Abstract
Essential oils of three plants, namely Haplophyllum tuberculatum, Psidium guajava and Pinus roxburghii were obtained by hydrodistillation and analyzed by gas chromatography/mass spectrometry (GC/MS). Contact toxicity assay was used to evaluate the insecticidal potential of isolated essential oils against three stored product insets, Sitophilus oryzae, Tribolium castaneum and Trogoderma granarium. The efficacy of the oils for controlling S. oryzae in stored wheat at three application rates (0.5, 1.0 and 5.0 g/kg grain) was also evaluated. GC/MS analyses confirmed the main compounds to be cis-sabinene hydrate (27.21%) in H. tuberculatum, d-limonene (30.73%) in P. guajava, and α-pinene (39.16%) in P. roxburghii. The oil of H. tuberculatum revealed the highest contact toxicity against the adults of T. castaneum, T. granarium and S. oryzae displaying LC50 values of 0.13, 0.048 and 0.11 mg/cm2, respectively. Likewise, the oil of H. tuberculatum showed higher toxicity to T. granarium larvae than P. guajava and P. roxburghii oils. In grain protectant assay, the three oils induced complete adult mortality of S. oryzae after 7 days of exposure to treated wheat with 5.0 g/kg. Furthermore, the oils induced significant progeny decrease of S. oryzae after 45 and 90 days of treatment with complete suppression (100%) of progeny at 5.0 g/kg. The essential oils also prevent wheat damage for 90 days. According to the results described here, the three essential oils may be developed as a biorational tool to protect the stored products against insects.
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Abdelgaleil SAM, Mohamed MIE, Badawy MEI, El-arami SAA (2009) Fumigant and contact toxicities of monoterpenes to Sitophilus oryzae (L.) and Tribolium castaneum (Herbst) and their inhibitory effects on acetylcholinesterase activity. J Chem Ecol 35:225–232
Abdelgaleil SAM, Mohamed MIE, Shawir MS, Abou-Taleb HK (2016) Insecticidal and biochemical effects of essential oils on the rice weevil, Sitophilus oryzae L. (Coleoptera: Curculionidae). J Pest Sci 89:219–229
Abou-Taleb HK, Mohamed MIE, Shawir MS, Abdelgaleil SAM (2016) Insecticidal properties of essential oils against Tribolium castaneum (Herbst) and their inhibitory effects on acetylcholinesterase and adenosine triphosphatases. Nat Prod Res 30:710–714
Al-Burtamani SKS, Fatope MO, Marwah RG, Onifade AK, Al-Saidi SH (2005) Chemical composition, antibacterial and antifungal activities of the essential oil of Haplophyllum tuberculatum from Oman. J Ethnopharmacol 96:107–112
Al-Rehaily AJ, Alqasoumi SI, Yusufoglu HS, Al-Yahya MA, Demirci B, Tabanca N, Wedge DE, Demirci F, Bernier UR, Becnel JJ, Temel HE, Başer KHC (2014) Chemical composition and biological activity of Haplophyllum tuberculatum Juss. essential oil. J Essent Oil bear Pl 17:452–459
Al-Yousuf MH, Bashir AK, Veres K, Dobos A, Nagy N, Mathe I, Blunden G, Vera JR (2005) Essential oil of Haplophyllum tuberculatum (Forssk.) A. Juss. from the United Arab Emirates. J Essent Oil Res 17:519–521
Arain A, Sherazi STH, Mahesar SA, Sirajuddin (2019) Essential oil from Psidium guajava leaves: an excellent source of β- caryophyllene. Nat Prod Commun 1–5. https://doi.org/10.1177/1934578X19843007
Asawalam EF, Hassanali A (2006) Constituents of the essential oil of Vernonia amygdalina as maize weevil protectants. Tropical and Subtropical Agroecosystems 6:95–102
Athanassiou CG, Kavallieratos NG, Economou LP, Dimizas CB, Vayias BJ (2005) Persistence and efficacy of three diatomaceous earth formulations against Sitophilus oryzae (Coleoptera: Curculionidae) on wheat and barley. J Econ Entomol 98:1404–1412
Athanassiou CG, Kavallieratos NG, Boukouvala MC, Mavroforos ME, Kontodimas DCJ (2015) Efficacy of alpha-cypermethrin and thiamethoxam against Trogoderma granarium Everts (Coleoptera: Dermestidae) and Tenebrio molitor L. (Coleoptera: tenebrionidae) on concrete. J Stored Prod Res 62:101–107
