Abstract
Premature ovarian failure and female infertility are frequent side effects of anticancer therapies, owing to the extreme sensitivity of the ovarian reserve oocytes to the damaging effects of irradiation and chemotherapy on DNA. We report here a robust protective effect of luteinizing hormone (LH) on the primordial follicle pool of prepubertal ovaries against the cisplatin (Cs)-induced apoptosis. In vitro LH treatment of prepubertal ovarian fragments generated anti-apoptotic signals by a subset of ovarian somatic cells expressing LH receptor (LHR) through cAMP/PKA and Akt pathways. Such signals, reducing the oocyte level of pro-apoptotic TAp63 protein and favoring the repair of the Cs-damaged DNA in the oocytes, prevented their apoptosis. Noteworthy, in vivo administration to prepubertal female mice of a single dose of LH together with Cs inhibited the depletion of the primordial follicle reserve caused by the drug and preserved their fertility in reproductive age, preventing significant alteration in the number of pregnancy and of delivered pups. In conclusion, these findings establish a novel ovoprotective role for LH and further support the very attracting prospective to use physiological 'fertoprotective' approaches for preventing premature infertility and risks linked to precocious menopause in young patients who survived cancer after chemotherapy.
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In mammals, the growing follicles develop from a pool of primordial follicles constituted early in life. Despite a current lively debate,1 such follicles, termed the ovarian reserve, represent probably the only pool available for ovulation in the mammalian females during their entire reproductive life.
Premature ovarian failure and infertility are routine side effects of anticancer therapies, owing to the extreme sensitivity of the ovarian reserve oocytes, mainly to the DNA-damaging effects of irradiation (IR) and chemotherapic drugs.2, 3
At present, methods to preserve future fertility in cancer patients are invasive and in some cases still at experimental level.4 The development of chemotherapic agents that do not harm the ovarian reserve, or of substances capable of protecting oocytes from the deleterious effects of such drugs ('fertoprotective agents') would represent a major improvement to preserve fertility in women.5
Several laboratories have recently investigated the molecular pathways involved in the death of oocytes exposed to IR or chemotherapic drugs. According to the current model, the alpha TAp63 isoform, found in the nucleus of oocytes, is a key mediator of the DNA damage response in the primordial follicle oocytes.6, 7, 8, 9, 10 Studies from our and other laboratories have indicated that the co-treatment with c-ABL inhibitors has a significant protective effect on the ovarian reserve challenged by cisplatin (Cs), a chemotherapic drug used to treat different types of cancer, likely preventing TAp63 activation.3, 11 Accordingly, other reports showed that TAp63 activation increases the expression of PUMA and NOXA, causing oocyte apoptosis12 and that pharmacological inhibition of c-ABL impinges on an integrated p53 family-signaling network (involving both TAp63 and TAp73), reducing BAX/BAK-mediated oocyte apoptosis.13
Other compounds, such as sphingosine-1-phosphate (S1P) or the S1P mimetic FTY720,14, 15, 16 dexrazoxane,17 AS101,18 sildelnafil citrate,19 tamoxifen,20 have also been shown to act as primordial follicle protective agents after IR and/or chemotherapy.
Administration of gonadotropin-releasing hormone agonists (GnRHa) during chemotherapy has been reported to be beneficial for fertility preservation in adolescent mammalian females,21, 22, 23 opening the exiting possibility of using biological molecules with targeted action. However, the effectiveness and mechanisms of action of GnRHa, in protecting the ovarian reserve from chemotherapy-induced damage, is still debated.24, 25, 26
In the present work, we hypothesized that luteinizing hormone (LH) and follicle-stimulating hormone (FSH) may exert a protective action on the oocytes of the ovarian reserve against apoptosis induced by Cs. The physiological functions of these gonadotropins are well delineated in the adult.27, 28, 29, 30 Much less characterized is the function that LH and FSH may exert on the early stages of folliculogenesis. Earlier studies in humans have demonstrated that FSH receptors are expressed in follicles from primary to later stages31 and treatment with FSH and LH promotes preantral follicle growth.32, 33 In differentiating rodent gonads, a truncated LH receptor (LHR) mRNA can be detected even before gonad formation, whereas the first full-length LHR has been detected in rat and mouse ovaries at 5 days post partum.34, 35
In vitro and in vivo results reported here show that LH, and at lesser extent FSH, has the ability to protect the primordial follicle pool present in the ovaries of prepuberal mice against Cs-induced apoptosis of, thereby preserving their future fertility in the reproductive age.
