Abstract
Ferrous iron (Fe2+) toxicity is a major disorder in rice prod uction on acid, flooded soils. Rice ( Oryza sativa L.) genotypes differ widely in tolerance to Fe2+ toxicity, which makes it possible to bre ed more tolerant rice varieties. Tissue tolerance to higher iron concentrations in plants has been considered to be important to Fe2+ tolerance in ri ce. Segregation for leaf bronzing and growth reduction due to Fe2+ to xicity was observed in a doubled haploid (DH) population with 135 lines derived from a Fe2+ tolerant japonica variety, Azucena, and a sensitive indic a variety, IR64 in a solution culture with Fe2+ stress condition at a Fe2+concentration of 250 mg L-1 at pH 4.5. To better understand the mechanism of tissue tolerance, Leaf Bronzing Index (LBI), total iron concentration in shoot tissue and the enzymes of ascorbate peroxidase (AP), dehydroascorbate reductase (DR) and glutathione reductase (GR), and concentrations of ascorbate (AS) and dehydroascorbate (DHA), which are involved in the ascorbate-specific H2O2-scavenging system, were determined for the population under Fe2+ stress. A non-normal distribution of LBI was found. About 38 lines showed no bronzing, while the lines with non-zero LBI values ranged from 0.05 to 0.85 and showed a normal distribution. The other parameters measured showed normal distribution. The total iron concentrations in the 38 tolerant lines ranged from 1.76 mg Fe g-1 to 4.12 mg Fe g-1 and was in a similar range as in the non-tolerant genotype (2.04 – 4.55 mg Fe g-1). No significant differences in the activities of the enzymes were found between the parents under normal culture, but remarkably higher Fe2+ induced enzyme activities were observed in the tolerant parent. AS was similar between the parents under both normal and Fe2+ stress, but its concentration was sharply decreased under Fe2+ stress. DHA was much lower in the tolerant parent than in the sensitive parent under Fe2+ stress. Single locus analysis and interval mapping analysis based on 175 molecular markers revealed that the interval flanked by RG345 and RZ19 on chromosome one was an important location of gene(s) for Fe2+ tolerance. The ascorbate-specific system for scavenging Fe2+-mediated oxygen free radicals may be an important mechanism for tissue Fe2+ tolerance. A gene locus with relative small effect on root ability to exclude Fe2+ was also detected.
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References
Asada K 1992 Ascorbate peroxidase — a hydrogen peroxidase-scavenging enzyme in plants. Physiol. Plant. 85, 235–241.
Asada K and Badger M R 1984 Photoreduction of 18O2 and H2 18O2 with a concomitant evolution of 18O2 in intact spinach chloroplasts: Evidence for scavenging of hydrogen peroxide by peroxidase. Plant Cell Physiol. 25, 1169–1179.
Asada K and Takahashi M 1987 Production and scavenging of active oxygen in photosynthesis. In Photoinhibition. Eds. D J Kyle, C B Osmond and C J Arntzen. pp 227–287. Elsevier Science Publishers, Amsterdam.
Briat J F 1996 Roles of ferritin in plants. J. Plant Nutr. 19(8,9), 1331–1342.
Bode K, Doring O, Luthje S, Neue H U and Bottger M 1995 The role of active oxygen in iron tolerance of rice (Oryza sativa L.). Protoplasma 184, 249–255.
Bradford M M 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.
Causse M, Fulton T M, Cho Y G, Ahn S N, Chunwongse J, Wu K, Xiao J, Yu Z H, Ronald P C, Harrington S B, Second G A, McCouch S R and Tanksley S D 1994 Saturated molecular map of the rice genome based on an inter-specific backcross population. Genetics 138, 1251–1274.
Conklin P L and Last R L 1995 Differential accumulation of antioxidant mRNA in Arabidopsis thaniana exposed to ozone. Plant Physiol. 109, 203–209.
Ezaki B, Tsugita S and Matsumoyo H 1996 Express of a moderately anionic peroxidase is induced by aluminum treatment in tobacco cells: Possible involvement of peroxidase isozymes in aluminum ion stress. Physiol. Plant. 96, 21–28.
Fageria N K and Rabelo N A 1987 Tolerance of rice cultivars to iron toxicity. J. Plant Nutr. 10, 653–661.
Foyer C H and Halliwell B 1976 The presence of glutathione and glutathione reductase in chloroplasts: Proposed role in ascorbic acid metabolism. Planta 133, 21–25.
Guiderdoni E, Galinato E, Luistro J and Vergara G 1992 Anther culture of tropical japonica x indica hybrids of rice (Oryza sativa L.). Euphytica 62, 219–224.
Gunawarkena I, Virmani S S and Sumo F J 1982 Breeding rice for tolerance to iron toxicity. Oryza 19, 5–12.
Halliwell B and Gutteridge J M C 1984 Lipid peroxidation, oxygen radicals, cell damage and antioxidant therapy. Lancet 1, 1396.
Halliwell B and Gutteridge J M C 1989 Free radicals in biology and medicine. 2nd edn. Oxford University Press, Oxford.
Harrison P M, Hoy T G and Macara I G 1974 Ferritin iron uptake and release. Biochem. J. 143, 445–451.
Hossain M A and Asada K 1984 Purification of dehydroascorbate reductase from spinach and its characterization as a thiol enzyme. Plant Cell Physiol. 25, 85–92.
Hossain M A and Asada K 1985 Monodehydroascorbate reductase from cucumber is a flavin adenine dinucleotide enzyme. J. Biol. Chem. 260, 12920–12926.
