Abstract
Although most programmed cell death (PCD) during animal development occurs by caspase-dependent apoptosis, autophagy-dependent cell death is also important in specific contexts. In previous studies, we established that PCD of the obsolete Drosophila larval midgut tissue is dependent on autophagy and can occur in the absence of the main components of the apoptotic pathway. As autophagy is primarily a survival mechanism in response to stress such as starvation, it is currently unclear if the regulation and mechanism of autophagy as a pro-death pathway is distinct to that as pro-survival. To establish the requirement of the components of the autophagy pathway during cell death, we examined the effect of systematically knocking down components of the autophagy machinery on autophagy induction and timing of midgut PCD. We found that there is a distinct requirement of the individual components of the autophagy pathway in a pro-death context. Furthermore, we show that TORC1 is upstream of autophagy induction in the midgut indicating that while the machinery may be distinct the activation may occur similarly in PCD and during starvation-induced autophagy signalling. Our data reveal that while autophagy initiation occurs similarly in different cellular contexts, there is a tissue/function-specific requirement for the components of the autophagic machinery.
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Main
There is a fundamental requirement for multicellular organisms to remove excess, detrimental, obsolete and damaged cells by programmed cell death (PCD).1, 2 In the majority of cases caspase-dependent apoptosis is the principle pathway of PCD; however, there are other modes of cell death with important context-specific roles, such as autophagy.3, 4 Defects in autophagy have significant adverse consequences to normal cellular functions and contribute to the pathogenesis of numerous human diseases. This is particularly evident in cancer where depending on the context autophagy can have tumour-suppressing or -promoting roles. Given the number of clinical trials targeting autophagy in cancer therapy, it will be critically important to understand the context-specific regulation and functions of autophagy.5
Autophagy is a highly conserved multi-step catabolic process characterised by the encapsulation of part of the cytoplasm inside a double-membrane vesicle called the autophagosome. Autophagosomes then fuse with lysosomes and the components are subsequently degraded by acidic lysosomal hydrolases.6 The process of autophagy can be functionally divided into four groups: (1) serine/threonine kinase Atg1 (ULK1 in mammals) complex and its regulators responsible for the induction of autophagy; (2) the class III phosphatidylinositol 3-kinase (PI3K) complex, which involves Atg6 and functions in the nucleation of the autophagosome; (3) the Atg8 and Atg12 conjugation systems, which involves several Autophagy-related (Atg) proteins essential for the expansion of autophagosome; and (4) Atg9 and its associated proteins including Atg2 and Atg18, which aids the recycling of lipid and proteins.7 In addition, several of the Atg proteins can function in multiple steps. For example, Atg1 interacts with proteins with different functions (e.g. Atg8, Atg18 and others), suggesting that it is not only required for initiation but also participates in the formation of autophagosomes.8 It is yet to be fully established if the context-specific functions of autophagy have distinct requirements for select components of the autophagy pathway.
High levels of autophagy are induced in response to stress, such as nutrient deprivation, intracellular stress, high temperature, high culture density, hormones and growth factor deprivation.9, 10 The target of rapamycin (TOR) pathway is a central mediator in regulating the response to nutrients and growth signalling. TOR functions in two distinct complexes, with regulatory associated protein of TOR (Raptor) in TOR complex 1 (TORC1) or with rapamycin insensitive companion of TOR (Rictor) in TOR complex 2 (TORC2).11, 12, 13, 14, 15 Of these, TORC1 regulates autophagy; in nutrient-rich conditions, TORC1 activity inhibits the Atg1 complex preventing autophagy and cellular stress such as starvation leads to inactivation of TORC1 promoting a dramatic increase in autophagy. TORC2 can also negatively regulate autophagy via the FoxO3 complex in specific context.16
Most direct in vivo evidence for a role of autophagy in cell death has emerged from studies in Drosophila.5 Developmentally regulated removal of the Drosophila larval midgut can occur in the absence of canonical apoptosis pathway, whereas inhibiting autophagy delays the process.17, 18 Also, inhibition of autophagy leads to delayed degradation of larval salivary glands in Drosophila.19 Genetic studies have shown that many of the Atg genes known to be involved in starvation-induced autophagy in the Drosophila fat body are also involved in autophagy-dependent degradation of salivary glands and midgut.5, 20, 21 However, systematic studies to test whether starvation-induced autophagy and autophagy required for PCD require identical components have not been carried out, and there are some observations suggesting that there may be distinctions. For example, in Atg7-null mutants autophagy is perturbed but the larval–adult midgut transition proceeds normally.22 In addition, a novel Atg7- and Atg3-independent autophagy pathway is required for cell size reduction during midgut removal.23 Here we show that downregulation of TORC1 activity is required for induction of autophagy during midgut removal. Surprisingly, however, the requirement of part of the autophagy machinery during midgut degradation was found to be distinct to that which is required during autophagy induced by starvation. We report that Atg genes required for autophagy initiation, Atg8a and recycling are all essential for autophagy-dependent midgut removal, whereas other components of the elongation and nucleation steps are not essential.
