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Effects of Selol 5% supplementation on tissue antioxidant enzyme levels and peroxidation marker in healthy mice

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Abstract

Background

Selenium (Se) is an essential micronutrient for animals and humans used in the prevention or treatment of cancer. Selol is a mixture of selenitetriglycerides, containing Se(IV). It does not exhibit mutagenic activity and is less toxic than inorganic sodium selenite containing Se(IV). The antioxidant properties of the Selol were demonstrated using the blood of healthy animals. The aim of the study was to evaluate Selol as a Se supplement by determining the effect of its administration on the Se level and the antioxidant status in the tissues.

Methods

We examined the effect of long-term (28-day) Selol 5% supplementation on the activity of antioxidant enzymes, including the main selenoenzymes in healthy mice organs, such as liver, brain, lungs, and testis. Enzyme activities of the tissue homogenates and the concentration of malondialdehyde (MDA) as a biomarker of oxidative stress were measured using spectrophotometric methods. The selenium concentrations in the tissues were determined by inductively coupled plasma mass spectrometer (ICP-MS) as well.

Results

A significant increase in glutathione peroxidase, thioredoxin reductase, and glutathione S-transferase activity as well as the MDA concentration was observed in most of the studied tissues during the Selol 5% supplementation.

Conclusions

Long-term supplementation with the new Se(IV) compound — Selol 5% significantly affects the activity of antioxidant enzymes and the redox state in healthy mice organs. In the healthy population Selol 5% seems to be a promising new antioxidant compound.

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Authors and Affiliations

  1. Department of Bioanalysis and Drugs Analysis, Faculty of Pharmacy, Medical University of Warsaw, Warszawa, Poland

    Małgorzata Sochacka, Joanna Giebułtowicz, Piotr Suchocki & Piotr Wroczyński

  2. Department of Pharmacology, National Medicines Institute, Warszawa, Poland

    Małgorzata Remiszewska

Authors
  1. Małgorzata Sochacka
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  2. Joanna Giebułtowicz
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  3. Małgorzata Remiszewska
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  4. Piotr Suchocki
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  5. Piotr Wroczyński
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Correspondence to Małgorzata Sochacka.

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Sochacka, M., Giebułtowicz, J., Remiszewska, M. et al. Effects of Selol 5% supplementation on tissue antioxidant enzyme levels and peroxidation marker in healthy mice. Pharmacol. Rep 70, 1073–1078 (2018). https://doi.org/10.1016/j.pharep.2018.06.003

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  • DOI: https://doi.org/10.1016/j.pharep.2018.06.003

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