is recommended to use functional types in assessing the C sequestration capacity of subtropical forests as it is straightforward and produces comparable results.
References
UNFCCC. UN-REDD Programme Strategic Framework 2016-20 (Revised draft - 7 May 2015), http://www.unredd.net/documents/policy-board-86/fourteenth-policy-board-meeting-washington-usa-20-22-may-2015/session-3-strategic-and-policy-issues/14096-un-redd-pb14-2015-strategic-framework.html (Date of access: 09/05/2017) (2015).
Yang, Y. H. et al. Increased topsoil carbon stock across China’s forests. Glob. Change. Biol. 20, 2687–2696 (2014).
Stocker, T. F. et al. IPCC, 2013: Technical Summary. In: Climate Change 2013: The Physical Science Basis (eds Stocker, T. F., D. Qin, G.-K. Plattner, M. Tignor, S. K. All.) (Cambridge University Press, 2013).
Kreidenweis, U. et al. Afforestation to mitigate climate change: impacts on food prices under consideration of albedo effects. Environ. Res. Lett. 11, 085001 (2016).
UNFCCC. Fact sheet: Reducing emissions from deforestation in developing countries: approaches to stimulate action http://unfccc.int/files/press/backgrounders/application/ pdf/fact_sheet_reducing_emissions_from_deforestation.pdf (Date of access: 09/05/2017) (2011).
Yeboah, D., Burton, A. J., Storer, A. J. & Opuni-Frimpong, E. Variation in wood density and carbon content of tropical plantation tree species from Ghana. New For. 45, 35–52 (2014).
Jayasekera, R. & Schleser, G. H. Seasonal changes in organic carbon content of leaves of deciduous trees. J. Plant Physiol. 138, 507–510 (1991).
Zhang, Q. Z., Wang, C. K., Wang, X. C. & Quan, X. K. Carbon concentration variability of 10 Chinese temperate tree species. For. Ecol. Manag. 258, 722–727 (2009).
Lamlom, S. H. & Savidge, R. A. A reassessment of carbon content in wood: variation within and between 41 North American species. Biomass Bioenerg. 25, 381–388 (2003).
Thomas, S. C. & Malczewski, G. Wood carbon content of tree species in eastern China: interspecific variability and the importance of the volatile fraction. J. Environ. Manag. 85, 659–662 (2007).
Fang, J., Guo, Z., Hu, H. & Son, Y. Forest biomass carbon sinks in East Asia, with special reference to the relative contributions of forest expansion and forest growth. Glob. Change Biol. 20, 2019–2030 (2014).
Li, P. et al. The relative contributions of forest growth and areal expansion to forest biomass carbon. Biogeosciences 13, 375–388 (2016).
Elias, M. & Potvin, C. Assessing inter- and intra-specific variation in truck carbon concentration for 32 neotropical tree species. Can. J. For. Res. 33, 1039–1045 (2003).
Zheng, H. et al. Variation of carbon storage by different reforestation types in the hilly red soil region of southern China. For. Ecol. Manag. 255, 1113–1121 (2008).
Martin, A. R. & Thomas, S. C. A reassessment of carbon content in tropical trees. PLoS One 6, e23533 (2011).
Bert, D. & Danjon, F. Carbon concentration variations in the roots, stem and crown of mature Pinus pinaster (Ait.). For. Ecol. Manag. 222, 279–295 (2006).
Thomas, S. C. & Martin, A. R. Carbon content of tree tissues: a synthesis. Forests 3, 332–352 (2012).
Harmon, M. E., Fasth, B., Woodall, C. W. & Sexton, J. Carbon concentration of standing and downed woody detritus: effects of tree taxa, decay class, position, and type. For. Ecol. Manag 291, 259–267 (2013).
Campbell, M. M. & Sederoff, R. R. Variation in lignin content and composition- Mechanism of control and implications for the genetic improvement of plants. Plant Physiol. 110, 3–13 (1996).
Minami, E. & Saka, S. Comparison of the decomposition behaviors of hardwood and softwood in supercritical methanol. Japan Wood Res. Soc. 49, 73–78 (2003).
Hoch, G., Richter, A. & Korner, C. Non-structural carbon compounds in temperate forest trees. Plant Cell Environ. 26, 1067–1081 (2003).
Diaz, S. et al. The global spectrum of plant form and function. Nature 529, 167–171 (2016).
Kunstler, G. et al. Plant functional traits have globally consistent effects on competition. Nature 529, 204–207 (2016).
Chave, J. et al. Towards a worldwide wood economics spectrum. Ecol. Lett. 12, 351–366 (2009).
