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Encapsulation of probiotic Lactobacillus acidophilus ATCC 4356 in alginate–galbanum (Ferula Gummosa Boiss) gum microspheres and evaluation of the survival in simulated gastrointestinal conditions in probiotic Tahini halva

  • Biotechnology and Industrial Microbiology - Research Paper
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Abstract

One of the famous traditional confectionery products is Tahini halva. The aim of this study was the production of probiotic halva using free Lactobacillus acidophilus (FLA) and microencapsulated Lactobacillus acidophilus (MLA) with sodium alginate and galbanum gum as the second layer. The survival rate of MLA and FLA during heat stress, storage time, and simulation gastrointestinal condition in Tahini halva was assessed. The survival rates of MLA and FLA under heat stress were 50.13% and 34.6% respectively. During storage in Tahini halva, the cell viability loss was 3.25 Log CFU g−1 and 6.94 Log CFU g−1 for MLA and FLA, separately. Around 3.58 and 4.77 Log CFU g−1 bacteria were reduced after 6 h of exposure in simulated gastrointestinal conditions, for MLA and FLA respectively. These results suggest that the use of alginate and galbanum gum is a promising approach to protecting L. acidophilus against harsh environmental conditions.

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Data availability

The data are available from the corresponding author upon reasonable request.

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Acknowledgements

The authors thank the head of Fars Agriculture Research and Education Center for his support and encouragement in carrying out the work.

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Authors and Affiliations

  1. Fars Agricultural and Natural Resources Research and Education Center, AREEO, Post Box: 7155863511, Shiraz, Fars, Iran

    Seyed Saeed Sekhavatizadeh

  2. Food Science and Technology, Islamic Azad University, Sarvestan, Fars, Iran

    Forough Afrasiabi

  3. Department of Infectious Diseases, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran

    Zahra Montaseri

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  1. Seyed Saeed Sekhavatizadeh
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SSS carried out the experiments and wrote the MS, FA carried out the experiment, and ZM edited manuscript.

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Correspondence to Seyed Saeed Sekhavatizadeh.

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Sekhavatizadeh, S.S., Afrasiabi, F. & Montaseri, Z. Encapsulation of probiotic Lactobacillus acidophilus ATCC 4356 in alginate–galbanum (Ferula Gummosa Boiss) gum microspheres and evaluation of the survival in simulated gastrointestinal conditions in probiotic Tahini halva. Braz J Microbiol 54, 1589–1601 (2023). https://doi.org/10.1007/s42770-023-01074-3

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