Bekele AJ, Obeng-Ofori D, Hassanali A (1997) Evaluation of Ocimum kenyense (Ayobangira) as a source of repellent, toxicants and protectants in storage against three stored product insect pests. J Appl Entomol 121:169–173
Bhagat M, Bandral A, Bashir M, Bindu K (2018) GC–MS analysis of essential oil of Pinus roxburghii Sarg. (Chir pine) needles and evaluation of antibacterial and anti-proliferative properties. Indian J Nat Prod Resou 9:34–38
Borah A, Pandey SK, Haldar S, Lal M (2019) Chemical composition of leaf essential oil of Psidium guajava L. from north east India. J Essent Oil Bear Pl 22:248–253
Chen H-C, Sheu M-J, Lin L-Y, Wu C-M (2007) Chemical composition of the leaf essential oil of Psidium guajava L. from Taiwan. J Essent Oil Res 19:345–347
Cowan MM (1999) Plant products as antimicrobial agents. Clin Microbiol Rev 12:564–582
De Lira C, Pontual EV, De Albuquerque LP, Paiva LM, Paiva PG, De Oliveira JV, Napoleão TH, Navarro DMAF (2015) Evaluation of the toxicity of essential oil from Alpinia purpurata inflorescences to Sitophilus zeamais (maize weevil). Crop Protect 71:95–100
Derbalah AS, Ahmed SI (2011) Oil and powder of spearmint as an alternative to Sitophilus oryzae chemical control of wheat grains. J Plant Protect Res 51:145–150
Eliopoulos P (2013) New approaches for tackling the Khapra beetle. CAB Rev 8:1–13
El-Naggar EMB, El-Darier SM, Abdalla A, El-Mekanen S, Švajdlenka E, Zemlièka M (2014) Chemical composition of essential oil of Haplophyllum tuberculatum (Rutaceae) grow wild in different habitats of Egypt. Global J Pharmacol 8:385–393
Finney DJ (1971) Probit analysis, 3rd edn. Cambridge University Press, London, p 318
Gad HA, Al-Anany MSM, Abdelgaleil SAM (2020) Enhancement the efficacy of spinosad for the control Sitophilus oryzae by combined application with diatomaceous earth and Trichoderma harzianum. J Stored Prod Res 88:101663
Garcia M, Donadel OJ, Ardanaz CE, Tonn CE, Sosa ME (2005) Toxic and repellent effects of Baccharis salicifolia essential oil on Tribulium castaneum. Pest Manag Sci 61:612–618
Gewali MB (2008) Aspects of Traditional Medicine in Nepal. Institute of Natural Medicine. University of Toyama, Japan, pp 19–20
Ghasemzadeh S, Mirfakhraie S, Najafzadeh R (2018) Fumigant toxicity of Haplophyllum tuberculatum (Rutaceae) and Nepeta crispa (Lamiaceae) on the Indian meal moth. Julius-Kuhn-Archiv 463:687–695
Ghimire MN, Myers SW, Arthur FH, Phillips TW (2017) Susceptibility of Trogoderma granarium Everts and Trogoderma inclusum LeConte (Coleoptera: Dermestidae) to residual contact insecticides. J Stored Prod Res 72:75–82
Gupta AK, Behal SR, Awasthi BK, Verma RA (1999) Screening of some maize genotypes against Sitophilus oryzae. Indian J Entomol 61:265–268
Hamdani FZ, Allem R, Meziane M, Setti B, Ali AS, Bourai M (2015) Chemical composition and antifungal activity of essential oils of Algerian citrus. Afr J Biotechnol 14:1048–1055
Hashemi SM, Safavi SA (2012) Chemical constituents and toxicity of essential oils of oriental arborvitae, Platycladus orientalis (L.) Franco, against three stored-product beetles. Chil J Agric Res 72:188–194
Hernández-Cruz J, Luna-Cruz A, Loera-Alvarado E, Villanueva-Sánchez E, Landero-Valenzuela N, Zárate-Nicolás BH, Diego-Nava F, Granados-Echegoyen CA (2019) Efficiency of the essential oil of Porophyllum linaria (Asteraceae) a Mexican endemic plant against Sitophilus zeamais (Coleoptera: Curculionidae). J Insect Sci 1–9. doi: https://doi.org/10.1093/jisesa/iez079
Hosny AH, Hasan NE, Zayed GMM, Frawila HAA (2018) Bioactivity of Marjoram oil and powder against the rice weevil, Sitophilus oryzae. Res Rev J Botan Sci 7:14–21
Isman MB (2006) Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Ann Rev Entomol 51:45–66
Jacob LD, Ntungwen FC, Christopher S, Wilson AA, Roli TT, Nchiwan NE (2018) Effectiveness of essential oils from Ngaoundere against post-harvest insect and fungal pests of maize. Julius-Kuhn-Archiv 463:839–847
