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Optimization studies on laccase activity of Proteus mirabilis isolated from treatment sludge of textile industry factories

Optimization of laccase activity of Proteus mirabilis

  • Biotechnology and Industrial Microbiology - Research Paper
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Abstract

Laccase is an exothermic enzyme with copper in its structure and has an important role in biodegradation by providing oxidation of phenolic compounds and aromatic amines and decomposing lignin. The aim of this study is to reach maximum laccase enzyme activity with minimum cost and energy through optimization studies of Proteusmirabilis isolated from treatment sludge of a textile factory. In order to increase the laccase enzyme activities of the isolates, medium and culture conditions were optimized with the study of carbon (Glucose, Fructose, Sodium Acetate, Carboxymethylcellulose, Xylose) and nitrogen sources (Potassium nitrate, Yeast Extract, Peptone From Soybean, Bacteriological Peptone), incubation time, pH, temperature and Copper(II) sulfate concentration then according to the results obtained. Response Surface Method (RSM) was performed on six different variables with three level. According to the data obtained from the RSM, the maximum laccase enzyme activity is reached at pH 7.77, temperature 30.03oC, 0.5 g/L CuSO4, 0.5 g/L fructose and 0.082 g/L yeast extract conditions. After all, the laccase activity increased 2.7 times. As a result, laccase activity of P. mirabilis can be increased by optimization studies. The information obtained as a result of the literature studies is that the laccase enzymes produced in laboratory and industrial scale are costly and their amounts are low. This study is important in terms of obtaining more laccase activity from P.mirabilis with less cost and energy.

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References

  1. Robinson PK (2015) Enzymes: principles and biotechnological applications. Essays Biochem 59:1. https://doi.org/10.1042/bse0590001

    Article  PubMed  PubMed Central  Google Scholar 

  2. Çevik A (2017) Bacillus sp. determination of optimum ph and temperature values of the laccase enzyme that have been recognized. Dissertation, University of Kahramanmaraş Sütçü Imam

  3. Altıntaş B Removal of textile dyes from artificial waste water using enyzme laccase. Dissertation, Gazi University [4], Rodriguez S, (2018) Solid-state fermentation for laccases production and their applications, In Current Developments in Biotechnology and Bioengineering, Elsevier: 211–234., Sarabia LA, Ortiz MC, Sanchez MS (2011) (2020) Response Surface Methodology: 287–326

  4. Bozoğlu C (2014) Purification of laccase enzyme of thermophilic Brevibacillus sp. isolated from Agri Diyadin hot spring and determination of potential industrial usage of the enzyme. Dissertation, University of Atatürk

  5. Rezaei S, Shahverdi AR, Faramarzi MA (2017) Isolation, one-step affinity purification, and characterization of a polyextremotolerant laccase from the halophilic bacterium Aquisalibacillus elongatus and its application in the delignification of sugar beet pulp. Bioresour Technol 67–75. https://doi.org/10.1016/j.biortech.2017.01.036

  6. Olukanni OD, Osuntoki AA et al (2010) l Decolorization and biodegradation of Reactive Blue 13 by Proteus mirabilis LAG. Journal of Hazardous Materials, 184(1–3), 290–298. https://doi.org/10.1016/j.jhazmat.2010.08.035

  7. Coker C, Poore CA, Li X, Mobley HL (2000) Pathogenesis of Proteus mirabilisurinary tract infection. Microbes Infect 2(12):1497–1505. https://doi.org/10.1016/S1286-4579(00)01304-6

    Article  CAS  PubMed  Google Scholar 

  8. Akyıl MH (2021) Investigation of contact-dependent inhibition (CDI) mechanism in some gram negative bacteria. Dissertation, University of Hacettepe

  9. Dean A, Voss D (eds) (1999) Design and analysis of experiments. Springer New York, New York, NY, pp 483–545

    Book  Google Scholar 

  10. Gökçe B, Taşgetiren S (2009) Experimental Design for Quality. Electron Magazine Mach Technol 6(1):71–83

    Google Scholar 

  11. Khuri AI, Mukhopadhyay S (2010) Response surface methodology. Wiley Interdisciplinary Reviews: Comput Stat 2(2):128–149. https://doi.org/10.1002/wics.73

    Article  Google Scholar 

  12. Oztat K, Filik Iscen C (2022) Laccase enzyme activities of bacteria isolated from paper and textile industry factories treatment sludge. International Congress: 4. International Palandoken Scientific Studies Congress, April 2022: 406–413

