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Anti-inflammatory and ameliorative effects of gallic acid on fluoxetine-induced oxidative stress and liver damage in rats

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Abstract

Background

Fluoxetine-induced liver damage is a cause of chronic liver disease. In the present study the hepatoprotective effects of gallic acid against fluoxetine-induced liver damage were examined.

Methods

Forty-eight male rats were divided into six groups as follow: group 1, the control group; group 2, rats receiving fluoxetine (24 mg/kg bw daily, po) without treatment; group 3, rats receiving 24 mg/kg bw fluoxetine, treated with 50 mg/kg bw silymarin and groups 4, 5, and 6 in which gallic acid (50, 100, and 200 mg/kg bw, po, respectively) was prescribed after the consumption of fluoxetine. The histopathological changes of hepatic tissues were checked out.

Results

Fluoxetine caused a significant increase in the levels of serum glutamate oxaloacetate transaminase (GOT), serum glutamate pyruvate transaminase (GPT), lipid profiles, urea, fasting blood sugar (FBS), creatinine (Cr), protein carbonyl (PC) content, malondialdehyde (MDA), and liver TNF-α as an inflammatory element. Also, the obtained results of group 2 revealed a significant decline in ferric reducing ability of plasma (FRAP), liver catalase (CAT), superoxide dismutase (SOD), and vitamin C levels. The treatment with gallic acid showed significant ameliorations in abnormalities of fluoxetine-induced liver injury as represented by the improvement of hepatic CAT, SOD activities, vitamin C levels, serum biochemical parameters, and histopathological changes, in addition to the recovery of antioxidant defense system status.

Conclusions

Gallic acid has inhibitory effects on fluoxetine-induced liver damage. The effect of gallic acid is derived from free radical scavenging properties and the anti-inflammatory effect related to TNF-α.

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Authors and Affiliations

  1. Student Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Omid Karimi-Khouzani

  2. Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Esfandiar Heidarian

  3. Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Sayed Asadollah Amini

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  1. Omid Karimi-Khouzani
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  2. Esfandiar Heidarian
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  3. Sayed Asadollah Amini
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Correspondence to Esfandiar Heidarian.

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Karimi-Khouzani, O., Heidarian, E. & Amini, S.A. Anti-inflammatory and ameliorative effects of gallic acid on fluoxetine-induced oxidative stress and liver damage in rats. Pharmacol. Rep 69, 830–835 (2017). https://doi.org/10.1016/j.pharep.2017.03.011

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