Janaki S, Zandi-Sohani N, Ramezani L, Szumny A (2018) Chemical composition and insecticidal efficacy of Cyperus rotundus essential oil against three stored product pests. Int Biodeterior Biodegrad 133:93–98
Javidnia K, Miri R, Banani A (2006) Volatile oil constituents of Haplophyllum tuberculatum (Forssk.) A. Juss. (Rutaceae) from Iran. J Essent Oil Res 18:355–356
Joshi R, Tiwari SN (2019) Fumigant toxicity and repellent activity of some essential oils against stored grain pest Rhyzopertha dominica (Fabricius). J Pharm Phytochem 8:59–62
Kaushik P, Kaushik D, Khokra SL (2013) Ethnobotany and phytopharmacology of Pinus roxburghii Sarg: A plant review. J Integr Med 11:371–376
Khadhri A, El Monkni R, Almedia C, Nogueria J, Araujo MEM (2014) Chemical composition of essential oil of Psidium guajava L. growing in Tunisia. Ind Crop Prod 52:29–31
Kim S-W, Kang J, Park I-K (2013) Fumigant toxicity of Apiaceae essential oils and their constituents against Sitophilus oryzae and their acetylcholinesterase inhibitory activity. J Asia-Pac Entomol 16:443–448
Kouninki H, Hance T, Noudjou FA, Lognay G, Malaisse F, Ngassoum MB, Mapongmetsem PM, Ngamo LST, Haubruge E (2007) Toxicity of some terpenoids of essential oils of Xylopia aethiopica from Cameroon against Sitophilus zeamais Motschulsky. J Appl Entomol 131:269–274
Mohamed MIE, Abdelgaleil SAM (2008) Chemical composition and insecticidal potential of the essential oils from Egyptian plants against Sitophilus oryzae (L.) (Coleoptera: Curculionidae) and Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). App Entomol Zool 43:599–607
Nenaah GE (2014) Chemical composition, insecticidal and repellence activities of essential oils of three Achillea species against the Khapra beetle (Coleoptera: Dermestidae). J Pest Sci 87:273–283
Nenaah GE, Ibrahim SIA (2011) Chemical composition and the insecticidal activity of certain plants applied as powders and essential oils against two stored-products coleopteran beetles. J Pest Sci 84:393–402
Norambuena C, Silva G, Urbina A, Figueroa I, Rodríguez-Maciel JC (2016) Insecticidal activity of Laureliopsis philippiana (Looser) Schodde (Atherospermataceae) essential oil against Sitophilus spp. (Coleoptera Curculionidae) Chil J Agric Res 76:330-336
Nouri-Ganbalani G, Borzoui E (2107) Acute toxicity and sublethal effects of Artemisia sieberi Besser on digestive physiology, cold tolerance and reproduction of Trogoderma granarium Everts (Col.: Dermestidae. J Asia-Pac Entomol 20:285–292
Okunola CO, Ofuya TI (2007) Effect of some essential oils on insect infestation of stored maize and cowpea. Proc Afr Crop Sci 8:1003–1007
Ortiz A, Silva G, Urbina A, Zapata N, Rodriguez J, Lagunes A (2012) Bioactivity of tepa (Laureliopsis Philippiana (Looser) Shodde) power to Sitophilus zeamais Motschulsky control in laboratory. Chil J Agric Res 72:68–73
Pierattini EC, Bedini S, Venturi F, Ascrizzi R, Flamini G, Bocchino R, Girardi J, Giannotti P, Ferroni G, Conti B (2019) Sensory quality of essential oils and their synergistic effect with diatomaceous earth, for the control of stored grain insects. Insects 10:114
Pinho AI, Wallau GL, Nunes ME, Leite NF, Tintino SR, da Cruz LC, da Cunha FA, da Costa JG, Douglas Melo, Coutinho H, Posser T, Franco JL (2014) Fumigant activity of the Psidium guajava Var. pomifera (Myrtaceae) essential oil in Drosophila melanogaster by means of oxidative stress. Oxid Med Cell Longev 2014, 696785
Rajendran S, Sriranjinia V (2008) Plant products as fumigants for stored-product insect control. J Stored Prod Res 44:126–135
Saad MMG, Abdelgaleil SAM (2018) Effectiveness of monoterpenes and phenylpropenes against Sitophilus oryzae L. (Coleoptera: Curculionidae) on stored wheat. J Asia-Pac Entomol 21:1153–1158
Saad MMG, Abou-Taleb HK, Abdelgaleil SAM (2018) Insecticidal activities of monoterpenes and phenylpropenes against Sitophilus oryzae and their inhibitory effects on acetylcholinesterase and adenosine triphosphatases. Appl Entomol Zool 53:173–181