  13. Telke AA, Ghodake GS, Dhanve RS, Govindwar SP, Kalyani DC (2011) Biochemical characteristics of a textile dye degrading extracellular laccase from a Bacillus sp. ADR Bioresource Technol 1752–1756. https://doi.org/10.1016/j.biortech.2010.08.086

  14. Kesebir A (2020) Purification of laccase enzyme from b. licheniformis o12 bacteria, recombinant production from bacteria dna, characterization, biotechnological applications. Dissertation, University of Atatürk

  15. Kaushik G, Thakur IS (2014) Production of laccase and optimization of its production by Bacillus sp. using distillery spent wash as inducer. Bioremediat J 18(1):28–37. https://doi.org/10.1080/10889868.2013.834869

    Article  CAS  Google Scholar 

  16. Senthivelan T, Kanagaraj J, Panda RC, Narayani T (2019) Screening and production of a potential extracellular fungal laccase from Penicillium Chrysogenum: media optimization by response surface methodology (RSM) and central composite rotatable design (CCRD). Biotechnol Rep 23:e00344. https://doi.org/10.1016/j.btre.2019.e00344

  17. Kuddus M, Joseph B, Ramteke PW (2013) Production of laccase from newly isolated Pseudomonas putida and its application in bioremediation of synthetic dyes and industrial effluents. Biocatal Agric Biotechnol 2(4):333–338. https://doi.org/10.1016/j.bcab.2013.06.002

  18. Bergey DH (1994) Bergey’s manual of determinative bacteriology. Lippincott Williams & Wilkins.Maryland, USA

  19. Barredo JL (2005) Microbial enzymes and Biotransformations. Humana Pres, Totowa, New Jersey

    Book  Google Scholar 

  20. Hernández-Monjaraz WS, Caudillo-Pérez C, Salazar-Sánchez PU, Macías-Sánchez KL (2018) Influence of iron and copper on the activity of laccases in Fusarium oxysporum f. sp. lycopersici. Brazilian J Microbiol 49:269–275. https://doi.org/10.1016/j.bjm.2018.06.002

    Article  CAS  Google Scholar 

  21. Oğün E, Özdemir Kl et al (2018) Characterization of the extracellular laccase-producing Bacillus megaterium s1 isolate isolated from Pinus sylvestris l forest soil in Sarıkamış (KARS) region. Yüzüncü Yıl Univ J Inst Sci Technol 23(1):79–86

    Google Scholar 

  22. Deepa, T., Gangwane, A. K., Sayyed, R. Z., Jadhav, H. P., & Mehjabeen. (2020). Optimization and scale-up of laccase production by Bacillus sp. BAB-4151 isolated from the waste of the soap industry. Environmental Sustainability, 3(4), 471–479.https://doi.org/10.1007/s42398-020-00135-9

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Acknowledgements

This work has been supported by Eskisehir Osmangazi University Scientific Research Projects Coordination Unit under grant number FYL_2021–2210. This study was produced from Kübra Oztat's master's thesis titled'Optimization studies on laccase activities of bacteria isolated from paper and textile industry factories treatment sludge' which was carried out under the supervision of Prof. Dr. Cansu Filik Işçen.

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Authors and Affiliations

  1. Eskisehir Osmangazi University, Eskişehir, Türkiye

    Kübra Oztat, Arzu Altın Yavuz & Cansu Filik Işçen

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  1. Kübra Oztat
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  2. Arzu Altın Yavuz
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  3. Cansu Filik Işçen
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Contributions

Oztat, Kübra: Formal analysis, investigation, methodology, project administration, resources, software, validation, writing– original draft, writing– review & editing.

Altın Yavuz, Arzu: Formal analysis, software, supervision, writing– review & editing.

Filik Işçen, Cansu: Methodology, project administration, supervision, writing– review & editing.

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Correspondence to Kübra Oztat or Cansu Filik Işçen.

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Responsible Editor: Eleni Gomes.

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Oztat, K., Yavuz, A. & Işçen, C. Optimization studies on laccase activity of Proteus mirabilis isolated from treatment sludge of textile industry factories. Braz J Microbiol 55, 1231–1241 (2024). https://doi.org/10.1007/s42770-024-01350-w

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  • DOI: https://doi.org/10.1007/s42770-024-01350-w

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