Results
LH protects POs from degeneration induced by Cs in culture
Ovaries from GFP-cKit transgenic mice of 4 days post partum (P4) were cut into small fragments and cultured for 4 days in order to allow spreading of ovarian somatic cells and facilitate the score of the fluorescent oocytes under the microscope; cultures were then exposed for 24 h to two different doses of Cs. We found that the number of the primordial follicle-enclosed oocytes (diameter <20 μm, hereafter termed as primordial oocytes, POs) was severely reduced in the ovarian fragments, incubated in the presence of Cs in comparison with untreated cells (Figures 1a and b) (% morphologically healthy POs, mean±S.E.: Ctrl = 96 ± 1.2; 7.5 μM Cs=54±3.8; 10 μM Cs=33±1.1). Differently, we observed that the few primary/secondary follicle-enclosed oocytes (hereafter termed as growing oocytes, GOs) were unaffected by the Cs treatment (Figure 1a). In order to determine whether these results were due to a stage-dependent oocyte sensitivity to Cs, we repeated the same experiments on POs and GOs, isolated from P7 to P8 ovaries and freed from follicle cells. The results showed that while only a few denuded POs survived after 24 h in the presence of 10 μM Cs (35/155=22.6%), the large majority of GOs (56/60=93%) remained healthy as estimated both morphologically and by the Trypan blue test (Supplementary Figure 1A and data not shown). Thus, Cs was able to directly induce death in primordial but not GOs in vitro.
Strikingly, we found that pretreatment with LH, in the range of 50–200 mIU/ml (about 0.6–2.3 ng/ml) 1 h before Cs addition, favored in a dose-dependent manner the survival of POs within the ovarian fragments after 24 h of culture, approaching their number to that of the drug-untreated samples (% healthy POs: 7.5 μM Cs±200 mIU LH=80±3.6; 10 μM Cs+200 mIU LH=68±3.0) (Figures 1a and b and Supplementary Figure 1B). Noteworthy, LH did not prevent degeneration of POs cultured either freed from pregranulosa cells (data not shown) or enclosed in the follicles isolated from ovarian fragments (Supplementary Figure 1C).
Interestingly, we found that LH was also able to sustain the survival of the ovarian somatic cells that was clearly impaired by Cs treatment, as evaluated by morphological observation and the Trypan blue assay (Supplementary Figure 1D). Overall, these results suggest that LH ovoprotection was actually indirect and mediated by a subset of ovarian somatic cells.
Finally, we found that also FSH had a slight albeit statistically significant protective effect on the Cs ovotoxicity within the ovarian fragments (Figure 1c), but not on POs within isolated follicles, as described with LH (Supplementary Figure 1C).
As LH appeared to exert a significantly much higher ovoprotection than FSH, we focused the subsequent experiments on the LH action.
A subset of the somatic cells of early postnatal ovaries express LHR
Western blotting analyses proved that the LHR was expressed at appraisable level both in postnatal ovaries as early as P2 and in the P4 ovarian fragments cultured for 4 days (Figure 2a). RT-PCR analysis confirmed full-length LHR transcripts in the ovarian somatic cells but not in denuded oocytes obtained from P4 ovaries (Figure 2b). In adult and P8 ovaries, LHR was immunolocalized in cells surrounding the growing follicles (presumably theca cells) (Figure 2c). In P4 ovarian fragments cultured for 4 days, a significant number of LHR-positive cells appeared to surround the region containing the primordial and growing follicles (Figure 2c).
