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Uroplakin 1a Interacts with Regucalcin and Proteasome Subunit Beta 1

  • Male Reproduction: Original Article
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Abstract

Uroplakins (UPKs) are specialized proteins that plan an important role in protecting the epithelium of the bladder from toxic waste. We recently demonstrated the expression pattern of UPKs in the male reproductive tract and their importance in sperm function in murine models. However, the exact mechanisms through which UPKs affect spermatogenesis are not reported. In this study, using yeast two-hybrid screening was conducted to determine the interaction partners of Uroplakin 1a (UPK1A). Y2H Gold yeast strain overexpressing UPK1A was mated with Y187 yeast strain overexpressing human testis cDNA library and the mutants were plated on SD agar plates containing selection media. Colonies that grew on SD/-Trp, SD/-Leu, SD/-His, and SD/-Ade plates were isolated and evaluated to identify the interacting partners of UPK1A. Regucalcin (RGN) and proteasome subunit beta 1 (PSMB1) were identified as potential interaction partners. Using HEK cells that overexpress UPK1A and RGN or PMSB1, the co-localization and interaction were estimated with high-resolution microscopy and Pearson’s coefficient. In light of the fact that UPK1A knockout caused subfertility and that the role of RGN and PSMB1 in spermatogenesis is documented, an interaction between UPK1A and RGN or PSMB1 could be required for spermatogenesis.

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Acknowledgements

SBM received junior and senior research fellowship from Government of India. We thank the facilities extended by UGC-SAP, UGC-CAS, DBT-CREBB, DST-PURSE, UGC-UPE-II, FIST, and IoE programmes at School of Life Sciences, University of Hyderabad. JA received the junior and senior research fellowship from Department of Biotechnology, Government of India.

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  1. Suresh Babu Munipalli

    Present address: National Institute of Child Health and Human Development, Rockville, MD, 20847, USA

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  1. Department of Animal Biology, University of Hyderabad, Hyderabad, 500046, India

    Suresh Yenugu

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SBM carried out the experimental work. SY conceptualized the ideas, wrote the manuscript, and provided the reagents and other facilities.

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Correspondence to Suresh Yenugu.

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Munipalli, S.B., Yenugu, S. Uroplakin 1a Interacts with Regucalcin and Proteasome Subunit Beta 1. Reprod. Sci. 30, 3520–3528 (2023). https://doi.org/10.1007/s43032-023-01292-y

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