Abstract
This paper reports the first study of the variation of the chemical composition and abundance of the essential oil of Croton heliotropiifolius, in four seasons, and the evaluation of its antibacterial activity. Essential oil obtained from leaves of C. heliotropiifolius were analyzed by GC/MS and evaluated against eight bacteria strains by broth microdilution method. The chemical constituents identified were the same in all samples, but with different proportions. The total percentages identified were 96.58% in summer, 92.08% in autumn, 98.44% in winter and 90.78% in spring. The majors constituents are β-caryophyllene, bicyclogermacrene, germacrene-D, limonene and 1,8-cineole. β-Caryophyllene was the major compound in all samples. The results of the antibacterial evaluations showed weak to moderate activity against the analyzed strains. In all analyzes was observed that essential oil sample collected in summer stands out from the others, displaying stronger activity against Gram-positive as Gram-negative bacteria.
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JMTAF and LCA contributed in collecting plant samples and obtaining the essential oils. ECCA and JMTAF drafted the paper and contributed to critical reading of the manuscript. LSC and JRGSA contributed to critical reading of the manuscript. AML and ECCA contributed to chromatographic analysis. FSS designed the study and supervised the laboratory work. APO, AGMP and ALG contributed to microbiological study. All the authors had read the final manuscript and approved the submission.
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de Alencar Filho, J.M.T., da Araújo, L.C., Oliveira, A.P. et al. Chemical composition and antibacterial activity of essential oil from leaves of Croton heliotropiifolius in different seasons of the year. Rev. Bras. Farmacogn. 27, 440–444 (2017). https://doi.org/10.1016/j.bjp.2017.02.004
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DOI: https://doi.org/10.1016/j.bjp.2017.02.004