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Detection and analysis of Shiga toxin producing and enteropathogenic Escherichia coli in cattle from Tierra del Fuego, Argentina

  • Veterinary Microbiology - Research Paper
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Abstract

Shiga toxin producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) are pathovars that affect mainly infants’ health. Cattle are the main reservoir of STEC. Uremic hemolytic syndrome and diarrheas can be found at high rates in Tierra del Fuego (TDF). This study aimed to establish the prevalence of STEC and EPEC in cattle at slaughterhouses in TDF and to analyze the isolated strains. Out of 194 samples from two slaughterhouses, STEC prevalence was 15%, and EPEC prevalence was 5%. Twenty-seven STEC strains and one EPEC were isolated. The most prevalent STEC serotypes were O185:H19 (7), O185:H7 (6), and O178:H19 (5). There were no STEC eae + strains (AE-STEC) or serogroup O157 detected in this study. The prevalent genotype was stx2c (10/27) followed by stx1a/stx2hb (4/27). Fourteen percent of the strains presented at least one stx non-typeable subtype (4/27). Shiga toxin production was detected in 25/27 STEC strains. The prevalent module for the Locus of Adhesion and Autoaggregation (LAA) island was module III (7/27). EPEC strain was categorized as atypical and with the ability to cause A/E lesion. The ehxA gene was present in 16/28 strains, 12 of which were capable of producing hemolysis. No hybrid strains were detected in this work. Antimicrobial susceptibility tests showed that all strains were resistant to ampicillin and 20/28 were resistant to aminoglycosides. No statistical differences could be seen in the detection of STEC or EPEC either by slaughterhouse location or by production system (extensive grass or feedlot). The rate of STEC detection was lower than the one reported for the rest of Argentina. STEC/EPEC relation was 3 to 1. This is the first study on cattle from TDF as reservoir for strains that are potentially pathogenic to humans.

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Acknowledgements

This study was performed at the Universidad de Buenos Aires, Facultad de Ciencias Veterinarias. The authors would like to thank the University and the Microbiology department for the assistance during the whole period of work.

Funding

This research was funded by a magister fellowship by Universidad de Buenos Aires, UBACyT 20020190100320, and PICT 2017–3360.

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Authors and Affiliations

  1. Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Instituto de Investigaciones en Epidemiología Veterinaria, Cátedra de Microbiología, Buenos Aires, Argentina

    Maria Paz Bonino, Ximena Blanco Crivelli, Cecilia Cundon, Alicia Broglio, Mariana Sanin & Adriana Bentancor

  2. Consejo Nacional de Investigaciones Científicas Y Técnicas (CONICET), Buenos Aires, Argentina

    Maria Paz Bonino & Alicia Broglio

  3. Departamento de Epidemiología, Ministerio de Salud de Tierra del Fuego, Ushuaia, Argentina

    Juan Facundo Petrina

  4. Dirección de Fiscalización Sanitaria, Ministerio de Salud de Tierra del Fuego, Ushuaia, Argentina

    Sebastian Galateo

  5. Federal University of São Paulo, São Paulo, Brazil

    Tania Aparecida Tardelli Gomes

  6. Departamento de Salud Pública, Facultad de Medicina, Universidad Nacional Autónoma de Mexico, Mexico City, Mexico

    Armando Navarro

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Bonino, M.P., Crivelli, X.B., Petrina, J.F. et al. Detection and analysis of Shiga toxin producing and enteropathogenic Escherichia coli in cattle from Tierra del Fuego, Argentina. Braz J Microbiol 54, 1257–1266 (2023). https://doi.org/10.1007/s42770-023-00958-8

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