LH ovoprotection against Cs is mimicked by cAMP increasing agents and requires PKA and AKT activities
cAMP is the canonical second messenger of LH.36 In line with this notion, we found that LH was able to significantly increase the level of cAMP in the ovarian tissue fragments used in our in vitro culture assay (Supplementary Figure 2). Moreover, we found that the addition of 8Br-cAMP (a cell permeable analog of cAMP) to the cultured fragments partly replaced the LH action and that forskolin (FRSK), a potent activator of adenylate cyclase, completely substituted the LH ovoprotective effect (Figure 3a).
Interestingly, FRSK exerted robust ovoprotection against Cs also on isolated follicle-enclosed and denuded POs (% healthy follicle-enclosed POs: 10 μM Cs=15±2.6 and 10 μM Cs+10 μM FRSK=58±3.5; % healthy denuded POs: 10 μM Cs=17±5 and 10 μM Cs+10 μM FRSK=51.4±9), thus suggesting that high levels of cAMP in the oocyte exerts a protective action against Cs toxicity. In line with this, H89, a specific inhibitor of cAMP-dependent protein kinase (PKA), significantly reduced the LH effect (Figure 3a).
Recent data emphasized that LH, beside PKA activation, may stimulate other signaling pathways, including AKT and ERK kinase activities.37 In this respect, we found that two specific inhibitors of PI3K-dependent AKT phosphorylation/activation, LY294002 and wortmannin, abolished the protective effect of LH on the POs within the drug-treated ovarian fragments (Figure 3b). The finding that incubation of P7–8 ovaries with LH resulted in increased AKT phosphorylation, further supported the concept that LH ovoprotection involves AKT activation (Figure 3c).
Conversely, ERK1/2 kinase activation did not appear to be involved in the LH action in our cell system. In fact, we found that treatment with Cs alone increased ERK1/2 phosphorylation/activation in the P7–8 ovaries and that LH did not significantly affect such increase nor the basal ERK1/2 activity (Figures 3d and e). Moreover, U0126 (a specific inhibitor of the MEK1-dependent ERK activation) reduced Cs ovotoxicity in the ovarian fragments, preventing the increased ERK1/2 activity associated to Cs action (see also Figure 3d), without, however, influencing the ovoprotective action of LH (Figure 3f).
LH ovoprotection against Cs requires functional gap junctions and can be partly replaced by KL
As communications through gap junctions among follicular cells and between follicular cells and the oocyte have a crucial role in the functional interactions that regulate oogenesis,38 we investigated whether the LH ovoprotection against Cs required functional gap junctions. To this aim, we added to the cultured ovarian tissues carbenoxolone (Cx), a prototype gap junction blocker, at a concentration of 100 μM.39 Cx did not affect the oocyte survival in control conditions nor influenced the Cs ovotoxicity whereas it completely abolished the LH ovoprotection against the drug (Figure 4a).
LH has been shown to increase KL expression in granulosa and/or theca cells in the adult ovary.40 In line with these results, we found that LH stimulation increased KL expression in the P4 ovarian tissue fragments cultured for 4 days and in P8 ovaries as well (Figure 4b). Moreover, we found that in our culture assay the addiction of KL exerted significant ovoprotection against Cs (Figure 4c). Finally, the cytokine exerted ovoprotection both on isolated follicle-enclosed and denuded POs (% healthy primordial follicle-enclosed POs: 10 μM Cs=15±2.6; 10 μM Cs+100 ng/ml KL=47±0.7; % healthy denuded POs: 10 μM Cs=22±8.0; 10 μM Cs+100ng/ml KL=46±3.4).
The possibility that LH exerted its ovoprotection on the POs through specific factors produced by ovarian somatic cells was next investigated by culturing isolated primordial follicles onto monolayers obtained from ovaries of P7–8 or of the MCF-7 cells (a human breast adenocarcinoma cell line) expressing LHR (Figure 4d). We found that the addition of LH resulted effective only when follicles were co-cultured onto the ovarian cells, but not onto MCF-7 monolayers.
