Abstract
d-myo-inositol 1,4,5-trisphosphate (InsP3) is a fundamental second messenger in cellular Ca2+ mobilization. InsP3 3-kinase, a highly specific enzyme binding InsP3 in just one mode, phosphorylates InsP3 specifically at its secondary 3-hydroxyl group to generate a tetrakisphosphate. Using a chemical biology approach with both synthetised and established ligands, combining synthesis, crystallography, computational docking, HPLC and fluorescence polarization binding assays using fluorescently-tagged InsP3, we have surveyed the limits of InsP3 3-kinase ligand specificity and uncovered surprisingly unforeseen biosynthetic capacity. Structurally-modified ligands exploit active site plasticity generating a helix-tilt. These facilitated uncovering of unexpected substrates phosphorylated at a surrogate extended primary hydroxyl at the inositol pseudo 3-position, applicable even to carbohydrate-based substrates. Crystallization experiments designed to allow reactions to proceed in situ facilitated unequivocal characterization of the atypical tetrakisphosphate products. In summary, we define features of InsP3 3-kinase plasticity and substrate tolerance that may be more widely exploitable.
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Introduction
The full scope of inositol polyphosphates (InsPs) function impacts on many facets of cell signaling and metabolism including DNA editing, mRNA export, Ca2+ signaling, vesicle trafficking, apoptosis and anticancer potential1,2. An established paradigm of InsP function is represented by InsP3 (d-myo-inositol 1,4,5-trisphosphate), a well-known second messenger involved in Ca2+ mobilization. Its binding to InsP3 receptors (IP3R) at the membrane of the endoplasmic reticulum triggers Ca2+ release from internal stores. InsP3 is recognized by two enzymes that process this messenger, so terminating Ca2+ signals, InsP3 3-kinase (IP3K) and InsP3 5-phosphatase (INPP5A). In particular, IP3K is a central protein in mammalian InsP metabolism3,4. It phosphorylates InsP3 yielding d-myo-inositol 1,3,4,5-tetrakisphosphate (InsP4) and is implicated in Ca2+ signaling regulation5,6 and in immune cell development7,8. In particular, the A isoform of IP3K, which is located in brain and testis, is important for dendritic morphology9, synaptic plasticity and impairments of learning and memory10. More recently, it has been shown that IP3KA is expressed ectopically in a broad range of tumor types, playing an important role for tumor growth and metastasis11,12,13.
IP3K is organized in several domains, including an N-terminal domain responsible for protein localization, a Calmodulin (CAM) binding domain responsible for protein activation, and a C-terminal catalytic domain with the kinase catalytic function. The N-terminal domain of the A and B isoforms comprises an actin binding domain that mediates localization to F-actin14,15, which is important for regulation of actin dynamics15. Currently, for IP3KA, only structures of the catalytic and CAM binding domains are available16,17,18. The structure of the IP3K kinase domain (IP3K-KD) revealed that the inositol polyphosphate kinase (IPK) family conserves the protein kinase (PK) fold organization in two lobes, the N- and C-lobes, binding the ATP in between them in a similar way. However, they present a third lobe, the IP-lobe inserted into the C-lobe, which accounts for most of the interactions with the inositide substrate. Available structures to date provide a full structural characterization regarding substrate (InsP3) and product (InsP4) recognition, revealing why IP3K is such a specific enzyme for InsP3 and also for the position of phosphorylation, the secondary hydroxyl group at position 3 (3-OH). From the IPK family, IP3K shows the greatest substrate specificity and selectivity, whereas inositol polyphosphate multikinase (IPMK) displays a great promiscuity, phosphorylating various InsP3 and InsP4 isomers at different positions. The shape of the IPKs IP-lobe is related to this aspect, IPMK exhibiting the smallest IP-lobe with just one helix19 whereas IP3K presents four helices that generate a more constrained active site16. InsP kinases from other families also contrast with IP3K high specificity, as is the case of the ITPK1 family of enzymes; however, this family displays a different fold that could account for this20.
Both IP3K activities, catalytic and F-actin bundling, have been linked to the oncogenic potential of IP3KA11. In support, inhibition of catalytic IP3K activity reduces proliferation and adhesion of lung cancer cells, diminishing their metastatic potential21,22. Inhibition based on the ATP binding site has been explored using purine derivatives23. In addition, some of us have explored targeting the InsP3 binding site based upon InsP3 analogues24. The chemistry of both natural inositol phosphates and their analogues has been thoroughly reviewed25,26. Finally, structurally more distant compounds were identified by high throughput screening, with IC50 values in the nM range22. Other targets, directed at the IP3R and based upon the natural product adenophostins have also been addressed, a recent example being a synthetic inositol ribophostin27,28,29.
Despite all this, it is not wholly clear what departures from the structure of InsP3 might be compatible with binding and even catalytic activity. Here, a diverse range of ligands related to InsP3 have been interrogated for their interaction with IP3K and ability to act as IP3K substrates using chemical synthesis, protein crystallography, HPLC analysis, molecular docking, thermal shift experiments and fluorescence polarization of a synthetic fluorescently-tagged FITC-InsP3. We have surveyed a subset of synthetic InsP3 and InsP4 analogues that includes both chemical variations in several positions of the inositol ring with respect to the natural substrate, including the critical 3-position28,29,30,31,32,33,34,35,36 and even related compounds based upon a non-cyclitol carbohydrate core. Our results shed light on IP3K active site plasticity and tolerance to different ligands and, of particular interest, on the surprising atypical IP3K phosphorylation of unexpected substrates. This work also defines unexpected IP3K biosynthetic capacities yielding innovative phosphorylated InsP-derived compounds, as well as providing a structural basis to aid future design of molecules of potential biomedical value.
Results
Synthesis of inositol phosphate ligands
We have used several InsP3 and InsP4 analogues (Fig. 1 and Supplementary Fig. 1) to analyze their experimental binding to IP3KA kinase domain (IP3K-KD). Syntheses of a number of these have been previously reported, as listed in the Methods Section, but three new analogues are employed in this study, scyllo-inositol 1,2,3,5-tetrakisphosphate (1-p), dl−6-deoxy-6-hydroxymethyl-scyllo-inositol 1,2,4-trisphosphate (5-s) and its phosphorylated product dl−6-deoxy-6-phosphoryloxymethyl-scyllo-inositol 1,2,4-trisphosphate (5-p). Synthetic routes are presented above (Fig. 2) and vide infra. [Note that for compounds 5-s and 5-p, used as racemates, only the l-enantiomers are shown in Fig. 1. These are the ones with phosphate regiochemistry directly comparable to InsP3 and that are selected by the enzyme. In addition to the standard lowest locant numbering used previously and in Fig. 1, the analogues 5-s and 5-p could also be formally named as d-3-deoxy-3-hydroxymethyl-scyllo-inositol 1,4,5-trisphosphate and d-3-deoxy-3-phosphoryloxymethyl-scyllo-inositol 1,4,5-trisphosphate, respectively, to make the comparison to the d-enantiomers of InsP3 and InsP4 more readily appreciated].
The central square represents the natural IP3K substrate (InsP3) and product (InsP4). The remaining compounds are mimics of substrates (-s) or products (-p) used in our experiments with the exception of compounds 6a–p and 7a–p, which were found in IP3K complexes generated from 6a–s and 7a–s respectively. We have classified these compounds into three groups: InsP3 (or InsP4) isomers or analogues (1–4); InsP3 (or InsP4) analogues with a primary hydroxyl (CH2-OH) at pseudo 3-position or carbohydrate equivalent (5–7) and ribophostin or analogues (8–9). Small arrows mark substituents that differ from InsP3 colored as InsP3 1 (yellow), 2 (red) and 3 (green) positions. Inner-squares show pairs of substrate/product mimics. The symbol of a P in a circle represents a phosphate group, with the phosphates transferred by phosphorylation (at pseudo 3-position) shown in green. The following specifies the nomenclature for compounds and the isomers used in this work: 1-s (l-scyllo-inositol 1,2,4-trisphosphate), 1-p (scyllo-inositol 1,2,3,5-tetrakisphosphate), 2-s (d-myo-inositol 1,4,6-trisphosphate), 3-s (d-3-deoxy-myo-inositol 1,4,6-trisphosphate), 4-s (l-chiro-inositol 2,3,5-trisphosphate), 5-s (dl−6-deoxy-6-hydroxymethyl-scyllo-inositol 1,2,4-trisphosphate), 5-p (dl-6-deoxy-6-phosphoryloxymethyl-scyllo-inositol 1,2,4-trisphosphate), 6a–s (β-d-glucopyranosyl 1,3,4-trisphosphate), 6a–p (β-d-glucopyranosyl 1,3,4,6-tetrakisphosphate), 7a–s (β-d-glucopyranosylmethanol 3,4,1’-trisphosphate), 7a–p (β-d-glucopyranosylmethanol 3,4,6,1’-tetrakisphosphate), 6b–s (α-d-glucopyranosyl 1,3,4-trisphosphate), 7b–s (α-d-glucopyranosylmethanol 3,4,1’- trisphosphate), 8 (ribophostin) and 9 (d-chiro-inositol ribophostin).
