Abstract
Organic molecules that contain alkyl-difluoromethyl moieties have received increased attention in medicinal chemistry, but their synthesis in a modular and late-stage fashion remains challenging. We report herein an efficient copper-catalyzed radical relay approach for the carbo-difluoromethylation of alkenes. This approach simultaneously introduces CF2H groups along with complex alkyl or aryl groups into alkenes with regioselectivity opposite to traditional CF2H radical addition. We demonstrate a broad substrate scope and a wide functional group compatibility. This scalable protocol is applied to the late-stage functionalization of complex molecules and the synthesis of CF2H analogues of bioactive molecules. Mechanistic studies and density functional theory calculations suggest a unique ligand effect on the reactivity of the Cu-CF2H species.
Similar content being viewed by others
Introduction
The rapid construction of fluorine-containing organic molecules has attracted considerable attention, owing to the special ability of a fluorine atom or a fluoroalkyl group to modulate the metabolic stability, lipophilicity, and membrane permeability of a drug candidate1,2,3,4,5. Among the many fluorine-containing groups, the difluoromethyl group (CF2H) is drawing ever-increasing attention due its unique ability to act as a possible lipophilic hydrogen bond donor and thus a potential bioisostere for hydroxyl, amino, or thiol groups6,7,8,9,10,11,12,13,14,15,16,17,18,19,20. It is not surprising, therefore, that pharmaceutical companies began to investigate CF2H-containing lead compounds and intense research has been focused on the development of efficient methods for the installation of CF2H groups. Over the past decade, significant progress has been made in the subject of difluoromethylation of arenes21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37. However, analogous methods for the synthesis of alkyl-difluoromethanes have lagged behind, despite the high pharmaceutical relevance of these molecules (Fig. 1a).
Vicinal carbo-difluoromethylation of widely available alkenes, which results in the simultaneous formation of an alkyl–CF2H bond and a C–C bond, is an attractive, efficient, and potentially modular method for the synthesis of complex alkyl-difluoromethanes. Elegant work has recently been conducted by Dolbier38, Qing39, Xiao40, Gouverneur41, Zhang42, Chu43, and others6,44 for the difluoromethylation of alkenes by harnessing the reactivity of an in situ generated difluoromethyl or difluoroalkyl radical. Although these state-of-the-art methods allow for the rapid difluoromethyl-functionalization of alkenes, nonetheless, in these reactions the CF2H group is generally attached to the terminal side of the alkenes, as dictated by the CF2H radical addition to the less sterically hindered site. In addition, the groups installed to the remaining site of the alkenes are limited to either hydrogen atoms or other simple functional groups (Fig. 1b). Therefore, an alternative strategy allowing for the introduction of a diverse range of groups onto alkenes with complementary regioselectivity would open an avenue for the discovery of CF2H-pharmaceuticals.
Our group has recently demonstrated that copper can catalyze the efficient transfer of a CF2H group to an alkyl radical. This mode of CF2H transfer allowed us to develop the first decarboxylative45, deaminative46 and benzylic C–H difluoromethylation reactions47. These reactions proceed via copper-catalyzed generation of an alkyl radical which can be trapped by a [CuII-CF2H] intermediate and go through reductive elimination to generate the desired alkyl-difluoromethane. We recently wondered whether this Cu/CF2H system could be applied to the difunctionalization of alkenes by intercepting the radical intermediate with a suitable alkene to form a relayed radical48,49,50,51,52,53,54. Subsequent copper-CF2H trapping/reductive elimination of the relayed radical could then allow for the carbo-difluoromethylation of the alkene. We anticipated that, by varying the radical precursors and alkene partners, this approach could enable the synthesis of complex alkyl-difluoromethanes in a modular fashion (Fig. 1c).
A plausible mechanism for the proposed carbo-difluoromethylation reaction is shown in Fig. 2. A transmetalation of a copper(I) catalyst 1 with a [ZnII-CF2H] reagent is expected afford a reactive [CuI-CF2H] species 323,26,28,33,34,55, which should readily reduce a suitable alkyl or aryl electrophile 4, to form a carbon-centered radical 5. This radical species would then undergo a radical addition to an alkene 6, to generate the resultant relayed radical 7. The subsequent radical recombination of 7 with the [CuII-CF2H] complex 8 could generate a formal CuIII intermediate 956, which undergoes reductive elimination to forge the desired alkyl-difluoromethane product 10. This reductive elimination step regenerates the CuI catalyst and closes the catalytic cycle57,58. We recognized that a critical factor to the success of this reaction would be the selective addition of the carbon-centered radical 5 to the alkenes in lieu of direct recombination to the [CuII-CF2H] species. We expected that controlling the rates of radical recombination to the copper catalyst would be necessary to achieve the desired transformation. It is noteworthy that although difunctionalization of alkenes via a copper-catalyzed radical relay process—the concept of which was first conveyed by Liu and Stahl59—has been reported60,61,62,63, the simultaneous formation of two Csp3–Csp3 bonds via this process remains rare64.
