Abstract
This study was carried out to have a closer investigation on taxonomic position of the northeastern Pakistani Ajania fruticulosa with 3 genera viz., Artemisia, Ajania and Chrysanthemum. To achieve this, the nrDNA-ITS (nuclear ribosomal internal transcribed spacer) region was newly sequenced for A. fruticulosa (strain PMNH-041623) and pooled with already deposited ITS sequences in the GenBank from the three genera. In the resulting ITS phylogenetic maximum likelihood (ML) tree, A. fruticulosa specified close resemblance and appeared in a clade encompassing Ajania and Chrysanthemum species. The ITS phylogeny with an emphasis on three genera using ML approach proposed that Ajania and Chrysanthemum have close affinities with each other that might create confusions in their generic circumscription. Nevertheless, the monopyly of Artemisia genus was evident in the current ITS phylogeny. On the basis of outcomes of this study, it is proposed that bigger revised phylogenies with large number of taxa with the inclusion of northeastern Pakistani A. fruticulosa are necessary to clarify species delimitation and the intergeneric relationships in Ajania, Artemisia and Chrysanthemum. It is further recommended that comprehensive field surveys should be accompanied to acknowledge the distribution of more Ajania species across the northeastern Gilgit-Baltistan Pakistan.
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References
Bremer K, Humphries CJ (1993) Generic monograph of the Asteraceae Anthemideae. Bull Br Mus Lond (Bot) 23:71–177
Bremer K (1994) Asteraceae. Cladistics and classification. Timber Press, Portland
Guo YP, Ehrendorfer F, Samuel R (2004) Phylogeny and systematic of Achillea (Asteraceae–Anthemideae) inferred from nrDNA ITS and plastid trnL-F DNA sequences. Taxon 53:657–672. https://doi.org/10.2307/4135441
Himmelreich S, Kallersjo M, Eldenas P, Oberprieler C (2008) Phylogeny of southern hemisphere Compositae–Anthemideae based on nrDNA and cpDNA ndhF sequences information. Pl Syst Evol 272:131–153. https://doi.org/10.1007/s00606-007-0634-y
Hussain A (2019) Distribution and molecular phylogeny of Artemisia plants from Gilgit-Baltistan, Pakistan. Doctoral dissertation, International Islamic University Islamabad Pakistan. Available at http://prr.hec.gov.pk/jspui/handle/123456789/11070
Hussain A, Hayat MQ, Sahreen S, Bokhari SAI (2019a) Unveiling the foliar epidermal anatomical characteristics of genus Artemisia (Asteraceae) from northeast (Gilgit-Baltistan), Pakistan. Int J Agric Biol 21:630–638. https://doi.org/10.17957/IJAB/15.0938
Hussain A, Potter D, Kim S, Hayat MQ, Bokhari SAI (2019b) Molecular phylogeny of Artemisia (Asteraceae-Anthemideae) with emphasis on undescribed taxa from Gilgit-Baltistan (Pakistan) based on nrDNA (ITS and ETS) and cpDNA (psbA-trnH) sequences. Pl Ecol Evol 152:507–520. https://doi.org/10.5091/plecevo.2019.1583
Hussain A, Potter D, Hayat MQ, Sahreen S, Bokhari SAI (2019c) Pollen morphology and its systematic implication on some species of Artemisia L. from Gilgit-Baltistan Pakistan. Bangl J Pl Taxon 26:157–168. https://doi.org/10.3329/bjpt.v26i2.44576
Hussain A, Hayat MQ, Bokhari SAI (2020a) New distribution report on the alien species Artemisia verlotiorum Lamotte (Asteraceae- Anthemideae) from Gilgit-Baltistan region of Pakistan. Pak J Weed Sci Res 26:167–178. https://doi.org/10.28941/pjwsr.v26i2.826
