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Effect of Five Different Antioxidants on the Effectiveness of Goat Semen Cryopreservation

  • Reproductive Biology: Original Article
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A Correction to this article was published on 07 February 2024

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Abstract

The effective combination of semen cryopreservation and artificial insemination has a positive effect on the conservation of germplasm resources, production and breeding, etc. However, during the process of semen cryopreservation, the sperm cells are very susceptible to different degrees of physical, chemical, and oxidative stress damage. Oxidative damage is the most important factor that reduces semen quality, which is affected by factors such as dilution equilibrium, change of osmotic pressure, cold shock, and enzyme action during the freezing–thawing process, which results in the aggregation of a large amount of reactive oxygen species (ROS) in sperm cells and affects the quality of semen after thawing. Therefore, the method of adding antioxidants to semen cryoprotective diluent is usually used to improve the effect of semen cryopreservation. The aim of this experiment was to investigate the effects of adding five antioxidants (GLP, Mito Q, NAC, SLS, and SDS) to semen cryoprotection diluent on the cryopreservation effect of semen from Saanen dairy goats. The optimal preservation concentrations were screened by detecting sperm viability, plasma membrane integrity, antioxidant capacity, and acrosomal enzyme activities after thawing, and the experimental results were as follows: the optimal concentrations of GLP, Mito Q, NAC, SLS, and SDS added to semen cryopreservation diluent at different concentrations were 0.8 mg/mL, 150 nmol/L, 0.6 mg/mL, 0.15 mg/ mL, 0.6 mg/mL, and 0.15 mg/mL. The optimal concentrations of the five antioxidants were added to the diluent and analyzed after 1 week of cryopreservation, and it was found that sperm viability, plasma membrane integrity, and mitochondrial activity were significantly enhanced after thawing compared with the control group (P < 0.05), and their antioxidant capacity was significantly enhanced (P < 0.05). Therefore, the addition of the above five antioxidants to goat sperm cryodilution solution had a better enhancement of sperm cryopreservation. This study provides a useful reference for exploring the improvement of goat semen cryoprotection effect.

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Acknowledgements

All of the authors would like to express their gratitude and extend their appreciation to their respective institutes and universities.

Funding

This study was supported by the Shaanxi Provincial Key Research and Development Program (No. 2018ZDXM-NY-040).

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Authors and Affiliations

  1. College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, People’s Republic of China

    Xiaohua Yi, Yanbo Qiu, Xiaoqin Tang, Yichen Lei, Yun Pan, Sayed Haidar Abbas Raza, Yu Zhang & Xiuzhu Sun

  2. Guangdong Provincial Key Laboratory of Food Quality and Safety/Nation-Local Joint Engineering Research Center for Machining and Safety of Livestock and Poultry Products, South China Agricultural University, Guangzhou, 510642, China

    Sayed Haidar Abbas Raza

  3. Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region, Shaoguan University, Shaoguan, 512005, China

    Sayed Haidar Abbas Raza

  4. Biology Department, College of Science and Humanities, Shaqra University, Al Quwaiiyah, 19257, Al Quwaiiyah, Saudi Arabia

    Norah A. Althobaiti

  5. Faculty of Science, Department of Biology, University of Tabuk, 47913, Tabuk, Saudi Arabia

    Aishah E. Albalawi

  6. Department of Pathology, College of Medicine, Qassim University, P.O. Box 6655, Buraidah, 51452, Kingdom of Saudi Arabia

    Waleed Al Abdulmonem

  7. Department of Parasitology, Faculty of Medicine, Umm Al Qura University, P.O. Box 715, 21955, Makkah, Saudi Arabia

    Raafat T. M. Makhlof

  8. College of Medicine, Umm AL Qura University, 21955, Makkah, Saudi Arabia

    Mohammad A. Alsaad

  9. College of Grassland Agriculture, Northwest A&F University, Yangling, China

    Xiuzhu Sun

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  13. Xiuzhu Sun
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Contributions

Xiaohua Yi, Yanbo Qiu, Xiaoqin Tang: conceptualization; data curation; formal analysis; investigation; methodology; software; validation; writing—original draft; writing—review and editing.Yichen Lei, Yun Pan, Sayed Haidar Abbas Raza: conceptualization; data curation; formal analysis; investigation; methodology; software; validation; review and editing. Norah A. Althobaiti, Aishah E. Albalawi, Waleed Al Abdulmonem, Raafat T. M. Makhlof, Mohammad A. Alsaad: formal analysis; writing—review and editing software; software; methodology; visualization. Yu Zhang: writing—review and editing; software; methodology. Xiuzhu Sun: conceptualization; funding acquisition; project administration; resources; supervision; visualization; writing—review and editing. All authors have read and agreed to the published version of the manuscript.

Corresponding author

Correspondence to Xiuzhu Sun.

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The Committee approved the procedures for animal handling for experiments of Experimental Animal Management at Northwest A&F University, China (protocol number: NWAFU2022-019). Moreover, all applicable rules and regulation of the organization and government were followed regarding the ethical use of experimental animals.

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The authors declare no competing interests.

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Yi, X., Qiu, Y., Tang, X. et al. Effect of Five Different Antioxidants on the Effectiveness of Goat Semen Cryopreservation. Reprod. Sci. (2024). https://doi.org/10.1007/s43032-024-01452-8

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