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Modeling of Listeria innocua, Escherichia coli, and Salmonella Enteritidis inactivation in milk treated by gamma irradiation

  • Food Microbiology - Research Paper
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Abstract

Measuring microbial inactivation in food is useful for food technology as it allows for predicting the growth or death of microorganisms. This study aimed to investigate the effect of gamma irradiation on the lethality of microorganisms inoculated in milk, estimate the mathematical model of inactivation of each microorganism, and evaluate kinetic indices to determine the efficient dose in the treatment of milk. Raw milk samples were inoculated with cultures of Salmonella enterica subsp. enterica serovar Enteritidis (ATCC 13076), Escherichia coli (ATCC 8739), and Listeria innocua (ATCC 3309), irradiated at doses of 0, 0.5, 1, 1.5, 2, 2.5, and 3 kGy. The fitting of the models to the microbial inactivation data was performed using the GinaFIT software. The results demonstrated a significant effect of irradiation doses on the population of microorganisms, with the application of a dose of 3 kGy, a reduction of approximately 6 logarithmic cycles is observed for L. innocua and 5 for S. Enteritidis and E. coli. The model with the best fit was different for each microorganism studied: for L. innocua, the model was log-linear + shoulder; for S. Enteritidis and E. coli, the model that showed the best fit was the biphasic. The studied model fitted well (R2 ≥ 0.9; R2 adj. ≥ 0.9 and smallest RMSE values) for the inactivation kinetics. The lethality of the treatment, considering a reduction in the 4D value, was achieved with the predicted dose of doses of ±2.22, ±2.10, and ±1.77 kGy, for L. innocua, S. Enteritidis, and E. coli, respectively.

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Data Availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

Code Availability

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Acknowledgements

The authors thank UFVJM and CDTN for their support in conducting the experiments, the Coordination for the Improvement of Higher Education Personnel (CAPES) for resources provided to the Graduate Program in Food Science and Technology, and the Foundation for Research Support of the State of Minas Gerais (FAPEMIG) for the scholarship granted to graduate students.

Funding

This work was supported by the Foundation for Research Support of the State of Minas Gerais (FAPEMIG) (13100).

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Authors and Affiliations

  1. Department of Food Sciences, Federal University of Lavras, Aquenta Sol, Lavras, Minas Gerais, Brazil

    Fabiana Regina Lima

  2. Department of Nutrition, Federal University of Jequitinhonha and Mucuri Valley, Diamantina, Minas Gerais, Brazil

    Costa Sobrinho Paulo de Souza

  3. Institute of Science and Technology, Federal University of Jequitinhonha and Mucuri Valley, Diamantina, Minas Gerais, Brazil

    Larissa de Oliveira Ferreira Rocha & Poliana Mendes de Souza

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  1. Fabiana Regina Lima
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  2. Costa Sobrinho Paulo de Souza
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  3. Larissa de Oliveira Ferreira Rocha
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  4. Poliana Mendes de Souza
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Contributions

Fabiana Regina Lima: conceptualization; methodology; formal analysis; data curation; investigation; resources; writing—original draft.

Paulo de Souza Costa Sobrinho: resources; methodology; writing—review and editing.

Larissa de Oliveira Ferreira Rocha: resources; writing—review and editing.

Poliana Mendes de Souza: conceptualization; methodology; resources; writing—review and editing; supervision; project administration.

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Correspondence to Fabiana Regina Lima.

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Lima, F.R., de Souza Costa Sobrinho, P., de Oliveira Ferreira Rocha, L. et al. Modeling of Listeria innocua, Escherichia coli, and Salmonella Enteritidis inactivation in milk treated by gamma irradiation. Braz J Microbiol 54, 1047–1054 (2023). https://doi.org/10.1007/s42770-023-00931-5

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  • DOI: https://doi.org/10.1007/s42770-023-00931-5

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