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Chemerin/CMKLR1 pathway exacerbates cisplatin-induced spiral ganglion neuron injury

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Abstract

This study investigated whether chemerin/chemokine-like receptor 1 (CMKLR1) pathway participate in cisplatin‐induced spiral ganglion neuron (SGN) damage. Middle cochlear turn was collected from C57BL/6 mice and the SGNs were cultured. Cisplatin, 2-(anaphthoyl) ethyltrimethylammonium iodide (α-NETA), or recombinant mouse chemerin was added into the medium for the treatment. Relative mRNA and protein expression was determined by RT-PCR, ELISA and Western blot, respectively. In cultured mouse cochlear SGNs, the treatment of cisplatin enhanced the secretion of chemerin and CMKLR1. Recombinant chemerin promoted but α-NETA inhibited chemerin/CMKLR1 pathway in cisplatin stimulated SGNs. Cisplatin-induced apoptosis and inflammation response in SGNs were enhanced by recombinant chemerin while inhibited by α-NETA. Recombinant chemerin promoted but α-NETA inhibited NF-κB signal in cisplatin stimulated SGNs. In conclusion, chemerin/CMKLR1 pathway regulated apoptosis and inflammation response in cisplatin-induced SGN injury through NF-κB signaling pathway.

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The data could be obtained upon reasonable request to the corresponding author.

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Authors and Affiliations

  1. Department of Otology, Zibo Central Hospital, No. 54, Gongqingtuan West Road, Zhangdian District, Zibo, 255036, Shandong, China

    Jie Tian

  2. Department of Emergency Medicine, Zibo Central Hospital, No. 54 Gongqingtuan West Road, Zibo, 255036, Shandong, China

    Ying Mu

  3. Department of Neurology, Zibo Central Hospital, No. 54 Gongqingtuan West Road, Zibo, 255036, Shandong, China

    Lili Ma

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Tian, J., Mu, Y. & Ma, L. Chemerin/CMKLR1 pathway exacerbates cisplatin-induced spiral ganglion neuron injury. Toxicol Res. 40, 73–81 (2024). https://doi.org/10.1007/s43188-023-00205-0

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