Results
We have previously shown that knockdown of Atg1 and Atg18 or Atg2 and Atg18 mutants blocks autophagy and significantly delays larval midgut removal.17 However, it is unknown whether the signals that regulate this are similar to those regulating induction of autophagy in response to starvation and whether the canonical autophagy machinery components are required for this process. Here we dissect out the requirement of the components of the autophagy pathway by systematically knocking down Atg genes in the midgut and examining the effect on autophagy induction and midgut removal. The level of knockdown for each of the RNAi lines used was assessed by real-time qPCR and in all cases the lines that gave the best knockdown for the gene were selected for analyses (Supplementary Figure S1).
Autophagy induction: the Atg1 complex and its regulators
A key early step in the initiation of autophagy requires the activity of the Atg1 complex, comprising Atg1, Atg13, Atg17 (FIP200 in mammals) and Atg101.24, 25, 26 Similarly in Drosophila, Atg1 activity also requires Atg13, Atg17 and Atg101 in the complex.27, 28 Our previous work has shown that Atg1 is essential for autophagy and midgut removal.17 To determine the requirements of other subunits of the Atg1 complex in autophagy during midgut cell death, we examined the effects of knockdown of Atg13, Atg17 and Atg101. Similar to Atg1, depletion of Atg13, Atg17 or Atg101 resulted in significantly delayed midgut degradation (Figure 1). At the onset of PCD (0 h relative to puparium formation (RPF)) and during PCD when the gastric caeca have contracted in the control animals (+4 h RPF), Atg1, Atg13, Atg17 or Atg101 knockdown resulted in larger midguts (Figures 1a–f). The levels of pmCherry-Atg8a puncta were dramatically reduced upon knockdown of Atg1, Atg13, Atg17 or Atg101 at +2 h RPF compared with the control (Figures 1a–e and g). At later developmental stages, the dying midgut continues to condense and at +12 h RPF consists of a compact body. Compared with the control at +12 h RPF, pupae from Atg1, Atg13, Atg17 or Atg101 knockdown lines showed dramatically enlarged midgut consistent with delayed removal (Figures 1a–e). The delayed midgut degradation displayed in Atg17 knockdown animals is consistent with recent studies using Atg17 mutants.28 Thus our data indicate that all known components of the Atg1 complex are required for Drosophila midgut PCD.
Autophagosome nucleation: class III PI3K complex
During autophagosome initiation, the class III PI3K complex, consisting of Vps35, Vps15 and Atg6 (Beclin 1 in mammals), generates phosphatidylinositide-3-phosphate required for vesicle nucleation.29 Studies in yeast and mammalian cells have revealed that Atg14 controls phosphorylation of Atg6 and mediates the complex localisation to the preautophagosomal structure (PAS).30, 31 In Drosophila, Atg6, Vps34 (also known as Pi3K59F) and Vps15 (also known as ird1) form a complex required for starvation-induced autophagy in the fat body.32, 33 However, the function of Atg14 has not been characterised. To assess the role of the class III PI3K complex in midgut PCD, we examined the knockdown of Atg6, Atg14, Vps15 and Vps34. The knockdown of these genes showed midgut removal was affected to differing extents. Surprisingly, knockdown of Atg6 showed normal autophagy levels although the gastric caeca were larger compared with control (Figures 2a and b). This phenotype was also observed in an independent Atg6 knockdown line (Supplementary Figure S2). This is in contrast to Vps15 or Vsp34 knockdown, where the level of autophagy was reduced corresponding to enlargement of gastric caeca (Figures 2d–g). Quantitation of pmCherryAtg8a puncta showed the reduction of autophagy level in Vps15 and Vps34 knockdown was significant (Figure 2g). The knockdown of Atg14 showed only a modest increase in size with a correspondingly modest decrease in autophagy (Figures 2c, f and g). A similar result was seen with an Atg14-null mutant (not shown).
