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Growth-promoting effects of Bradyrhizobium soybean symbionts in black oats, white oats, and ryegrass

  • Environmental Microbiology - Research Paper
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Abstract

Although inoculating soybean with rhizobia for biological nitrogen fixation is a common practice in agriculture, rhizobia are also known to associate with grasses. In this study, we evaluate the potential utility of the rhizobial strains SEMIA 587 and 5019 (Bradyrhizobium elkanii), 5079 (Bradyrhizobium japonicum), and 5080 (Bradyrhizobium diazoefficiens), recommended for Brazilian soybean inoculation, in colonizing black oat plants and promoting growth in black and white oats, and ryegrass. Inoculation of white oats with SEMIA 587 increase the seed germination (SG) by 32.09%, whereas the SG of black oats inoculated with SEMIA 587 and 5019 increased by 40.38% and 37.85%, respectively. Similarly, inoculation of ryegrass with all strains increased SG values between 24.63 and 27.59%. In addition, white oats with SEMIA 587 and 5080 had root areas significantly superior to those in other treatments, whereas inoculation with SEMIA 5079 and 5080 resulted in the highest volume of roots. Likewise, SEMIA 5079 and 5080 significantly increased the length, volume, and area of black oats roots, whereas SEMIA 587 increased the volume, area, and dry mass of roots and shoot. Inoculation in ryegrass with SEMIA 587 significantly increased the root volume. Moreover, most strains transformed with gfp and gus were observed to colonize the roots of black oats. Collectively, the findings of this study indicate that rhizobial strains recommended for inoculation of soybean can also be used to promote the growth of the three assessed grass species, and are able to colonize the roots of black oats.

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Funding

This work was financed by a grant and fellowships from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq/Brazil).

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Authors and Affiliations

  1. Departamento de Solos, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 7712, Porto Alegre, RS, CEP 91540-000, Brazil

    Carolina Leal de Castilho, Lucas Zulpo & Enílson Luiz Saccol de Sá

  2. Departamento de Genética, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Porto Alegre, RS, CEP 91501-970, Brazil

    Camila Gazolla Volpiano, Adriana Ambrosini & Luciane Passaglia

  3. Departamento de Diagnóstico e Pesquisa Agropecuária (antiga FEPAGRO) da Secretaria da Agricultura, Pecuária e Desenvolvimento Rural (SEAPDR) do Rio Grande do Sul, Rua Gonçalves Dias, 570, Porto Alegre, RS, CEP 90130-060, Brazil

    Anelise Beneduzi

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  1. Carolina Leal de Castilho
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Contributions

Material and methods preparation, and analysis were performed by Carolina Leal de Castilho, Camila Gazolla Volpiano, Lucas Zulpo, Adriana Ambrosini, and Anelise Beneduzi. Grant and fellowships acquisition were provided by Enílson Luiz Saccol de Sá, and Luciane Passaglia. The manuscript was written by Carolina Leal de Castilho, Enílson Luiz Saccol de Sá, and Anelise Beneduzi. All authors commented on previous versions of the manuscript, and approved the final manuscript.

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de Castilho, C.L., Volpiano, C.G., Ambrosini, A. et al. Growth-promoting effects of Bradyrhizobium soybean symbionts in black oats, white oats, and ryegrass. Braz J Microbiol 52, 1451–1460 (2021). https://doi.org/10.1007/s42770-021-00523-1

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