Abstract
Spondias mombin L., Anacardiaceae, is a plant native of Brazil, where it is known as “cajá”. In order to find a potential application for this native species, the anti-inflammatory and antioxidant effects were investigated. The anti-inflammatory activity was evaluated using the in vivo model carrageenan-induced peritonitis in mice. The in vitro antioxidant potential as well the cytotoxicity against 3T3 fibroblast cells also were evaluated. Through High Performance Liquid Chromatography-diode array detector analysis, an analytic method was developed and validated. It allowed the identification and quantification of ellagic acid and chlorogenic acid in hydroethanolic extract of S. mombin leaves. This extract showed antiinflammatory effect at 100, 200, 300 and 500 mg/kg, however, the ethyl acetate fraction, at 200 mg/kg, showed the highlighted results. Ellagic acid and chlorogenic acid (2.5, 5 and 10mg/kg) also inhibited the leukocyte migration to the site of inflammation. The extract, fractions and compounds showed significant antioxidant potential when evaluated in different assays. The results shown in this work suggest the antiinflammatory potential of the leaf extract of S. mombim on peritonitis model induced by carrageenan, it was also observed antioxidant properties associated with an absence of cytotoxicity in cell culture. Further in vivo studies are required to confirm the anti-inflammatories action of S. mombin and its possible anti-inflammatory mechanisms of action.
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Acknowledgments
The authors acknowledge all participants for their valuable time and commitment to the study. We also thank CNPq for financial support (478661/2010-0). The authors are also grateful to MSc Alan de Araújo Roque responsible for species identification.
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BECVF participated of phytochemical analysis, interpretation of data and drafted the manuscript. MMM participated in the evaluation of anti-inflammatory activity, acquisition and interpretation of data. AHK participated in antioxidant analysis and revised it critically the manuscript. RS participated in its design and coordination, and helped to draft the manuscript.
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Cabral, B., Siqueira, E.M.S., Bitencourt, M.A.O. et al. Phytochemical study and anti-inflammatory and antioxidant potential of Spondias mombin leaves. Rev. Bras. Farmacogn. 26, 304–311 (2016). https://doi.org/10.1016/j.bjp.2016.02.002
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DOI: https://doi.org/10.1016/j.bjp.2016.02.002