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Highly sensitive detection of thyroglobulin based on sandwich-type electrochemical immunoassay

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Abstract

As a dimeric protein, thyroglobulin (Tg) is an important biomarker for different thyroid cancer (DTC), so designing effective method to detect Tg is of great significance. In this work, by preparing β-cyclodextrin (CD) functionalized carbon nanotubes (CNTs) nanohybrid (CD-CNTs) as carrier to immobilize primary antibody (Ab1) of Tg, assembling sulfydryl ferrocene (Fc) and secondary antibody (Ab2) on the surface of nanogold (Au) as signaling amplifier (Ab2-Au-Fc), a simple and sensitive sandwich-type electrochemical immunoassay (STEM) of Tg was designed herein for the first time. In brief, CNTs show large surface area and conductivity, while CD offers superior host–guest recognition capability that can bound with Ab1; meanwhile, Fc probe can offer stable electrochemical signal that is proportionable to the concentration of Tg. Under the optimum conditions, the proposed STEM platform shows excellent sensing results for Tg detection: a considerable low analytical detection (0.5 ng mL−1) and wide linearity (2 to 200 ng mL−1), suggesting the designed STEM platform offers potential real applications for detect Tg.

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Acknowledgements

The work was financially supported by 2022 project support fund Department of Finance of Fujian Provincial (MCZ [2021] No. 848).

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Author notes

  1. Huizhen Zhang and Zhiyi Lin have contributed equally to this work.

Authors and Affiliations

  1. Department of Nuclear Medicine, Fujian Provincial Hospital, Shengli Clinical Medical College of Fujian Medical University, Fuzhou, 350001, China

    Zhiyi Lin, Yuxuan Li, Ziyi Lin, Shengping Yang & Mingdian Yu

  2. Department of B-Mode Ultrasound, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial, Fuzhou, 350001, China

    Huizhen Zhang

  3. Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology (Fuzhou University), Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou, Fujian, 350108, China

    Bin Qiu

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  1. Huizhen Zhang
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Correspondence to Bin Qiu or Mingdian Yu.

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Zhang, H., Lin, Z., Li, Y. et al. Highly sensitive detection of thyroglobulin based on sandwich-type electrochemical immunoassay. ANAL. SCI. 39, 969–975 (2023). https://doi.org/10.1007/s44211-023-00305-9

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  • DOI: https://doi.org/10.1007/s44211-023-00305-9

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