Reagents and conditions: (a) NaBH4/MeOH/THF, 89%; (b) i. 1 M HCl/MeOH 1:10, reflux; ii. conc. aqueous NH3, 68%; (c) i. (CEO)2PNPri2, 1H-tetrazole, CH2Cl2, ii. m-CPBA, –78 °C, 85%; (d) Na/liq NH3, –78 °C, 71%; (e) Me3NBH3, AlCl3, 4 Å sieves, THF, 0 °C, 23 h, 65%; (f) 1 M HCl/EtOH 1:2, reflux, 87%; (g) i. (BnO)2PNPri2, 1H-tetrazole, CH2Cl2, ii. m-CPBA, –78 °C, 85%; (h) Na/liq NH3, –78 °C, 71%; (i) 1 M HCl/EtOH 1:2, reflux, 71%; (j) i. (BnO)2PNPri2, 1H-tetrazole, CH2Cl2, ii. m-CPBA, –78 °C, 72%; (k) H2, Pd-C, MeOH, 40 p.s.i., 75%. Bn = benzyl, PMB = p-methoxybenzyl, CE = 2-cyanoethyl. All asymmetrical compounds are racemic.
Synthesis of scyllo-inositol 1,2,3,5-tetrakisphosphate (1-p)
Scyllo-Ins(1,2,3,5)P4 (1-p) possesses a plane of symmetry and therefore exists as a single meso-compound rather than as a pair of enantiomers. This makes it a particularly accessible analogue of Ins(1,3,4,5)P4 synthetically, in that neither a stereospecific synthesis nor an optical resolution is required. The synthesis (Fig. 2) began with the versatile 2-inosose (12)37, which was reduced rapidly and stereospecifically with sodium borohydride to the scyllo-inositol orthoformate derivative 13. Selective cleavage of the orthoformate ester gave tetrol 14. Phosphitylation with bis(2-cyanoethoxy)diisopropylaminophosphine in the presence of 1H-tetrazole, followed by oxidation of the intermediate tetrakisphosphite triester with 3-chloroperoxybenzoic acid (m-CPBA) gave fully-protected 15. Finally, removal of all protecting groups using sodium in liquid ammonia provided 1-p, which was purified by ion-exchange chromatography on Q Sepharose Fast Flow resin and isolated as the triethylammonium salt in 37% overall yield from 12.
Synthesis of dl-6-deoxy-6-hydroxymethyl-scyllo-inositol 1,2,4-trisphosphate (racemic 5-s)
Racemic 5-s was synthesized in four steps (Fig. 2) from the key racemic intermediate 1637 (Supplementary Methods and Supplementary Fig. 2). Thus, regioselective reduction of the benzylidene acetal in 16 using borane-trimethylamine complex and aluminium chloride in the presence of 4 Å molecular sieves gave the alcohol 17 in 65% yield. The two p-methoxybenzyl protecting groups of 17 were removed by acid hydrolysis giving triol 18, which was phosphitylated using bis(benzyloxy)diisopropylaminophosphine/1H-tetrazole. The 31P NMR spectrum of the intermediate trisphosphite triester showed a large 5JPP coupling of 6.1 Hz between the phosphorus atoms of the vicinal phosphite groups (values of 3 or 4 Hz are typical for vicinal phosphites in an inositol ring). Oxidation with m-CPBA gave the fully protected trisphosphate triester 19 that was a low-melting point crystalline solid. Deprotection using sodium in liquid ammonia, and purification by ion exchange chromatography as for 1-p went smoothly and the racemic 5-s was obtained as the pure triethylammonium salt.
Synthesis of dl-6-deoxy-6-phosphoryloxymethyl-scyllo-inositol 1,2,4-trisphosphate (racemic 5-p)
Racemic 5-p was also synthesized from intermediate 16 (Fig. 2) that was re-synthesized for this purpose essentially according to Riley et al.37. The benzylidene acetal and the two p-methoxybenzyl groups in 16 were removed together in one pot by treatment with refluxing ethanol/1 m aqueous HCl (1:2), giving tetrol 20 in 71% yield after chromatography. Phosphitylation of 20 with bis(benzyloxy)diisopropylaminophosphine/1H-tetrazole in dichloromethane gave a P(III) tetrakisphosphite that was not isolated, but the reaction mixture was checked by 31P NMR spectroscopy and signals observed at δ140.8 (doublet), 142.3 (singlet), 142.5 (doublet), and 143.0 (singlet). Immediate oxidation with excess m-CPBA at −78 °C, then purification of P(V) product by column chromatography gave the desired benzyl-protected tetrakisphosphate 21 in 72% yield. Deprotection was achieved by catalytic hydrogenolysis using palladium on charcoal in MeOH/water (4:1) to yield the desired tetrakisphosphate that was purified by ion-exchange chromatography on Q Sepharose Fast Flow resin, eluting with a gradient of triethylammonium bicarbonate buffer to yield racemic 5-p as the triethylammonium salt in 75% yield.
IP3K recognizes a variety of InsP3 mimics
We have obtained multiple crystals of IP3K-KD in the presence of most substrate (-s) and product (-p) mimics, as shown in Fig. 1. Compound numbering is shown in Supplementary Fig. 1. However, in this work we will also refer to their pseudo 1, 2, 3, 4, 5 or 6 positions, which are spatially equivalent to the 1, 2, 3, 4, 5 or 6 positions of InsP3 (Fig. 1) in their binding mode relative to natural InsP3. InsP3/InsP4 mimics can be classified into three groups regarding their chemical structure: (a) InsP3 (or InsP4) isomers or analogues (1–4); (b) 3-substituted InsP3 (or InsP4) analogues with a primary hydroxyl group (-CH2-OH) at the myo-inositol pseudo 3-position (5–7) and (c) ribophostin or analogues (8–9). Statistics for crystal data processing and data refinement are shown in Supplementary Table 1.
We observe overall that ligand binding is very conserved with that of InsP3 (Figs. 3, 4). Summarizing, IP3K substrate recognition entails close interactions with the three phosphates in positions 1, 4 and 5 of the InsP or of the corresponding carbohydrate-based analogues. Regarding the hydroxyls, the key 3-OH is recognized by Lys264 through hydrogen bonds, whereas no direct interactions are produced with OH groups in positions 2 and 6, though they keep van der Waals interactions with Met316 and Met288 respectively. In some of the complexes we observed the participation of two basic residues, Lys291 and Lys347, in a second sphere of InsP3 recognition. Interestingly, changing the natural InsP3 ring substituents leads to IP-lobe adjustments resulting in an helix tilt up to 9° in α2I generated by the contraction of the hinges connecting this helix to the protein (Fig. 5a, Supplementary Video 1). From now on we will refer to this motion as a spring effect. This effect on the IP-lobe, together with side chain adjustments, provides a plastic active site able to accommodate different ligands (Supplementary Fig. 3a–c).
A zoom of IP3K-ligand structures showing the ligand recognition site for InsP3 and InsP4 isomers (1–4). At the top of the figure the complexes with substrate InsP3 and product InsP4 properly numbered are shown to facilitate comparison (PDB codes 1w2c and 1w2d respectively). Protein is represented as cartoons showing important residues as sticks (N-lobe in blue, C-lobe in green and IP-lobe in gold), ligands as sticks (carbons in lemon for nucleotide and brown for inositide, oxygens in red, nitrogen in blue and phosphates in orange) and ions and waters as purple and red spheres respectively. Arrows link IP3K-substrate complexes with their related IP3K-product complexes.