LH reduces TAp63 levels in Cs-treated oocytes
Previous studies by us and others have identified TAp63, specifically expressed by oocytes, as a key mediator of POs apoptosis, following IR or Cs treatment. We then investigated whether LH stimulation modulated TAp63 activity and apoptosis in the oocytes exposed to Cs.
Actually, LH was able to reduce TAp63 protein levels as well as caspase 3 activation in POs from P4 ovaries following Cs exposure (Figures 5a and b and Supplementary Figures 3A and 3B).
LH and FRSK favor DNA repair in Cs-treated oocytes
Citotoxic effects of Cs are largely mediated by DNA damage. In order to detect DNA damage in the POs, we used antibodies against γH2AX, a marker of DNA double-strand breaks. By IF, we observed that while in the control ovarian fragments γH2AX-positive POs were virtually absent throughout the 24-h culture period, their number markedly increased after 12 h of Cs treatment (36%±5.7), reaching 66%±6.4 in the few POs survived after 24 h. On the other hand, although the initial number of γH2AX-positive POs over the first 12 h after Cs treatment was not affected by LH, <5% of the POs remained γH2AX positive at 24 h in the presence of the hormone (Figures 5c and d). These results indicate that Cs causes DNA breaks in the POs irrespective of the presence of LH and suggest that most of the DNA breaks were repaired in the surviving oocytes after 24 h in the presence of the hormone. As shown in Figure 5e, Cs-treated POs underwent a similar DNA repair kinetics in the presence of FRSK. Differently, in the presence of KL the most part of the surviving Cs-treated oocytes appeared still γH2AX positive, after 24 h of culture (Figure 5e), suggesting that cAMP and KL generating signals prevent PO apoptosis by different mechanisms.
Staining of γH2AX-positive ovarian somatic cells was significantly prevented by incubation with LH and Cs as compared with cells treated with Cs alone, suggesting that in ovarian somatic cells LH is able to prevent the DNA damage caused by the drug rather than to favor DNA repair machinery (Supplementary Figures 4a and b).
LH preserves ovarian reserve and fertility after Cs treatment
In order to determine whether LH was also effective in protecting the POs pool of the ovarian reserve in vivo, P4–5 mice were divided in four experimental groups and injected with physiological solution (control), LH, Cs or LH+Cs. We found that 5 days after treatment, the number of primordial follicles, determined by the analysis of histological sections, was drastically reduced in the group treated with Cs alone but not in that co-treated with LH, indicating a robust protective effect of LH on the ovarian reserve (Figures 6a and b).
Next, females of each group were let to grow and analyzed for their reproductive capacity, monitoring their mating capability and the number of pups delivered during seven breeding rounds. We observed that fertility was progressively impaired in females injected with Cs at the prepubertal age, whereas no significant alterations in the number of pregnancy and delivered pups were found in the LH+Cs group in comparison with control (Figures 6c and d). In addition, no evident differences in development and behavior were observed between the newborns from the two groups during the first postnatal week. The gross morphology and the analysis of histological sections of the ovaries at the end of the mating experiments, confirmed the nearly total absence of follicles in the Cs-treated females and normal ongoing folliculogenesis in control as well as in LH+Cs groups (Figures 6e and f).
Discussion
In this article, we focused on the possibility to use gonadotropins with targeted action on the ovary as 'fertoprotective agents' against a widely used chemotherapic and gonadotoxic drug as Cs. Our in vitro assay was performed on prepubertal ovaries, which contain almost only primordial follicles, representing the ovarian reserve. We demonstrated that LH at physiological concentrations (around 2.3 ng/ml), preserved most of the POs against Cs-induced apoptosis. Furthermore, the protective effect of LH against Cs on PO in vitro was reproducible in vivo, on P4 mice, that have an immature hypothalamic–pituitary–gonad axis and therefore ovaries not exposed to the LH concentrations used in our assay. As expected, as oocytes do not express LHR, the protection was indirect and mediated by the ovarian somatic cells. The pregranulosa cells surrounding isolated primordial follicle appeared alone unable or insufficient to protect the oocyte from Cs following LH incubation. Thus, indicating that LH ovoprotection requires signaling from different or more numerous pregranulosa cells.
