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Efficacy of calcium hypochlorite in disinfection of gutta-percha cones contaminated with Candida albicans

  • Clinical Microbiology - Research Paper
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Abstract

This study aimed to compare the efficacy of 2.5% sodium hypochlorite (NaOCl), 2.5% calcium hypochlorite [Ca(OCl)2], and 2% chlorhexidine (CHX) in the rapid disinfection of gutta-percha cones contaminated with Candida albicans. The minimum inhibitory and minimum fungicidal concentrations of each solution for C. albicans were determined and the ability of each solution to destroy and inhibit biofilm in culture wells was tested. In addition, ninety-eight gutta-percha cones contaminated with the fungal suspension were disinfected according to the type of solution (2.5% NaOCl, 2.5% Ca(OCl)2 or 2% CHX) in its different application methods (without agitation, ultrasonic agitation or agitation with Easy Clean), and regarding the exposure time to each irrigating solution (1 or 5 min). Next, the samples were checked for turbidity and evaluation of viable colonies. The compounds that showed the best performance in biofilm destruction were NaOCl and Ca(OCl)2 at a concentration of 2xMIC (p < 0.001). Regarding inhibited biofilm, the only compound that was effective at all MIC concentrations tested was 2.5% Ca(OCl)2 (p < 0.0001). Regarding the viable colonies, all solutions were effective concerning the control group, for all application methods, in 1 and 5 min (p < 0.05). The densitometer reading showed that CHX was the only effective solution in all application methods performed (p < 0.05). The results demonstrate that all tested solutions were effective in the rapid decontamination of cones contaminated with C. albicans.

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Funding

This study was partly funded by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) “Grant number 001”.

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Authors and Affiliations

  1. Post-Graduate Program in Oral Science, Faculty of Dentistry, Federal University of Santa Maria (UFSM), Roraima Avenue #1000, T Street, Building 26F, Room 2383, Santa Maria, Rio Grande Do Sul State, 97.105-900, Brazil

    Guilherme Pauletto & Carlos Alexandre Souza Bier

  2. Graduate Program in Dentistry, Faculty of Dentistry, Federal University of Santa Maria (UFSM), Santa Maria, Rio Grande Do Sul State, Brazil

    Pedro Henrique Fortes Guerim & Arthur Brites Barbosa

  3. Post-Graduate Program in Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Santa Maria (UFSM), Santa Maria, Rio Grande Do Sul State, Brazil

    Leonardo Quintana Soares Lopes

  4. Department of Microbiology and Parasitology, Faculty of Dentistry, Federal University of Santa Maria (UFSM), Santa Maria, Rio Grande Do Sul State, Brazil

    Patrícia Kolling Marquezan

Authors
  1. Guilherme Pauletto
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  2. Pedro Henrique Fortes Guerim
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Contributions

Guilherme Pauletto—Conceptualization; Data curation; Investigation; Methodology; Software; Validation; Visualization; Writing—original draft; Writing—review & editing.

Pedro Henrique Fortes Guerim—Data curation; Investigation; Methodology; Software; Validation; Visualization; Writing—review & editing.

Arthur Brites Barbosa—Data curation; Investigation; Methodology; Software; Validation; Visualization; Writing—review & editing.

Leonardo Quintana Soares Lopes – Data curation; Methodology; Software; Formal analysis; Validation; Writing—review & editing.

Carlos Alexandre Souza Bier—Conceptualization; Funding acquisition; Investigation; Methodology; Project administration; Resources; Software; Supervision; Validation; Visualization; Writing—review & editing.

Patrícia Kolling Marquezan—Conceptualization; Investigation; Methodology; Formal analysis; Project administration; Resources; Software; Supervision; Validation; Visualization; Writing—original draft; Writing—review & editing.

Corresponding author

Correspondence to Guilherme Pauletto.

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Pauletto, G., Guerim, P.H.F., Barbosa, A.B. et al. Efficacy of calcium hypochlorite in disinfection of gutta-percha cones contaminated with Candida albicans. Braz J Microbiol 55, 403–410 (2024). https://doi.org/10.1007/s42770-024-01255-8

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  • DOI: https://doi.org/10.1007/s42770-024-01255-8

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