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Enterococcus mundtii A2 biofilm and its anti-adherence potential against pathogenic microorganisms on stainless steel 316L

  • Biotechnology and Industrial Microbiology - Research Paper
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Abstract

Pathogenic bacterial biofilms present significant challenges, particularly in food safety and material deterioration. Therefore, using Enterococcus mundtii A2, known for its antagonistic activity against pathogen adhesion, could serve as a novel strategy to reduce pathogenic colonization within the food sector. This study aimed to investigate the biofilm-forming ability of E. mundtii A2, isolated from camel milk, on two widely used stainless steels within the agri-food domain and to assess its anti-adhesive properties against various pathogens, especially on stainless steel 316L. Additionally, investigations into auto-aggregation and co-aggregation were also conducted. Plate count methodologies revealed increased biofilm formation by E. mundtii A2 on 316L, followed by 304L. Scanning electron microscopy (SEM) analysis revealed a dense yet thin biofilm layer, playing a critical role in reducing the adhesion of L. monocytogenes CECT 4032 and Staphylococcus aureus CECT 976, with a significant reduction of ≈ 2 Log CFU/cm2. However, Gram-negative strains, P. aeruginosa ATCC 27853 and E. coli ATCC 25922, exhibit modest adhesion reduction (~ 0.7 Log CFU/cm2). The findings demonstrate the potential of applying E. mundtii A2 biofilms as an effective strategy to reduce the adhesion and propagation of potentially pathogenic bacterial species on stainless steel 316L.

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Funding

The author Mariem Zanzan was the beneficiary of a scholarship from the National Center for Scientific and Technical Research (Grant number 18UIZ2016).

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Authors and Affiliations

  1. Bioprocess and Environment Team, LASIME Research Laboratory, Agadir Superior School of Technology, Ibn Zohr University, 33/S, 80150, Agadir, BP, Morocco

    Mariem Zanzan, Youssef Ezzaky & Fouad Achemchem

  2. Laboratory of Microbial Biotechnology and Vegetal Protection, Faculty of Sciences, Ibn Zohr University, Agadir, Morocco

    Mariem Zanzan & Fatima Hamadi

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  1. Mariem Zanzan
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Zanzan, M., Ezzaky, Y., Hamadi, F. et al. Enterococcus mundtii A2 biofilm and its anti-adherence potential against pathogenic microorganisms on stainless steel 316L. Braz J Microbiol (2024). https://doi.org/10.1007/s42770-024-01266-5

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