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Simultaneous Determination of Five Components of Laportea bulbifera Extract in Rat Plasma by LC-MS/MS: Application to a Pharmacokinetic Study

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Abstract

Laportea bulbifera (Siebold & Zucc.) Wedd., Urticaceae, is a medicinal herb customarily used by the Miao people in China, for the treatment of rheumatism, traumatic injury, and other symptoms. The aim of this study was to develop and validate a sensitive and reliable high-performance liquid chromatography-tandem mass spectrometry method for the simultaneous quantitation of five constituents, including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, and kaempferol-3-O-rutinoside, of L. bulbifera extract in biological samples, and to apply the method to a pharmacokinetic study. The validated method was successfully applied to a pharmacokinetic study in rats with oral administration of L. bulbifera extract at a single dose of 8.3 g kg−1. The five constituents were detected and their pharmacokinetic parameters were calculated. The established method was rapid and sensitive and could be used for the pharmacokinetic study of L. bulbifera extract.

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Acknowledgments

The authors would like to express their sincere thanks to Dr. Laura Urbano from the University of Hertfordshire for her improvement of the writing in this manuscript. Moreover, we thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

Funding

This research was financially supported by the National Natural Science Foundation of China (81560693, U1812403), Guizhou Science and Technology Department Plat form Talent Project (20165613/5677), Guizhou Youth Project ((2017)5601), and Guiyang Science and Technology Department Platform Talent Project ((2017)30-29).

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Author notes

  1. Siying Chen and Juan Tang contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou Medical University, Guiyang, China

    Siying Chen, Juan Tang, Yueting Li, Yong Huang, Lin Zheng, Jie Pan, Zipeng Gong & Yonglin Wang

  2. Guizhou Provincial Engineering Research Center for the Development and Application of Ethnic Medicine and TCM, Guizhou Medical University, Guiyang, China

    Dan Wu & Yanyu Lan

  3. School of Pharmacy, Guizhou Medical University, Guiyang, China

    Juan Tang & Dan Wu

Authors
  1. Siying Chen
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  2. Juan Tang
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  3. Dan Wu
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Contributions

SYC and JT contributed equally to this work. SYC, ZPG, and YLW conceived and designed the experiments. SYC and JT performed all of the experiments. DW, YTL, YH, LZ, JP, YYL, AMW, and YJL contributed operation of rats/reagents/materials/analysis tools. SYC and JT analyzed the data and wrote a draft of the paper. ZPG and YLW contributed to critical review of the paper.

Corresponding authors

Correspondence to Zipeng Gong or Yonglin Wang.

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This is an original research and has not been sent in any form for publication to other journals. The data presented in the manuscript are originally collected by the research activity at the laboratory. This is the first time to you. Before submitting the revised manuscript, the authors of the manuscript have read the paper and revised it under their helps for many times; at last, we agreed to submit the manuscript to Revista Brasileira de Farmacognosia.

Conflict of Interest

The authors declare that they have no conflict of interest.

Protection of Human and Animal Subjects

The authors declare that no experiments were performed on humans. And all studies of animal experiments were approved by the Animal Ethics Committee at Guizhou Medical University (No. 1503017) and conducted in accordance with the guidelines of the Committee on the Care and Use of Laboratory Animals in China.

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Chen, S., Tang, J., Wu, D. et al. Simultaneous Determination of Five Components of Laportea bulbifera Extract in Rat Plasma by LC-MS/MS: Application to a Pharmacokinetic Study. Rev. Bras. Farmacogn. 30, 61–68 (2020). https://doi.org/10.1007/s43450-020-00013-9

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