Abstract
Eumops is the most speciose and morphologically diverse genus of Neotropical molossid bats presently encompassing 16 species. Here were described a new species in the genus based on three specimens from southeastern Bolivia and Brazil. A combined phylogenetic analysis using Cytochrome b gene sequences and morphological data revealed that the specimens form a sister-group of Eumops perotis + Eumops trumbulli and are 7.9% diverged from E. perotis at Cytochrome b. Three morphological autapomorphies, the shape of rostrum and the position and size of first upper premolar, distinguish new species from E. perotis and E. trumbulli. The combination of morphological and molecular characters justifies the description of a new species.
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Akaike, H., 1974. A new look at the statistical model identification, IEEE Trans. Autom. Control 19, 716–723.
Ammerman, L.K., Lee, D.N., Tipps, T.M., 2012. First molecular phylogenetic insights into the evolution of tree-tailed bats in the subfamily Molossinae (Molossidae, Chiroptera), J. Mammal. 93, 12–28.
Baker, R.J., Bradley, R.D., 2006. Speciation in mammals and the genetic species concept, J. Mammal. 87, 643–662.
Baker, R.J., Porter, C.A., Patton, J.C., Van Den Bussche, R., 2000. Systematics of bats of the family Phyllostomidae based on RAG2 DNA sequences, Occas. Pap. Texas Tech Univ. 202, 1–16.
Baker, R.J., McDonough, M.M., Swier, V.J., Larsen, PA, Carrera, J.P., Ammerman, L.K., 2009. New species of bonneted bat, genus Eumops (Chiroptera: Molossidae) from the lowlands of western Ecuador and Peru, ActaChiropterol. 11, 1–13.
Bartlett, S.N., McDonough, M.M., Ammerman, L.K., 2013. Molecular systematics of bonneted bats (Molossidae: Eumops) based on mitochondrial and nuclear DNA sequences, J. Mammal. 94, 867–880.
Bradley, R.D., Baker, R.J., 2001. A test of the genetic species concept: cytochrome-b sequences and mammals, J. Mammal. 82, 960–973.
Brown, R.E., 1967. Baculaof some New World molossid bats, Mammalia 31, 645–667.
Brown, R.W., 1956. Composition of Scientific Words. Smithsonian Institution Press, Washington D.C., USA.
Brown, M.T., Wicker, L.R., 2000. Discriminant analysis. In: Tinsley, H.E.A., Brown, S.D. (Eds.), Handbook of Applied Multivariate Statistics and Mathematical Modeling. Academic Press, San Diego, USA, pp. 209–235.
Czaplewski, N.J., 1997. Chiroptera. In: Kay, R.F., Madden, R.H., Cifelli, R.L., Flynn, J.J. (Eds.), Vertebrate Paleontology in the Tropics: The Miocene fauna of La Venta, Colombia. Smithsonian Institution Press, Washington D.C., USA, pp. 410–431.
Dolan, P.G., Honneycutt, R.L., 1978. Systematic and evolutionary implications of genic variation in the mastiff bat, Eumops (Chiroptera: Molossidae). Bat Res. News 19, 72.
Edgar, R.C., 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput, Nucleic Acids Res. 32, 1792–1797.
Eger, J.L., 1977. Systematics of the genus Eumops (Chiroptera Molossidae), Life Sci. Contrib. R. Ont. Mus. 110, 1–69.
Eger, J.L., 2008. Family Molossidae. In: Gardner, A.L. (Ed.), Mammals of South America Vol 1: Marsupials, Xenarthrans, Shrews and Bats., 2nd. ed. Smithsonian Institution Press, Washington D.C., USA, pp. 399–440.
Farias, I.P., Orti, G., Sampaio, I., Schneider, H., Meyer, A., 2001. The Cytochrome b gene as a phylogenetic marker: the limits of resolution for analyzing relationships among ciclids fishes, J. Mol. Evol. 53, 89–103.
Freeman, P.W., 1979. Specialized insectivory: beetle-eating and moth-eating molossid bats. J. Mammal. 60, 467–479.
Gippoliti, S., Cotterill, F.P.D., Groves, C.P., 2013. Mammal taxonomy without taxonomists: a reply to Zachos and Lovari, Hystrix 24, 145–147.
González-Ruiz, N., Ramírez-Pulido, J., Arroyo-Cabrales,J., 2011. A new species of mastiff-bat (Chiroptera: Molossidae: Molossus) from Mexico, Mamm. Biol. 76, 461–469.