In this work, we report a copper-catalyzed carbo-difluoromethylation reaction that can simultaneously introduce CF2H groups and complex alkyl or aryl groups into alkenes with the regioselectivity opposite to traditional CF2H radical chemistry.
Results
Difluoromethyl-alkylation of alkenes
Based on our previous work on the decarboxylative difluoromethylation of redox-active esters (RAEs), we first questioned whether an alkyl radical generated in situ from RAEs could participate in this reaction, allowing for the development of the difluoromethyl-alkylation of alkenes. Thus, we began our studies with the RAE of tetrahydropyran-4-carboxylic acid 11 as the alkyl electrophile, with styrene as the alkene partner. Different copper salts, ligands, and CF2H sources were evaluated (Fig. 3). To our delight, we found that the use of CuCl as the catalyst, along with (terpy)Zn(CF2H)2 (terpy = 2,2ʹ:6ʹ,2ʺ-terpyridine) as the CF2H source, generated via mixing terpy and (DMPU)2Zn(CF2H)2 in situ, the product 12 could be formed in a 76% yield in dimethyl sulfoxide (DMSO) at room temperature (r.t.) (entry 1). The formation of 12 represents a rare example of the simultaneous formation of two Csp3–Csp3 bonds via a copper-catalyzed radical relay process. In addition, the side product 12a, derived from the direct trapping of the alkyl radical by the [CuII-CF2H] species, was formed in a 11% yield under the optimized conditions. Intriguingly, the ligand used in the reactions plays a vital role in this transformation. No desired products were formed when styrene was added to our previously established decarboxylative difluoromethylation conditions, in which 2,2ʹ-bipyridine (bpy) was used as the ligand (entry 2). Other bidentate ligands provided essentially no products (Supplementary Table 1) and lower yields were observed when other substituted terpy ligands were used instead of terpy (entry 3). No desired product was formed when (DMPU)2Zn(CF2H)2 was used as the difluoromethyl source without the addition of terpy (entry 4). Trace amounts of products were formed when the analogous N-hydroxyphthalimide (NHPI) ester was used (entry 5). Different copper(I) and copper(II) salts could also be used (entries 6 and 7 and Supplementary Table 2), albeit with lower efficiency. Varying the amounts of (terpy)Zn(CF2H)2 and increasing the reaction temperature led to diminished yields (entries 8–10). The styrene loading can be reduced to 2 equiv., yet attaining a synthetically useful yield (entry 11). No difluoromethylated products were formed when the reactions were conducted in the absence of copper catalysts (entry 12). This reaction could also be set up outside of the glovebox without affecting the yield (entry 13 and Supplementary Method 1).
With the optimized conditions in hand, we began to evaluate the scope of vinyl arenes (Fig. 4). A wide range of electron-withdrawing, electron-donating, and electron-neutral functional groups could be tolerated on the aryl rings (12–28). The reactivity was not hindered by ortho substitutions on the vinyl arene ring (16). A basic tertiary amine group, which is usually problematic in transition metal catalysis, was tolerated (18). The compatibility with an aryl chloride (22) provides the basis for the further synthetic manipulation of the difluoromethylated products. This protocol allowed for the efficient installation of nitrogen- and oxygen-containing heterocycles, including benzofuran (26), unprotected indole ring (27), and pyrazole (28), which are ubiquitous in pharmaceuticals, into alkyl-CF2H moieties in good yields. It is worth noting that the excess vinyl arenes could be recovered with excellent mass balance (Supplementary Method 1).
We next turned our attention to the scope of carboxylic acids for this protocol. To our delight, this reaction was amenable to a wide range of secondary and tertiary carboxylic acids. RAEs derived from secondary carboxylic acids appended to a variety of four-, five-, six-, or seven-membered rings, paired with different functional groups could be successfully employed to deliver the desired products in good to excellent yields (29–45). Oxygen-containing saturated heterocycles, such as tetrahydrofuran (36, 37) and tetrahydropyran (38), were successfully employed in this reaction. Carboxylic acids derived from piperidine, one of the most prevalent ring systems found in drug molecules65, could undergo efficient difluoromethylation (40–43). Nitrogen-containing heterocycles, which are core structures in drug synthesis, including pyrimidine (40, 42) and pyridine (41), were all accommodated in this protocol. Two bicyclic and spirocyclic carboxylic acids were successfully converted to the desired products with good efficiency (44, 45). Furthermore, acyclic and cyclic tertiary carboxylic acids could undergo the radical relay pathway to give the desired products in good yields (46–51). Moderate yields were observed when a phenyl group was connected to the tertiary carboxylic acid (49), likely due to the lower reactivity of a tertiary benzylic radical. In addition to RAEs, activated alkyl bromides (52, 53) and an alkyl pyridinium salt (37) were found to participate readily in this reaction, providing the corresponding alkyl-CF2H products in good yields. Poor yields were observed when the RAEs of primary carboxylic acids were employed in the reactions and the decarboxylative difluoromethylated products were found to be the dominant products.