A zoom of IP3K-ligand structures showing the ligand recognition site in 3-substituted analogues (5–7). Color code is the same as Fig. 3. Arrows link IP3K-substrate complexes with their related IP3K-product complexes. The IP3K complex with the expected product from the atypical substrate 5-s has been formed by crystal soaking with 5-p. IP3K crystal complexes with products 6a–p and 7a–p have been obtained by crystal soaking with the substrates 6a–s and 7a–s.
a Structural superposition of the twelve IP3K-ligand complexes obtained in this work including IP3K/InsP3 (code 1w2c) and IP3K/InsP4 (code 1w2d) complexes. IP3K structures are shown as cartoons highlighting the three lobes in different colours (colour code as detailed in Fig. 3). The main residues for ligand recognition are numbered and shown as sticks. InsPs and analogues are shown as brown thin sticks. On the right, two pictures of the IP-lobe from the IP3K complexes with 6a–p (red) and 5-p (pink colored), showing the maximum shift of the IP-lobe helix α2I. This shift is the spring effect referred to in the text, which consists of an helix-tilt of 9°. b Schematic representation of ligand positions superposed to the InsP3 position. InsP3 site is shown by InsP3 and 4-s harboring an axial OH in pseudo 2-position (left). By contrast, three types of deviations from the InsP3 site are found in the studied analogues represented by 1-s (compounds in this group: 1-s, 2-s, 3-s, 6a–s), 5-s (compounds in this group: 5-s, 6b–s) and 7a–s (right)). The small pictures show a superposition one by one displaying the shift direction by the arrows. Each ligand is colored according to the abbreviation at the top of its panel.
The above spring effect is coupled with a noticeable shift of the ligands away from the InsP3-site (Fig. 5b). We observed that this depends on the nature of the substituent at pseudo 2-position of the inositol ring. Thus, an axial OH in this position, (InsP3 and compound 4-s), forces the ligand to fit in the InsP3-site (Fig. 5b). However, all other InsP3 mimics used, with either an equatorial OH or no substituent at this pseudo 2-position, show a noticeable shift from the InsP3-site in different directions, depending on the analogue (Fig. 5b). Probably, either the van de Waals interactions produced between the axial 2-OH and Met316 or conserved water-mediated interactions between the equatorial 2-OH and three protein residues (Lys264, Arg331 and Glu333) could account for variations in the compounds’ binding relative to InsP3 (Figs. 3, 4).
After the catalytic reaction, the hydroxyl at position d−3 of the d-myo-inositol ring is phosphorylated and the protein maintains the ability to bind the product, at least at our experimental concentrations. Some of the compounds analyzed, such as 1-p, 5-p, 6a–p and 7a–p, are in fact mimics of the natural product InsP4 (Figs. 1, 3, 4). The complexes with 1-p and 5-p were obtained by soaking crystals directly with these product analogues. In contrast, complexes with 6a–p and 7a–p were obtained when crystals were soaked into solutions containing the substrate mimics 6a–s and 7a–s under specific conditions (see below). Substrate/product pairs only suffer a slight readjustment of the inositol polyphosphate position in the IP3K active site (Supplementary Fig. 3d). Otherwise, binding of product mimics obeys the same rules as before.
Finally, as with InsP3 itself, nearly all the complexes with substrate mimics keep the Mn2+ ion (Mn1) that bridges the ATP phosphates with the enzyme and orients Pγ for catalysis. Furthermore, we found up to three metal positions depending on the complex. Interestingly, complexes of IP3K with products formed in situ in the crystallization experiment exhibit two (6a–p) and three (7a–p) metal positions (Supplementary Fig. 4). The role of metal positions seems to be key in ATP binding, Pγ orientation, and transition state and product stabilization.
Improving IP3K ligands
Most ligands shown in Fig. 1 were submitted to ligand and thermal shift assays (Fig. 6). Noting that 2-FITC-InsP3 (Fig. 6a) proved a powerful ligand of the InsP3 binding site of an InsP3 receptor38, we tested this compound for binding to IP3K-KD under conditions with inclusion of EDTA, which prevents catalytic processing of the compound (Supplementary Methods and Supplementary Fig. 5). Curves for displacement of 2-FITC-InsP3 by diverse substrates, analogues and their products are shown, with derived IC50 values, in Fig. 6a and summarized in Supplementary Table 2. Thermal shift (denaturation) curves for IP3K-KD in the absence or presence of ligands are shown in Fig. 6b. Derived values of temperature of unfolding and its variation upon ligand binding are shown in Fig. 6b and Fig. 6c respectively and in Supplementary Table 3. Note that measurements from chemical and thermal shift experiments show high correlation (values of IC50 from Fig. 6a, as well as inferred Kd values, are shown as a bar at the bottom of Fig. 6c). We found that most compounds stabilize the enzyme significantly with the exception of ribophostin (8) and its analogue (9) (Fig. 6c). Note that compounds derived from the phosphorylated α-glucose anomer (6b–s) and the extended α-C-glycoside (7b–s) as InsP3 mimics stabilize IP3K-KD much less than InsP3 (Fig. 6c), probably due to the loss of interaction with Tyr315 (Fig. 4). Notably, it has not been possible to get crystal complexes with them, with the exception of compound 6b–s, that surprisingly was captured in the crystal.
a Displacement of 2-FITC-InsP3 from IP3K-KD by substrate and analogues, showing the structure of 2-FITC-InsP3. b Examples of thermal denaturation curves for apo IP3K-KD (without ligands) and IP3K-KD in the presence of selected InsP analogues. The graph on the right shows the first derivative of the curves. The colors for each ligand curve are indicated in the middle square. The dots on both graphs represent the Ti values of each experiment. The table below shows the mean of inflection temperature (Ti) values for the first transition of each curve, which show larger variations than the second transition. c Bar representation of mean ΔTi, showing the three individual points, in the first transition experimented by IP3K-KD upon each ligand binding. IC50 (and Kd) values are shown at the bottom to facilitate comparison. Error bars in (a) show the mean and 95% confidence interval from n = 4 discrete measurements of anisotropy of a single sample at the given protein: displacing ligand ratio. All samples were measured in a 384 well plate besides InsP3, which in two separate experiments (performed on separate occasions) gave IC50 values of 140.1 and 139.3 nM (data in Supplementary Table 2). The mean of Ti measurements from n = 3 and the respective SDs are represented in table (b) and the graph (c, error bars) (data in Supplementary Table 3). Source data are provided as a Source Data file.
All InsP3 (or InsP4) isomers (compounds 1–4) retain the ability to stabilize the enzyme significantly. We observe that the scyllo analogues of InsP3 and InsP4 (1-s and 1-p) with an inverted pseudo 2-OH group (therefore with all substituents equatorial) are better ligands for IP3K than the natural ones, exhibiting IC50 values of 36 nM and 51 nM respectively, compared to a value of 140 nM for InsP3. After these, the best compounds are those with just one substituent axial, either at pseudo 2 or 3 positions (InsP3 and compound 2-s). Finally, the presence of two axial -OH groups (4-s) constitutes the least stabilizing isomers in this group. Note that compound 3-s, with one axial -OH and the absence of a substituent at pseudo 2-position, present an equivalent effect to the latter one.
Analysis of the 3-substituted InsP3 analogues (compounds 5–7) revealed that a change in the pseudo 3-position substituent from a secondary and equatorial alcohol (-CHOH) as in natural InsP3, to a primary, but still equatorial, hydroxyl group (-CHCH2-OH), leads to a slight decrease in protein stabilization, although the analog still retains the ability to stabilize the enzyme significantly (Fig. 6c). The effect on stabilization is similar in the compounds derived from the scyllo-inositol template (5-s) and β-d-glucose template (6a–s). However, IC50 measurements indicate that the β-d-glucose derivatives (IC50 205 nM) are better ligands than the scyllo derivatives (IC50 972 nM), suggesting that a pyranoside oxygen placed at the pseudo 2-position is a promising feature (Fig. 1). Nevertheless, note that the scyllo derivatives (5-s and 5-p) are synthetic racemates and, due to the fact that IP3K only selects the l-isomer (vide supra for discussion of numbering), the IC50 measurements of this compound could likely be underestimated by a factor of two. The effects of the mirror image d-isomer, not selected in both crystals, are unknown but we presume they possess little or no binding activity.
Compounds of these series of 3-substituted analogues harbor different substituents at the pseudo 1-position. Analysis revealed that analogues derived from the α-glucose polyphosphate anomer (6b–s, IC50 8977 nM) and the corresponding C-glycoside (7b–s, IC50 6986 nM), i.e. with an axial pseudo 1-phosphate (1-P) instead of an equatorial one, barely stabilize the enzyme (Fig. 6). However, extending the phosphate position by changing the natural P substituent to a C-glycoside CH2-P group, but keeping the equatorial configuration (see 7a–s, IC50 656 nM, vs 6a–s, IC50 205 nM), results in a worse ligand by IC50 measurements, though still retaining a good ability to stabilize the enzyme.
