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The influence of electrical stimulation on dorsal raphe nucleus with different current intensities on morphine-induced conditioned place preference in male rats

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Abstract

Introduction

The dorsal raphe nucleus (DRN) influences a wide range of behavioral and physiological processes. The purpose of the present study was to test the effects of electrical stimulation of the DRN with different current intensities on morphine-induced conditioned place preference (CPP).

Materials and methods

Male Wistar rats were divided for experimental groups (n = 7). Stimulating electrodes were stereotaxically implanted into the DRN in anesthetized rats. We investigated the influences of electrical stimulation of the DRN with different current intensities with ineffective and effective dose of morphine (0.5 and 2.5 mg/kg, respectively) on morphine-induced CPP.

Result

Subcutaneous administration of morphine 2.5 mg/kg produced significant CPP in comparison with saline group. The stimulation of the DRN with different current intensities (10, 25, 50 and 100 μA) in combination with an effective dose of morphine did not show significant differences on acquisition phases, whereas there were significant decreases on expression phases versus to the morphine group on CPP only in current intensity 100 μA. The stimulation of the DRN with different current intensities in combination with an ineffective dose of morphine showed significant increases in current intensities (50 and 100 μA) on acquisition phases of CPP, but did not show significant differences on expression phases versus to the morphine group on CPP.

Conclusion

It is possible that electrical stimulation of the DRN with changes in concentration of serotonin or involving other transmitters such as glutamate and GABA would be involved with these changes of CPP.

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Authors and Affiliations

  1. Department of Physiology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

    Gholam Reza Ghavipanjeh, Ali Asghar Pourshanazari & Hojjatllah Alaei

  2. Department of Physiology, Faculty of Sciences, Payam Noor Isfahan University, Isfahan, Iran

    Sara Karimi

Authors
  1. Gholam Reza Ghavipanjeh
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  2. Ali Asghar Pourshanazari
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  3. Hojjatllah Alaei
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  4. Sara Karimi
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Correspondence to Ali Asghar Pourshanazari.

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Ghavipanjeh, G.R., Pourshanazari, A.A., Alaei, H. et al. The influence of electrical stimulation on dorsal raphe nucleus with different current intensities on morphine-induced conditioned place preference in male rats. Pharmacol. Rep 67, 832–836 (2015). https://doi.org/10.1016/j.pharep.2015.01.006

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  • DOI: https://doi.org/10.1016/j.pharep.2015.01.006

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