To further showcase the synthetic utility of this carbo-difluoromethylation protocol for the construction of complex alkyl-difluoromethanes, we sought to apply it to the late-stage modification of pharmaceuticals and natural products. Modification of a lipid regulating agent, gemfibrozil, afforded the desired alkyl-difluoromethane 54 in a 62% yield. Furthermore, we undertook the rapid functionalization of two complex steroidal acids, glycyrrhetinic acid and oleanolic acid, both of which could be converted to the corresponding difluoromethylated products in synthetically useful yields (55–57). The fact that unprotected hydroxyl groups could be well tolerated in these transformations further highlighted the mild conditions of this copper-catalyzed process. Given the ubiquity of carboxylic acids and alkenes, we expect that this protocol will find wide application in the field of medicinal chemistry for the synthesis of complex CF2H-containing pharmaceuticals in a modular fashion.
Difluoromethyl-arylation of alkenes
Having demonstrated that in situ generated alkyl radicals can participate in this carbo-difluoromethylation protocol, we further questioned whether aryl radicals could also be engaged in this reaction, allowing for the simultaneous introduction of an aryl and a CF2H group to various alkenes. A possible source for aryl radicals could be aryl diazonium salts, which are readily prepared from various anilines. We reasoned that an aryl diazonium salt could undergo a homolytic bond cleavage in the presence of a reactive [CuI-CF2H] species, forming an aryl radical34, which could react with an alkene via the Meerwein-type arylation pathway. The relayed radical could be trapped by a [CuII-CF2H] species, forming the desired difluoromethylated product via the radical recombination/reductive elimination pathway. It is noteworthy that although the Meerwein arylation reaction, which was originally reported by Hans Meerwein in 193966, has been developed as a useful method for the functionalization of alkenes67, the interception of the relayed radical by a copper catalyst for the construction of a C–C bond remains elusive.
We have found that in the presence of [Cu(CH3CN)4]BF4 (20 mol%) as the catalyst, terpy (20 mol%) as the ligand and (DMPU)2Zn(CF2H)2 as the difluoromethyl source, various aryl diazonium salts could undergo efficient difluoromethylation with different alkenes as the radical acceptors (Fig. 5 and see Supplementary Table 3 for optimization details). Aryl diazonium salts bearing electron-withdrawing or electron-donating groups reacted efficiently with vinyl arenes, affording the corresponding difluoromethylated products in good yields. Functional groups including halides (61–63), esters (64), ketones (68), and acetals (68) were well tolerated in these transformations. Ortho substitutions of the aryl diazonium salts did not hinder the reactivity (63). Aryl diazonium salts bearing heterocycles, such as dioxane (67), coumarin (69), and thioazole (70, 71), along with a heteroaryl diazonium salt (72) afforded the desired difluoromethylated products albeit in moderate yields. In addition to styrenes, other alkenes including acrylonitrile and methyl acrylate, could also be engaged in this difluoromethyl-arylation reaction, allowing for the rapid synthesis α-CF2H nitriles and α-CF2H esters (73–82). In addition, the difluoromethyl-arylation of acrylonitrile could also be conducted on a large scale. Without any modification of the standard conditions, the gram-scale synthesis of 76 could be produced in a similar yield to that obtained on a 0.25 mmol scale. Given the diverse reactivities of cyanide groups and ester groups, we expected that α-CF2H nitriles and α-CF2H esters could serve as highly useful building blocks for the preparation of other CF2H-containing moieties. For example, the treatment of an α-CF2H nitrile 76 with an alkaline solution of H2O2 afforded an α-CF2H amide 83 in an 80% yield, while the reduction of 81 with diisobutylaluminium hydride (DIBAL-H) allowed for the synthesis of a β-CF2H alcohol 84 in an 85% yield.
The synthetic utility of this difluoromethyl-arylation reaction has been further highlighted by the rapid synthesis of the CF2H analogue of combretastatin, 85, in a 45% yield. Combretastatin and its derivatives are potent vascular targeting agents and are undergoing various clinical trials for treating different cancers68. Considering the known ability of a CF2H group to act as a bioisostere of a hydroxyl group, 85 could potentially be a metabolically stable analogue of combretastatin.
Mechanistic studies
Mechanistic studies were conducted to shed light on these carbo-difluoromethylation reactions (Fig. 6). In our previously reported decarboxylative difluoromethylation reactions, bpy was found to be the optimal ligand45, but the tridendate ligand, terpy, was uniquely effective for the current system. We were intrigued by the dramatic difference of the difluoromethylation reactivity with difference ligands. Mechanistic understanding of the effect of ligands on the difluoromethylation reactivity is vital for the development of other alkyl-CF2H bond-forming reactions and could shed light on copper-catalyzed C–C bond-forming reactions that involve alkyl radicals as intermediates.
