Abstract
Coumarins from Pterocaulon balansae Chodat, Asteraceae, have received increasing attention due to their biological activities, especially for the treatment of skin disorders. This study aimed to develop and validate high-performance and ultra-fast liquid chromatography methods for the analytical determination of coumarins in extracts, in topical formulations, as well as in porcine skin after in vitro permeation/retention studies. The chromatographic conditions consisted of a mobile phase with 0.1% formic acid (A) and acetonitrile (B) using a gradient elution; the flow rate and a column oven temperature: HPLC-UV, 1 ml min−1; 30 °C and UFLC-DAD, 0.55 ml min−1; 55 °C for 45 min and 8 min, respectively. The chemical marker 5-methoxy-6,7-methylenedioxycoumarin was used for the construction of the linearity curve in a concentration range from 0.1 to 7.5 μg ml−1. A low matrix effect was observed. The method robustness was confirmed using the Plackett-Burman experimental design. These methodologies proved to be linear, precise, and accurate for the determination of seven coumarins in analytical and bioanalytical samples in both approaches. However, UFLC analysis time was shortened to about one-fourth the time and consumes significantly less eluent, being more eco-friendly in comparison with HPLC.
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Acknowledgments
BMN would like to thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil) for a scholarship (finance code 001). HT and GVP are recipients of research fellowship from The Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
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Financial support was received from the Brazilian agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS).
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BMN contributed to plant collection and identification, confection of herbarium, running the laboratory work, analysis of the data, and drafting the paper. MCN and NTB contributed to the laboratory work and analysis of the data. RSS and MS contributed to critical reading of the manuscript. HT and GVP designed the study, supervised the laboratory work, and contributed to critical reading of the manuscript. All authors have read the final manuscript and approved the submission.
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Medeiros-Neves, B., Nemitz, M.C., Brazil, N.T. et al. Determination of Coumarins from Pterocaulon balansae by an Ultra-Fast Liquid Chromatography Method in Topical Applications. Rev. Bras. Farmacogn. 30, 765–773 (2020). https://doi.org/10.1007/s43450-020-00115-4
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DOI: https://doi.org/10.1007/s43450-020-00115-4