Finally, product InsP4 and scyllo-analogues (1-p, IC50 51 nM, and 5-p, IC50 875 nM) behave similarly to their respective substrate and analogues, explaining why IP3K can easily be crystallized in the presence of products, at least at saturating concentrations.
In summary, changing the pseudo 2-OH configuration from axial to equatorial increases binding capability, whereas changing the configuration of other substrate substituents from equatorial to axial slightly decreases it. If the change of an equatorial group to an axial one involves a phosphate group, however, this decrease is dramatic. In a similar way, enlarging the -OH group and the phosphate groups at pseudo 3 and 1-positions by CH2-OH and CH2-P groups respectively, slightly decreases the binding capability, though to a significantly greater extent in the latter.
IP3K atypical phosphorylation on primary hydroxyls
The scyllo-InsP3 analogues (1-s and 1-p) as well as pseudo 3-substituted analogues (5-s, 5-p, 6a–s, 6b–s, 7a–s and 7b–s) were examined as IP3K substrates. HPLC analysis of reaction mixtures was performed to assess progress of the enzymatic reaction (Fig. 7a). We found that l-scyllo-1,2,4-trisphosphate (1-s) is a good IP3K substrate. In further support, the crystal structures in complex with substrate 1-s and its putative product, the synthetic symmetrical scyllo-inositol 1,2,3,5-tetrakisphosphate (1-p, in this study, equivalent to natural InsP4 in myo-inositol polyphosphate regiochemistry), show that they both perfectly mimic InsP3 and InsP4 binding (Fig. 3). The main difference is the shift from the InsP3 site in the scyllo-derived compounds (Fig. 5b). This shift causes a distance increase between the Pγ of ATP and the pseudo 3-OH of the inositol substrate by more than 1 Å (from 4.6 Å in the InsP3 complex to 5.8 Å in that for 1-s) (Fig. 7b). This confirms that a shift from the InsP3-site, and therefore a slight increase in the distance between reactive positions, is still compatible with catalysis.
a HPLC resolution of reaction products of assay of IP3K-KD with InsP analogues. With IP3K (black line), without IP3K (red line). ATP elutes at 8 min. InsP analogues and products obtained in the reaction thereof elute after 10 min. InsP analogues tested as substrates are indicated S, products of the reaction obtained are indicated P. b A zoom of the IP3K active site in complex with its natural substrate (left) and unexpected substrates (four pictures in the right); showing the distance between the reactive atoms (Pγ and O on 3-position - myo-inositol numbering) highlighted in orange (P) and red (O). Source data are provided as a Source Data file.
Unexpectedly, the InsP3 analogues substituted at the pseudo 3-position with primary hydroxyls are also substrates, as shown by HPLC analysis on reaction mixtures using compounds 5-s, 6a–s, and 7a–s, including their pseudo 1-axial analogues 6b–s and 7b–s, though the latter seem to be weaker substrates than the former (Fig. 7a). Since these compounds expand the putative reactive position by the one carbon-carbon covalent bond, this surprising finding was further characterized by X-ray crystallography.
Thus, we obtained crystal complexes of IP3K-KD with the atypical substrates 5-s, 6a–s, 6b–s and 7a–s (Fig. 4). In all cases, the primary hydroxyl occupies a similar position to the 3-OH from InsP3 remaining close to the ATP Pγ (Figs. 4, 7b). It should be noted that the synthetic 5-p used as a HPLC standard and 5-s compounds (Figs. 4, 7a) were racemic in this study, but only one enantiomer of 5-s, that mimicking the phosphate regiochemistry of d-InsP3, is assumed to interact with the enzyme to give the unique product 5-p. In concordance, the crystal structure of the IP3K-KD/5-p complex shows that 5-p binds mimicking the InsP4 phosphate regiochemistry (Fig. 4). Furthermore, HPLC with authentic, albeit racemic, synthetic standard shows that compound 5-p and the product of the reaction of compound 5-s coelute (Fig. 7a). We also explored different soaking conditions for IP3K-KD crystals in presence of ATP/Mn2+ plus 6a–s or 7a–s compounds shown to be substrates by HPLC (Fig. 7a). Increasing the ratio nucleotide/inositide enough to still capture the latter allowed the reaction to proceed and the crystals to capture the products of the reaction 6a–p and 7a–p (Fig. 4). These data reveal that phosphorylation unambiguously also occurs at the primary 6-OH hydroxyl group (carbohydrate numbering) i.e. also at a group placed at the pseudo 3-position (myo-inositol numbering) of the two atypical and chiral carbohydrate-based substrate mimics of InsP3.
These results represent a demonstration that IP3K, or an IPK, can not only phosphorylate more diverse compounds than those based on cyclitols. i.e. those based upon a carbohydrate template, but also at their primary hydroxyl group when they possess the appropriate regiochemistry of polyphosphate substitution. These data open up singular potential to harness the biosynthetic ability of this enzyme.
Docking of 3-substituted myo-InsP analogues and substrate ability
To identify further IP3K ligands and substrates virtually, we have validated for our system two docking strategies using the GOLD program, as described in Methods. Briefly, a first approach docking each ligand against its experimental protein structure and a second one, named ensemble docking, using all experimental protein structures instead. Both strategies yield solutions that approximately reproduce the overall aspects from the experimental results obtained from the IP3K-KD complexes, but only in the case of complexes with substrate mimics. Binding of product and its analogues (Supplementary Fig. 6a) was not predicted. It’s noteworthy that the ensemble docking, which introduces protein flexibility employing a repertoire of obtained experimental structures, improves the docking results compared to using only the previously available structure of IP3KA-KD in complex with InsP3 (code 1w2c) (see Supplementary Fig. 6b, c).
The predictive results using InsP3 mimics prompted us to search for binding models for the three compounds that failed to form protein-ligand crystal complexes (7b–s, 8 and 9). Thus, we obtained a model for the α–substituted carbohydrate-based 7b–s, which demonstrated poor substrate activity for IP3K. We hypothesize that this compound might require a slightly constrained conformation to effectively bind to IP3K, as suggested by the analysis of bond torsions (Supplementary Fig. 7a). This observation could potentially rationalize its limited substrate activity, as depicted in Fig. 7a. In parallel, models obtained for the two ribophostin-based analogues (8 and 9) show high energy conformations as compared with CSD geometrical distributions, which is in keeping with our failure to obtain crystal complexes or observe protein stabilization (Supplementary Fig. 7a).
Next, we searched for binding models for another two InsP3 analogues using ensemble docking. The first one is a more distant InsP3 known synthetic isomer (10) which presents several axial substituents (Supplementary Fig. 7b). The docking prediction for compound 10 suggests a rotation from the InsP3 position in order to properly allocate the phosphates. While we maintained the ring torsions rigid during the docking run, we have considered the two possible energetically related chair conformations, however, we cannot rule out the possibility that other cyclohexane conformations such as twist-boat could be selected by the enzyme.
The second one is based on the 3-substituted ligands (myo-inositol numbering) studied that lack an axial pseudo 2-OH. As mentioned above, these ligands cause a shift in the IP3K active site with respect to InsP3 and therefore allow enough room for the pseudo 3-CH2-OH to be accommodated (Fig. 7b). Therefore, in order to predict the binding to IP3K of an atypical myo-inositol substrate harboring an axial 2-OH group, otherwise equivalent to 5-s, we designed a compound (11) (Supplementary Fig. 7b) and submitted it to docking analysis. From the best solutions, we propose that this ligand could also bind to IP3K and, as in the case of 5-s, keep an adequate space between the Pγ and the primary hydroxyl (Supplementary Fig. 7c) though it would be more constrained. Further experimental validation is required to confirm the precise binding site of this ligand and its ability to be an IP3K substrate. This compound, to the best of our knowledge, has yet to be chemically synthesized.
From our analysis we therefore suggest that a variety of InsP3 mimics possessing an extended OH group at the pseudo 3-position could be IP3K substrates, as long as they can exploit the discrete IP3K active site plasticity. The possible extent of the 3-OH branching or other possible variations remains to be determined.
Discussion
IP3K biology is crucial for Ca2+ signaling and actin dynamics; overexpression of the enzyme in non-natural tissues is associated with cancer processes and metastasis11.The work presented here outlines unexpected IP3K ligands and catalytic activity on atypical substrates.