Hussain A, Hayat MQ, Bokhari SAI (2020b) Artemisia chamaemelifolia Vill: a rare species of genus Artemisia (Asteraceae-Anthemideae) now present in the northeast (Gilgit-Baltistan) region of Pakistan. Biologia 76:47–54. https://doi.org/10.2478/s11756-020-00610-y
Kornkven AB, Watson LE, Estes JR (1998) Phylogenetic analysis of Artemisia section Tridentatae (Asteraceae) based on sequences from the internal transcribed spacers (ITS) of nuclear ribosomal DNA. Amer J Bot 86:1787–1795. https://doi.org/10.2307/2446513
Kornkven AB, Watson LE, Estes JR (1999) Molecular phylogeny of Artemisia section Tridentatae (Asteraceae) based on chloroplast DNA restriction site variation. Syst Bot 24:69–84. https://doi.org/10.2307/2419387
Kubitzki K (2007) The families and genera of vascular plants. In: Kadereit JW, Jeffrey C (eds) Flowering plants. Eudicots. Asterales, vol VIII. Springer-Verlag, Berlin, Heidelberg, pp 357–358
Liang J, Guo S, You C, Zhang W, Wang C, Geng Z, Deng Z, Du S, Zhang J (2016) Chemical constituents and insecticidal activities of Ajania fruticulosa essential oil. Chem Biodiv 13:1053–1057. https://doi.org/10.1002/cbdv.201500377
Liang J, Liu Y, Zhang X, Zhang L, Chen Y, Li Y, Zhang H, Kong W, Du S (2018) Antagonistic activity of essential oils and their main constituents extracted from Ajania fruticulosa and A. potaninii against Ditylenchus destructor. Nematology 20:911–916. https://doi.org/10.1163/15685411-00003185
Li J, He N, Wei X, Gao Y, Zuo Y (2015) Changes in temperature sensitivity and activation energy of soil organic matter decomposition in different Qinghai-Tibet Plateau grasslands. PLoS ONE 10:e0132795. https://doi.org/10.1371/journal.pone.0132795
Martın J, Torrell M, Korobkov AA, Valle`s J (2003) Palynological features as a systematic marker in Artemisia L. and related genera (Asteraceae, Anthemideae), II: implications for subtribe Artemisiinae delimitation. Pl Biol 5:85–93. https://doi.org/10.1055/s-2003-37979
Masuda Y, Yukawa T, Kondo K (2009) Molecular phylogenetic analysis of members of Chrysanthemum and its related genera in the tribe Anthemideae, the Asteraceae in East Asian on the basis of the internal transcribed spacer (ITS) region and external transcribed spacer (ETS) region of nr DNA. Chrom Bot 4:25–36. https://doi.org/10.3199/iscb.4.25
Muldashev AA (1982) New taxa of the genus Ajania (Asteraceae–Anthemideae). Botan Zhurn 67:1528–1532
Muldashev AA (1983) Kriticheskii peresmotr roda Ajania (Asteraceae–Anthemideae). [A critical review of the genus Ajania (Asteraceae). Botan Zhurn 68:207–214
Oberprieler C (2001) Phylogenetic relationships in Anthemis L. (Compositae, Anthemideae) based on nrDNA ITS sequence variation. Taxon 50:745–762. https://doi.org/10.2307/1223705
Oberprieler C, Himmelreich S, Vogt R (2007) A new subtribal classification of the tribe Anthemideae (Compositae). Willdenowia 37:89–114. https://doi.org/10.3372/wi.37.37104
Pellicer J, Hidalgo O, Garcia S, Garnatje T, Korobkov AA, Vallès J, Martín J (2009) Palynological study of Ajania and related genera. Anthemideae) Botan J Linn Soci 161:171–189. https://doi.org/10.1111/j.1095-8339.2009.00998.x. Asteraceae
Poljakov PP (1955) Botanicheskie Materialy Gerbariia Botanicheskogo Instituta imeni V. L. Koma Akad Nauk SSSR 17:419
Potter D, Eriksson T, Evans RC, Oh S, Smedmark JEE, Morgan DR, Kerr M, Robertson KR, Arsenault M, Dickinson TA, Campbell CS (2007) Phylogeny and classification of Rosaceae. Pl Syst Evol 266:5–43. https://doi.org/10.1007/s00606-007-0539-9