Autophagosome expansion: the Atg8 and Atg12 conjugation systems
Expansion of the phagophores into autophgagosomes requires two ubiquitin-like systems: the Atg8–lipid phosphatidylethanolamine (PE) conjugation system and the Atg12–Atg5 system (Figure 3a). Prior to lipidation, cleavage of Atg8 by the cysteine protease, Atg4, allows the E1-like enzyme Atg7 to interact with Atg8.34 Following this, Atg8 is then transferred to the E2-like enzyme Atg3 and finally to PE.35 Transfer of Atg8 from Atg3 to PE is in part catalysed by the final product of the second conjugation system, the Atg12–Atg5–Atg16 complex.36 Functioning in parallel to the Atg8–PE system, the Atg12–Atg5 conjugation system is similar to that of Atg8–PE system (Figure 3a). The E1- and E2-like enzymes are Atg7 and Atg10, respectively, and mediate the transfer of Atg12 to Atg5. Atg12 is finally conjugated to Atg5, forming an Atg12–Atg5 complex, which associates with Atg16 and assists the elongation of autophagosome.34, 36
To examine the contribution of the components of the Atg8a–PE conjugation system to autophagy in midgut PCD, we examined the knockdown of Atg3, Atg4a and Atg7. At the onset of PCD (0 h RPF) and at a later stage of midgut histolysis (+4 h RPF), depletion of Atg3, Atg4a or Atg7 had no significant effect on midgut removal (Figures 3b–e and i). Consistent with this, high levels of autophagy similar to the control at +2 h RPF were observed following Atg3, Atg4a or Atg7 knockdown (Figures 3b–e and j). This suggests that the Atg8–PE conjugation system, Atg3, Atg4a and Atg7, is not essential for autophagy in the midgut. This is in agreement with previous studies that indicate that Atg7 is not essential for midgut removal and that Atg3 and Atg4 are not required for cell size reduction in the midgut.23 Together this indicates that lipidation is not the rate-determining step or may not be required for the function of Atg8a in this context.
Interestingly, our analyses of the depletion of components of the Atg12–Atg5–Atg16 complex showed that knockdown of Atg5, Atg12 or Atg16 resulted in a moderate but significant delay in midgut removal (Figures 3f–i). At the onset (0 h RPF) and during (+4 h RPF) PCD, Atg5, Atg12 or Atg16 knockdown midguts were larger than the control (Figures 3b and f–i). This is consistent with the findings that clones of cells depleted for Atg5, Atg12 or Atg16 are larger than the neighbouring control cells.23 Surprisingly, however, examination of autophagy levels in the Atg5, Atg12 or Atg16 knockdown showed no significant difference in pmCherry-Atg8a puncta at +2 h RPF (Figures 3f–h and j). An additional marker of autophagy commonly used in Drosophila is lysotracker staining, and we observed overlap between lysotracker staining and pmCherry-Atg8a puncta in dying midguts (Supplementary Figure S3). Lysotracker staining of Atg5, Atg12 or Atg16 knockdown midguts as an alternative marker of autophagy did not show any obvious differences compared with the control, whereas they were dramatically different to Atg1 knockdown that blocks autophagy (Supplementary Figure S3).