When the carbo-difluoromethylation reaction of 86 was conducted with bpy as the ligand, trace amount of the desired product 87 was observed. Analysis of the reaction mixture by GC/MS showed that the dimerization of the relayed radical (88) was a major competing pathway (Fig. 6a and Supplementary Figs. 11–12). We hypothesized that the reason for this dramatic difference in reactivity with different ligand is that the relayed radical, a secondary benzylic radical, could smoothly react with the [(terpy)CuIICF2H] intermediate but could not be efficiently trapped by a [(bpy)CuIICF2H] species.
Several additional experimental results further support this hypothesis. When a RAE derived from a secondary benzylic carboxylic acid, 89, was subjected to the standard decarboxylative difluoromethylation conditions, trace amount of the difluoromethylated product 90 was detected with bpy as the ligand, a phenomenon we observed in our previous studies45. On the contrary, a nearly quantitative yield was observed for the formation of 90 when terpy was used as the ligand. Similarly, the RAE derived from an acyclic secondary carboxylic acid 91 could undergo efficient decarboxylative difluoromethylation with terpy as the ligand, forming 92 as the major product, while a poor reactivity was observed with a bpy ligand under otherwise identical conditions (Fig. 6b). More importantly, in both cases, the dimerization of the secondary alkyl radicals was the competing reaction when bpy was used as the ligand (Supplementary Figs. 5–10).
To take into account of an alternative possibility that this ligand effect is due to the differences in reactivity of the [CuI-CF2H] species to undergo a single electron transfer event, we conducted the decarboxylative difluoromethylation of a radical clock substrate 93 (Fig. 6c). Direct decarboxylative difluoromethylation of 93 with either terpy or bpy as ligands under otherwise identical conditions both afforded the ring-opened product 94 in high yields along with trace amounts of the unrearranged product 95. Although further experiments are necessary for the detailed comparison of the reactivity of different [CuI-CF2H] species, these results support that both [(terpy)CuI-CF2H] and [(bpy)CuI-CF2H] species were reactive enough to undergo the single electron transfer events with the RAE 93 to generate the corresponding benzylic radical, which rearranged to a primary alkyl radical, affording 94 as the major product. The high yields of 94 with either ligand could be explained by the small steric interaction when a primary radical reacts with the [CuII-CF2H] species.
DFT calculation on the effect of ligands
Our mechanistic studies suggest that acyclic secondary alkyl radicals could be more efficiently trapped by the [CuII-CF2H] species coordinated to a terpy ligand. To better understand this ligand effect, we performed DFT calculations to probe the reaction between a secondary benzylic radical and the [CuII-CF2H] species bound with a bpy or a terpy ligand (Fig. 7). Our previous DFT calculations69 and Cook’s work70 on the trifluoromethylation of benzylic C–H bonds have both indicated that the benzylic radical could recombine with a four-coordinate [(bpy)CuII(CF3)2] species to form a five-coordinate high-valent copper(III) intermediate, [(bpy)CuIII(CF3)2(benzyl)], which reductively eliminates to form the trifluoromethylated product. Very recently, the Shen group has successfully characterized the putative five-coordinate copper(III) complex, [(bpy)CuIII(CF3)2(Me)], which could undergo C–CF3 bond-forming reductive elimination to form 1,1,1-trifluoroethane58. These precedents suggest that Csp3-fluoroalkyl bond-forming reductive elimination would most likely occur on five-coordinate copper(III) complexes.
With this background in mind, we reasoned that when bpy was used as the ligand for the difluoromethylation reaction, a benzylic radical would react with a four-coordinate copper(II) intermediate which bound to two CF2H groups, [(bpy)CuII(CF2H)2] (Cu-2, Fig. 7), to form a five-coordinate copper(III) complex (Cu-2A), which reductively eliminated to form the difluoromethylated product. On the contrary, when the tridentate ligand, terpy, bound with the copper center, the four-coordinate copper(II) could only attach to one CF2H group (Cu-1). The recombination of the benzylic radical with Cu-1 could form the five-coordinate high-valent copper(III) complex (Cu-1A), with only one CF2H bonding to the copper center.
Interestingly, our DFT calculation found that the formation of the high-valent CuIII species (Cu-1A) from the combination of a benzylic radical (Rad) with Cu-1 was a barrierless pathway. In contrast, the reaction between Rad with Cu-2 required a high activation energy (ΔG‡ = 21.8 kcal/mol, TS2), suggesting that the combination of the secondary benzylic radical, Rad, with Cu-1 was a much more efficient process than its reaction with Cu-2. It is noteworthy that Cu-2 was found to combine with a cyclohexyl radical via a barrierless pathway (Supplementary Fig. 13), consistent with our previous experimental results that the Cu/bpy system was effective for the decarboxylative difluoromethylation of the RAEs derived from cyclic secondary carboxylic acids.