Hossain M A, Nakano Y and Asada K 1984 Monodehydroascorbate reductase in spinach chloroplast and its participation in regeneration of ascorbate for scavenging hydrogen peroxide. Plant Cell Physiol. 25, 385–395.
Hu B, Zhu J, Wu Y, Luo A and Wu P 1997 Effect of POD on tolerance to ferrous iron toxicity in rice (English abstract). J. Zhejiang Agricultural University 23(5), 557–560.
Huang N, Parco A, Mew T, Magpantay G, McCouch S, Guiderdoni E, Xu J, Subudhi P, Angeles R and Khush G S 1997 RFLP mapping of isozymes, RAPD and QTLs for grain shape, brown plant hopper resistance in a doubled haploid rice population. Mol. Breed. 3, 105–113.
International Rice Research Institute (IRRI) 1965 Annual report 1964. Los Banos, Philippines. 335 p.
International Rice Research Institute (IRRI) 1996 Standard evaluation system for rice. 4th edn. Manila, Philippines, 35 p.
Ishizuka Y 1961 Onorganic nutrition of rice plant. Part 6. Effect of iron, manganese and copper level in culture solution on yields and chemical composition of the plant. J. Sci. Soil Manure, Jpn. 32, 97–100.
Jayawardena S D G, Watabe T and Tanaka K 1977 Relation between root oxidizing power and resistance to iron toxicity in rice. Report of Society of Crop Science and Breeding in Kinki, Japan, 22, 38.
Kampfenkel K, Marc V M and Dirk 1995 Effects of iron excess on Nicotiana plumbaginifolia plant. Plant Physiol. 107, 725.
Kurata N, Nagamura Y, Yamamoto K, Harushima Y, Sue N, Wu J, Antonio B A, Shomura A, Shimizu T, Lin S Y, Inoue T, Fukuhara A, Shimano T, Kuboki Y, Toyama T, Miyamoto Y, Kirihara T, Hayasaka K, Miyao A, Monna L, Zhong H S, Tamura Y, Wang Z X, Momma T, Umehara Y, Yano M, Sasaki T and Minobe Y 1994 A 300 kilobase interval genetic map of rice including 883 expressed sequences. Nature Genet. 8, 365–372.
Lander E S 1993 Mapmaker/Exp 3.0 and Mapmaker/QTL 1.1, Tutorial and Reference Manual. Whitehead Institute, 9 Cambridge Center, Cambridge, MA 02142.
Leshem Y Y, Halevy A and Frenkel A H 1986 Free radicals and senescence. In Processes and Control of Senescence. Elsevier, Amsterdam. pp 101.
Luo A, Jing G, Wu P, Ni J, Jiang S and Zhang Y 1997 Rice genotypic differences in nutrient status under excessive Fe2+ conditions. J. Plant Nutr. 20(10). (In press).
Nakano Y and Asada K 1981 Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 22, 867–880.
Okuda A and Takahashi E 1965. The role of silicon. In International Rice Research Insititute. The mineral nutrition of the rice plant. Johns Hopkins Press, Baltimore, Maryland. 123–146.
Ota Y 1968 Studies on the occurrence of the physiological disease called ‘bronzing’. Bull. Natl. Inst. Agric. Sci. (Japan) D 18, 31–104.
Peng X and Yamauchi M 1993 Ethylene production in rice bronzing leaves induced by ferrous iron. Plant Soil 149, 227–234.
Ponnamperuma F N, Bradfield R and Peech M 1955 Physiological desease of rice atributable to iron toxicity. Nature 175, 265.
Robert A, Isaac W and Wolliam C 1975 Collaborative study of wet and dry ashing techniques for the elemental analysis of plant tissue by atomic absorption spectrophotometry. J. AOAC 58, 436–440.
Schonener S and Krause G H 1990 Protective system against active oxygen species in spinach response to cold in excess light. 180, 383–394.
Smirnoff N and Colombe S V 1988 Drought influence the activity of enzymes of the chloroplast hydrogen peroxide scavenging system. J. Exp. Bot. 39, 1097–1108.
Tanaka A and Yoshida S 1970 Nutritional disorders of the rice plant in Asia. IRRI Tech. Bull. 10, 51.
Tanaka A, Loe R and Navasero S A 1966 Some mechanisms involved in the development of iron toxicity symptoms in the rice plant. Soil Sci. Plant Nutr. 12, 158–164.
Wu P, Luo A, Zhu J, Yang J, Huang N and Senadhira D 1997 Molecular marker linked to genes underlying seedling tolerance for ferrous iron toxicity. Plant Soil 196, 317–320.
Yamanouchi M and Yoshida S 1982 The difference of leaf tissues to tolerance for iron toxicity among rice varieties. Plant Sci. Plant Nutr. 28, 983.
Yamauchi M and Peng X 1995 Iron toxicity and stress-induced ethylene production in rice leaves. Plant Soil 173, 21.
Yoshida S, Forno D A, Cock J H and Gomez K A 1976 Laboratory manual for physiological studies of rice. 3rd edn. Int. Rice Res. Inst., Manila, Philippines. pp 61–64.
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Wu, P., Hu, B., Liao, C. et al. Characterization of tissue tolerance to iron by molecular markers in different lines of rice. Plant and Soil 203, 217–226 (1998). https://doi.org/10.1023/A:1004321218387
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DOI: https://doi.org/10.1023/A:1004321218387