Sanz M, Vilatersana R, Hidalg O, Garcia-Jacas N, Susanna A, Schneeweiss MG, Vallès J (2008) Molecular phylogeny and evolution of floral characters of Artemisia and allies (Anthemideae, Asteraceae): evidence from nrDNA ETS and ITS sequences. Taxon 57:66–78
Shatar S, Staneva J, Altantsetseg S, Todorova M (2010) Essential oil composition of three Ajania species from Mongolia. J Essent Oil Bear Pl 13:460–464. https://doi.org/10.1080/0972060X.2010.10643850
Tantray YR, Jan I, Wani MS, Singhal VK, Gupta RC (2021) Chromosome numbers and meiotic behaviour in some species of Asteraceae from high altitudinal regions of Kashmir Himalayas. J Asia-Pac Biodiv 14:590–606. https://doi.org/10.1016/j.japb.2021.07.002
Tikhonova EV, Atazhanova GA, Raldugin VA, Bagryanskaya IU, Gatilov YV, Shakirov MM, Adekenov SM (2006) 2,12′-bis-hamazulenyl from Ajania fruticulosa essential oil. Chem Nat Comp 42:298–300. https://doi.org/10.1007/s10600-006-0103-9
Torrell M, Garcia-Jacas N, Susanna A, Valle`s J (1999) Phylogeny in Artemisia (Asteraceae, Anthemideae) inferred from nuclear ribosomal DNA (ITS) sequences. Taxon 48:721–736. https://doi.org/10.2307/1223643
Tzvelev NN (1961) Rod 1544. Ajania Poljak. In: Shiskin BK, Bobrov EG (eds) Flora of the USSR, vol 26. Akademiya Nauk, Leningrad, pp 458–473
Valle`s J, Torrell M, Garnatje T, Garcia-Jacas S, Vilatersana R, Susanna A (2003) The genus Artemisia and its allies: phylogeny of the subtribe Artemisiinae (Asteraceae, Anthemideae) based on nucleotide sequences of nuclear ribosomal DNA internal transcribed spacers (ITS). Pl Biol 5:274–284. https://doi.org/10.1055/s-2003-40790
Wang JT (1988) The steppes and deserts of the Xizang Plateau (Tibet). Vegetatio 75:135–142. https://doi.org/10.1007/BF00045626
Wang G, Zhou G, Yang L, Li Z (2003) Distribution, species diversity and life-form spectra of plant communities along an altitudinal gradient in the northern slopes of Qilianshan Mountains, Gansu, China. Pl Ecol 165:169–181. https://www.jstor.org/stable/20146379
Watson LE, Bates PL, Evans TM, Unwin MM, Estes JR (2002) Molecular phylogeny of Subtribe Artemisiinae (Asteraceae), including Artemisia and its allied and segregate genera. BMC Evol Biol 2:17–28. https://doi.org/10.1186/1471-2148-2-17
Zhao H, Chen F, Chen S, Wu G, Guo W (2010) Molecular phylogeny of Chrysanthemum, Ajania and its allies (Anthemideae, Asteraceae) as inferred from nuclear ribosomal ITS and chloroplast trnL-F IGS sequences. Pl Syst Evol 284:153–169. https://doi.org/10.1007/s00606-009-0242-0
Acknowledgements
The author is very much obliged to the Higher Education Commission (HEC) of Pakistan for providing funds and appreciates the support provided by Dr. Daniel Potter, Professor Department of Plant Sciences, University of California Davis, CA, United States of America.
Funding
This work was funded by Higher Education Commission (HEC) of Pakistan under IRSIP grant (I-8/HEC/HRD/2017/7680/IRSIP/36/BMS/43) awarded to Adil Hussain.
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Hussain, A. Phylogenetic placement of the northeastern Pakistani Ajania fruticulosa (Ledeb.) Poljakov (Asteraceae) on the basis of nrDNA-ITS sequences. Vegetos 36, 999–1007 (2023). https://doi.org/10.1007/s42535-022-00468-2
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DOI: https://doi.org/10.1007/s42535-022-00468-2