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Biochemical characterization and biological properties of mycelium extracts from Lepista sordida GMA-05 and Trametes hirsuta GMA-01: new mushroom strains isolated in Brazil

  • Fungal and Bacterial Physiology - Research Paper
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Abstract

The objective of this study was to evaluate the antioxidant activity, determine and quantify the phenolic compounds and other compounds, and evaluate the cellular cytotoxicity of mycelium extracts of two new Basidiomycete mushrooms strains isolated in Brazil and identified as Lepista sordida GMA-05 and Trametes hirsuta GMA-01. Higher amounts of proteins, free amino acids, total and reducing carbohydrates, and phenolic compounds as chlorogenic, ferulic, caffeic, and gallic acids were found in extracts of T. hirsuta and L. sordida. Protocatechuic acid was found only in aqueous extracts of L. sordida. The TLC of the extracts showed the predominance of glucose and smaller amounts of xylose. It was observed through UPLC-MS higher amounts of phenolic compounds. The aqueous extract from T. hirsuta had the most noteworthy results in the antioxidant assays, especially the ABTS test. The cytotoxic activity was evaluated using two different cell lineages and showed higher toxicity for L. sordida in macrophages J774-A1. However, in Vero cells, it was 12.6-fold less toxic when compared to T. hirsuta. Thus, both mushrooms show potential as functional foods or additives, presenting phenolic content, antioxidant activity, and low cytotoxic activity in the tested cells.

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Acknowledgements

The authors thank to the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) for the doctorate grant awarded to A.G. Contato (Process nº 2017/25862-6); for the postdoctoral grant awarded to T.B. Oliveira (Process nº 2017/9000-4); and the financial support from National Institute of Science and Technology of Bioethanol (Process FAPESP 2014/50884-5) and research assistance in partnership with the Fundação para a Ciência e a Tecnologia (FCT) (Process FAPESP 2018/07522-6) and Conselho Nacional de Desenvolvimento Científico (CNPq), process 574002/2008-1 and 465319/2014-9. M.L.T.M. Polizeli (process 301963/2017-7), R.M. Peralta, L.A. Moraes, and C.V. Nakamura are Research Fellows of CNPq. G.M. Aranha and G.G. Ortolan had an undergraduate research grant awarded by CNPq, and J.S Salgado had a postdoctoral grant awarded. K.M. Retamiro and E.J. Crevelin are recipients of grants from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) finance code 001.

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Authors and Affiliations

  1. Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil

    Guilherme Mauro Aranha, Tássio Brito de Oliveira, Guilherme Guimarães Ortolan & Maria de Lourdes Teixeira de Moraes Polizeli

  2. Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil

    Alex Graça Contato & Maria de Lourdes Teixeira de Moraes Polizeli

  3. Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, Maringá , Paraná, Brazil

    Karina Miyuki Retamiro & Celso Vataru Nakamura

  4. Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil

    José Carlos dos Santos Salgado, Eduardo José Crevelin & Luiz Alberto Beraldo de Moraes

  5. Departamento de Bioquímica, Universidade Estadual de Maringá, Maringá, Paraná, Brazil

    Rosane Marina Peralta

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  1. Guilherme Mauro Aranha
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Aranha, G.M., Contato, A.G., Salgado, J.C.d. et al. Biochemical characterization and biological properties of mycelium extracts from Lepista sordida GMA-05 and Trametes hirsuta GMA-01: new mushroom strains isolated in Brazil. Braz J Microbiol 53, 349–358 (2022). https://doi.org/10.1007/s42770-021-00670-5

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