Moreover, our calculation results demonstrated that the C–CF2H bond-forming reductive elimination step also contributed to the effect of ligands. As shown in Fig. 7, the reductive elimination of Cu-1A proceeded with a significantly lower activation energy (ΔG‡ = 6.3 kcal/mol, TSre-1) than that of Cu-2A (ΔG‡ = 18.1 kcal/mol, TSre-2). Overall, these DFT calculations suggest that the success of the tridentate ligand, terpy, in the carbo-difluoromethylation reactions was largely due to a rapid combination of the secondary benzylic radicals with the CuII intermediates as well as the facile reductive elimination of the CuIII species, although other factors including the difference of the transmetalation rates could not be ruled out.
Discussion
In conclusion, we reported herein a carbo-difluoromethylation of alkenes via a copper-catalyzed radical relay strategy, complementing the well-established CF2H radical addition to alkenes. This transformation allows for the rapid installation of CF2H groups and a diverse range of alkyl and aryl groups onto various vinyl arenes and Michael acceptors. This protocol could enable the efficient construction of complex alkyl-difluoromethanes in a modular fashion and rapid evaluation of structure–activity relationships of potential CF2H-containig pharmaceuticals. Furthermore, the difluoromethyl-alkylation protocol represents a rare example of a copper-catalyzed radical relay process for the simultaneous construction of two Csp3–Csp3 bonds, while the difluoromethyl-arylation reaction could open an avenue for the development of other Meerwein-type arylation reactions. Mechanistic studies indicated a previously unknown effect of ligands on the reactivity of the putative [Cu-CF2H] species, which may serve as a guide in selecting ligands in other copper-catalyzed reactions which involve alkyl radicals as the intermediates. Further studies on the use of other electrophiles/radical acceptors and the asymmetric version of this reaction are currently ongoing in our laboratories.
Methods
Difluoromethyl-alkylation of alkenes
In a nitrogen-filled glovebox, to a 4 mL vial equipped with a stir bar was added terpyridine (0.8 equiv., 47 mg), (DMPU)2Zn(CF2H)2 (0.8 equiv., 87 mg), and 800 μL DMSO. The resulting mixture was stirred at room temperature for 1 min to generate (terpy)Zn(CF2H)2 in situ. A different 4 mL vial equipped with a stir bar was sequentially charged with CuCl (30 mol%, 7.5 mg), RAEs (0.25 mmol, 1.0 equiv.), the DMSO solution of the in situ formed (terpy)Zn(CF2H)2, and alkene (0.75 mmol, 3.0 equiv.) in DMSO (200 μL). The resultant mixture was stirred at room temperature for 12 h. After the reaction was completed, the mixture was diluted with ethyl acetate (50 mL), filtered through a short plug of Celite, and washed with H2O (50 mL) and brine. The organic layer was combined, dried over Na2SO4, filtered, and then concentrated under reduced pressure. The crude product was purified by flash column chromatography.
Difluoromethyl-arylations of alkenes
In a nitrogen-filled glovebox, a 4 mL vial equipped with a stir bar was charged with [Cu(MeCN)4]BF4 (20 mol%, 15.5 mg), terpyridine (L1) (20 mol%, 11.8 mg), alkenes (0.75 mmol, 3.0 equiv.), and 200 μL DMSO. A solution of diazonium salts (0.25 mmol, 1.0 equiv.) in 400 μL DMSO, and a solution of (DMPU)2Zn(CF2H)2 (0.2 mmol, 87 mg, 0.8 equiv.) in 400 μL DMSO were slowly added to the vial with syringes at the same time over the course of 10 min. The resultant mixture was stirred at room temperature for 30 min. After the reaction was completed, the mixture was diluted with ethyl acetate (50 mL), filtered through a short plug of Celite, and washed with H2O (50 mL) and brine. The organic layer was combined, dried over Na2SO4, filtered, and then concentrated under reduced pressure. The crude product was purified by flash column chromatography.
Data availability
The authors declare that all data supporting the findings of this study are available within the article and its supplementary information file, and also are available from the corresponding author upon reasonable request. The experimental procedures and characterizations of all new compounds are provided in Supplementary Information. The X-ray crystallographic coordinates for structures reported in this study have been deposited at the Cambridge Crystallographic Data Centre (CCDC), under deposition numbers CDCC 2033193 (27), and CDCC 2033194 (43). These data can be obtained free of charge from www.ccdc.cam.ac.uk/data_request/cif.
References
Purser, S., Moore, P. R., Swallow, S. & Gouverneur, V. Fluorine in medicinal chemistry. Chem. Soc. Rev. 37, 320–330 (2008).
Muller, K., Faeh, C. & Diederich, F. Fluorine in pharmaceuticals: looking beyond intuition. Science 317, 1881–1886 (2007).
Harper, D. B. & Ohagan, D. The fluorinated natural-products. Nat. Prod. Rep. 11, 123–133 (1994).
