Abstract
Breast cancer is an invasive malignant neoplasm; however, an increasing number of naturally occurring chemical compounds have been identified as potent cytotoxic agents. Kadsura coccinea (Lem.) A.C.Sm., Schisandraceae, is a medicinally important Chinese plant. Schisandronic acid, a cycloartane triterpenoid, is a major cytotoxic compound from this plant. The mechanisms behind the cytotoxic potential of schisandronic acid against a human breast carcinoma cell line were evaluated by employing cell viability assay using MTT and western blot analysis. Antioxidant capacity was measured by employing luminol- and lucigenin-enhanced chemiluminescence assays. Schisandronic acid was able to induce cell apoptosis in MCF-7 cells in a time-dependent manner. This triterpenoid significantly reduced cell viability in a concentration-dependent manner with an IC50 value of 8.06 ± 1.119 μM. The mechanisms might be mediated through upregulation of the expression of active caspase-3, and cleavage of poly (ADP-ribose) polymerase. Furthermore, schisandronic acid was investigated for its antioxidant effects and shown reduced generation of reactive oxygen species.
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Funding
Financial support was provided by the Hunan Province Universities 2011 collaborative Innovation Center of Protection and Utilization of Huxiang Chinese Medicine Resources, Hunan Provincial Key Laboratory of Diagnostics in Chinese Medicine and National Natural Science Foundation of China (81673579 and 81874369), Talented Young Scientist Program for developing countries, Ministry of Science and Technology, China, and Shaanxi innovative talents promotion plan and technological innovation team (2018TD-005).
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Biological evaluations and writing of the manuscript: ST; chemical analysis as well as writing the chemistry part: YY; supervision of all activities and biological assays: BL; preparation of the whole project and chemical analysis: MIC; design of the study, provision of plant material, supervision of the whole project and critical review of the manuscript: WW; all authors have approved the final article.
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Tasneem, S., Yang, Y., Liu, B. et al. Cytotoxicity of Schisandronic Acid from Kadsura coccinea by Activation of Caspase-3, Cleavage of poly-ADP Ribose Polymerase, and Reduction of Oxidative Stress. Rev. Bras. Farmacogn. 31, 51–58 (2021). https://doi.org/10.1007/s43450-021-00136-7
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DOI: https://doi.org/10.1007/s43450-021-00136-7