Gregorin, R., 2003. Comparative anatomy of the tongue in free-tailed bats (Chiroptera, Molossidae), Iheringia Ser. Zool. 93, 213–221.
Gregorin, R., 2009. Phylogeny of Eumops Miller, 1906 (Chiroptera: Molossidae) using morphological data, ActaChiropterol. 11, 247–258.
Gregorin, R., Cirranello, A., 2015. Phylogeny of Molossidae Gervais (Mammalia: Chiroptera) inferred by morphological data. Cladistics 31, https://doi.org/10.1111/cla.12117
Groves, C.P., 2013. The nature of species: a rejoinder to Zachos et al, Mammal. Biol 78, 7–9.
Hammer, Øyvind, Harper, David A.T., Ryan, Paul D., 2001. Past: paleontological statistics software package for education and data analysis, Palaeontol. Electronica 4, 1–9 https://doi.org/palaeo-electronica.org/20011/past/issue1_01.htm
eller, R., Frandsen, P., Lorenzen, E.D., Siegismund, H.R., 2014. Is diagnosability an indicator of speciation? Response to “Why one century of phenetics is enough? Syst, Biol. 63, 833–837.
Jones, G., van Parijs, S.M., 1993. Bimodal echolocation in pipistrelle bats: are cryptic species present, Proc. R. Soc. Lond. B 251, 119–125.
Kalko, E.K., HandleyJr., CO., 2001. Neotropical bats in canopy: diversity, community structure, and implications for conservation, Plant Ecol. 153, 319–333.
Kimura, M., 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences, J. Mol. Evol. 16, 111–120.
Lanfear, R., Calcott, B., Ho, S.Y.W., Guindon, S., 2012. PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses, Mol. Biol. Evol. 29, 1695–1701.
Lewis, P.O., 2001. A likelihood approach to estimating phylogeny from discrete morphological character data, Syst. Biol. 50, 913–925.
Lim, B.K., 2007. Divergence times and origin of neotropical sheath-tailed bats (tribe Diclidurini) in South America, Mol. Phylogenet. Evol. 45, 777–791.
Medina, C.E., Gregorin, R., Zeballos, H., Zamorra, H.T., Moras, L.M., 2014. A new species of Eumops (Chiroptera: Molossidae) from southwestern Peru, Zootaxa 3878, 19–36.
McDonough, M.M., Ammerman, L.K., Timm, R.M., Genoways, H.H., Larsen, P.A., Baker, R.J., 2008. Speciation within bonneted bats (genus Eumops): the complexity of morphological, mitochondrial, and nuclear data sets in systematics, J. Mammal. 89, 1306–1315.
Moratelli, R., Peracchi, A.L., Dias, D., Oliveira, J.A., 2011. Geographic variation in South American populations of Myotis nigricans (Schinz, 1821) (Chiroptera Vespertilionidae), with description of two new species, Mamm. Biol. 76, 592–607.
Morielle-Versute, E., Varella-Garcia, M., Taddei, V.A., 1996. Karyotypic patterns of seven species of molossid bats (Molossidae Chiroptera), Cytogenet. Cell Gen. 72, 26–33.
Nylander, J.A.A., Ronquist, F., Huelsenbeck, J.P., Nieves-Aldrey, J.L., 2004. Bayesian phylogenetic analysis of combined data, Syst. Biol. 53 (1), 47–67.
Paca, R.C., Acosta, L.H., Aguanta, F.S., 2012. Primer registro de Tonatia bidens (Spix, 1823) (Chiroptera Phyllostomidae), en Bolivia, Chiropr. Neotrop. 18, 1063–1066.
Padial, J.M., Miralles, A., de la Riva, I., Vences, M., 2010. The integrative future of taxonomy, Front. Zool. 7, 1–14.
Pumo, D.E., Finamore, P.S., Franek, W.R., Phillips, C.J., Tarzami, S., Balzarano, D., 1998. Complete mitochondrial genome of a Neotropical fruit bat, Artibeus jamaicensis, and a new hypothesis of the relationships of bats to other eutherian mammals, J. Mol. Evol. 15, 709–717.
Rambaut, A., Drummond, A., 2003. Tracer MCMC Trace Analysis Too, University of Oxford, Oxford, UK https://doi.org/evolve.zoo.ox.ac.uk/software.html. Reardon, T.B., McKenzie, N.L., Cooper, S.J.B., Applenton, B., Carthew, S., Adams, M., 2014. A molecular and morphological investigation of species boundaries and phylogenetic relationships in Australian free-tailed bats Mormopterus (Chiroptera: Molossidae). Aust. J. Zool. 62, 109–136.
