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Elite Bradyrhizobium strains boost biological nitrogen fixation and peanut yield in tropical drylands

  • Soil and Agricultural Microbiology - Research Paper
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Abstract

Peanut (Arachis hypogaea L.) is an important crop for the family-based systems in the tropics, mainly in Brazil. In the Brazilian drylands, peanuts are cropped in low technological systems, and cheap and efficient technologies are needed to improve crop yield and sustainability. Despite this importance, few data are available on selecting efficient peanut rhizobia in experiments under different edaphoclimatic conditions. This work evaluated the agronomic efficiency and the biological nitrogen fixation (BNF) by two elite Bradyrhizobium strains under four different fields in the Brazilian semiarid region. We compared a new efficient strain Bradyrhizobium sp. ESA 123 with the reference strain B. elkanii SEMIA 6144, currently used in peanut rhizobial inoculants in Brazil. Besides the inoculated treatments, two uninoculated controls were assessed (with and without 80 kg ha−1 of N-urea). The BNF was estimated by the δ15N approach in three out of four field assays. BNF contribution was improved by inoculation of both Bradyrhizobium strains, ranging from 42 to 51% in Petrolina and 43 to 60% in Nossa Senhora da Glória. Peanuts’ yields benefited from the inoculation of both strains and N fertilization in all four assays. Nevertheless, the results showed the efficiency of both strains under different edaphoclimatic conditions, indicating the native strain ESA 123 as a potential bacterium for recommendation as inoculants for peanuts in Brazil, mainly in drylands.

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Acknowledgements

We are grateful for the several students and technicians that helped us during the field experiments.

Funding

The Brazilian Council for Scientific and Technological Development (CNPq), INCT–Plant Growth–Promoting Microorganisms for Agricultural Sustainability and Environmental Responsibility (CNPq/Fundação Araucária STI/CAPES INCT-MPCPAgro 465133/2014–4), and the Brazilian Agricultural Research Corporation (Embrapa 23.16.05.016.00.00) provided financial support.

The Pernambuco State Science Foundation (Facepe) awarded scholarships to the second author. Facepe (APQ 0271–5.01/19) and CNPq (316042/2020–0) also awarded the postdoc fellowship to the nineth author. The tenth and last authors are research fellows of CNPq (306812/2018–5 and 311218/2017–2, respectively).

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Authors and Affiliations

  1. Universidade Estadual da Paraíba (UEPB), PB, Campina Grande, Brazil

    Raphael Silva Jovino

  2. Colegiado de Farmácia, Universidade Federal do Vale do São Francisco (Univasf), PA, Petrolina, Brazil

    Thaíse Rosa da Silva & Ruth Terezinha Rodrigues

  3. Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana (UEFS), Feira de Santana, BA, Brazil

    Jackson Rafael de Sá Carvalho

  4. Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia (UNEB), Juazeiro, BA, Brazil

    Jussara Barboza de Alencar Cunha & Lindete Míria Vieira Martins

  5. Embrapa Algodão, Campina Grande, PB, Brazil

    Liziane Maria de Lima & Roseane Cavalcanti dos Santos

  6. Departamento de Agronomia, Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil

    Carolina Etienne de Rosália e Silva Santos & Ana Dolores Santiago de Freitas

  7. Fundação de Amparo à Pesquisa do Estado de Pernambuco (Facepe), Recife, PE, Brazil

    Paula Rose de Almeida Ribeiro

  8. Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brasília, DF, Brazil

    Paula Rose de Almeida Ribeiro

  9. Embrapa Semiárido, BR 428, Km 152, Zona Rural, CP 23, Petrolina, PE, CEP: 56302-970, Brazil

    Paula Rose de Almeida Ribeiro & Paulo Ivan Fernandes-Júnior

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  1. Raphael Silva Jovino
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  2. Thaíse Rosa da Silva
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  3. Ruth Terezinha Rodrigues
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  6. Liziane Maria de Lima
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  7. Roseane Cavalcanti dos Santos
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  8. Carolina Etienne de Rosália e Silva Santos
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  10. Ana Dolores Santiago de Freitas
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  11. Lindete Míria Vieira Martins
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  12. Paulo Ivan Fernandes-Júnior
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Contributions

LML, RCS, CERSS, ADSF, LMVM, and PIF-J conceived the research project; RSJ and PIF-J implemented and collected all field assays; RSJ, TRS, RTR, JRSC, and JBAC processed and analyzed all plant material collected in the fields; ADSF and CERSS conducted the isotopic analyzes; PRAR, ADSF, and PIF-J analyzed all data; PRAR and PIF-J wrote the manuscript; all authors read and approved the final manuscript version.

Corresponding author

Correspondence to Paulo Ivan Fernandes-Júnior.

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Jovino, R.S., da Silva, T.R., Rodrigues, R.T. et al. Elite Bradyrhizobium strains boost biological nitrogen fixation and peanut yield in tropical drylands. Braz J Microbiol 53, 1623–1632 (2022). https://doi.org/10.1007/s42770-022-00792-4

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  • DOI: https://doi.org/10.1007/s42770-022-00792-4

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