Given that the knockdown of components of the Atg8a conjugation system had no dramatic effect on autophagy and midgut removal, we examined the contribution of Atg8a to midgut autophagy and PCD. There are two Atg8 paralogues annotated in the Drosophila genome, Atg8a and Atg8b. During the late larval stage, developmental autophagy is induced in the fat body with increased expression of both Atg8a and Atg8b.37 Similarly, Atg8a and Atg8b are transcriptionally induced during starvation in the fat body.8, 38 During midgut histolysis, the expression level of Atg8a is high compared with very low expression of Atg8b (data not shown). Consistent with the central role of Atg8a in autophagy, both Atg8a knockdown and mutant show dramatically delayed midgut removal and loss of autophagy (Figures 4a–c, e and f). At the onset of midgut removal (0 h RPF) and during histolysis (+4 h RPF), the Atg8a-depleted midguts were significantly larger than the controls (Figure 4a–c and e). The level of autophagy puncta was detected using an alternative marker, GFP-Atg5, which showed a block in autophagy in the Atg8a knockdown (Figures 4a, b and f). Similarly, autophagy puncta was significantly reduced in the Atg8a mutant midguts (Figures 4c and f). These data indicate that Atg8a has an essential role in midgut removal.
Selection of autophagic cargo can be determined by cargo receptors that interact with Atg8 on the isolation membrane. The multifunctional scaffold protein p62 (also known as SQSTM1 in mammals) binds ubiquitinated proteins and acts as a cargo receptor by binding Atg8 targeting ubiquitinated proteins for degradation by autophagy.39 The Drosophila p62 orthologue ref(2)P associates with ubiquitin-positive protein aggregates and accumulates when autophagy is blocked.40 Knockdown of ref(2)P in the midgut did not affect autophagy or midgut removal (Figures 4d–f), suggesting that it is not essential for autophagy-dependent PCD.
Preautophagosomal cycling complex: Atg9, Atg2 and Atg18
The transmembrane protein Atg9 is part of the core autophagy machinery and is required for autophagosome formation but is absent from the completed vesicle.29, 41, 42 There are two sources of Atg9, the intercellular pools of Atg9 and the Atg9 on the PAS.43 The recycling of Atg9 from the PAS to the intracellular pools is thought to be regulated by three protein complexes, including Atg1–Atg13, Atg2–Atg18 and the PI3K complex. All three complexes are required for effective bulk autophagy.25, 44, 45 The Atg1–Atg13 and the class III PI3K complexes, as described above, have a role in the early steps of autophagosome biogenesis as well, where they regulate the initial step of the double-membrane vesicle formation.46 Two functions for the Atg9 complex have been proposed; by reversibly binding to another Atg9 molecule it can both promote lipid transport to the PAS to facilitate nucleation, and also act to promote the assembly of an intact autophagosome membrane.43
Consistent with and in addition to our previous studies, the knockdown of Atg2, Atg9 and Atg18 resulted in delayed midgut removal (Figures 5a–e). The midguts at the onset (0 h RPF) and during histolysis (+4 h RPF) were significantly larger than the control and at a later time point (+12 h RPF) the midgut in the knockdown animals persisted while it was condensed in control (Figures 5a–e). Examination of autophagy puncta at +2 h RPF showed a significant block in autophagy induction in the Atg2, Atg9 and Atg18 knockdown (Figures 5a–d and f). These results suggest an essential role of Atg2, Atg9 and Atg18 in autophagy and midgut degradation.
Autophagy induction in response to starvation in the fat body
The Drosophila fat body undergoes rapid autophagy induction following starvation, and several Atg genes, including, Atg1, Atg3, Atg5, Atg7, Atg12 and Atg18, have been shown to function during starvation-induced autophagy in the fat body.8, 41 Given our findings that not all Atg genes are required for autophagy during midgut removal, we examined the effect of their knockdown in the fat body to determine whether the Atg genes are required during starvation-induced autophagy in this tissue. Knockdown of Atg genes in the fat body reveals that genes essential for autophagy during midgut removal are also required for starvation-induced autophagy in the fat body (Figure 6). Whereas Atg8a conjugation pathway genes (Atg3 and Atg7), Atg12 conjugation genes (Atg5, Atg12 and Atg16) and Atg6, which are not required for autophagy during midgut removal, are required for starvation-induced autophagy in the fat body (Figure 6). Taken together our data, directly comparing the core components of autophagy in midgut PCD and starvation-induced autophagy, provide strong in vivo evidence that the requirements of the autophagic machinery are distinct in specific tissues and contexts.
