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Ozonation for Pseudomonas paracarnis control: biofilm removal and preservation of chicken meat during refrigerated storage

  • Food Microbiology - Research Paper
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Abstract

Ozone has been studied to control microorganisms in food, as well as to control biofilm. In this context, the goals of this work were to determine the effect of ozonated water in the removal of Pseudomonas paracarnis biofilm and the effect of ozone gas and ozonated water on inactivating P. paracarnis in deboned chicken breast meat and its effect on product color. AISI 304 coupons were used as a surface for biofilm formation. The coupons were immerged into minimal medium for Pseudomonas inoculated with the P. paracarnis overnight culture (1% w/v) followed by incubation at 25 °C for 7 days. To obtain ozonized water, two different systems were used: system with microbubble generator (MB) and system with porous stone diffuser (PSD). The inlet ozone concentration was 19 mg/L and flow rate of 1 L/min. The coupons were subjected to ozonized water for 10 and 20 min. The chicken breast meat was exposed to gaseous ozone and ozonized water for 40 min. After the ozonation process, chicken meat samples were stored at 8 °C, for 5 days. More expressive removals of biofilm were obtained when using ozonized water obtained in the system with microbubble generator (MB for 20 min—reduction of 2.3 log cycles) and system with porous stone diffuser (PSD for 10 min—reduction of 2.7 log cycles; PSD for 20 min—reduction of 2.6 log cycles). The treatment of chicken meat with ozone gas resulted in lower counting of Pseudomonas, when compared with the control treatments and with ozonized water, both immediately after ozonation (day 1) and after 5 days of storage. The luminosity in the chicken meat samples treated with ozonized water was higher than that verified in the control treatments and with ozone gas, immediately after ozonation (day 1). A similar trend was observed in hue angle and color difference, in which the highest values were obtained for treatment with ozonized water. Based on the results obtained in this study, it was concluded that ozonated water can be used to remove P. paracarnis biofilm from stainless steel under static conditions and gaseous ozone is more efficient in the inactivation of P. paracarnis from chicken breast meat, when compared to ozonated water.

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Funding

The authors acknowledge the support from the National Council for Scientific and Technological Development (Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq, Grant Number: 405894/2021–0 and 406719/2023-3), the Research Support Foundation of the State of Minas Gerais (Fundação de Amparo à Pesquisa do Estado de Minas Gerais, FAPEMIG, Grant Number: APQ-00088–21 and APQ-01701–22), the Coordination for the Improvement of Higher Education Personnel (Coordenação de Aperfeiçoamento de Pessoal de Nível de Superior, CAPES), Funding Code 001, FARA, and Tertiary Education Trust Fund (TetFund).

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Authors and Affiliations

  1. Department of Agricultural Engineering, Universidade Federal de Viçosa, Viçosa, MG, 36570900, Brazil

    Ejima Akogwu Okolo, Ernandes Rodrigues de Alencar, Lêda Rita D’Antonino Faroni & Marcus Vinicius de Assis Silva

  2. Agricultural and Bio-Environmental Engineering Department, Federal Polytechnic Nekede, Imo State, P.M.B. 1036, Owerri, Nigeria

    Ejima Akogwu Okolo

  3. Department of Food Technology, INOVALEITE, Universidade Federal de Viçosa, Viçosa, MG, 36570900, Brazil

    Solimar Goncalves Machado, Nayara Aparecida da Silva Costa & Andressa Falqueto

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  1. Ejima Akogwu Okolo
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Contributions

EAO, ERA, LRDF, and SGM conceived and designed the experiment. EAO, ERA, SGM, LRDF, MVAS, AF, and NASC performed the experiments and analyzed the data. EAO, ERA, LRDF, and SGM wrote the manuscript. EAO, ERA, LRDF, and SGM revised the manuscript. ERA, LRDF, and SGM provided the funding for the study.

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Correspondence to Ernandes Rodrigues de Alencar.

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Okolo, E.A., de Alencar, E.R., Machado, S.G. et al. Ozonation for Pseudomonas paracarnis control: biofilm removal and preservation of chicken meat during refrigerated storage. Braz J Microbiol 54, 3051–3060 (2023). https://doi.org/10.1007/s42770-023-01157-1

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