The apparent resistance to Cs of the GOs present in the ovarian tissues in our assay is in line with similar observations.3, 7, 8, 41 The reason of insensitivity was not investigated, but it could be due progressive loss of the pro-apoptotic TAp63 expression from the late primary oocyte stage onward.3, 7, 8, 42
LH, upon activation of its receptor, can modulate cAMP/PKA and PI3K/AKT signaling;37 accordingly, we found that LH increased the level of cAMP and the AKT activity in ovarian fragments and PKA or PI3K inhibitors abolished LH protective effects. Moreover, compounds that mimic or increase intracellular cAMP such as 8Br-cAMP and FRSK, respectively, were able to partly or completely replace LH in its ovoprotective effect.
Several studies have indicated that the Kit ligand (KL), produced by granulosa cells, and its receptor Kit, expressed by theca cells and oocytes, exert crucial roles during early oogenesis, including anti-apoptotic effect on primordial follicle and oocyte survival, relevant in the present framework.43, 44 Accordingly to previous papers,40 we found that LH stimulation increased KL expression and that KL was able to replace the ovoprotection exerted by LH against Cs in the ovarian tissues and fragments.
Whether LH ovoprotection finally results in the activation of cAMP/PKA and PI3K/AKT pathways in the oocytes remains to be investigated. Notwithstanding, it appeared to require the maintenance of functional gap junctions in the ovarian tissues, as it did not occur in the presence of a gap junction blocker. It can be speculated that following LH stimulation, ovarian cells exchange among them and/or pass to the oocytes through gap junctions signals (i.e., cAMP) and metabolites necessary for an effective response against the Cs-induced cytotoxicity. In this regard, others studies have demonstrated that in the adult ovary, LH stimulates the meiotic resumption of the fully growth oocytes both by triggering the closure of gap junction among the LHR-expressing follicular cells and a positive stimulus passing through the gap junctions between the cumulus cells and the oocyte.45, 46
Considering that POs express high level of the KL receptor Kit and the well-known anti-apoptotic effect of the KL/Kit system on such oocytes,35, 44 it appears likely that besides through gap junctions, the LH-responsive ovarian somatic cells can send anti-Cs cytotoxicity signals to POs via such system.
FRSK and KL are shown here to counteract Cs-induced oocyte death also on isolated follicle-enclosed and denuded POs. These results indicate that PKA and PI3K/AKT activities, this latter known to be downstream the activation of Kit,47, 48 are crucially involved in the internal molecular mechanisms of the oocytes, saving them from Cs. It remains to define whether cAMP passing through the gap junctions and KL produced by the ovarian cells surrounding the oocytes, are actually the direct and only mediators of the LH ovoprotective effect in our system.
The experiments, aimed to elucidate the oocyte response mechanisms against Cs underlying LH ovoprotection, indicated that these impinge on reducing TAp63 levels and favoring the repair of DNA damage occurring as a consequence of Cs treatment. These latter results are particularly relevant because they suggest that oocytes surviving in the presence of the hormone, not only are protected from apoptosis but do not carry permanent DNA damage. The observation that FRSK determined DNA repair kinetics in oocytes similar to LH suggest that the DNA repair in oocytes can be modulated by PKA dependent signaling.
The observed different kinetics of DNA damage and repair in oocytes following Cs treatment in the presence of FRSK and KL imply that while stimulation of PKA activity saves oocytes from apoptosis favoring/accelerating DNA repair, PI3K/AKT promotes oocytes survival though distinct mechanisms, involving anti-apoptotic cytoplasmic response. Whereas the cAMP signaling system was reported to have important role in modulation of DNA damage repair pathways, its action can be either favoring or inhibiting such pathways depending on the cell type and activity.49, 50, 51 On the other hand, it is known that the anti-apoptotic PI3K/AKT effect can target both DNA repair and pro-survival cytosolic pathways.52 Interestingly, the kinetics of the γH2AX DNA foci in the ovarian somatic cells showed that in these cells LH prevented the bulk of the DNA breaks generated by Cs rather than favor their repair.
