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An electrochemical sensor based on graphene-chitosan-cyclodextrin modification for the detection of Staphylococcus aureus

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Abstract

This study reports the synthesis of a novel graphene/chitosan/β-cyclodextrin composite material (GO/CS/β-CD) via a one-step chemical reduction method, which combines the advantages of graphene, chitosan, and β-cyclodextrin. The morphology and structure of the composite were characterized using various techniques, such as scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and Raman spectroscopy. Subsequently, sortase A (SA) was immobilized onto the GO/CS/β-CD for the detection of Staphylococcus aureus. The sensor exhibited a good linear relationship within the concentration range of 30–300 CFU/mL, with a detection limit of 12 CFU/mL. The GO/CS/β-CD composite material showed enhanced properties due to the synergistic effect of graphene, chitosan, and β-cyclodextrin. The immobilization of sortase A onto the composite material improved the sensitivity and selectivity of the sensor for the detection of S. aureus. This study presents a novel graphene/chitosan/β-cyclodextrin composite material with immobilized sortase A, demonstrating enhanced sensitivity and selectivity for the detection of Staphylococcus aureus, which has potential for the development of high-performance sensors in various fields.

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The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Authors and Affiliations

  1. Department of Infectious Diseases, Wangcang People’s Hospital, Wangcang, 628200, Guangyuan, People’s Republic of China

    Guochun Li

  2. Department of Cardiovascular Medicine, Wangcang Hospital of Traditional Chinese Medicine, Wangcang, 628200, Guangyuan, People’s Republic of China

    Jiaxin Li

  3. Department of Respiratory and Critical Care Medicine, Wangcang County People’s Hospital, Wangcang, 628200, Guangyuan, People’s Republic of China

    Ziyu Yang

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  1. Guochun Li
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Contributions

Conceptualization, GL; software, JL; validation, GL, and ZY; formal analysis, GL; investigation, GL, and JL; resources, ZY; data curation, GL, JL, and ZY; writing—original draft preparation, GL; writing—review and editing, JL and ZY; visualization, GL; supervision, ZY; project administration, ZY All authors have read and agreed to the published version of the manuscript.

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Correspondence to Guochun Li.

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Li, G., Li, J. & Yang, Z. An electrochemical sensor based on graphene-chitosan-cyclodextrin modification for the detection of Staphylococcus aureus. Carbon Lett. 34, 495–504 (2024). https://doi.org/10.1007/s42823-023-00518-y

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