Wright, I. J. et al. The worldwide leaf economics spectrum. Nature 428, 821–829 (2004).
Castaño-Santamaría, J. & Bravo, F. Variation in carbon concentration and basic density along stems of sessile oak (Quercus petraea (Matt.) Liebl.) and Pyrenean oak (Quercus pyrenaica Willd.) in the cantabrian range (NW spain). Ann. For. Sci. 69, 663–672 (2012).
Pérez-Harguindeguy, N. et al. New handbook for standardised measurement of plant functional traits worldwide. Aust. J. Bot. 61, 167–234 (2013).
Swenson, N. G. & Enquist, B. J. The relationship between stem and branch wood specific gravity and the ability of each measure to predict leaf area. Am. J. Bot. 95, 516–519 (2008).
Ackerly, D. D. Functional strategies of chaparral shrubs in relation to seasonal water stress and disturbance. Ecol. Monogr. 74, 25–44 (2004).
Cavender-Bares, J., Kitajima, K. & Bazzaz, F. A. Multiple trait associations in relation to habitat differentiation among 17 Floridian oak species. Ecol. Monogr. 74, 635–62 (2004).
Wright, I. J., Falster, D. S., Pickup, M. & Westoby, M. Crossspecies patterns in the coordination between leaf and stem traits, and their implications for plant hydraulics. Physiol. Plantarum 127, 445–456 (2006).
Zhuo, L., Tao, H., Wei, H. & Chengzhen, W. Compatible models of carbon content of individual trees on a Cunninghamia lanceolata plantation in Fujian province, China. PLoS One 11, e0151527 (2016).
IUSS Working Group WRB. World reference base for soil resources 2006 (World soil resources reports no. 103, 2006).
Gou, M. M. et al. Allometric equations for applying plot inventory and remote sensing data to assess coarse root biomass energy in subtropical forests. BioEnerg. Res. 10, 536–546 (2017).
Xiang, W. H. et al. Species-specific and general allometric equations for estimating tree biomass components of subtropical forests in southern China. Eur. J. For. Res. 135, 963–979 (2016).
Iio, A., Hikosaka, K., Anten, N. P. R., Nakagawa, Y. & Ito, A. Global dependence of field-observed leaf area index in woody species on climate: a systematic review. Glob. Ecol. Biogeo 23, 274–285 (2014).
Xiang, W. H. et al. Fine root interactions in subtropical mixed forests in China depend on tree species composition. Plant Soil 395, 335–349 (2015).
Dong, M. Survey, observation and analysis of terrestrial biocommunities (in Chinese) (Standards Press of China, 1997).
Diaz, S. et al. The plant traits that drive ecosystems: Evidence from three continents. J. Veg. Sci. 15, 295–304 (2004).
Poorter, L. et al. Are functional traits good predictors of demographic rates? Evidence from five Neotropical forests. Ecology 89, 1908–1920 (2008).
Weiher, E. et al. Challenging theophrastus: a common core list of plant traits for functional ecology. J. Veg. Sci. 10, 609–620 (1999).
Garnier, E., Navas, M. & Grigulis, K. Plant functional diversity organism traits, community structure, and ecosystem properties (Oxford University Press, 2016).
Xing, Z. S. et al. Carbon and biomass partitioning in balsam fir (Abies balsamea). Tree Physiol. 25, 1207–1217 (2005).
Castro, J., Reich, P. B., Sánchezmiranda, A. & Guerrero, J. D. Evidence that the negative relationship between seed mass and relative growth rate is not physiological but linked to species identity: a within-family analysis of scots pine. Tree Physiol. 28, 1077–1082 (2008).
Blomberg, S. P., Garland, J. R. T. & Ives, A. R. Testing for phylogenetic signal in comparative data: behavioral traits are more labile. Evolution 57, 717–745 (2003).
Kembel, S. W. et al. Picante: R tools for integrating phylogenies and ecology. Bioinformatics 26, 1463 (2010).
Core Team, R. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/ (2016).
Kraft, N. J. B. & Ackerly, D. D. Functional trait and phylogenetic tests of community assembly across spatial scales in an Amazonian forest. Ecol. Monogr. 80, 401–422 (2010).
Webb, C. O., Ackerly, D. D. & Kembel, S. W. Phylocom: software for the analysis of phylogenetic community structure and trait evolution. Bioinformatics 24, 2098–2100 (2008).
Zhu, W. J. et al. Spatial and seasonal variations of leaf area index (LAI) in subtropical secondary forests related to floristic composition and stand characters. Biogeosciences 13, 3819–3831 (2016).