Downregulation of TORC1 is required for midgut removal
TORC1 is a crucial negative regulator of autophagy, and down regulation of TORC1 signalling promotes the activation of the autophagy initiation complex.47 Knockdown of Tor using two independent lines induced premature autophagy in the midgut and resulted in shortened gastric caeca at an early stage (−4 h RPF) (Figures 7a, b, e and f and Supplementary Figure S4). The severity of the phenotype (both the level of autophagy and gastric caeca size) correlated with the level of knockdown (Supplementary Figure S4). As rapamycin is a commonly used inhibitor of Tor, we fed larvae with food that contained rapamycin. Lysotracker staining shows that, following 2 h of rapamycin feeding to 96 h after egg deposition (AED) larvae, autophagy was induced in both fat body and midgut (Figure 7k). This further implies that Tor is negatively regulating the induction of autophagy, consistent with what has been shown during starvation-induced autophagy. Consistent with this, overexpression of Tor resulted in reduced autophagy with delayed midgut PCD (Supplementary Figure S4).
TOR can assemble into distinct complexes, TORC1 and TOCR2, with Raptor and Rictor, respectively. TORC1 is well established as a negative regulator of autophagy.42 Consistent with this role of TORC1, knockdown of raptor resulted in earlier autophagy induction and smaller midguts, whereas rictor knockdown had no effect (Figures 7a–f). The knockdown of Tor or raptor in the fat body was sufficient to induce autophagy under fed conditions that was not altered by starvation, whereas the knockdown of rictor showed robust induction of autophagy following starvation (Figures 7g–j). These results suggest that the negative control of autophagy by TORC1 during midgut removal is similar to the role of TORC1 during starvation-induced autophagy in the fat body.41
To examine the requirement of Atg1 downstream of Tor during midgut removal, we examined the combined knockdown of Atg1 and Tor. Knockdown of Tor showed shorter gastric caeca at an earlier but not later time point (Figure 8b) and autophagy was induced at an earlier stage (Figures 7b and 8b). Knockdown of Atg1 showed larger gastric caeca at both early and late times, accompanied by reduced autophagy (Figures 8c, e and f). Simultaneous knockdown of both Atg1 and Tor restored autophagy and gastric caeca contraction to levels similar to those seen in control (Figures 8d–f). Together this implies that in the absence of Tor, Atg1 is not essential or low levels are sufficient for induction of autophagy and suggests that Tor may act in multiple pathways to inhibit autophagy.
Discussion
Our work provides a detailed analysis of the requirements for canonical autophagy components in regulation of Drosophila larval midgut PCD. We show that there is a distinct requirement for specific components for midgut autophagy and PCD that differs from autophagy induced in the fat body in response to starvation. We also demonstrate that, despite requiring specific Atg genes, the upstream regulation of autophagy by TORC1 in the midgut appears to be similar to that regulating starvation-induced autophagy. Surprisingly though, our data suggest that, in the absence of Tor, Atg1 is not essential for autophagy induction. This implies that an alternative mechanism regulating autophagy induction may exist, and it will be important to dissect out this pathway in future studies.
We have shown that the components of the initiation complex are essential for induction of autophagy and midgut degradation. Depletion of Atg1, Atg13, Atg17 or Atg101 blocked autophagy induction and dramatically delayed midgut removal. These finding are consistent with the conserved role of this complex in autophagy induction in other systems.
The class III PI3K complex, consisting of Atg6, Vps15 and Vps34, contributes to autophagsome formation, by recruiting phosphatidylinositol 3-phosphate and other proteins (such as Atg18).35, 46 Consistent with our result in the fat body with Atg6 knockdown, the loss-of-function Atg6 mutants shows disruption of autophagy in the fat body.33, 41 Surprisingly, knockdown of Atg6 did not reduce autophagy in midgut yet delayed midgut histolysis. This could suggest that Atg6 has an alternative role in midgut degradation other than autophagy. Atg6 is required for starvation-induced autophagy in the fat body and also has roles in vesicle trafficking and haematopoiesis.33 This multifunction of the PI3K complex may explain the different requirements for Vps15 and Vps34 in midgut removal and autophagy.48 Other autophagy-independent processes of Vps15 and Vps34 include regulation of protein secretion and other vesicular trafficking processes, including protein sorting.49
Our data suggest that there are different requirements for the two conjugation pathways during midgut PCD. Although Atg8a has an essential role in midgut removal, the components required for lipidation are not essential. An alternative conjugation pathway has been proposed, and that the function of Atg8a is independent of its lipidation thus Atg3 and Atg7 function is not required.23 However, the molecular mechanism of this pathway remains to be established. The E3-like complex consisting of Atg5 and Atg12 promotes the transfer of Atg8 from Atg3 to PE and facilitates autophagosome expansion. The knockdown of Atg5, Atg12 or Atg16 resulted in a moderate delay in midgut degradation, with no significant effect on autophagy. This is in contrast to the essential role of these components in starvation-induced autophagy and suggests that an alternative conjugation pathway may compensate.
