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The effects of bee venom on liver and skeletal muscle in exhaustive swimming rats

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Abstract

Oxidative damage and proinflammatory cytokines are involved in exhaustive exercise-induced fatigue. This study aimed to investigate the effects of bee venom, a natural toxin, on fatigue and tissue damage in rats that underwent forced swimming exercise. Rats were divided into four groups: control, swimming exercise (SE), bee venom (BV) and swimming exercise + bee venom (SE + BV). SE and SE + BV groups were subjected to forced swimming (load of 7% body weight) for 5 days. BV and SE + BV groups were injected with 1 mg/kg BV subcutaneously. Swimming time, blood lactate and TNF-α levels, MDA and GSH levels in liver and gastrocnemius muscle were evaluated. Swimming time was shorter in SE + BV group than SE group. There was no difference in lactate levels between SE and SE + BV groups. MDA and GSH levels were increased in SE, BV and SE + BV groups. TNF-α levels were increased in BV group compared to control and SE groups. Our study demonstrated that BV administration before exhaustive exercise in rats did not provide anti-fatigue effect. Additionally, BV did not show anti-inflammatory activity and had different effects on antioxidant capacity at tissue level. Further research might explore the effects of different doses and durations of BV on exhaustive exercise.

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References

  • Abd El-Haleim EA (2020) Molecular study on the potential protective effects of bee venom against fructose-induced nonalcoholic steatohepatitis in rats. Pharmacology 105(11–12):692–704

    Article  CAS  Google Scholar 

  • Açıkel Elmas M, Atay N, Bingöl Özakpınar Ö, Arbak S, Kolgazi M, Şener G, Ercan F (2020) Morphological evaluation of the effects of exercise on high-fat-diet-induced liver damage in rats. Turk J Gastroenterol 31(9):626–632

    Article  Google Scholar 

  • Ahmed O, Fahim H, Mahmoud A, Eman Ahmed EA (2017) Bee venom and hesperidin effectively mitigate complete freund’s adjuvant-induced arthritis via immunomodulation and enhancement of antioxidant defense system. Arch Rheumatol 33(2):198–212

    Article  Google Scholar 

  • Akil M, Gurbuz U, Bicer M, Halifeoglu I, Baltaci AK, Mogulkoc R (2015) Selenium prevents lipid peroxidation in liver and lung tissues of rats in acute swimming exercise. BratislLekListy 116(4):233–235. https://doi.org/10.4149/bll_2015_045 (PMID: 25773950)

    Article  CAS  Google Scholar 

  • Altinoz E, Ozmen T, Oner Z, Elbe H, Erdemli ME, Bag HG (2016) Saffron (its active constituent, crocin) supplementation attenuates lipid peroxidation and protects against tissue injury. BratislLekListy 117(7):381–387. https://doi.org/10.4149/bll_2016_075 (PMID: 27546539)

    Article  CAS  Google Scholar 

  • An HJ, Kim JY, Kim WH, Gwon MG, Gu HM, Jeon MJ, Han SM, Pak SC, Lee CK, Park IS, Park KK (2018) Therapeutic effects of beevenom and its major component, melittin, on atopic dermatitis in vivo and in vitro. Br J Pharmacol 175(23):4310–4324

    Article  CAS  Google Scholar 

  • Casuso RA, Aragon-Vela J, Huertas JR, Ruiz-Ariza A, Martínez-Lopez EJ (2018) Comparison of the inflammatory and stress response between sprint interval swimming and running. Scand J Med Sci Sports 28(4):1371–1378

    Article  CAS  Google Scholar 

  • Chang Q, Miao X, Ju X, Zhu L, Huang C, Huang T, Zuo X, Gao C (2013) Effects of pulse current on endurance exercise and its anti-fatigue properties in the hepatic tissue of trained rats. PLoS One 8(10):e75093

    Article  CAS  Google Scholar 

  • Chang CC, Chen CW, Owaga E, Lee WT, Liu TN, Hsieh RH (2020) Mangosteen concentrate drink supplementation promotes antioxidant status and lactate clearance in rats after exercise. Nutrients 12(5):1447

    Article  CAS  Google Scholar 

  • El-Aarag B, Magdy M, AlAjmi MF, Khalifa SAM, El-Seedi HR (2019) Melittin exerts beneficial effects on paraquat-induced lung injuries in mice by modifying oxidative stress and apoptosis. Molecules 24(8):1498. https://doi.org/10.3390/molecules24081498.PMID:30995821;PMCID:PMC6514788

    Article  CAS  PubMed Central  Google Scholar 

  • Gajski G, Garaj-Vrhovac V (2011) Bee venom induced cytogenetic damage and decreased cell viability in human white blood cells after treatment in vitro: a multi-biomarker approach. Environ Toxicol Pharmacol 32(2):201–211