Saad MMG, El-Deeb DA, Abdelgaleil SAM (2019) Insecticidal potential and repellent and biochemical effects of phenylpropenes and monoterpenes on the red flour beetle, Tribolium castaneum Herbst. Environ Sci Poll Res 26:6801–6810
Sahaf BZ, Moharramipour S, Meshkatalsadat MH (2008) Fumigant toxicity of essential oil from Vitex pseudo-negundo against Tribolium castaneum (Herbst) and Sitophilus oryzae (L). J Asia-Pac Entomol 11:175–179
Salem MZM, Ali HM, Basalah MO (2014) Essential oils from wood, bark, and needles of Pinus roxburghii Sarg. from Alexandria, Egypt: antibacterial and antioxidant activities. BioResources 9:7454–7466
Santos FA, RaoVSN, Silveira ER (1998) Investigations on the antinociceptive effect of Psidium guajava leaf essential oil and its major constituents. Phytother Res 12:24–27
Satyal P, Paudel P, Lamichhane B, Setzer WN (2015) Leaf essential oil composition and bioactivity of Psidium guajava from Kathmandu, Nepal. Am J Essent Oil Nat Prod 3:11–14
Satyal P, Paudel P, Raut J, Deo A, Dosoky NS, Setzer WN (2013) Volatile constituents of Pinus roxburghii from Nepal. Phcog Res 5:43–48
Seada MA, Abo Arab R, Adel I, Seif AI (2016) Bioactivity of essential oils of basil, fennel, and geranium against Sitophilus oryzae and Callosobruchus maculatus: evaluation of repellency, progeny production and residual activity. Egypt J Exp Biol (Zool) 12:1–12
Stefanazzi N, Teodoro S, Ferrero A (2011) Composition and toxic, repellent and feeding deterrent activity of essential oils against the stored-grain pests Tribolium castaneum (Coleoptera: Tenebrionidae) and Sitophilus oryzae (Coleoptera: Curculionidae). Pest Manag Sci 67:639–646
Suthisut D, Fields PG, Chandrapatya A (2011) Fumigant toxicity of essential oils from three Thai plants (Zingiberaceae) and their major compounds against Sitophilus zeamais, Tribolium castaneum and two parasitoids. J Stored Prod Res 47:222–230
Tadesse TM, Subramanyam B (2018) Efficacy of filter cake and Triplex powders from Ethiopia applied to wheat against Sitophilus zeamais and Sitophilus oryzae. J Stored Prod Res 79:40–52
Tapondjou AL, Adler C, Fontem DA, Bouda H, Reichmuth C (2005) Bioactivities of cymol and essential oils of Cupressus sempervirens and Eucalyptus saligna against Sitophilus zeamais Motschulsky and Tribolium confusum du Val. J Stored Prod Res 41:91–102
Varma J, Dubey NK (2001) Efficacy of essential oils of Caesulia axillaris and Mentha vriensis against some storage pests causing biodeterioration of food commodities. Int J Food Microbiol 68:207–210
Waliwitiya R, Kennedy C, Lowenberger C (2008) Larvicidal and oviposition altering activity of monoterpenoids, trans-anethole and rosemary oil to the yellow fever mosquito Aedes aegypti (Diptera: Culicidae). Pest Manag Sci 65:241–248
Yoon C, Kang S-H, Jang S-A, Kim Y-J, Kim G-H (2007) Repellent efficacy of caraway and grapefruit oils for Sitophilus oryzae (Coleoptera: Curculionidae). J Asia-Pac Entomol 10:263–267
Zafar I, Fatima A, Khan SJ, Rehman Z, Mehmud S (2010) GC-MS studies of needles essential oil of Pinus roxburghii and their antimicrobial activity from Pakistan. Elec. J Environ Agric Food Chem 9:468–473
Zohair HM, Hamed JJ, May A, Ali ZS (1989) Insecticidal effects of Haplophyllum tuberculatum against Cluex quinquefasciatus. Int J Crude Drug Res 27:17–21
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This work was partially supported by the Alexandria University Research Fund (ALEX-REP).
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Saad, M.M.G., El Gendy, A.N.G., Elkhateeb, A.M. et al. Insecticidal properties and grain protective efficacy of essential oils against stored product insects. Int J Trop Insect Sci 42, 3639–3648 (2022). https://doi.org/10.1007/s42690-022-00750-5
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DOI: https://doi.org/10.1007/s42690-022-00750-5