Regarding unexpected IP3K ligands, we show here that InsP3 and InsP4 analogues with discrete variations in pseudo 1, 2 and 3-positions are good IP3K ligands. In summary, equatorial hydroxyls of the inositol ring increase ligand affinity, although pyranose-based derivatives are also good ligands for IP3K. This is of particular interest given that such ligands can be synthesized generally in chiral form and any required protecting group chemistry is usually much easier, whereas inositol derivatives often need tiresome separation of enantiomers. It should be noted, however, that the enzyme selects one enantiomer with the required regiochemistry of phosphate substitution from a racemate, at least in our limited case here. We also observed that small changes at the pseudo 2-position of the InsP do not notably affect ligand binding or protein stability in agreement with previous observations24, although affinity may be affected. However, moderately enlarging the substituent at the pseudo 1-position decreases ligand binding whereas enlarging the substituent at pseudo 3-position has a much lower effect. Finally, at saturating concentrations, the product InsP4 and its analogues are good ligands for IP3K suggesting that the IPK family might be inhibited by its reaction products, as in the case of lipid kinases39.
Binding different ligands to IP3K revealed a protein plasticity. Previous studies showed that the IP3K IP-lobe presents great flexibility, displaying a noticeable structural change upon InsP3 binding (Supplementary Fig. 8)16. We show here that IP3K also displays a moderate protein plasticity centered in the α2I helix of the IP-lobe (Fig. 5a) that enables IP3K to bind different InsP3 analogues. Protein flexibility and plasticity have also been observed in other members of the IPK family. Thus, mammalian InsP5 2-K undergoes a change from an open conformation to sequential closed conformations upon nucleotide and InsP5 binding respectively40. Regarding IPMK, acting on several InsP3 and InsP4 isomers, we believe that its unique IP-lobe helix (equivalent to IP3K α4I) might also show plasticity (Supplementary Fig. 8d), though structural analysis in the presence of different InsP mimics would confirm this. The related lipid kinase PI3K has also shown great plasticity in the ATP site which is a good target point for selective inhibitor development against the different PI3K isoforms41. Finally, this is also a repeated feature in protein kinases, where structural plasticity is of the utmost importance for kinase regulation and activity, with different functional states showing distinct conformations42. Targeting specific kinase conformations is a strategy to obtain more selective inhibitors. Undoubtedly, IP3K plasticity could be also exploited to design InsP ligands specifically, including potential bi-substrate ligands that represent a successful approach in the PK field to develop molecules without off-target effects derived from targeting the conserved ATP binding43.
An exciting achievement in the field would be discovery of molecules able to discriminate between all the InsP3 recognizing proteins that cover crucial events such as Ca2+ signaling, cell survival or cell growth. Despite the IP3K protein plasticity mentioned, ligands with voluminous substituents at the pseudo 1-position, such as the bicyclic disaccharide-based InsP3 mimic ribophostin (8) or its inositol analogue (9), or even the C-glycoside InsP3 mimic44,45 7b–s, do not, or barely, bind IP3K as we show experimentally herein (Fig. 6b,c) and through computational studies (Supplementary Fig. 7a). In agreement, other ribophostin-related analogues have previously been found not to be IP3K ligands24. This is particularly interesting due to the fact they act as potent agonists of IP3R28. Conveniently, IP3R and IP3K do not share a similar ligand recognition site, IP3Rs displaying a more open site for phosphate P1 recognition than IP3K, as observed in their crystal structures16,46. We can conclude here that ligands with large substituents at the pseudo 1-position can be designed to bind to IP3R highly potently without affecting IP3K, which is something of particular interest. It may be harder though to achieve the reverse specificity. Nevertheless, from this study we can also expand previous observations regarding the impact of substituents on pseudo 2 or 3-positions in both InsP3 binding proteins. Thus, compound 5-s, synthesized and shown to be a good IP3K ligand in this work, is equipotent with InsP3 itself in releasing 45Ca2+ from permeabilized rabbit platelets47. Note that the structure of 5-s differs from InsP3 in the 2-hydroxyl (equatorial vs axial) and the 3-substituent (hydroxymethyl vs hydroxyl). Since the 2-hydroxyl group is not important for Ca2+ release26 or IP3K binding (this work and Poinas et al.24), we conclude that discrete enlargement of the substituent in pseudo 3-position does not yield selective ligands between both enzymes. However, increasing the substituent at the pseudo 3-position to a methylenephosphate group as in 5-p, the enzymatic product of 5-s and also synthesized in this work, abolishes the ability to stimulate cellular Ca2+ release48, as determined in permeabilized rat hepatocytes30. The activity of 5-s and inactivity of 5-p was expected, as 5-p bears the same relationship to the natural InsP3 metabolite InsP4 which does not release calcium. Therefore, if 5-p is considered to be an InsP3 surrogate, its inactivity indicates that the steric bulk of a phosphate attached to the primary hydroxyl group at the 3-position is not tolerated by the receptor. In contrast, we have observed that 5-p maintains quite good binding to IP3K (Fig. 6), altogether suggesting that exploring the substituent in (pseudo) 3-position can reverse the selection towards IP3K.
Regarding the unexpected IP3K catalytic activity, previous studies with InsP mimics revealed that an equatorial secondary alcohol OH group at the myo-inositol pseudo 3-position is necessary to allow IP3K catalysis24. Interestingly, we show here that IP3K ligands still retain the ability to be substrates when the length of this 3-substituent is increased by an additional -CH2- motif. This represents a highly unexpected finding, considering the high specificity of binding and position of phosphorylation of natural InsP3 substrate. To the best of our knowledge, IPKs, as well as other families of InsP kinases, phosphorylate biological substrates at secondary equatorial hydroxyl groups, with the exceptions of the InsP5 2-kinase and InsP6 kinase families, which phosphorylates axial hydroxyls and equatorial phosphate groups respectively49,50. Interestingly in cells, protein kinases mostly phosphorylate three different residues that act as nucleophiles: Ser (primary hydroxyl), Thr (secondary hydroxyl) and Tyr (tertiary aromatic hydroxyl). In small molecule kinases that also keep an in-line phosphoryl transfer mechanism (see below), we found examples of a variety of nucleophiles comprising not only primary and secondary alcohols, but also phosphate groups, guanidinium groups or acidic groups51. In this work we have proven that, like Ser/Thr PKs, IP3K and presumably other IPKs such as IPMK, are able to phosphorylate primary hydroxyls; however, we do not know if IPK phosphorylation could be expanded to other substrate types, as has been observed with other types of kinases. Perhaps some guided mutations could engineer proteins with surprising abilities.
From the structure, we can assume that the phosphoryl transfer reaction consists of an in-line attack by the nucleophilic -OH onto the Pγ of ATP16,51. How much the mechanism has associative or dissociative features is well discussed in the protein kinase field52,53,54. Some crystallographic studies support a dissociative mechanism, such as that with protein kinase A (PKA)55 and with protein phosphatase AtPFA-DSP154 that succeeded in identifying a metaphosphate-like intermediate close to a fully dissociative transition state. A theoretical analysis indicates that the reaction coordinate distance (i.e., the optimal distance between the entering oxygen and the phosphorus undergoing substitution) for a fully dissociative transition state should be ≥4.9 Å56. In this work, the identification of unusual substrates (InsP3 and compounds 1-s, 5-s, 6a–s and 7a–s) that expand the reaction center’s distance (Fig. 7b) from 4.6 Å (InsP3) to a range of 3.8–5.8 Å suggests that IP3K phosphorylates a range of substrates possibly with a strong contribution of a dissociative mechanism.
In conclusion, we have identified different limits for IP3K specificity, as well as defined unpredicted biosynthetic capabilities for this enzyme relevant to InsP3 signaling. Our structural study reveals a moderate IP3K plasticity that allowed us to identify potentially available areas in the IP3K active site that represent an opportunity to design unusual IP3K ligands and even unexpected IP3K substrates. We can define the following features for IP3K substrate tolerance, i.e. what an InsP3 analogue must exhibit to be an IP3K substrate: (1) it must contain three phosphates at the pseudo 1,4 and 5-positions in a six-membered ring, although this does not necessarily have to be a cyclitol, (2) its substituents must be accommodated inside active site plasticity margins, some of them established in this work and (3) it should feature an -OH group at a 3- or a pseudo 3-position, either as a primary or secondary hydroxyl, as confirmed by HPLC methodology and in situ capture of products by crystallography. Ultimately, by exploiting IP3K structural features, this work opens a window for generation of unprecedented and potentially valuable InsP derivatives that might be useful in both related cell biology and chemistry fields. Moreover, these could evolve into selective molecules for InsP binding enzymes (i.e. IP3K vs IP3R or other IPKs), which could be used for disease therapies or more accurately delineate unique roles for these pleiotropic biomolecules by in vivo cellular studies.