Wang, J. et al. Fluorine in pharmaceutical industry: fluorine-containing drugs introduced to the market in the last decade (2001–2011). Chem. Rev. 114, 2432–2506 (2014).
Furuya, T., Kamlet, A. S. & Ritter, T. Catalysis for fluorination and trifluoromethylation. Nature 473, 470–477 (2011).
Yerien, D. E., Barata-Vallejo, S. & Postigo, A. Difluoromethylation reactions of organic compounds. Chem. Eur. J. 23, 14676–14701 (2017).
Rong, J., Ni, C. & Hu, J. Metal-catalyzed direct difluoromethylation reactions. Asian J. Org. Chem. 6, 139–152 (2017).
Lu, Y., Liu, C. & Chen, Q. Y. Recent advances in difluoromethylation reaction. Curr. Org. Chem. 19, 1638–1650 (2015).
Shah, P. & Westwell, A. D. The role of fluorine in medicinal chemistry. J. Enzym. Inhib. Med. Chem. 22, 527–540 (2007).
Martínez, M. D. et al. Antioxidant properties in a non-polar environment of difluoromethyl bioisosteres of methyl hydroxycinnamates. J. Pharm. Pharmacol. 68, 233–244 (2016).
Meanwell, N. A. Synopsis of some recent tactical application of bioisosteres in drug design. J. Med. Chem. 54, 2529–2591 (2011).
Sessler, C. D. et al. CF2H, a hydrogen bond donor. J. Am. Chem. Soc. 139, 9325–9332 (2017).
Caminati, W., Melandri, S., Moreschini, P. & Favero, P. G. The C-F···H-C “anti-hydrogen bond” in the gas phase: microwave structure of the difluoromethane dimer. Angew. Chem. Int. Ed. 38, 2924–2925 (1999).
Erickson, J. A. & McLoughlin, J. I. Hydrogen bond donor properties of the difluoromethyl group. J. Org. Chem. 60, 1626–1631 (1995).
Zafrani, Y. et al. Difluoromethyl bioisostere: examining the “lipophilic hydrogen bond donor” concept. J. Med. Chem. 60, 797–804 (2017).
Josephson, B. et al. Light-driven post-translational installation of reactive protein side chains. Nature 585, 530–537 (2020).
Zheng, B. et al. Potent inhibitors of hepatitis C virus NS3 protease: employment of a difluoromethyl group as a hydrogen-bond donor. ACS Med. Chem. Lett. 9, 143–148 (2018).
Rageot, D. et al. Discovery and preclinical characterization of 5-[4,6-Bis({3-oxa-8-azabicyclo[3.2.1]octan-8-yl})-1,3,5-triazin-2-yl]-4-(difluoromethyl)pyridin-2-amine (PQR620), a highly potent and selective mTORC1/2 inhibitor for cancer and neurological disorders.J. Med. Chem. 61, 10084–10105 (2018).
Naret, T. et al. A fluorine scan of a tubulin polymerization inhibitor isocombretastatin A-4: design, synthesis, molecular modelling, and biological evaluation. Eur. J. Med. Chem. 143, 473–490 (2018).
Rodriguez, C. R. et al. Synthesis and biological activity of fluorinated analogues of the DAF-12 receptor antagonist 24-hydroxy-4-cholen-3-one. Steroids 151, 108469 (2019).
Fujiwara, Y. et al. A new reagent for direct difluoromethylation. J. Am. Chem. Soc. 134, 1494–1497 (2012).
Zhu, S.-Q., Liu, Y.-L., Li, H., Xu, X.-H. & Qing, F.-L. Direct and regioselective C–H oxidative difluoromethylation of heteroarenes. J. Am. Chem. Soc. 140, 11613–11617 (2018).
Prakash, G. K. S. et al. Copper-mediated difluoromethylation of (hetero)aryl Iodides and β-styryl halides with tributyl(difluoromethyl)stannane. Angew. Chem. Int. Ed. 51, 12090–12094 (2012).
Gu, Y., Leng, X. & Shen, Q. Cooperative dual palladium/silver catalyst for direct difluoromethylation of aryl bromides and iodides. Nat. Commun. 5, 5405 (2014).
Xu, L. & Vicic, D. A. Direct difluoromethylation of aryl halides via base metal catalysis at room temperature. J. Am. Chem. Soc. 138, 2536–2539 (2016).
Serizawa, H., Ishii, K., Aikawa, K. & Mikami, K. Copper-catalyzed difluoromethylation of aryl iodides with (difluoromethyl)zinc reagent. Org. Lett. 18, 3686–3689 (2016).
Lu, C., Gu, Y., Wu, J., Gu, Y. & Shen, Q. Palladium-catalyzed difluoromethylation of heteroaryl chlorides, bromides and iodides. Chem. Sci. 8, 4848–4852 (2017).
Bour, J. R., Kariofillis, S. K. & Sanford, M. S. Synthesis, reactivity, and catalytic applications of isolable (NHC)Cu(CHF2) complexes. Organometallics 36, 1220–1223 (2017).
