Abstract
Laetia suaveolens (Poepp.) Benth., Salicaceae, popularly known as “casinga-cheirosa”, “caferana”, or “laranjinha”, is native to Brazil but not endemic to this country. A crude organic extract was obtained from the leaves and stem and intraperitoneally administered in male Balb-c mice. Its behavioral effects were evaluated in the open field and elevated plus maze in a two-stage experiment that assessed ten different parameters related to behavior as locomotion, emotionality, and anxiety. In the first stage of the experiment, intraperitoneal the crude organic extract administration dose-dependently impaired locomotion and emotionality 30-120 min after administration. A significant decrease in defecation was observed, which was related to emotionality. No alterations in the elevated plus maze were found; thus, this apparatus was not used in the next stage of the experiment. In the second stage, the previously determined non-lethal dose of 0.1563g/kg was intraperitoneally administered, which impaired locomotion and rearing frequency and increased immobility time. Necropsy revealed smooth intestine hemorrhage. Rutin, leucoside, nicotiflorin, guaijaverin, and astragalin were isolated from the crude organic extract. This is the first time that these compounds have been identified in L. suaveolens. In conclusion, the crude organic extract impaired locomotion and emotionality and caused hemorrhage in male Balb-c mice, indicating that its consumption can be harmful to humans and animals. The present results provide a basis for further studies on the pharmacology, toxicology, and natural product chemistry of the crude organic extract.
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The authors thank Fundação de Amparo à Pesquisa do Estado de São Paulo for financial support (grant no. 2008/58706-8) and Michele Sanchez for technical assistance.
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DME (M.Sc. student) and DFG (M.Sc. student) ran the laboratory work. MLBP contributed to plant identification and her barium confection. SAF contributed to plant collection, her barium confection, and the laboratory work. IECD contributed to the chromatographic analysis and molecule identification. LFLR critically read the manuscript and contributed to the HPLC analysis. EFSM contributed to maintaining the animal facilities. MMB and IBS designed the study, supervised the laboratory work, wrote the manuscript, performed the laboratory work, and critically read the manuscript. All of the authors read the final manuscript and approved the submission.
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Estork, D.M., Gusmão, D.F., Paciencia, M.L.B. et al. Casinga-cheirosa organic extract impairment over Balb-c male mice behavioral phenotype. Revista Brasileira de Farmacognosia 26, 216–224 (2016). https://doi.org/10.1016/j.bjp.2015.10.004
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DOI: https://doi.org/10.1016/j.bjp.2015.10.004