The molecular functions of Atg2 and Atg9 have not been characterised in Drosophila. The yeast orthologues function in lipid and protein recycling and assembly of autophagic membrane.43 Additionally, the initiation of autophagy requires direct phosphorylation of Atg9 by Atg1, which is required for recruitment of Atg8 and Atg18 to the autophagosome formation site and expansion of the isolation membrane.50 Our data show that knockdown of Atg2, Atg9 or Atg18 result in a similar delay in midgut degradation and disruption of autophagy. Combined with earlier observations,17, 19 our data here suggest that the Atg2, Atg9 and Atg18 complex is essential for the initiation of autophagy in the midgut, rather than only working as a recycling mechanism.
Although the components of the autophagy pathway required for midgut degradation differ to that required for starvation-induced autophagy in the fat body, we demonstrate that in both cases TORC1 negatively regulates the initiation of autophagy. The level of autophagy and extent of midgut degradation were found to be directly correlated with TORC1 function. Consistent with this, raptor depletion resulted in premature autophagy similar to Tor ablation, suggesting that raptor and Tor function in TORC1 and negatively regulates autophagy in this cell death context.
Our study shows that distinct components of the core autophagy machinery are required during midgut PCD (Supplementary Figure S5). Furthermore, the findings here reveal that while some Atg proteins are not required for autophagosome formation they are required for the cell size reduction that occurs during midgut histolysis. This intriguing finding suggests that alternative pathways of autophagy function in specific tissues with the potential for autophagy-independent roles of Atg proteins. It is likely that the different forms of autophagy may require distinct regulatory components and this may be based on specific initiating signals. Autophagy can be regulated by numerous pathways, and while most centre around TOR activity, TOR-independent regulation can also occur. It will be important to determine the nature of the upstream signalling during autophagy-dependent midgut PCD as well as determining the non-autophagic function of Atg genes. It is possible that distinct forms of autophagy are required depending on the rate of autophagy required, or if it needs to be maintained for an extended time and the cellular outcome (whether the engulfed material is being recycled or degraded). Our data shows that the cargo receptor p62 is not essential for autophagy. This suggests that the nature of the cytoplasmic targets may have a role, whether non-selective bulk degradation of cytoplasmic targets is required or whether specific cargo are selected. All these factors may contribute to context-specific requirements for distinct autophagy machinery.
Overall, as summarised in Supplementary Figure S5, our study suggests that somewhat different mechanisms execute autophagy in dying midguts and fat body under starvation. Further studies will be required to fully delineate the regulation and mechanisms that mediate autophagy-dependent cell death in the midgut and to understand what makes autophagy required for PCD distinct from starvation-induced autophagy.
Materials and Methods
Fly stocks
We used midgut driver NP1-GAL4, obtained from the Drosophila genetic resource center (Kyoto, Japan) crossed to UAS-LacZ RNAi (obtained from Kennerdell and Carthew51) or w1118 (Bloomington Stock Center, Bloomington, IN, USA) as control. To drive expression in fat body, CG-GAL4 was used. To monitor autophagy induction, we used pmCherry-Atg8a52 and UAS-eGFP-Atg5 (Bloomington Stock Center). The lines to knockdown Atg genes, UAS-RNAi, were obtained from the Bloomington Stock Center or Vienna Drosophila RNAi Center (Vienna, Austria) as listed in Table 1. All flies were maintained and crossed\s performed at 25 °C on cornmeal, molasses and yeast medium. Larvae were grown on food containing 0.05% bromophenol blue and staged by collection of wandering clear gut larvae onto damp Whatmann paper in a petri dish as previously described.17, 52
Detection and quantitation of autophagy in the midgut
Because of a lack of suitable reagents for monitoring autophagy flux, Atg8a puncta is commonly used for detecting autophagy in vivo in Drosophila.53 Autophagy was assessed by pmCherryAtg8a puncta formation.52 Additionally, eGFP-Atg5 puncta was also used to measure autophagy.17 In the case of Atg8a knockdown, eGFP-Atg5 was used as the autophagy level indicator to avoid a false result. Midguts from pupae staged +2 h RPF were dissected in PBS, stained with Hoechst 33342 (Sigma-Aldrich, St. Louis, MO, USA) and imaged immediately without fixation using Zeiss LSM-700 (Jena, Germany) confocal microscope. From at least 10 confocal images for each genotype, ImageJ (Bethesda, MD, USA) was used to count puncta with a size >2 pixels for all experiments unless otherwise stated in the selected channel and represented as the average puncta per cell. Images of Atg8aKG07569 Figure 4c and the control group plotted in Figure 4f with Atg8aKG07569 were taken using a Bio-Rad confocal microscope (Hercules, CA, USA) as previously described.17 Due to the background on Bio-Rad confocal images, particle >4 pixels were counted in ImageJ.