GnRH agonist administration is considered among the strategies to preserve ovarian function in adolescent patients treated with certain chemotherapy drugs, although its efficacy and mechanisms of action is still debated.25, 26, 53, 54 Several groups reported that GnRH agonist-treated cancer patients resumed cyclic ovarian function, whereas patients in the chemotherapy alone group experienced amenorrhea.55, 56 Ataya et al.21 showed that GnRH agonists administrated in parallel with cyclophosphamide significantly decreased the daily rate of follicular decline and the total number of primordial follicles lost during the chemotherapeutic insult. Furthermore, it has been recently shown that GnRHa co-treatment with Cs, induced, in human ovarian cortical samples, an increase of cAMP levels,54 although no protection was seen. Our findings highlight the role of LH-induced cAMP in follicle cells for PO protection after gonadotoxic treatments and support a possible direct role of GnRH agonist on the prepuberal ovary, in the preservation of ovarian function in young cancer patients.
In conclusion, we report a novel unexpected effect of LH on mouse primordial follicles of prepubertal ovaries, resulting in robust protection of the oocytes against Cs-induced apoptosis and preserving fertility at adult age. Our results indicate that at this early developmental stage, the ovary is responsive to the hormone and that LH-responsive cells may send signals to the primordial follicle-enclosed oocytes able to modulate their response to DNA-damaging compounds. These findings beside to develop new concepts about the role of LH in folliculogenesis and the DNA repair mechanisms of POs, may drive the development of a more physiological 'fertoprotective' strategy for young female cancer patients.
Materials and Methods
Animals
CD-1 and transgenic p-18 GFP/c-Kit mice57 were housed and mated under standard laboratory conditions in an environmentally controlled room and treated using humane care in order to inflict the least possible pain. All experiments were approved by the Institutional Animal Care and Use Committee (IACUC) and carried out according to the Italian and European rules (D.L.116/92; C.E. 609/86; European Directive 2010/63/EU).
Compounds and antibodies
The following compounds have been used: Cs, wortmannin, H89, LY294002, U0126, 8-Bromo-cAMP, FRSK, carboxenolone (all from Sigma-Aldrich, Gallarate, Italy), human highly purified recombinant LH and human highly purified recombinant FSH (Merck-Serono, Rome, Italy), recombinant human KL and EGF (Immunological Sciences, Rome, Italy).
The following primary antibodies were used: p63 (4A4-Santa Cruz, Santa Cruz, CA, USA), tubulin (T9026 Sigma-Aldrich), AKT (Biosource, San Diego, CA, USA), phospho-AKT (Immunological Science), γH2AX (ser 139 Millipore, Vimodrome, MI, Italy), LHR (H-50 Santa Cruz), caspase 3 (9662 Cell Signaling, Danvers, MA, USA), actin (A2066 Sigma-Aldrich).
Ovary culture
Ovarian tissues were cultured as described in Maiani et al.13 Briefly, ovaries were collected from P4 transgenic p-18 GFP/c-Kit mice, in which all oocytes, expressing c-Kit, are GFP positive. Each ovary was sliced into 7–8 pieces and cultured in α-MEM (Aurogene, Rome, Italy), supplemented with 10% FBS (Gibco, Waltham, MA, USA), l-glutammine, penicillin-G and streptomycin, pyruvic acid, N-acetyl-l-cysteine and ITS liquid media supplement (100x), hereafter termed as CM, (all from Sigma-Aldrich), for 4 day at 37 °C in 5% CO2. At this time, when the ovarian fragments formed a thin layer of tissue containing follicles with visible oocytes under a fluorescence inverted microscope, the number of morphologically healthy GFP-positive oocytes with a diameter <20 μm (POs) was scored (T0 time point) and drugs were added to the culture medium at the indicated concentrations for further 24 h. Oocyte morphology and GFP positivity were analyzed with a Leica CTR 6000 microscope (Watzlar, Germany) at approximately 300X.