Xu, C. et al. Difluoromethylation of (hetero)aryl chlorides with chlorodifluoromethane catalyzed by nickel. Nat. Commun. 9, 1170 (2018).
Miao, W. et al. Iron-catalyzed difluoromethylation of arylzincs with difluoromethyl 2-pyridyl sulfone. J. Am. Chem. Soc. 140, 880–883 (2018).
Ferguson, D. M., Malapit, C. A., Bour, J. R. & Sanford, M. S. Palladium-catalyzed difluoromethylation of aryl chlorides and bromides with TMSCF2H. J. Org. Chem. 84, 3735–3740 (2019).
Pan, F., Boursalian, G. B. & Ritter, T. Palladium-catalyzed decarbonylative difluoromethylation of acid chlorides at room temperature. Angew. Chem. Int. Ed. 57, 16871–16876 (2018).
Fier, P. S. & Hartwig, J. F. Copper-mediated difluoromethylation of aryl and vinyl iodides. J. Am. Chem. Soc. 134, 5524–5527 (2012).
Matheis, C., Jouvin, K. & Goossen, L. J. Sandmeyer difluoromethylation of (hetero-)arenediazonium salts. Org. Lett. 16, 5984–5987 (2014).
Feng, Z., Min, Q.-Q., Fu, X.-P., An, L. & Zhang, X. Chlorodifluoromethane-triggered formation of difluoromethylated arenes catalysed by palladium. Nat. Chem. 9, 918–923 (2017).
Bacauanu, V. et al. Metallaphotoredox difluoromethylation of aryl bromides. Angew. Chem. Int. Ed. 57, 12543–12548 (2018).
Endo, Y., Ishii, K. & Mikami, K. Chiral copper-catalyzed enantioselective Michael difluoromethylation of arylidene meldrum’s acids with (difluoromethyl)zinc reagents. Tetrahedron 75, 4099–4103 (2019).
Tang, X.-J., Zhang, Z. & Dolbier, W. R. Jr Direct photoredox-catalyzed reductive difluoromethylation of electron-deficient alkenes. Chem. Eur. J. 21, 18961–18965 (2015).
Lin, Q.-Y., Xu, X.-H., Zhang, K. & Qing, F.-L. Visible-light-induced hydrodifluoromethylation of alkenes with a bromodifluoromethylphosphonium bromide. Angew. Chem. Int. Ed. 55, 1479–1483 (2016).
Zhang, M., Lin, J.-H. & Xiao, J.-C. Photocatalyzed cyanodifluoromethylation of alkenes. Angew. Chem. Int. Ed. 58, 6079–6083 (2019).
Meyer, C. F., Hell, S. M., Misale, A., Trabanco, A. A. & Gouverneur, V. Hydrodifluoromethylation of alkenes with difluoroacetic acid. Angew. Chem. Int. Ed. 58, 8829–8833 (2019).
Xu, C., Yang, Z.-F., An, L. & Zhang, X. Nickel-catalyzed difluoroalkylation–alkylation of enamides. ACS Catal. 9, 8224–8229 (2019).
Yang, J., Zhu, S., Wang, F., Qing, F.-L. & Chu, L. Silver-enabled general radical difluoromethylation reaction with TMSCF2H. Angew. Chem. Int. Ed. 60, 4300–4306 (2021).
Levi, N., Amir, D., Gershonov, E. & Zafrani, Y. Recent progress on the synthesis of CF2H-containing derivatives. Synthesis 51, 4549–4567 (2019).
Zeng, X. et al. Copper-catalyzed decarboxylative difluoromethylation. J. Am. Chem. Soc. 141, 11398–11403 (2019).
Zeng, X. et al. Copper-catalyzed deaminative difluoromethylation. Angew. Chem. Int. Ed. 59, 16398–16403 (2020).
Zeng, X. J. et al. Copper-catalyzed, chloroamide-directed benzylic C–H difluoromethylation. J. Am. Chem. Soc. 141, 19941–19949 (2019).
Ishii, T., Ota, K., Nagao, K. & Ohmiya, H. N-heterocyclic carbene-catalyzed radical relay enabling vicinal alkylacylation of alkenes. J. Am. Chem. Soc. 141, 14073–14077 (2019).
Qin, T. et al. A general alkyl-alkyl cross-coupling enabled by redox-active esters and alkylzinc reagents. Science 352, 801 (2016).
Zhang, X. et al. Copper-mediated synthesis of drug-like bicyclopentanes. Nature 580, 220–226 (2020).
Huang, H.-M., Garduño-Castro, M. H., Morrill, C. & Procter, D. J. Catalytic cascade reactions by radical relay. Chem. Soc. Rev. 48, 4626–4638 (2019).
Derosa, J., Apolinar, O., Kang, T., Tran, V. T. & Engle, K. M. Recent developments in nickel-catalyzed intermolecular dicarbofunctionalization of alkenes. Chem. Sci. 11, 4287–4296 (2020).