Midgut morphology and quantitation
Animals were staged −4 h, 0 h or +4 h RPF, and midguts were dissected in PBS, fixed in 4% formaldehyde/PBS and examined using a stereozoom microscope (Olympus, Tokyo, Japan). Measurements of gastric caeca size were done using Photoshop (Adobe, San Jose, CA, USA) magnetic lasso tool. The histogram function was used to determine pixels included in the area as previously described, including at least 10 midguts in each sample.52
Histology
Whole pupae staged to +12 h RPF were fixed in FAAG (85% ethanol, 4% formaldehyde, 5% acetic acid and 1% glutaraldehyde), paraffin embedded before sectioning and then haematoxylin and eosin stained as previously described.17 For each genotype, a minimum of 10 pupae were examined using a stereozoom microscope.
Autophagy in fat body
Larvae were aged in normal food 96 h after egg deposition (AED) and transferred to petri-dish for 2h starvation. The fat body was dissected in PBS with 1μM LysoTracker Green DND 26 (Invitrogen, Carlsbad, CA, USA) and Hoechst 33342.
Imaging
Confocal images were obtained using a confocal microscope with Argon ion 488 nm (14 mw) and Green HeNe 543 nm (1.5 mw) lasers and a Zeiss inverted microscope with a 40 × UPLAPO (NA=1.2 water) objective. The dual-labelled samples were imaged with two separate channels (PMT tubes) in a sequential setting. Green fluorescence was excited with an Ar 488 nm laser line, and the emission was viewed through a HQ515/30 nm narrow band barrier filter in PMT1. Red fluorescence was excited with a HeNe 543 nm laser line, and the emission was viewed through a long pass barrier filter (E570LP) in PMT2. Automatically, all signals from PMTs 1 and 2 were merged. Images were captured using the Zen (Jena, Germany) software and compiled using Photoshop CS5 (Adobe). In all experiments, samples were immediately imaged with a laser-scanning confocal microscope (Zeiss LSM-700) unless otherwise stated.
Rapamycin feeding
Larvae were aged in normal food 96 h after egg deposition (AED) and transferred to food containing the indicated concentration of rapamycin for 2 h (original stock 1 mM dissolved in DMSO). 0.05% Bromophenol blue was also added to the food to ensure uptake of treated food that would appear blue in the gut. Food containing same volume of DMSO was used as control treatment. Lysotracker is also widely used as an autophagy-level indicator especially in Drosophila fat body.41 We have also shown that lysotracker and pmCherryAtg8a co-localize in dying midgut (Supplementary Figure S3). Thus in the rapamycin experiments lysotracker was used as an indicator. The fat body and midguts were dissected in PBS with 1 μM LysoTracker Red DND 99 and Hoechst 33342.