Oocyte and primordial follicles culture
Ovaries from P5 CD-1 or p-18 GFP/c-Kit mice were mechanically fragmented and incubated in 0.5 mg/ml type IV collagenase (Sigma-Aldrich) for 1 h at 37 °C, followed by 30 min at 37° C in 40 mM EDTA+DNase. After mechanical disaggregation, cells were dispersed and the cell suspension cultured for 6 h in CM at 37 °C in 5% CO2. Oocytes floating onto the somatic cell monolayer were collected with a mouth-operated pipette, divided into experimental groups according to the diameter (< or>20 μM) and cultured in 24-wells in CM for 24 h at 37 °C in 5% CO2.
Ovaries from P5 CD-1 or p-18 GFP/c-Kit mice were mechanically fragmented and incubated in 1 mg/ml type I collagenase (Sigma-Aldrich) for 1 h at 30 °C in continuous shaking, followed by mechanical disaggregation. Cell suspension was then filtered through a 40 μm filter (Corning, New York, NY, USA) to remove undispersed tissue fragments,58 divided into experimental groups and cultured (approximately 200 follicles per well) in CM for 24 h at 37 °C in 5% CO2.
In co-culture experiments, primordial follicles were cultured for 24 h at 37 °C in 5% CO2 onto monolayers of ovarian somatic cells or MCF-7 cell line (from ATCC, Milan, Italy) up to 70% confluence in 24-wells culture plates. Ovarian cell monolayers were obtained from ovaries treated as described in the isolated oocyte culture section.
Immunofluorescence
At the indicated culture times, medium was removed and ovarian fragments were fixed in 4% paraformaldehyde (PFA) for 20 min at RT, permeabilized with PBS – 0.01% Triton for 10 min and blocked in 3% BSA 20 min at RT.
For γH2AX foci staining, fragments were incubated overnight at 4 °C with mouse γH2AX antibody in PBS-T (PBS+0.3% BSA+0.01% Triton). Following three 5- min PBS washes, primary antibody was detected with goat anti-mouse antibody (Alexa 568, Molecular Probes, Milan, Italy). Fragments where then incubated with Hoechst, mounted in PBS and glycerol and analyzed by Leica CTR 6000 microscope.
For LHR immunelocalization both in ovarian fragments and ovaries, samples were fixed in 4% PFA and the ovaries sectioned (5 μm sections) following standard histological procedures. Both fragments and ovarian sections were incubated overnight at 4 °C with LHR antibody (Santa Cruz) in PBS-T. Following three PBS washes of 5 min, primary antibody was detected with goat anti-rabbit antibody (Alexa 568). Fragments and sections where then incubated with Hoechst, mounted in PBS and glycerol and analyzed by Leica CTR 6000 and confocal laser scanning LSM 700 microscope. For negative controls, primary antibodies were omitted from the staining procedure.
Western blotting
Samples were lysed in 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.5% NP-40, 5 mM EDTA, 0.5% sodium deoxycholate, 1 mM PMSF, 1 mM sodium o-vanadate, 10 μg/ml leupeptine, 10 μg/ml aprotinin supplemented with protease inhibitors (all from Sigma-Aldrich). Samples were homogenized by ultrasonic homogenization and proteins concentration was determined by Bradford assay.
Aliquots of total protein extracts from samples after different treatments were resolved on SDS-polyacrylamide gel and transferred to a PVDF transfer membrane (GE Healthcare, Milan, Italy). Blots were blocked in 5% non-fat dry milk in PBS-T (PBS+0.05% Tween 20) for 1 h at RT and then incubated with primary antibodies o. n. at 4 °C in 1% milk in PBS-T. Membranes were incubated with HRP-conjugated secondary antibody for 1 h at RT and signals were detected by ImmunoCruz western blotting luminol reagent (Santa Cruz). Immunoblots were quantitatively evaluated using ImageJ software (NIH, Bethesda, MD, USA). Signal intensities of phospho-proteins were normalized to the corresponding protein signals.