Zhang, J.-S., Liu, L., Chen, T. & Han, L.-B. Transition-metal-catalyzed three-component difunctionalizations of alkenes. Chem. Asian J. 13, 2277–2291 (2018).
Egami, H. & Sodeoka, M. Trifluoromethylation of alkenes with concomitant introduction of additional functional groups. Angew. Chem. Int. Ed. 53, 8294–8308 (2014).
Gu, Y., Chang, D., Leng, X., Gu, Y. & Shen, Q. Well-defined, shelf-stable (NHC)Ag(CF2H) complexes for difluoromethylation. Organometallics 34, 3065–3071 (2015).
DiMucci, I. M. et al. The myth of d8 copper(III). J. Am. Chem. Soc. 141, 18508–18520 (2019).
Paeth, M. et al. Csp3–Csp3 bond-forming reductive elimination from well-defined copper(III) complexes. J. Am. Chem. Soc. 141, 3153–3159 (2019).
Liu, S. et al. C(sp3)-CF3 reductive elimination from a five-coordinate neutral copper(III) complex. J. Am. Chem. Soc. 142, 9785–9791 (2020).
Zhang, W. et al. Enantioselective cyanation of benzylic C–H bonds via copper-catalyzed radical relay. Science 353, 1014–1018 (2016).
Gockel, S. N., Buchanan, T. L. & Hull, K. L. Cu-catalyzed three-component carboamination of alkenes. J. Am. Chem. Soc. 140, 58–61 (2018).
He, J., Chen, C., Fu, G. C. & Peters, J. C. Visible-light-induced, copper-catalyzed three-component coupling of alkyl halides, olefins, and trifluoromethylthiolate to generate trifluoromethyl thioethers. ACS Catal. 8, 11741–11748 (2018).
Wang, F., Chen, P. & Liu, G. Copper-catalyzed radical relay for asymmetric radical transformations. Acc. Chem. Res. 51, 2036–2046 (2018).
Xiao, H., Shen, H., Zhu, L. & Li, C. Copper-catalyzed radical aminotrifluoromethylation of alkenes. J. Am. Chem. Soc. 141, 11440–11445 (2019).
Zhang, Z., Zhu, L. & Li, C. Copper-catalyzed carbotrifluoromethylation of unactivated alkenes driven by trifluoromethylation of alkyl radicals. Chin. J. Chem. 37, 452–456 (2019).
Vitaku, E., Smith, D. T. & Njardarson, J. T. Analysis of the structural diversity, substitution patterns, and frequency of nitrogen heterocycles among U.S. FDA approved pharmaceuticals. J. Med. Chem. 57, 10257–10274 (2014).
Meerwein, H., Büchner, E. & van Emster, K. Über die einwirkung aromatischer diazoverbindungen auf α,β-ungesättigte carbonylverbindungen. J. f.ür. Praktische Chem. 152, 237–266 (1939).
Kindt, S. & Heinrich, M. R. Recent advances in meerwein arylation chemistry. Synthesis 48, 1597–1606 (2016).
Karatoprak, G.S. et al. Combretastatins: an overview of structure, probable mechanisms of action and potential applications. Molecules 25, 2560 (2020).
Paeth, M. et al. Copper-mediated trifluoromethylation of benzylic Csp3−H bonds. Chem. Eur. J. 24, 11559–11563 (2018).
Guo, S., AbuSalim, D. I. & Cook, S. P. Aqueous benzylic C–H trifluoromethylation for late-stage functionalization. J. Am. Chem. Soc. 140, 12378–12382 (2018).
Acknowledgements
W.L. thanks University of Cincinnati for financial support. Funding for the D8 Venture diffractometer was through NSF-MRI grant CHE-1625737. NMR experiments were performed using a Bruker AVANCE NEO 400 MHz NMR spectrometer (funded by NSF-MRI grant CHE-1726092). We also thank Prof. John T. Groves and Mr. Yuan Cao (Princeton University) for the assistance with the high-resolution mass spectroscopy.
Author information
Authors and Affiliations
Contributions
W.L. and A.C. conceived the project and designed the experiments. A.C., W.Y., X.Z. and S.Z. developed the reactions, and contributed to the reaction scope. J.K. solved the crystal structures. M.C. and T.C. performed the theoretical calculation. W.L. and A.C. wrote the paper, supplementary information, and related materials.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Peer review information Nature Communications thanks the anonymous reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are available.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Cai, A., Yan, W., Zeng, X. et al. Copper-catalyzed carbo-difluoromethylation of alkenes via radical relay. Nat Commun 12, 3272 (2021). https://doi.org/10.1038/s41467-021-23590-2
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41467-021-23590-2
- Springer Nature Limited
This article is cited by
-
Radical hydrodifluoromethylation of unsaturated C−C bonds via an electroreductively triggered two-pronged approach
Communications Chemistry (2022)