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No association of HLA–DRB1 and TNF alleles in Mexican patients with autoimmune hepatitis

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Abstract

HLA–DRB1 alleles has been found implicated in susceptibility to autoimmune hepatitis (AIH) in populations from different genetic backgrounds. In Mexicans, HLA–DRB1*04:04 is recognized as a risk allele for AIH but, to date, there is no high-resolution data supporting this association. Also, the association of other nonclassical HLA genes, such as TNF–LTA locus, have not, to our knowledge, been evaluated in this population. The association of HLA–DRB1 alleles determined by sequence-based typing and two polymorphisms in the TNF locus with AIH in a sample of Mexican patients was evaluated. Fifty-six patients from Guadalajara, Mexico, diagnosed with AIH and 115 age-gender matched healthy volunteer blood donors, were genotyped for HLA–DRB1 by the sequencing exon 2 and for TNFA–308G>A and LTA + 252A>G polymorphisms. Increased frequencies of both HLA–DRB1*04:04:01 and *16:02:01:01 alleles (OR = 2.91; 95% CI = 1.08–7.84) and the haplotype (DRB1–TNFA–LTA) *04:04:01-G-A (OR = 5.33; 95% CI = 1.32–21.49) were observed in AIH patients. However, after corrections for multiple comparisons, associations were not significant. In conclusion, our study does not support the association of HLA–DRB1*04:04:01 with the susceptibility to AIH in Mexican population. More studies including patients from other Mexican regions and considering other genetic, immunological, and environmental factors should be performed.

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Acknowledgements

This study was funded by Fondo de Investigación en Salud of IMSS, (grant number: FIS/IMSS/PROT/279. We thank Consejo Nacional de Ciencia y Tecnología (CONACyT) and IMSS for fellowships to XHC-M and LEH-R during their postgraduate studies.

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Authors and Affiliations

  1. Molecular Medicine Division, Centro de Investigación Biomédica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, Mexico

    Francisco Mendoza-Carrera & Luis Eduardo Hernández-Ramos

  2. Gastroenterology Service, Unidad Médica de Alta Especialidad Dr. Ignacio García Téllez, IMSS, Guadalajara, Jalisco, Mexico

    Miguel Ángel Gastélum-Meza, Jesús Ramírez-García & Carlos Dávalos-Cobián

  3. National Institute of Genomic Medicine (INMEGEN), Basic Research Area, Mexico City, Mexico

    Xochitl Helga Castro-Martínez

  4. Continuous Admission Service, Unidad Médica de Alta Especialidad Dr. Ignacio García Téllez, IMSS, Guadalajara, Jalisco, Mexico

    María Inmaculada Concepción Arellano-Olivera

  5. Surgical Research Division, CIBO, IMSS, Guadalajara, Jalisco, Mexico

    Caridad Leal-Cortés

Authors
  1. Francisco Mendoza-Carrera
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  2. Miguel Ángel Gastélum-Meza
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  3. Jesús Ramírez-García
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  4. Carlos Dávalos-Cobián
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  5. Xochitl Helga Castro-Martínez
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  7. Luis Eduardo Hernández-Ramos
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  8. Caridad Leal-Cortés
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Correspondence to Francisco Mendoza-Carrera.

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Mendoza-Carrera, F., Gastélum-Meza, M.Á., Ramírez-García, J. et al. No association of HLA–DRB1 and TNF alleles in Mexican patients with autoimmune hepatitis. Genes Immun 20, 678–683 (2019). https://doi.org/10.1038/s41435-019-0086-8

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