Ouyang, S. et al. Significant effects of biodiversity on forest biomass during the succession of subtropical forest in south China. For. Ecol. Manag. 372, 291–302 (2016).
Liu, C. et al. Standing fine root mass and production in four Chinese subtropical forests along a succession and species diversity gradient. Plant Soil 376, 445–459 (2014).
Popescu, A. A., Huber, K. T. & Paradis, E. Ape 3.0: new tools for distance based phylogenetics and evolutionary analysis in R. Bioinformatics 28, 1536–1537 (2012).
Gao, B. L., Taylor, A. R., Chen, H. Y. H. & Wang, J. Variation in total and volatile carbon concentration among the major tree species of the boreal forest. For. Ecol. Manag 375, 191–199 (2016).
Martin, A. R., Gezahegn, S. & Thomas, S. C. Variation in carbon and nitrogen concentration among major woody tissue types in temperate trees. Can. J. For. Res. 45, 744–757 (2015).
Wiemann, C. M. & Williamson, G. B. Geographic variation in wood specific gravity: effects of latitude, temperature, and precipitation. Wood Fiber Sci. 34, 96–107 (2002).
Ngangyo-Heya M et al. Calorific value and chemical composition of five semi-arid Mexican tree species. Forests 7, 58 (2016).
Camarena-Tello, J. C. et al. Chemical composition of biomass generated in the Guava tree pruning. Excli J. 14, 204–212 (2015).
Newman, S. D., Soulia, M. E., Aber, J. D., Dewey, B. & Ricca, A. Analyses of forest foliage. I. Laboratory procedures for proximate carbon fractionation and nitrogen determination. J. Near Infrared Spectrosc. 2, 5–14 (1994).
Bolster, K. L., Martin, M. E. & Aber, J. D. Determination of carbon fraction and nitrogen concentration in tree foliage by near infrared reflectance: a comparison of statistical methods. Can. J. For. Res. 26, 590–600 (1996).
Adler, E. Lignin chemistry-past, present and future. Wood Sci. Technol. 11, 169–218 (1977).
Sjoestroem E. Wood Chemistry (Academic Press, 1993).
Vazquez, G., Antorrena, G. & Parajo, J. C. Studies on the utilization of Pinus pinaster bark. Wood. Sci. Technol. 21, 65–74 (1987).
Gholz, H. L. & Cropper, J. W. P. Carbohydrate dynamics in mature Pinus elliottii var. elliottii trees. Can. J. For. Res. 21, 1742–1747 (1991).
Wang, J. J., Tharayil, N., Chow, A. T., Suseela, V. & Zeng, H. Phenolic profile within the fine-root branching orders of an evergreen species highlights a disconnect in root tissue quality predicted by elemental- and molecular-level carbon composition. New Phytol. 206, 1261–1273 (2015).
Boldingh, H., Smith, G. S. & Klages, K. Seasonal concentrations of non-structural carbohydrates of five actinidia species in fruit, leaf and fine root tissue. Ann. Bot. 85, 469–476 (2000).
Konôpka, B., Pajtík, J., Marušák, R., Bošel’a, M. & Lukac, M. Specific leaf area and leaf area index in developing stands of Fagus sylvatica L. and Picea abies Karst. For. Ecol. Manag. 364, 52–59 (2016).
Acknowledgements
This work was supported by the National Key Research and Development Programme of China (2016YFD0600202), the National Natural Science Foundation of China (31570447 and 31170426) and Huitong Forest Ecological Station funded by State Forestry Administration of China. We thank the following post-graduates for their assistance in field investigations and laboratory chemical analysis: Zhaodan Liu, Yan Li, Renhui Li, Weixian Zeng, Mengmeng Gou, Hui Wei, Xiaoxiao Shen, Fangyong Yu and Xinhao Huang. Thanks also go to the staff of the administration office of Paiyashan State Forest Farm, Jinzhou County, Hunan Province, for their local support.
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Idea and study design: W.X., X.F. and P.L.; data collection and analysis: H.W., W.X., X.F., P.L. and X.D.; manuscript writing: H.W., W.X., P.L. and S.O.
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Wu, H., Xiang, W., Fang, X. et al. Tree functional types simplify forest carbon stock estimates induced by carbon concentration variations among species in a subtropical area. Sci Rep 7, 4992 (2017). https://doi.org/10.1038/s41598-017-05306-z
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DOI: https://doi.org/10.1038/s41598-017-05306-z
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