Methods
Synthesis of inositol phosphate analogues
2-FITC-InsP3 was synthesized as described38 and used as its triethylammonium salt. Thin layer chromatography (TLC) was performed on precoated plates (Merck TLC aluminum sheets silica 60 F254, Art no. 5554). The spots were visualized using phosphomolybdic acid, potassium permanganate, iodine or UV light. All evaporations were carried out under reduced pressure. Flash chromatography was carried out using silica gel (Merck Kieselgel 60, mesh size 0.040–0.063 mm). 1H and 13C NMR spectra were recorded on either JEOL GX270 or EX400 spectrometers. Chemical shifts are reported in ppm relative to standards (TMS for samples in CDCl3 and [2H]7-DMF; TMS or DMSO for samples in [2H]6-DMSO; external 85% aqueous phosphoric acid for 31P spectra; TSP or HDO for samples in D2O). 31P NMR spectra were recorded on JEOL FX-90Q or EX-400 spectrometers. Multiplicities are indicated as follows: s (singlet), d (doublet), t (triplet), dd (doublet of doublets), q (quartet), m (multiplet), td (triplet of doublets), tt (triplet of triplets) and br (broad signal). Routinely, NMR resonances were formally assigned by use of multi-dimensional techniques and for hydroxyl protons through D2O exchange. Melting points were determined using a Reichert-Jung Thermo Galen Kofler block and are uncorrected. Elemental analysis was carried out at the University of Bath microanalysis service. Mass spectra were obtained by fast atom bombardment (FAB) in a VG707E spectrometer. Ion-exchange chromatography was carried out on a LKB-Pharmacia medium pressure ion-exchange chromatograph on Q Sepharose Fast Flow resin eluting with a gradient of triethylammonium bicarbonate (TEAB) buffer. Fractions containing target compound were detected and quantified by a modification of the Briggs phosphate assays57. All final target compounds studied were used as their triethylammonium salts and were of >95% purity as judged by HPLC methodology as described58. Briefly, compounds were detected by post-column complexation with ferric ion after anion-exchange separation on a CarboPac PA200 (Dionex) column eluted with methane sulfonic acid59.
Scyllo-inositol 1,2,3,5-tetrakisphosphate (1-p)
2,4-Di-O-p-methoxybenzyl-1,3,5-O-methylidyne scyllo-inositol (13)
Ketone 1237 (1.98 g, 4.63 mmol) was dissolved in a mixture of THF (20 mL) and methanol (80 mL). Sodium borohydride (430 mg, 11.6 mmol) was added gradually and the mixture stirred at r.t. for a further 30 min. TLC showed complete conversion into a product with Rf 0.44 (ethyl acetate/hexane 1:1). Water (100 mL) was added and the product extracted with dichloromethane (3 × 100 mL). The combined organic phases were washed with brine and dried over MgSO4. Evaporation of solvents under reduced pressure gave a white solid, which was recrystallized from ethyl acetate/hexane to provide 13 (1.77 g, 4.12 mmol, 89%).
1,3-Di-O-p-methoxybenzyl-scyllo-inositol (14)
To a solution of 13 (1.0 g, 2.32 mmol) in methanol (50 mL) was added 1 M HCl (5 mL). The mixture was heated at reflux for 30 min after which TLC showed that most of the starting material (Rf 0.48, ethyl acetate/ hexane 1:1) had been consumed. The heating source was removed and concentrated ammonia solution (1 mL) was added. Stirring was continued for a further 30 min at r.t. and then the solvents were removed by evaporation under reduced pressure to give a solid residue which was extracted with hot ethyl acetate (5 × 50 mL). Evaporation of the combined extracts, followed by crystallization from methanol/ethyl acetate gave 14 (666 mg, 1.58 mmol, 68%).
4,6-Di-O-p-methoxybenzyl-scyllo-inositol 1,2,3,5-tetrakis[bis(2-cyanoethyl)phosphate] (15)
To a solution of bis(cyanoethoxy)diisopropylaminophosphine (516 mg, 1.90 mmol) in dry dichloromethane (2 mL) was added 1H-tetrazole (267 mg, 3.80 mmol). The mixture was stirred at room temperature for 10 min and then the tetrol 14 (100 mg, 0.238 mmol) was added. The mixture was stirred for a further 1 h, after which a 31P NMR spectrum showed a complex pattern of signals around 141 ppm. The mixture was cooled to –78 °C and m-CPBA (360 mg, 2.09 mmol) was added. The mixture was allowed to reach room temperature, and then diluted with ethyl acetate (50 mL). The solution was washed with 10% sodium sulphite solution, sat. NaHCO3 and brine (50 mL of each), dried (MgSO4) and evaporated in vacuo to give an oil. Purification by column chromatography (ethyl acetate/ethanol 5:1) gave the tetrakisphosphate triester 15 as a colorless oil (237 mg, 0.202 mmol, 85%).
Scyllo-inositol 1,2,3,5-tetrakisphosphate (1-p)
Using a three-necked flask, ammonia (~100 mL) was condensed at –78 °C, and a sodium excess was added for drying. The resulting deep blue solution was stirred for 30 min at –78 °C. Using a second three-necked flask kept at −78 °C a small volume (~30 mL) of the dry ammonia was then distilled over and sodium added, ensuring the solution remained blue-black for 10 min. Compound 15 (100 mg, 85.8 µmol), dissolved in dry dioxane (2 mL), was then added to the vigorously stirred mixture and after 5 min, the reaction quenched with methanol and then deionized water, whereupon solvents and ammonia were removed in vacuo. The residue, dissolved in deionized water (300 mL), was purified by ion-exchange chromatography on Q Sepharose Fast Flow resin, eluting with a triethylammonium bicarbonate buffer gradient (0 to 1 moldm−3) at pH 8.0. The target 1-p, as its glassy triethylammonium salt obtained after evaporation in vacuo, was eluted between 670 mM and 780 mM. Yield 61 µmol, 71%.
dl-6-Deoxy-6-hydroxymethyl-scyllo-inositol 1,2,4-trisphosphate (racemic 5-s)
dl-6-Deoxy-1,3-di-O-benzyl-6-benzyloxymethyl-2,4-di-O-p-methoxybenzyl-scyllo-inositol (17)
To an ice-cold mixture of compound 16 (300 mg, 0.427 mmol) and 4 Å molecular sieves (2 g) and THF (10 mL) under N2 were added borane-trimethylamine complex (190 mg, 2.60 mmol) and freshly pulverised aluminium chloride (345 mg, 2.59 mmol). The mixture was stirred at 0 °C for 23 h, after which time TLC (ethyl acetate/hexane 1:1) showed the reaction to be complete with conversion of starting material (Rf 0.57) into a product (Rf 0.49). Ether (50 mL) was added followed by ice-water (50 mL) and 1 M HCl (10 mL). The organic layer was removed and the aqueous layer re-extracted with a further 50 mL of ether. The combined organic extracts were washed with brine (100 mL), dried (MgSO4) and evaporated under reduced pressure to give an oily residue. Purification by flash chromatography (dichloromethane/ethyl acetate 20:1) gave the alcohol 17 as a colorless oil, which slowly solidified (195 mg, 0.271 mmol, 65%).
dl-6-Deoxy-1,3-di-O-benzyl-6-benzyloxymethyl-scyllo-inositol (18)
The alcohol 17 (200 mg, 0.284 mmol) was dissolved in ethanol (60 mL) and 1 M HCl (30 mL) added. The mixture was heated at reflux for 5 h and then the solvents removed by evaporation under reduced pressure. The residue was dissolved in dichloromethane (50 mL), washed with sat NaHCO3 and brine (50 mL of each) and evaporated to give an oily residue. Purification by flash chromatography (ethyl acetate/hexane 1:1) gave triol 18 as a white solid (115 mg, 0.248 mmol, 87%).
