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Obtaining antioxidant compounds from the endophytic fungus Diaporthe schini using heat- and ultrasound-assisted extraction

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Abstract

Ultrasound-assisted extraction (UAE) of antioxidant compounds from Diaporthe schini biomass using ethanol as solvent was performed and the results were compared with heat-assisted extraction (HAE; conventional technique). Ultrasound intensity (17–85 W cm−2) and pulse cycle (0.50–1.00) were evaluated according to a Central Composite Rotatable Design (CCRD) to obtain the highest extraction yield. The HAE was studied at 30 and 50 °C. In the validation condition (85 W cm−2 and 0.93) of the UAE the extraction yield and antioxidant capacity (DPPH) were 22.30 ± 0.47% and 91.35 ± 0.27%, respectively. The best extraction yield (8.34 ± 0.38%) and antioxidant activity (91.32 ± 0.98%) of the HAE were observed at 50 ºC. The GC/MS analysis of the UAE extracts showed the presence of methyl 9,12 octadecadienoate (Z, Z)-, ethyl ester of hexadecanoic acid, ethyl octadec-9-enoate, and 1,4-diaza-2,5-dioxo-3-isobutyl bicyclo[4.3.0]nonane, which could be responsible for the antioxidant activity observed. This study was the first attempt to optimize the UAE parameters for the antioxidant compounds extraction from Diaporthe schini. UAE improved the extraction yield and reduced the extraction time in relation to HAE.

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Acknowledgements

The authors would like to thank CAPES (Coordination for the Improvement of Higher Education Personnel)–Finance Code 001, CNPq (National Council for Scientific and Technological Development and Research Support Foundation of the State of Rio Grande do Sul (FAPERGS) for the financial support.

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Authors and Affiliations

  1. Department of Chemical Engineering, Federal University of Santa Maria, 1000, Roraima avenue, Santa Maria, 97105-900, Brazil

    Barbara Vargas da Rosa, Gabriel da Silva Sauzem & Raquel Cristine Kuhn

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  1. Barbara Vargas da Rosa
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  2. Gabriel da Silva Sauzem
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  3. Raquel Cristine Kuhn
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Correspondence to Raquel Cristine Kuhn.

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da Rosa, B.V., Sauzem, G.d.S. & Kuhn, R.C. Obtaining antioxidant compounds from the endophytic fungus Diaporthe schini using heat- and ultrasound-assisted extraction. Braz. J. Chem. Eng. 38, 189–195 (2021). https://doi.org/10.1007/s43153-021-00089-3

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  • DOI: https://doi.org/10.1007/s43153-021-00089-3

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