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Efficient production of (2R, 3R)-butanediol from xylose by an engineered Serratia marcescens

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Abstract

(2R,3R)-BDO is an important bio-based four-carbon platform compound and widely used in chemical, food, pharmaceutical, fuel and aerospace fields. Serratia marcescens has been shown to be an effective strain for producing BDO. However, there have been few reports on the metabolic engineering of this strain to efficiently produce (2R,3R)-BDO using xylose. In this study, the endogenous strong promoter was screened and modified, and combined with the screened RBS. (2R,3R)-BDO was successfully produced by combining the selected promoters and RBS with the overexpressed key enzymes (ALS, ALSD, BDH, GLF) of (2R,3R)-BDO production. The optimum fermentation temperature and pH were 37 ℃ and 6.0, respectively, and the optimized yield reached 6.0 g/L. After batch fermentation, the (2R,3R)-BDO yield reached 36.6 g/L. This provided a good idea for efficient production of (2R,3R)-BDO from xylose.

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Acknowledgements

Thank all the authors for their contributions to this article.

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Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China

    Tingting Sun, Di Liu, Linmeng Zhang & Yujie Cai

  2. Department of Pharmacology, University of Cambridge, Cambridge, CB2 1PD, UK

    Taiping Fan

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  1. Tingting Sun
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  2. Di Liu
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  3. Linmeng Zhang
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TS: investigation, visualization, writing—original draft. DL and LZ: methodology, investigation, data curation, formal analysis. YC: conceptualization, supervision, writing—review and editing.

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Correspondence to Yujie Cai.

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Sun, T., Liu, D., Zhang, L. et al. Efficient production of (2R, 3R)-butanediol from xylose by an engineered Serratia marcescens. Syst Microbiol and Biomanuf 4, 820–830 (2024). https://doi.org/10.1007/s43393-023-00219-7

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