dl-6-Deoxy−3,5-di-O-benzyl-6-benzyloxymethyl-scyllo-inositol 1,2,4-tris(dibenzylphosphate) (19)
To a solution of bis(benzyloxy)diisopropylaminophosphine (356 mg, 1.03 mmol) in dry dichloromethane (3 mL) was added 1H-tetrazole (144 mg, 2.06 mmol). The mixture was stirred at room temperature for 20 min and then the triol 18 (80 mg, 0.172 mmol) was added and stirring was continued for 30 min. The mixture was cooled to –78 °C, m-CPBA (200 mg, 1.16 mmol) was added, and the cooling bath was removed. The mixture was allowed to reach rt and then diluted with ethyl acetate (50 mL). The clear solution was washed with 10% Na2SO3, 1 M HCl, sat. NaHCO3 and brine (50 mL of each) dried (MgSO4) and evaporated in vacuo, giving an oily residue. Purification by column chromatography (chloroform acetone 10:1) afforded 19 (183 mg, 0.147 mmol, 85%) as a colorless oil which slowly crystallized.
dl-6-Deoxy-6-hydroxymethyl-scyllo-inositol 1,2,4-trisphosphate (racemic 5-s)
The trisphosphate triester 19 (60 mg, 48 µmol) was deprotected as described for compound 1-p. Purification by ion-exchange chromatography on Q Sepharose Fast Flow Resin, as before gave the glassy triethylammonium salt of 5-s, which eluted between 450 mM and 550 mM TEAB. Yield 34 µmol, 71%.
dl-6-Deoxy-6-phosphoryloxymethyl-scyllo-inositol 1,2,4-trisphosphate (racemic 5-p)
dl-6-Deoxy-6-hydroxymethyl-1,3-di-O-benzyl-scyllo-inositol (20)
To 16 (400 mg, 0.57 mmol) in EtOH (20 mL) was added 1 M hydrochloric acid (10 mL). [NB for the full associated synthetic details to 16 from myo-inositol orthoformate for this route please see Supplementary Methods and Supplementary Fig. 2]. The mixture was heated to reflux for 3 h. Evaporation then chromatography (CHCl3/MeOH 9:1) gave the tetrol 20 (152 mg, 71%) as a white solid.
dl-6-deoxy-6-(dibenzyloxyphosphoryloxy)methyl-3,5-di-O-benzyl-scyllo-inositol 1,2,4-tris(dibenzylphosphate) (21)
To a solution of bis(benzyloxy)diisopropylaminophosphine (1.11 g, 3.21 mmol) in dry CH2Cl2 (3 mL) was added 1H-tetrazole (450 mg, 6.42 mmol). The mixture was stirred at room temperature for 30 min, then the tetrol 20 (150 mg, 0.401 mmol) was added. After 40 min the mixture was cooled to –78 °C and m-CPBA (57-86%, 1.1 g) was added. The mixture was allowed to reach room temperature and stirring was continued for 30 min, then CH2Cl2 (50 mL) was added. The solution was washed with 10% Na2SO3 solution (50 mL), saturated NaHCO3 solution (50 mL) and brine (50 mL) then dried with MgSO4. Evaporation followed by flash chromatography (CHCl3/acetone 5:1) gave 21 (410 mg, 72%) as a colorless oil.
dl-6-Deoxy-6-phosphoryloxymethyl-scyllo-inositol 1,2,4-trisphosphate (racemic 5-p)
To 21 (150 mg) in MeOH (40 mL) and water (10 mL) was added 10% palladium on activated charcoal (200 mg). The mixture was shaken under hydrogen at 40 psi at room temperature overnight. The mixture was then filtered through Celite and the solvents were evaporated. The residue was dissolved in water (100 mL) and purified by ion-exchange chromatography on Q Sepharose Fast Flow resin eluting with a gradient of triethylammonium hydrogen carbonate buffer (0–1 moldm-3). Evaporation gave the tetrakisphosphate 5-p (79 μmol, 75%) as a colorless glass.
The following other ligands investigated were synthesized as described, purified by ion exchange chromatography, quantified using a Briggs phosphate assay and used as their triethylammonium salts; they were >95% pure by NMR spectroscopy: l-scyllo-inositol 1,2,4-trisphosphate 1-s60, d-myo-inositol 1,4,6-trisphosphate 2-s34, d-3-deoxy-myo-inositol 1,4,6-trisphosphate 3-s36, l-chiro-inositol 2,3,5-trisphosphate 4-s35, β-d-glucopyranosyl 1,3,4-trisphosphate 6a-s59, α-d-glucopyranosyl 1,3,4-trisphosphate 6b–s59, β-d-glucopyranosylmethanol 3,4,1’-trisphosphate 7a–s, α-d-glucopyranosylmethanol 3,4,1’-trisphosphate 7b–s44,45, ribophostin 8 and d-chiro-inositol ribophostin 928.
Purification of IP3K samples
A construct encoding human IP3K-KD A isoform (residues 187–461) into pOPTG vector was used16. The protein was expressed in Escherichia coli BL21 star (DE3) strain. Cells were grown at 37 °C to OD600 = 1.0 then induced with 0.4 mM IPTG for 18 h at 16 °C. Protein purification was performed by adapting the reported protocol16. Cells were resuspended in buffer A (10 mM Tris pH 7.5 [4 °C], 200 mM NaCl and 2 mM DTT) and disrupted by sonication. Clarified cell lysate was diluted 1/4 in buffer B (10 mM Tris pH 7.5 [4 °C] and 2 mM DTT) and loaded into a 5 mL HiTrap heparin column (GE Healthcare) washed by buffer C (20 mM Tris pH 7.5, 50 mM NaCl and 2 mM DTT), and eluted with a 0.05 M–1 M salt gradient. The eluted sample was subsequently loaded on to a 5 mL glutathione–Sepharose HP column (GE Healthcare), washed with buffer A and eluted with reduced glutathione 10 mM (Sigma-Aldrich) in buffer A. The GST tag was cleaved with home-made TEV (tobacco etch virus) protease overnight at 4 °C, (1:40 TEV/protein mass ratio). After glutathione removal with a PD10 column, the cleaved protein was further purified using a 5 mL glutathione–Sepharose HP column (GE Healthcare) followed by gel filtration on a 16/600 Superdex200 (GE Healthcare) column equilibrated in buffer D (20 mM Tris pH 7.5 [4 °C], 0.05 M (NH4)2SO4 and 2 mM DTT). Protein samples were analyzed by SDS/PAGE, concentrated around 18 mg/mL and stored at −80 °C until use.
Crystallization of IP3K complexes and structural determination
We produced well-diffracting IP3KA-KD crystals starting from the reported crystallization conditions16. Optimal crystals were obtained at 18 °C using the vapor diffusion method in sitting drop from 48-well plates (Hampton) and as precipitant solution 0.80–0.84 M tri-sodium citrate, 0.1 M Tris-HCl pH 8.5 (r.t.) and 0.1 M NaCl. To prepare ligand-protein complexes, we gradually changed the mother liquor of the crystal drops to the soaking solution (1.8 M Li2SO4 and 100 mM Tris-HCl pH 8). The last addition of soaking solution contained the ligands: nucleotide (ATP, AMPPNP or ADP depending on the complex, all of them from Sigma), inositide (Fig. 1 and Supplementary Fig. 1) and divalent ion (from MgCl2 or MnCl2 depending on the complex). Crystals were left, from 2 h to o/n, in the soaking solution. The soaking solution was itself a cryoprotectant. For a standard experiment we used 3 mM nucleotide, 3 mM divalent ion and 5 mM InsP3 analogue. Standard experiments were successful with compounds 1–4 series, 6a–s, 6b–s and 7a–s (Fig. 1). When using ligands that were not well captured in previous conditions because of poor binding or their status as racemates, as in the case of compounds 5-s and 5-p, we used 3 mM nucleotide, 3 mM divalent ion and 10 mM inositide (5-s) or 30 mM inositide in the citrate condition using 30% glycerol as cryoprotectant (5-p). Finally, conditions to produce reactions in the crystallization experiments from atypical substrates (6a–s and 7a–s) to get the products (6a–p and 7a–p) in the crystals were: 10 mM ATP, 10 mM divalent ion and 7 mM inositide into the soaking solution. Complete datasets from crystals were collected at 100 K in ALBA and ESRF synchrotron facilities. Datasets were processed using XDS61 and scaled with Aimless from the CCP4 suite62 or with autoPROC63. The crystal complexes structures were solved using Synthesis Fourier Difference using the coordinates of IP3KA-KD from PDB database (PDB code 1w2c). Several rounds of refinement using Refmac564 were alternated with model building using COOT65. The final processing and refinement statistics for best datasets obtained are reported in Supplementary Table 1. The crystals show two molecules in the asymmetric unit, in all cases molecule A being much more ordered than molecule B. Therefore, all our conclusions are based on the analysis of molecule A of the complexes in which electron density maps allowed unambiguous fitting of all the ligands. When the ligand structure was not in databases, ligand coordinates and cif files were obtained using PyMOL, COOT and Mercury programs65,66,67 and Grade server (https://grade.globalphasing.org/cgi-bin/grade2_server.cgi). The images were prepared with PyMOL.
