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Evaluation of genotoxicity of SUNACTIVE Zn-P240 in vitro and in vivo

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Abstract

We evaluated the potential genotoxic effects of the nutrient supplement SUNACTIVE Zn-P240 in vitro and in vivo. Genotoxicity tests were performed at the Korea Testing and Research Institute, a GLP certification institution. A bacterial reverse mutation test was performed using the pre-incubation method, while the in vitro chromosome aberration test was performed using a cultured Chinese hamster lung cell line in the presence or absence of metabolic activation. The in vivo micronucleus test was performed using ICR mice. The bacterial reverse mutation test revealed that SUNACTIVE Zn-P240 did not induce genetic mutations at the tested doses in Salmonella typhimurium (TA98, TA100, TA1535, and TA1537) and Escherichia coli (WP2uvrA) tester strains. Meanwhile, the results of the in vitro chromosomal aberration and in vivo micronucleus tests revealed that SUNACTIVE Zn-P240 did not induce chromosomal aberrations. These results suggest that SUNACTIVE Zn-P240 did not exhibit mutagenic or clastogenic properties in vitro and in vivo.

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Abbreviations

2-AA:

2-Aminoanthracene

9-AA:

9-Aminoacridine hydrochloride monohydrate

AF-2:

2-(2-Furyl)-3-(5-nitro-2-furyl)acrylamide

CHL:

Chinese hamster lung

CPA:

Cyclophosphamide monohydrate

FBS:

Fetal bovine serum

MNPCE:

Micronucleated polychromatic erythrocyte

NaN3 :

Sodium azide

NCE:

Normochromatic erythrocyte

OECD:

Organisation for Economic Cooperation and Development

PCE:

Polychromatic erythrocyte

RICC:

Relative increase in cell count

SEM:

Scanning electron microscopy

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Funding

This study was financially supported by Chonnam National University (Grant number: 2020-1891). This work was also supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (NRF- 2021R1A2C2011673).

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Authors and Affiliations

  1. National Institute of Agricultural Sciences, Wanju, Jeonbuk, 55365, Republic of Korea

    Jeong-Hyun Lim

  2. College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, 61186, Republic of Korea

    Jeong-Hyun Lim, Woong-Il Kim, So-Won Pak, Se-Jin Lee, In-Sik Shin & Jong-Choon Kim

  3. Health Care Institute, Korea Testing and Research Institute, Hwasun, Jeonnam, 58141, Republic of Korea

    Jong-Yun Lee

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  1. Jeong-Hyun Lim
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Lim, JH., Lee, JY., Kim, WI. et al. Evaluation of genotoxicity of SUNACTIVE Zn-P240 in vitro and in vivo. Toxicol Res. 38, 459–467 (2022). https://doi.org/10.1007/s43188-022-00128-2

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