    Article  CAS  Google Scholar 

  • Gajski G, Garaj-Vrhovac V (2013) Melittin: a lytic peptide with anticancer properties. Environ Toxicol Pharmacol 36(2):697–705

    Article  CAS  Google Scholar 

  • Gajski G, Domijan AM, Garaj-Vrhovac V (2012) Alterations of GSH and MDA levels and their association with bee venom-induced DNA damage in human peripheral blood leukocytes. Environ Mol Mutagen 53(6):469–477. https://doi.org/10.1002/em.21708 (Epub 2012 Jun 25 PMID: 22730252)

    Article  CAS  PubMed  Google Scholar 

  • Gajski G, Domijan AM, Žegura B, Štern A, Gerić M, Novak Jovanović I, Vrhovac I, Madunić J, Breljak D, Filipič M, Garaj-Vrhovac V (2016) Melittin induced cytogenetic damage, oxidative stress and changes in gene expression in human peripheral blood lymphocytes. Toxicon 110:56–67

    Article  CAS  Google Scholar 

  • Garaj-Vrhovac V, Gajski G (2009) Evaluation of the cytogenetic status of human lymphocytes after exposure to a high concentration of bee venom in vitro. Arh Hig Rada Toksikol 60(1):27–34

    Article  Google Scholar 

  • Gu H, Kim WH, An HJ, Kim JY, Gwon MG, Han SM, Leem J, Park KK (2018) Therapeutic effects of bee venom on experimental atopic dermatitis. Mol Med Rep 18(4):3711–3718

    CAS  PubMed  PubMed Central  Google Scholar 

  • Hanafi MY, Zaher ELM, El-Adely SEM, Sakr A, Ghobashi AHM, Hemly MH, Kazem AH, Kamel MA (2018) The therapeutic effects of bee venom on some metabolic and antioxidant parameters associated with HFD-induced non-alcoholic fatty liver in rats. ExpTher Med 15(6):5091–5099

    Google Scholar 

  • Hellner M, Winter D, von Georgi R, Münstedt K (2008) Apitherapy: usage and experience in german beekeepers. Evid Based Compl Alternat Med 5(4):475–479

    Article  Google Scholar 

  • Kocyigit A, Guler EM, Kaleli S (2019) Anti-inflammatory and antioxidative properties of honey bee venom on Freund’s Complete Adjuvant-induced arthritis model in rats. Toxicon 161:4–11

    Article  CAS  Google Scholar 

  • Koshinaka K, Honda A, Masuda H, Sato A (2020) Effect of quercetin treatment on mitochondrial biogenesis and exercise-induced amp-activated protein kinase activation in rat skeletal muscle. Nutrients 12(3):729

    Article  CAS  Google Scholar 

  • Li TG, Shui L, Ge DY, Pu R, Bai SM, Lu J, Chen YS (2019) Moxibustion reduces inflammatory response in the hippocampus of a chronic exercise-induced fatigue rat. Front IntegrNeurosci 20(13):48. https://doi.org/10.3389/fnint.2019.00048.PMID:31616260;PMCID:PMC6763602

    Article  Google Scholar 

  • Lima FD, Stamm DN, Della-Pace ID, Dobrachinski F, de Carvalho NR, Royes LFF, Soares FA, Rocha JB, González-Gallego J, Bresciani G (2013) Swimming training induces liver mitochondrial adaptations to oxidative stress in rats submitted to repeated exhaustive swimming bouts. PLoS One 8(2):e55668. https://doi.org/10.1371/journal.pone.0055668

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lin TC, Wang SH, Huang CC, Lai YC, Song TY, Tsai MS (2018) Anti-fatigue, antioxidation and anti-inflammatory effects of eucalyptus oil aromatherapy in swimming-exercised rats. Chin J Physiol 61(5):257–265

    Article  CAS  Google Scholar 

  • Liu L, Wu X, Zhang B, Yang W, Li D, Dong Y, Yin Y, Chen Q (2017) Protective effects of tea polyphenols on exhaustive exercise-induced fatigue, inflammation and tissue damage. Food Nutr Res 61(1):1333390

    Article  Google Scholar 

  • Meligi NM, Ismail SA, Tawfik NS (2020) Protective effects of honey and beevenom against lipopolysaccharide and carbon tetrachloride-induced hepatoxicity and lipid peroxidation in rats. Toxicol Res (Camb) 9(5):693–705

    Article  Google Scholar 

  • Oršolić N (2012) Bee venom in cancer therapy. Cancer Metastasis Rev 31(1–2):173–194

    Article  Google Scholar 

  • Pala R, Genc E, Tuzcu M, Orhan C, Sahin N, Er B, Cinar V, Sahin K (2018) L-Carnitine supplementation increases expression of PPAR-γ and glucose transporters in skeletal muscle of chronically and acutely exercised rats. Cell Mol Biol 64(1):1–6