Fluorescence thermal shift analysis
Thermal protein denaturation was documented by recording intrinsic protein fluorescence at 330 and 350 nm wavelengths. The fluorescence recorded in each thermal run is plotted as 350/330 nm ratio and used to calculate the Inflection Temperature (Ti). Ti is the temperature at which a protein undergoes a transition in its folding state. This calculation is performed automatically by the Tycho NT.6 software. The protocol optimized for this procedure, involves a heating time of 2 min, a sample volume of 10 μl placed in a very thin capillary, and a temperature ramp from 35 °C (308 K) to 95 °C (368 K). For the experiment, IP3K-KD was diluted with buffer D to achieve a concentration of 0.31 mg/mL (10 μM). It was then mixed with 100 μM of chiral ligands or 200 μM of racemic ligands (all synthesized as stated in this work except InsP3 and InsP4 that were commercial from Avanti Polar Lipids), AMP-PNP (substrate analogues) or ADP (product-like ligands) and 100 μM MgCl2. Microsoft Excel was used to calculate and plot ΔTi values and standard deviations.
HPLC analysis
For HPLC experiments, 150 nM IP3K-KD was incubated with 500 μM inositol phosphate substrate or analogue and 1 mM ATP under ATP-regenerating conditions in 20 mM HEPES pH 7.5, 1 mM MgCl2 for 2 h at 30 °C, with subsequent HPLC analysis as described58.
Ligand displacement assays
Binding of 2-FITC-InsP3, a small molecule, to a much larger protein slows the ‘tumbling’ of the probe on timescale comparable to the lifetime of its fluorescence. This results in an increase in the polarization (or anisotropy) of fluorescence emission. Binding of a competing/displacing ligand to protein is observed as a decrease in the polarization (anisotropy) of 2-FITC-InsP3. A comparison of the strength of binding of enzyme substrates, analogues and products to IP3K-KD was obtained by measurement of fluorescence polarization (here anisotropy) of 2-FITC-InsP3/IP3K-KD complex. Experimentally, 100 nM IP3K-KD was mixed with 2 nM 2-FITC-InsP337 in 20 mM HEPES pH 7.5, 1 mM EDTA and 50 mM KCl with the addition of displacing compounds over a range of 1 nM–50 μM. Assays were analyzed using a ClarioSTAR plate reader and IC50 values determined from fit of the data to a 4-parameter logistic as described68. From IC50 values, we have estimated the Ki (Kd) values for the displacing ligands using the equation of Cheng and Prusoff:69
where [L] is the ligand (2-FITC-InsP3) concentration and Kd the dissociation constant for its interaction with IP3K-KD. Fitting of the binding of 2-FITC-InsP3 to IP3K-KD with a one-site binding model in GraphPad Prism v.6 (GraphPad Software Inc., San Diego, USA) yielded a Kd of 68 nM. Consequently, the Ki values for the displacing ligands differ trivially from their IC50 values.
Docking
Docking experiments and visualization were performed with GOLD and Hermes, programs respectively (CCDC Software Ltd70.). GOLD (Genetic Optimisation for Ligand Docking) uses a genetic algorithm to explore a wide range of ligand conformations able to interact with the target protein active site. GOLD uses a quality function of fit that allows a solution score considering the bonds generated in the docking70. We chose the default ChemPLP function because it has optimized the time consuming and success degree ratio for prediction of ligand poses. The ChemPLP function score is dimensionless and takes into account the number and geometry of hydrogen bonds, hydrophobic contact area, ligand conformation and interactions with metal or water molecules to evaluate ligand protein fitting70. To make a comparison of the different ligands, the ChemPLP function scores were normalized against the molecular weight of each ligand. For our analysis, we added hydrogen atoms to the ligands, defined all protein conformations as rigid bodies and ligands as flexible elements (except ring torsions that have been kept rigid), and kept the nucleotide and Mn2+/Mg2+ atoms in the active site as intrinsic constituents. First, we made an initial validation of GOLD in our system, checking the reproducibility of experimental complexes structures obtained in this work. We did this in two ways: (1) using each of the ligands with the protein conformation in their complexes. Regarding water molecules, we input those bridging protein and ligand, allowing rotation freedom and intermittence to keep them or not. The active site was dimensioned to 6 Å around the ligand position and we searched for 10 solutions in each ligand run. The rest of the parameters were used as program default. (2) An ensemble docking that tests all ligands against all protein conformations obtained in experimental structures. The active site was defined as all atoms in a 10 Å radius sphere centered on the inositol ring (centroid x = 12.17 Å, y = 28.52 Å, z = 84,28 Å). We selected eight water molecules with intermittence possibility, rotation freedom and translation to 1.5 Å. We searched for 50 solutions for each ligand, keeping the remaining parameters as before. We have repeated the procedure with the latter settings searching the ligand poses against the protein structure of the complex IP3K:InsP3 (1w2c) in order to compare with the ensemble docking. We checked that this method is suitable for predicting the main characteristics of IP3K-KD substrate analogues interactions (Supplementary Fig. 6, 7), but found it was unsuitable to predict product and product analogues interactions. Finally, we built other putative IP3K ligands coordinates and cif files using Mercury and Grade Server as described before. We performed docking experiments in a similar way to ensemble docking, considering in this case the 10 best solutions.
Data availability
The authors declare that the main data supporting the findings of this study are available within the article and its Supplementary Information files. The atomic coordinates and structure factors of all structures generated in this study have been deposited in the PDB database (Research Collaboratory for Structural Bioinformatics, Rutgers University, New Brunswick, NJ (www.rcsb.org/)) under accession codes 8PP8, 8PP9, 8PPA, 8PPB, 8PPC, 8PPD, 8PPE, 8PPF, 8PPG, 8PPH, 8PPI and 8PPJ. The atomic coordinates used in this study are available in the PDB database under accession codes 1w2c, 1w2d, 1w2f and 5w2i. Source data are provided with this paper.
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Acknowledgements
We would like to acknowledge Paula Sanz Benito for valuable technical assistance. We are grateful to the staff of the Synchrotron Radiation Sources at Alba (Barcelona, Spain) for providing access and for technical assistance at BL13-XALOC beamline. We also acknowledge the European Synchrotron Radiation Facility ESRF (Grenoble, France) and the staff of beamlines ID23-1 and ID23-2 for assistance and support. M.A.M.-M. and this work has been supported by grants PID2020-117400GB-100 and BFU2017-89913-P from the Spanish Ministerio de Ciencia e Innovación and Ministerio de Economía y Competitividad. B.V.L.P is a Wellcome Trust Senior Investigator (grant 101010). This research was supported by The Wellcome Trust. For the purpose of Open Access, the authors have applied a CC BY public copyright license to any Author Accepted Manuscript version arising from this submission.
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B.G., C.A.B. and B.V.L.P. designed and supervised the research. M.A.M.-M. performed the constructs preparation, protein purification, crystallization, data collection, structural determination, docking analysis and thermal shift experiments. R.O.-G. helped in the constructs and sample preparation, protein crystallization and data collection. L.I. supervised docking analysis. S.G., A.M.R. & M.L.S. synthesized the inositol and carbohydrate compounds with supervision from B.V.L.P. and H.W. performed the HPLC and fluorescence polarization assays with supervision of C.A.B. B.G. and M.A.M.-M. analyzed the crystallographic data. M.A.M.-M., B.V.L.P. and B.G. wrote the paper. M.A.M.-M., L.I., M.L.S., C.A.B., A.M.R., B.V.L.P. and B.G. edited the manuscript.
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Márquez-Moñino, M.Á., Ortega-García, R., Whitfield, H. et al. Substrate promiscuity of inositol 1,4,5-trisphosphate kinase driven by structurally-modified ligands and active site plasticity. Nat Commun 15, 1502 (2024). https://doi.org/10.1038/s41467-024-45917-5
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DOI: https://doi.org/10.1038/s41467-024-45917-5
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