Redondo, R.A.F., Brina, L.P.S., Silva, R.F., Ditchfield, A.D., Santos, F.R., 2008. Molecular systematics of the genus Artibeus (Chiroptera: Phyllostomidae), Mol. Phyl. Evol. 49, 44–58.
Ryan, J.M., 1991a. Morphology of the glans penis in four genera of molossid bats (Chiroptera: Molossidae). J. Mammal. 72, 658–668.
Ryan, J.M., 1991b. Comparative morphology of the glans penis in Molossus, Promops, and Eumops (Chiroptera, Molossidae). Bull. Am. Mus. Nat. Hist. 432, 122–137.
Sambrook, J., Russell, D.W., Sambrook, J., 2001. Molecular Cloning: A Laboratory Manual, third ed. CSHL press, New York, USA.
Simmons, N.B., Seymour, K.L., Habersetzer,J., Gunnell, G.F., 2008. Primitive early Eocene bat from Wyomming and the evolution of flight and echolocation, Nature 451, 818–821.
Springer, M.S., Stanhope, M.J., Madsen, O., de Jong, W.W., 2004. Molecules consolide the placental mammal tree, TREE 19, 430–438.
Staaden, M.J.V., Jones Jr., J.K., 1997. Comparative morphology of dorsal hair of New World bats of the family Molossidae. In: Arroyo-Cabrales, J., Polaco, O.J. (Eds.), Homenaje al ProfesorTicul Álvarez. Instituto Nacional de Antropología e Historia, Mexico, pp. 373–391.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S., 2011. MEGA5 Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods, Mol. Biol. Evol. 28, 2731–2739.
Tavares, V.C., Perini, F.A., Lombardi, J.A., 2007. The bat communities (Chiroptera) of the Parque Estadual do Rio Doce, a large remnant of Atlantic Forest in southeastern Brazil, Lundiana 8, 35–47.
Teeling, E.C., Madsen, O., van Den Bussche, R.A., de Jong, W.W., Stanhope, M.J., Springer, M.S., 2002. Microbat paraphyly and the convergence evolution of key innovation in Old World rhinolophid microbats, Proc. Natl. Acad. Sci. 99, 1431–1436.
Thomas, D.W., West, S.D., 1989. Sampling methods for bats. In: Ruggiero, L.F., Carey, A.B. (Eds.), Wildlife-habitat Relationships: Sampling Procedures for Pacific Northwest Vertebrates. Gen. Tech. Rep., U.S. Department of Agriculture, Forest Service, Pacific Northwest Research Station, Portland, p. 20.
Timm, R.M., Genoways, H.H., 2004. The Florida Bonneted Bat, Eumops floridanus (Chiroptera: Molossidae): distribution, morphometrics, systematics, and ecology, J. Mammal. 85, 852–865.
Velazco, P.M., Gardner, A.L., Patterson, B.D., 2010. Systematics of the Platyrrhinus helleri species complex (Chiroptera: Phyllostomidae), with descriptions of two new species, Zool. J. Linn. Soc. 159, 785–812.
Wible, J.R., Novacek, M.J., 1988. Cranial evidence forthe monophyletic origin of bats, Am. Mus. Nov. 2911, 1–19.
Zachos, F.E., Apollonio, M., Bärmann, E.V., Festa-Bianchet, M., Göhlich, U., Habel, J.C., Haring, E., Kruchenhauser, L, Lovari, S., McDevitt, A.D., Pertoldi, C, Rössner, G.E., Sánchez-Villagra, M.R., Scandura, M., Suchentrunk, F., 2013. Species inflaction and taxonomic artifacts—a critical comment on recent trends in mammalian classification, Mammal. Biol. 78, 1–6.
Zachos, E.F., Lovari, S., 2013. Taxonomic inflation and the poverty of Phylogenetic Species Concept - a reply to Gippoliti and Groves, Hystrix 24, 142–144.
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Gregorin, R., Moras, L.M., Acosta, L.H. et al. A new species of Eumops (Chiroptera: Molossidae) from southeastern Brazil and Bolivia. Mamm Biol 81, 235–246 (2016). https://doi.org/10.1016/j.mambio.2016.01.002
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DOI: https://doi.org/10.1016/j.mambio.2016.01.002