Quantitative real-time PCR
Quantitation of the level of RNAi knockdown of various lines was done by RT-qPCR. Total RNA was isolated from Drosophila midguts using TRIzol reagent (Invitrogen) and cDNA was synthesized from 1 μg of total RNA using High Capacity cDNA Reverse Transcription Kit (Applied Biosciences, Foster City, CA, USA) and oligo dT primer. Real-Time qPCR was performed on a Rotor-Gene 6000 (Corbett Research, Mortlake, NSW, Australia) using RT2 Real-Time SYBR Green/ROX PCR MasterMix (Qiagen, Valencia, CA, USA) as per the manufacturer’s instructions. Reactions were performed in triplicate, and the mRNA expression levels were normalized against the internal control gene rp49 using the ΔΔCT method. Primer sets used were:
rp49 F CCAGTCGGATCGATATGCTAA; R ACGTTGTGCACCAGGAACTT
Atg1 F ACGGCGGACAAGATTCTCTA; R GCTGCTGCAATATGCTCAAA
Atg2 F AAGTGGAAGCAGGAGATCCA; R AATCACACCCATGTCGGAAT
Atg3 F GCAATGTTCCCTGCTATCGT; R TGTCGCTATCTGGAGTGTGC
Atg4 F CTTCTCCCTGCACTCGTTTC; R CTCGGTTTGATGGGATGACT
Atg6 F TTCCAGAAGGAGGTCGAGAA; R TCCCCATTTCAGGTTGGTTA
Atg7 F GGAATGCTGTGCAACTACGA; R GGAGATTCCCGTCAAATCCT
Atg8a F CATCGGTGATTTGGACAAGA; R TGCCGTAAACATTCTCATCG
Atg8b F TCAGTACCAGCAGAAGCACG; R AAGGCAAGTAGCCAGACGAC
Atg9 F CGGAGCTGCACAAGAAGAAG; R CTTGTCGAAGTGCTCCTGGT
Atg12 F CAATGTGCCCATCATCAAAA; R CACGCCTGATTCTTGCAGTA
Atg13 F ACAGCCACATATCGTCACCA; R GGTCGAGGAAATCGATGAGA
Atg14 F GTCAGAGGACGAAAACTCTC; R ATGGTAGACTGCTGGTTGG
Atg16 F ATGCATCGATGTGCCGTGA; R CTTGCTTGCAGGATTCGTGG
Atg17 F CGTCTCCCTTCTTTGAGTCG; R CCAAACGTGGAGCCATTAGT
Atg18 F AGGTGACCGACGTGTTTAGC; R ACGGTGGGAATGGAATACAC
Atg101 F CGAGAACGAGGATGAGAGGC; R ACGTCCGGAAAGGATGTGTC
Vsp15/ird1 F GAGCCGTAGTCCTTGGAGTG; R TGTCACCAGCTTGTCGTAGC
Vsp34/PI3K59F F AGCCAATCTCTGCACATTCC; R GCCTCTCAGGTTGAAGTTGC
ref(2)P F AAACCACCACCGAAACAGAG; R CCTCCTCATTCATGTGCTGA
rictor F GCAGCCAATGAGTTACAGCA; R GAGCGTAGCTGGACATAGCC
raptor F TCGGTTCTTTGAGGGAGCTA; R CGGCTGTCTCTCGTACATCA and
Tor F CGGTTATCCCGCTCAGTACC; R GGTGATCATAGTCTGGCGCA
Statistical analysis of data
Student’s t-test was used for all statistical analysis unless otherwise stated. Data are expressed as mean±S.D. or ±S.E.M., as appropriate. P<0.05 was considered significant.
Abbreviations
- Atg:
-
autophagy-related
- PCD:
-
programmed cell death
- PI3K:
-
phosphatidylinositol 3-kinase
- RPF:
-
relative to puparium formation
- TOR:
-
target of rapamycin
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Acknowledgements
This work was supported by the National Health and Medical Research Council of Australia Project Grant (1041807) and a Senior Principal Research Fellowship (1002863) to SK. TX was supported by a University of South Australia President’s Scholarship and SN by an Australian Postgraduate Award. We thank Sonia Dayan, May Aung-Htut and other members of our laboratory for help and advice, the Australian Drosophila Research Support Facility, Vienna Drosophila RNAi Centre and Bloomington Drosophila Stock Center for Drosophila stocks and Amanda Rogers for tissue processing.
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Xu, T., Nicolson, S., Denton, D. et al. Distinct requirements of Autophagy-related genes in programmed cell death. Cell Death Differ 22, 1792–1802 (2015). https://doi.org/10.1038/cdd.2015.28
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DOI: https://doi.org/10.1038/cdd.2015.28
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