Skip to main content
SpringerLink
Log in

Plasmon-based colorimetric assay using green synthesized gold nanoparticles for the detection of bisphenol A

  • Original Paper
  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

Herein, we report a green synthesized gold nanoparticle (AuNPs) based colorimetric detection of bisphenol A (BPA). The AuNPs were synthesized using khat leaf extract as a reducing agent by optimizing factors affecting the AuNPs synthesis, including gold precursor concentration (1 mM), and reaction temperature (60 °C). The AuNPs characterization was carried out using ultraviolet–visible spectrophotometry and transmission electron microscopy, and it was found spherical with an average particle size of 17.3 ± 3.7 nm. A colorimetric nanosensor was developed by conjugation of bio-inspired AuNPs with BPA-specific aptamer for a quick and easy detection of BPA in plastic bottled water. The colorimetric assay relies on the strong affinity of BPA for aptamer, which causes detachment of the aptamer from the AuNPs surface in the presence of BPA inducing AuNPs aggregation. To achieve the colorimetric detection of BPA, the concentrations of NaCl and aptamer were optimized. The detection of BPA was monitored visually using a naked eye, as well as quantitatively using an ultraviolet–visible spectrophotometer. The method visual limit of detection (LOD) was determined to be 0.1 ng/mL and reached 0.09 ng/mL using ultraviolet–visible spectrophotometer. The method demonstrated very good linearity (R2 = 0.9986) in the range of 0.1–100 ng/mL. The proposed method showed high sensitivity to BPA detection in plastic bottled water with 86.7–98.0%, recovery. Therefore, the proposed colorimetric nanosensor can be used for determination of BPA in plastic bottled waters with reliable performance at lower concentrations.

Graphical abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Availability of data and materials

All data generated in the present research are included in this published article. The data and materials presented in this article are of availability.

References

  1. Y.-Y. Fan, J.-L. Zheng, J.-H. Ren, J. Luo, X.-Y. Cui, L.Q. Ma, Environ. Pollut. 192, 113–120 (2014)

    Article  CAS  PubMed  Google Scholar 

  2. J. Rajasärkkä, M. Pernica, J. Kuta, J. Lašňák, Z. Šimek, L. Bláha, Water Res. 103, 133–140 (2016)

    Article  PubMed  Google Scholar 

  3. T.A. Cooper, Trends in Packaging of Food, Beverages and Other Fast-Moving Consumer Goods (FMCG) (Elsevier, Amsterdam, 2013), pp.58–107

    Book  Google Scholar 

  4. R. Banaderakhshan, P. Kemp, L. Breul, P. Steinbichl, C. Hartmann, M. Fürhacker, Chemosphere 286, 131842 (2022)

    Article  CAS  PubMed  Google Scholar 

  5. Z. Hu, L.W. Morton, R.L. Mahler, Int. J. Environ. Res. Public Health 8, 565–578 (2011)

    Article  PubMed  PubMed Central  Google Scholar 

  6. A. Agarwal, S. Gandhi, A.D. Tripathi, M. Iammarino, S. Homroy, Int. J. Food Sci. Technol. 57, 7477–7485 (2022)

  7. S. Almeida, A. Raposo, M. Almeida-González, C. Carrascosa, Comp. Rev. Food Sci. Food Saf. 17, 1503–1517 (2018)

    Article  Google Scholar 

  8. M. Benhamada, D. Bouzid, O. Boyron, M. Taam, Eur. Food Res. Technol. 242, 227–232 (2016)

    Article  CAS  Google Scholar 

  9. P. Fenichel, N. Chevalier, F. Brucker-Davis, Ann. Endocrinol. 74, 211–220 (2013)

    Article  CAS  Google Scholar 

  10. M.-L. Jugan, Y. Levi, J.-P. Blondeau, Biochem. Pharmacol. 79, 939–947 (2010)

    Article  CAS  PubMed  Google Scholar 

  11. Y. Jiang, T. Tang, Z. Cao, G. Shi, T. Zhou, J. Sep. Sci. 38, 2158–2166 (2015)

    Article  CAS  PubMed  Google Scholar 

  12. Y. Niu, J. Zhang, Y. Wu, B. Shao, J. Agric. Food Chem. 60, 6116–6122 (2012)

    Article  CAS  PubMed  Google Scholar 

  13. B. Liu, J. Zhuang, G. Wei, Environ. Sci. NANO 7, 2195–2213 (2020)

    Article  CAS  Google Scholar 

  14. K. Saha, S.S. Agasti, C. Kim, X. Li, V.M. Rotello, Chem. Rev. 112, 2739–2779 (2012)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. N. Passarelli, L.A. Pérez, E.A. Coronado, ACS Nano 8, 9723–9728 (2014)

    Article  CAS  PubMed  Google Scholar 

  16. K.A. Willets, R.P. Van Duyne, Annu. Rev. Phys. Chem. 58, 267–297 (2007)

    Article  CAS  PubMed  Google Scholar 

  17. R. Elghanian, J.J. Storhoff, R.C. Mucic, R.L. Letsinger, C.A. Mirkin, Science 277, 1078–1081 (1997)

    Article  CAS  PubMed  Google Scholar 

  18. Q. Zhao, H. Huang, L. Zhang, L. Wang, Y. Zeng, X. Xia, F. Liu, Y. Chen, Anal. Chem. 88, 1412–1418 (2016)

    Article  CAS  PubMed  Google Scholar 

  19. Y. Zhu, Y. Cai, L. Xu, L. Zheng, L. Wang, B. Qi, C. Xu, ACS Appl. Mater. Interfaces 7, 7492–7496 (2015)

    Article  CAS  PubMed  Google Scholar 

  20. J. Xu, Y. Li, J. Bie, W. Jiang, J. Guo, Y. Luo, F. Shen, C. Sun, Microchim. Acta 182, 2131–2138 (2015)

    Article  CAS  Google Scholar 

  21. L. Chen, J. Li, L. Chen, ACS Appl. Mater. Interfaces 6, 15897–15904 (2014)

    Article  CAS  PubMed  Google Scholar 

  22. Y. Wang, F. Yang, X. Yang, ACS Appl. Mater. Interfaces 2, 339–342 (2010)

    Article  CAS  PubMed  Google Scholar 

  23. C. Coman, L.F. Leopold, O.D. Rugină, L. Barbu-Tudoran, N. Leopold, M. Tofană, C. Socaciu, J. Nanopart. Res. 16, 2158 (2013)

