Abstract
Cell proliferation and migration can be triggered by an injury where fibroblasts play a vital role in the repair processes. Extracts of Moringa oleifera Lam., Moringaceae, containing bioactive substances, have been used for healing of both wounds and burns. The objective of this study was to promote the proliferation and cellular migration of fibroblasts with a methanolic extract of M. oleifera leaves, obtained by sonication. Additionally, the bioactive constituents were dereplicated by high-performance liquid chromatography–mass spectrometry. The effect of leaves methanolic extract on cell proliferation and migration (wound closure) in a human skin fibroblast cell line was assessed. At a low concentration (0.01 µg/ml), the extract promoted a significant increased rate of cell proliferation and migration. Flavonols and flavone glycosides were identified as the main group of compounds which contributed to this biological activity. The methanolic extract from M. oleifera leaves can have a positive effect on the processes of proliferation and migration during wound healing.
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Acknowledgements
We would like to thank the Laboratory No. 11 of stem cell culture of the Faculty of Medicine UAEM, Morelos, for the support in the culture of human skin fibroblasts. We are grateful to the Spectrophotometry and Chromatography Laboratory of CEPROBI-IPN, for the support given in HPTLC analysis. Thanks to the Center for Nanosciences and Micro and Nanotechnologies (CNMN-IPN) for the technical support of HPLC–MS and the BEIFI Program. The author Rodríguez-García received a scholarship from the Consejo Nacional de Ciencia y Tecnología (CONACYT).
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Financial support was provided by Instituto Politécnico Nacional (IPN-México) through Project SIP 20196636, SIP-20190032.
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Conceptualization, investigation, and manuscript writing, review, and editing: TRG; supervision and project administration: ARJA; methodology and formal analysis: BHCD; resources and methodology: JST; conceptualization and writing of original draft: SEL; funding acquisition, conceptualization, and manuscript writing and editing: MLAO. All authors read and approved the final manuscript.
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Rodríguez-García, T., Camacho-Díaz, B.H., Jiménez–Aparicio, A.R. et al. Cell Proliferation and Migration in Human Skin Fibroblasts Induced by Moringa oleifera. Rev. Bras. Farmacogn. 31, 302–309 (2021). https://doi.org/10.1007/s43450-021-00160-7
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DOI: https://doi.org/10.1007/s43450-021-00160-7