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Studies on Streptomyces sp. SN-593: reveromycin biosynthesis, β-carboline biomediator activating LuxR family regulator, and construction of terpenoid biosynthetic platform

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Abstract

Streptomyces represents an important reservoir for biologically active natural products. Understanding the biosynthetic mechanism and the mode of gene expression is important for enhanced metabolite production and evaluation of biological activities. This review provides an overview of biosynthetic studies investigating reveromycin and β-carboline biomediators that enhanced the production of reveromycin in Streptomyces sp. SN-593 through activation of the LuxR family regulator. Furthermore, based on the optimal expression of a pathway specific regulator controlling the mevalonate pathway gene cluster, Streptomyces sp. SN-593 was developed as a platform for terpenoid compounds mass production.

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  • 15 July 2022

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Acknowledgements

Regarding winning Omura Award 2021 from Society for Actinomycetes Japan, I appreciate Hiroyuki Osada (RIKEN), Haruo Ikeda (Kitasato University), Jun Ishikawa (NIID), Atsushi Toyoda (NIG), Masakazu Uramoto (RIKEN), Tohru Dairi (Hokkaido University), Yasuyo Sekiyama (RIKEN), Hiroyuki Koshino (RIKEN), Shingo Nagano (Tottori University), Toshihiko Nogawa (RIKEN), Joe Chappell (University of Kentucky), Shigeru Okada (University of Tokyo), and many of whom I could not list here. The research was supported by JSPS KAKENHI grant (18580085, 21580104, 24380052, 24658088, 25108726, 16H04905 17H05455, 19H04666, 20H00416), JSBBA Innovative Research Program Award, and Nagase Foundation Award.

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  1. Natural Product Biosynthesis Research Unit, RIKEN Centre for Sustainable Resource Science, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Shunji Takahashi

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Dedication: The discovery of reveromycin was accomplished in Dr. lsono’s laboratory. I mourn his passing and dedicate this review to him.

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Takahashi, S. Studies on Streptomyces sp. SN-593: reveromycin biosynthesis, β-carboline biomediator activating LuxR family regulator, and construction of terpenoid biosynthetic platform. J Antibiot 75, 432–444 (2022). https://doi.org/10.1038/s41429-022-00539-1

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