    Article  Google Scholar 

  24. S. Khalililaghab, S. Momeni, M. Farrokhnia, I. Nabipour, S. Karimi, Anal. Bioanal. Chem. 409, 2847–2858 (2017)

    Article  CAS  PubMed  Google Scholar 

  25. Z. Mei, H. Chu, W. Chen, F. Xue, J. Liu, H. Xu, R. Zhang, L. Zheng, Biosens. Bioelectron. 39, 26–30 (2013)

    Article  CAS  PubMed  Google Scholar 

  26. N.T. Wabe, Addict. Health. 3, 137 (2011)

    PubMed  PubMed Central  Google Scholar 

  27. A.M. El Shafey, Green Process. Synth. 9, 304–339 (2020)

    Article  Google Scholar 

  28. M. Khoshnamvand, S. Ashtiani, C. Huo, S.P. Saeb, J. Liu, J. Mol. Struct. 1179, 749–755 (2019)

    Article  CAS  Google Scholar 

  29. B. Refera, D. Bekana, A. Temesgen, E. Teju, A. Jabessa, Res. Chem. Intermed. 49, 3227–3244 (2023)

    Article  CAS  Google Scholar 

  30. H.I. Salaheldin, M.H. Almalki, A.E.M. Hezma, G.E. Osman, IET Nanobiotechnol. 11, 448–453 (2017)

    Article  PubMed  PubMed Central  Google Scholar 

  31. M. Tesfaye, Y. Gonfa, G. Tadesse, T. Temesgen, S. Periyasamy, Heliyon 9(6), e17356 (2023)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. P. Balashanmugam, P. Durai, M.D. Balakumaran, P.T. Kalaichelvan, J. Photochem. Photobiol. B. 165, 163–173 (2016)

    Article  CAS  PubMed  Google Scholar 

  33. K. Sneha, M. Sathishkumar, S. Kim, Y.-S. Yun, Process Biochem. 45, 1450–1458 (2010)

    Article  CAS  Google Scholar 

  34. P.A. Mirau, J.E. Smith, J.L. Chávez, J.A. Hagen, N. Kelley-Loughnane, R. Naik, Langmuir 34, 2139–2146 (2018)

    Article  CAS  PubMed  Google Scholar 

  35. L. Huang, W. Xin, L. Sha, G. Zhi-Xian, Chin. J. Anal. Chem. 49, 172–183 (2021)

    Article  CAS  Google Scholar 

  36. E.-H. Lee, S.K. Lee, M.J. Kim, S.-W. Lee, Food Chem. 287, 205–213 (2019)

    Article  CAS  PubMed  Google Scholar 

  37. European Food Safety Authority (EFSA), Report on the Two-Phase Public Consultation on the Draft EFSA Scientific Opinion On Bisphenol A (BPA). Wiley Online Library (2015)

  38. B.S. Rubin, J. Steroid Biochem. Mol. Biol. 127, 27–34 (2011)

    Article  CAS  PubMed  Google Scholar 

  39. D. Yao, G. Wen, Z. Jiang, RSC Adv. 3, 13353–13356 (2013)

    Article  CAS  Google Scholar 

  40. N. Yildirim, F. Long, M. He, H.-C. Shi, A.Z. Gu, Environ Sci Process Impacts 16, 1379–1386 (2014)

    Article  CAS  PubMed  Google Scholar 

  41. M.A. Rahman, M.J. Shiddiky, J.-S. Park, Y.-B. Shim, Biosens. Bioelectron. 22, 2464–2470 (2007)

    Article  CAS  PubMed  Google Scholar 

  42. Y. Qiao, J. Li, H. Li, H. Fang, D. Fan, W. Wang, Biosens. Bioelectron. 86, 315–320 (2016)

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The authors gratefully acknowledge the financial support provided by Haramaya University Office of Research Affairs (HURG_2019_03_02_90).

Author information

Authors and Affiliations

  1. Department of Chemistry, College of Natural and Computational Sciences, Haramaya University, Haramaya, Ethiopia

    Zinet Adem, Deribachew Bekana, Ayalew Temesgen, Endale Teju & Meseret Amde

  2. Department of Chemical Engineering, Haramaya Institute of Technology, Haramaya University, Haramaya, Ethiopia

    Abdisa Jabesa

Authors
  1. Zinet Adem
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Deribachew Bekana
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ayalew Temesgen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Endale Teju
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Meseret Amde
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Abdisa Jabesa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Deribachew Bekana.

Ethics declarations

Conflict of interest

The authors declare no competing financial interest.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Adem, Z., Bekana, D., Temesgen, A. et al. Plasmon-based colorimetric assay using green synthesized gold nanoparticles for the detection of bisphenol A. ANAL. SCI. 40, 671–679 (2024). https://doi.org/10.1007/s44211-023-00500-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s44211-023-00500-8

Keywords

Search

Navigation