    Article  Google Scholar 

  • Shin IS, Kim DH, Jang EY, Kim HY, Yoo HS (2019) Anti-fatigue properties of cultivated wild ginseng distilled extract and its active component panaxydol in rats. J Pharm 22(2):68–74

    Google Scholar 

  • Shui L, Yi RN, Wu YJ, Bai SM, Si Q, Bo AG, Wuyun GR, Si LG, Chen YS, Lu J (2020) Effects of mongolian warm acupuncture on iNOS/NO and inflammatory cytokines in the hippocampus of chronic fatigue rats. Front IntegrNeurosci 31(13):78. https://doi.org/10.3389/fnint.2019.00078.PMID:32082125;PMCID:PMC7006054

    Article  Google Scholar 

  • Son DJ, Lee JW, Lee YH, Song HS, Lee CK, Hong JT (2007) Therapeutic application of anti-arthritis, pain-releasing, and anti-cancer effects of bee venom and its constituent compounds. Pharmacol Ther 115(2):246–270

    Article  CAS  Google Scholar 

  • Steckling FM, Lima FD, Farinha JB, Rosa PC, Royes LFF, Cuevas MJ, Bresciani G, Soares FA, González-Gallego J, Barcelos RP (2020) Diclofenac attenuates inflammation through TLR4 pathway and improves exercise performance after exhaustive swimming. Scand J Med Sci Sports 30(2):264–271

    Article  Google Scholar 

  • Tekeoğlu İ, Akdoğan M, Çelik İ (2020) Investigation of anti-inflammatory effects of bee venom in experimentally induced adjuvant arthritis. Reumatologia 58(5):265–271

    Article  Google Scholar 

  • Xu J, Li Y (2012) Effects of salidroside on exhaustive exercise-induced oxidative stress in rats. Mol Med Rep 6(5):1195–1198. https://doi.org/10.3892/mmr.2012.1060 (Epub 2012 Sep 3 PMID: 22948446)

    Article  CAS  PubMed  Google Scholar 

  • Yang DK, Lee SJ, Adam GO, Kim SJ (2020) Aralia continentalis kitagawa extract attenuates the fatigue induced by exhaustive exercise through inhibition of oxidative stress. Antioxidants (Basel) 9(5):379

    Article  CAS  Google Scholar 

  • Yoon SY, Roh DH, Kwon YB, Kim HW, Seo HS, Han HJ, Lee HJ, Beitz AJ, Lee JH (2009) Acupoint stimulation with diluted bee venom (apipuncture) potentiates the analgesic effect of intrathecal clonidine in the rodent formalin test and in a neuropathic pain model. J Pain 10(3):253–263

    Article  CAS  Google Scholar 

  • Zhang S, Liu Y, Ye Y, Wang XR, Lin LT, Xiao LY, Zhou P, Shi GX, Liu CZ (2018) Bee venom therapy: potential mechanisms and therapeutic applications. Toxicon 148:64–73

    Article  CAS  Google Scholar 

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Funding

No funding was received for conducting this study.

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Authors and Affiliations

  1. Department of Physiology, Medical Faculty, Dokuz Eylul University, Balcova, Izmir, 35330, Turkey

    Askin Senturk, Bahar Dalkiran, Burcu Acikgoz, Ilkay Aksu, Osman Acikgoz & Muge Kiray

  2. Graduate School of Health Sciences, Dokuz Eylul University, Balcova, Izmir, 35330, Turkey

    Askin Senturk, Bahar Dalkiran & Burcu Acikgoz

Authors
  1. Askin Senturk
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  2. Bahar Dalkiran
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  3. Burcu Acikgoz
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  4. Ilkay Aksu
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  5. Osman Acikgoz
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  6. Muge Kiray
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Contributions

AS and MK designed the study. AS, BD and BA performed the in vivo study. MK, BD and BA performed the biochemical analysis. IA and OA analyzed the data. MK, IA and OA wrote the manuscript.

Corresponding author

Correspondence to Muge Kiray.

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The authors have no conflict of interest.

Ethical approval

All experiments were performed in accordance with the guidelines provided by the Experimental Animal Laboratory and approved by the Ethics Committee of the Dokuz Eylul University Medical Faculty, Turkey (approval number: 43/2020). All experimental procedures were appropriate to the Guide for the Care and Use of Laboratory Animals of National Institutes of Health (USA).

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Senturk, A., Dalkiran, B., Acikgoz, B. et al. The effects of bee venom on liver and skeletal muscle in exhaustive swimming rats. BIOLOGIA FUTURA 73, 237–244 (2022). https://doi.org/10.1007/s42977-022-00115-6

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  • DOI: https://doi.org/10.1007/s42977-022-00115-6

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