Abstract
This present study aims to develop a liquid chromatography coupled to tandem mass spectrometry assay to quantify phillyrin in rat plasma and tissues. Plasma and tissue samples were processed via protein precipitation, and calceolarioside B was chosen as the internal standard. Selected reactions monitoring mode was applied to detect the analytes of all samples. Mass spectrometry was conducted in the negative ion mode for detection. The MS/MS ion transitions monitored were m/z 533.4 → 370.8 for phillyrin and m/z 477.3 → 161.0 for calceolarioside B, respectively. All calibration curves were linear over the range of 1–800 ng/ml in each biological matrix. The lower limit of quantification for plasma and tissue homogenates was 1 ng/ml. Inter- and intra-day precision and accuracy well met within the acceptable ranges (≤ 15%). It was successfully applied to the pharmacokinetic and tissue distribution studies of phillyrin after intravenous doses of 0.1 mg/kg in rats.
Graphical Abstract
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
An H, Bai Y (2019) Mahuang Lianqiao Chixiaodou Tang in the treatment for children with acute glomerular nephritis: a report of 34 cases. Western J Trad Chin Med 32:58–60
Bai Y, Li J, Liu W, Jiao XC, He J, Liu J, Ma L, Gao X, Chang YX (2015) Pharmacokinetic of 5 components after oral administration of Fructus Forsythiae by HPLC-MS/MS and the effects of harvest time and administration times. J Chromatogr B 993-994:36–46. https://doi.org/10.1016/j.jchromb.2015.04.041
Feng SX, Li XH, Wang MM, Hao R, Li MM, Zhang L, Wang Z (2018) A sensitive HPLC-MS method for simultaneous determination of thirteen components in rat plasma and its application to pharmacokinetic study of Tanreqing injection. J Pharm Biomed Anal 148:205–213. https://doi.org/10.1016/j.jpba.2017.10.006
Han Z, Lei XL, Zhang H, Liu L, Chen ZS, Yang W, Lun ZR (2017) Evaluating the safety of forsythin from Forsythia suspensa leaves by acute and sub-chronic oral administration in rodent models. Asian Pac J Trop Med 10:47–51. https://doi.org/10.1016/j.apjtm.2016.10.011
Jiang Q, Chen J, Long X, Yao X, Zou X, Yang Y, Huang G, Zhang H (2020) Phillyrin protects mice from traumatic brain injury by inhibiting the inflflammation of microglia via PPARγ signaling pathway. Int Immunopharmacol 79:106083. https://doi.org/10.1016/j.intimp.2019.106083
Kong P, Zhang L, Guo Y, Lu Y, Lin D (2014) Phillyrin, a natural lignan, attenuates tumor necrosis factor α-mediated insulin resistance and lipolytic acceleration in 3T3-L1 adipocytes. Planta Med 80:880–886. https://doi.org/10.1055/s-0034-1368614
Li H, Chen F (2005) Preparative isolation and purifification of phillyrin from the medicinal plant Forsythia suspensa by high-speed counter-current chromatography. J Chromatogr A 1083:102–105. https://doi.org/10.1016/j.chroma.2005.06.025
Li Y, Jiang X, Huang L, Lu J, Zhou J (2006) Determination of phillyrin in rat plasma by high-performance liquid chromatography and its application to pharmacokinetic studies. J Pharm Biomed Anal 41:628–632. https://doi.org/10.1016/j.jpba.2005.12.006
Li Y, Peng C, Zhang R, Li X, Jiang X (2010) Simultaneous determination of phillyrin and forsythiaside in beagle dog plasma by high-performance liquid chromatography with gradient elution and its application to pharmacokinetic studies. Anal Lett 43:209–218. https://doi.org/10.1080/00032710903325765
Liu X, Yu S, Huo H, Li F, Sheng N, Zhang L (2013) HPLC-ESI-MS/MS quantitative method for simultaneous analysis of five bioactive constituents of Forsythia suspensa in rat bile after oral administration of forsythia suspensa extract. J Liq Chromatogr R T 36:44–60. https://doi.org/10.1080/10826076.2011.644051
National Commission of Chinese Pharmacopoeia (2015) Pharmacopoeia of Peoples Republic of China. Chemical Industry Press, Beijing
Qu H, Li B, Li X, Tu G, Lu J, Sun W (2008a) Qualitative and quantitative analyses of three bioactive compounds in different parts of Forsythia suspensa by high-performance liquid chromatography electrospray ionization-mass spectrometry. Microchem J 89:159–164. https://doi.org/10.1016/j.microc.2008.02.002
Qu H, Zhang Y, Wang Y, Li B, Sun W (2008b) Antioxidant and antibacterial activity of two compounds (forsythiaside and forsythin) isolated from Forsythia suspensa. J Pharm Pharmacol 60:261–266. https://doi.org/10.1211/jpp.60.2.0016
Wang G, Li R, Zhang X (2009) Pharmacokinetics of two ingredients of the compound Chinese herbal medicine transdermal preparation in cows. Agric Sci China 8:1138–1142. https://doi.org/10.1016/S1671-2927(08)60322-3
Xiao HB, Sui GG, Lu XY (2018) Phillyrin lowers body weight in obese mice via the modulation of PPAR<beta>/<delta>-ANGPTL 4 pathway. Obes Res Clin Pract 12:71–79. https://doi.org/10.1016/j.orcp.2017.02.002
Ye J, Song X, Liu Z, Zhao X, Geng L, Bi K, Chen X (2012) Development of an LC-MS method for determination of three active constituents of Shuang-huang-lian injection in rat plasma and its application to the drug interaction study of Shuang-huang-lian freeze-dried powder combined with levoflfloxacin injection. J Chromatogr B 898:130–135. https://doi.org/10.1016/j.jchromb.2012.04.036
Zhao YM, Li FR, Yang JX, Liang J, Zhang LS (2005) Study on the reducing blood lipid and antioxidation effects of phillyrin. Nat Prod Res 17:157–159. https://doi.org/10.16333/j.1001-6880.2005.02.008
Zheng W, Zhuo R (2006) Mahuang Lianqiao Chixiaodou Tang in the treatment for children with acute glomerular nephritis: a report of 38 cases. Fujian Med J 28:136–137
Zhong WT, Wu YC, Xie XX, Zhou X, Wei MM, Soromou LW, Ci XX, Wang DC (2013) Phillyrin attenuates LPS-induced pulmonary inflflammation via suppression of MAPK and NF-κB activation in acute lung injury mice. Fitoterapia 90:132–139. https://doi.org/10.1016/j.fitote.2013.06.003
Author information
Authors and Affiliations
Contributions
HW wrote the manuscript. SY carried out the data analyses. QX designed the study and supervised the laboratory work. All authors read the manuscript and approved the submission.
Corresponding author
Ethics declarations
Conflict of Interest
The authors declare that they have no conflicts of interest.
Electronic Supplementary Material
ESM 1
(PDF 287 kb).
Rights and permissions
About this article
Cite this article
Wang, H., Yang, S. & Xian, Q. Pharmacokinetics and Tissue Distribution of Phillyrin in Rats. Rev. Bras. Farmacogn. 30, 818–823 (2020). https://doi.org/10.1007/s43450-020-00100-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s43450-020-00100-x