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Association Between Functional Activity of Mitochondria and Actin Cytoskeleton Instability in Oocytes from Advanced Age Mice

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Abstract

Mitochondrial dysfunction is strongly associated with the oocyte quality and aging, wherein the aged oocytes are related to the actin cytoskeleton integrity; however, whether this integrity is associated with mitochondrial dysfunction in oocytes from aged mice remains unclear. In the present study, we investigated the relationship between mitochondrial dysfunction and actin cytoskeleton instability in oocytes from the aged mice. We performed comparable analysis of mitochondrial motility between young, 1.5 μM cytochalasin B (CB)-treated young oocytes, and aged oocytes by confocal live imaging. Moreover, we analyzed the relationships between mitochondrial motility and maturation ratios, including ATP production ratio of the young, CB-treated young, and aged oocytes. Actin cytoskeleton instability in the aged oocytes and CB-treated young oocytes led to a significant decrease in the mitochondrial motility and low ATP productive ratios compared to those in the young group. Our data suggest that the actin cytoskeleton instability is presumably the primary cause for the loss of mitochondrial function in the aged murine oocytes.

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Funding

This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) [grant numbers 2018R1D1A1B07050138, 2018R1C1B5045516, 2018R1D1A1B07044016 and 2019R1A2C1086882].

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Author notes

  1. Myung Joo Kim and Kyoung Hee Choi contributed equally to this work.

Authors and Affiliations

  1. CHA Fertility Center Seoul Station, CHA University School of Medicine, Seoul, 04637, South Korea

    Myung Joo Kim, Kyoung Hee Choi, Dong Woo Seo, Hye Ran Lee & Jae Ho Lee

  2. Department of Animal Biotechnology, Konkuk University, Seoul, 05029, South Korea

    Kyoung Hee Choi & Hoon Taek Lee

  3. Department of Animal Biotechnology & Resource, Sahmyook University, Seoul, 01795, South Korea

    Hyun Seok Kong, Chan Hee Lee & Sang Jin Lee

  4. Department of Obstetrics and Gynecology, Fertility Center of CHA Gangnam Medical Center, CHA University School of Medicine, Seoul, 06135, South Korea

    Woo Sik Lee

  5. Department of Biomedical Science, College of Life Science, CHA University, Pocheon, 11160, South Korea

    Jung Jae Ko & Jae Ho Lee

  6. Department of Obstetrics and Gynecology, Fertility Center of CHA Bundang Medical Center, CHA University School of Medicine, Seongnam, 13496, South Korea

    Ji Hyang Kim

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  1. Myung Joo Kim
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Correspondence to Ji Hyang Kim or Jae Ho Lee.

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Graph of mitochondrial location ratios in the nuclear and cytoplasm (cyto) area in the ooplasm of young and aged oocytes from the GVBD to MII phase (cytoplasm and near nuclear area) (JPG 26 kb)

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Kim, M.J., Choi, K.H., Seo, D.W. et al. Association Between Functional Activity of Mitochondria and Actin Cytoskeleton Instability in Oocytes from Advanced Age Mice. Reprod. Sci. 27, 1037–1046 (2020). https://doi.org/10.1007/s43032-020-00145-2

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  • DOI: https://doi.org/10.1007/s43032-020-00145-2

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