RT-PCR
RNA was extracted from total ovary using RNeasy micro kit (Qiagen, Milan, Italy), according to the manufacturer’s instructions. c-DNA was synthesized from 1 μg of RNA by QuantiTect reverse transcription Kit. RT-PCR analysis was performed using Sigma-Aldrich primers:
LHCGR fwd: 5′-GCATCTGTAACACAGGCATCCG-3′
LHCGR rev: 5′-CGATGAGCGTCTGAATGGACT-3′
KL fwd: 5′-GAATCTCCGAAGAGGCCAGAAACTAGATCCTTT-3′
KL rev: 5′-CGTCCACAATTACACCTCTTGAAATTCTCTCTC-3′
GAPDH fwd: 5′-AACTTTGGCATTGTGGAAGG-3′
GAPDH rev: 5′-CCGTGTTCCTACCCCCAATGTG-3′.
Drugs treatment of animals
P5 CD-1 female mice (n=70; 14 per experimental group) were injected intraperitoneally once with 30 μl of physiological solution or equal volume of Cs (10 μM per mouse), LH (200 mIU per mouse) or Cs+LH solutions.
Histology
Five days after treatment, four mice were killed and ovaries collected for standard histological procedures and follicle counts in 5 μm sections stained with H&E. The type and the total number of follicles per ovary were estimated as described in Tilly.59 Briefly, follicles were counted in every fifth section throughout the entire ovary and the mean total number of follicle per section multiplied by the total number of sections of the ovary. All primordial and primary follicles were counted, whether or not an oocyte nucleus was visible. For growing follicle, only follicles with a visible oocyte nucleus were counted to prevent double counting.
cAMP ELISA
cAMP concentration was evaluated using the direct cAMP ELISA kit (ENZO, Rome, Italy) according to the manufacturer's instructions. Briefly, ovarian tissues obtained from two ovaries of P4 were cultured for 4 days; after incubation for the indicated times (see Supplementary Figure S2), with or without 200 mIU LH tissues were lysed with 100 μl of 0.1 M HCl. After centrifugation at 600 g for 5 min, the supernatant was treated with the kit reagents and analyzed with a Infinite F50 Tecan (Männedorf, Switzerland).
Mating protocol
Five weeks after treatment, female mice were randomly mated with males of proven fertility (one male to two females). Each experimental group (Control, LH, Cs, Cs+LH) consisted of a total of 10 females. Mating was established by the formation of the fertilization plug (within a week of continuous).21 All females were then separated from males and those plugged allowed to progress pregnancy until delivery (about 3 weeks). The mothers remained with their pups 1 week before separation. After an additional week (without breast-feeding), all females (previously plugged and unplugged) were mated again. Such mating round was repeated seven times for a total period of about 9 months. The number of pregnancies and pups was counted after each round. At the end of the experiments, ovaries were collected and analyzed with standard histological procedures in paraffin tissue sections.
Statistical analysis
Data were collected from at least three different experiments and analyzed with Graph-pad Prism 6.0 software (La Jolla, CA, USA). P-value was determined by one-way Anova followed by Bonferroni analyses comparing all groups of data. Statistical significance was based on P value: *<0.05, **<0.001, ***<0.0001.59
Abbreviations
- LH:
-
luteinizing hormone
- Cs:
-
cisplatin
- LHR:
-
luteinizing hormone receptor
- IR:
-
irradiation
- FSH:
-
follicle-stimulating hormone
- GnRHa:
-
gonadotropin-releasing hormone agonists
- POs:
-
primordial oocytes
- GOs:
-
growing oocytes
- FRSK:
-
forskolin
- Cx:
-
carbenoxolone
- KL:
-
Kit ligand
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Acknowledgements
This work was financially supported by Merck-Serono SPA, Italy, an affiliate of Merck KGaA, Darmstadt, Germany. We thank Mr. Gabriele Rossi for the preparation of the histological sections.
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Rossi, V., Lispi, M., Longobardi, S. et al. LH prevents cisplatin-induced apoptosis in oocytes and preserves female fertility in mouse. Cell Death Differ 24, 72–82 (2017). https://doi.org/10.1038/cdd.2016.97
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DOI: https://doi.org/10.1038/cdd.2016.97
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